Thermo Scientific™
Luminaris Probe qPCR Master Mix, low ROX
Thermo Scientific Luminaris Color Probe and Luminaris Probe qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step Read more
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| Catalog Number | No. of Reactions |
|---|---|
| K0941 | 250 Reactions |
| K0942 | 500 Reactions |
| K0943 | 1250 Reactions |
| K0944 | 5000 Reactions |
Catalog number K0941
Price (USD)/ 250 reactions
282.00
-
No. of Reactions:
250 Reactions
Price (USD)/ 250 reactions
282.00
Luminaris Probe qPCR Master Mix, low ROX
Catalog numberK0941
Price (USD)/ 250 reactions
282.00
-
Thermo Scientific Luminaris Color Probe and Luminaris Probe qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR. The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template, primers and probe need to be added.
The Hot Start Taq DNA polymerase, in combination with an optimized buffer, ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control (see Reference 1).
The use of Luminaris Color Probe and Luminaris Probe qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color Probe and Luminaris Probe qPCR Master Mixes with ROX at different concentrations as passive reference dye are available for most qPCR platforms (see Real-time PCR instrument compatibility chart).
Highlights
• UDG in the master mix to prevent carry-over contamination• Specificity—Hot Start Taq DNA Polymerase and the optimized buffer reduce primer dimers
• Sensitivitity—single copy detection
• Wide linear range—accurate quantification across 9 orders of magnitude
• Reproducibility and convenience— ready-to-use 2X master mix
• Equal performance of blue and colorless master mixes
• Versions with premixed ROX for different real-time platforms
• Standard cycling protocols
Applications
• Gene expression analysis
• siRNA validation
• Genotyping
• Pathogen detection
Includes
Luminaris Probe Low ROX qPCR Master Mix (2X) includes Hot Start Taq Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer. The master mix contains low concentration of ROX passive reference dye for specific real-time platforms (see Real-Time PCR instrument compatibility chart) and is supplied with nuclease-free water.
The Hot Start Taq DNA polymerase, in combination with an optimized buffer, ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control (see Reference 1).
The use of Luminaris Color Probe and Luminaris Probe qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color Probe and Luminaris Probe qPCR Master Mixes with ROX at different concentrations as passive reference dye are available for most qPCR platforms (see Real-time PCR instrument compatibility chart).
Highlights
• UDG in the master mix to prevent carry-over contamination• Specificity—Hot Start Taq DNA Polymerase and the optimized buffer reduce primer dimers
• Sensitivitity—single copy detection
• Wide linear range—accurate quantification across 9 orders of magnitude
• Reproducibility and convenience— ready-to-use 2X master mix
• Equal performance of blue and colorless master mixes
• Versions with premixed ROX for different real-time platforms
• Standard cycling protocols
Applications
• Gene expression analysis
• siRNA validation
• Genotyping
• Pathogen detection
Includes
Luminaris Probe Low ROX qPCR Master Mix (2X) includes Hot Start Taq Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer. The master mix contains low concentration of ROX passive reference dye for specific real-time platforms (see Real-Time PCR instrument compatibility chart) and is supplied with nuclease-free water.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodPrimer-probe
GC-Rich PCR PerformanceHigh
PCR MethodqPCR
PolymeraseTaq DNA Polymerase
Reaction SpeedStandard
For Use With (Equipment)7500 System, QuantStudio™, ViiA™ 7 System, Stratagene Mx4000, Stratagene Mx3000P, Stratagene Mx3005P
Product LineLuminaris
Product TypeqPCR Master Mix
Sufficient For250 Reactions
For Use With (Application)Gene Expression
Concentration2X
Fidelity (vs. Taq)2 X
No. of Reactions250 Reactions
Sample TypeDNA, dsDNA
Unit Size250 reactions
Contents & Storage
Contains:
• 2 x 1.25 mL Luminaris Probe Low ROX qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG and dNTPs (with dUTP) in an optimized PCR buffer; sufficient for 250 x 20 μL reactions. The master mix contains low concentration of ROX passive reference dye for specific real-time platforms.
• 2 x 1.25 mL Nuclease-Free Water
Store at -20°C.
• 2 x 1.25 mL Luminaris Probe Low ROX qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG and dNTPs (with dUTP) in an optimized PCR buffer; sufficient for 250 x 20 μL reactions. The master mix contains low concentration of ROX passive reference dye for specific real-time platforms.
• 2 x 1.25 mL Nuclease-Free Water
Store at -20°C.