Maxima Probe qPCR Master Mix (2X), with separate ROX vial
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Thermo Scientific™

Maxima Probe qPCR Master Mix (2X), with separate ROX vial

**********************Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’veRead more
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Catalog NumberNo. of Reactions
K0261200 Reactions
K02621,000 Reactions
Catalog number K0261
Price (USD)/ 200 reactions
266.00
-
Add to cart
No. of Reactions:
200 Reactions
Price (USD)/ 200 reactions
266.00
Add to cart
Maxima Probe qPCR Master Mix (2X), with separate ROX vial
Catalog numberK0261
Price (USD)/ 200 reactions
266.00
-
Add to cart
**********************
Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of Maxima Probe qPCR Master Mix and added additional capabilities for your gene expression analysis.

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Thermo Scientific Maxima Probe qPCR Master Mixes are ready-to-use solutions optimized for quantitative real-time PCR. The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added.

Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG) (see Reference 1). The use of Maxima Probe qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates.

Maxima Probe qPCR Master Mixes are compatible with most real-time thermal cyclers.

Highlights

• Specificity—Maxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
• Sensitivity—detects low copy number targets
• Wide linear range—accurate quantification across 9 orders of magnitude
• Reproducibility and convenience—ready-to-use 2X master mix

Applications

• Gene expression
• siRNA validation
• Genotyping
• Pathogen detection

Includes
Maxima Probe qPCR Master Mix, ROX Solution provided (2X) includes Maxima Hot Start Taq DNA Polymerase and dNTPs (also dUTP) in an optimized PCR buffer. Supplied with nuclease-free water and a separate ROX vial.

Related Products
Maxima Probe/ROX qPCR Master Mix (2X)
Maxima Probe qPCR Master Mix (2X), with separate ROX vial
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodPrimer-probe
FormatTube
GC-Rich PCR PerformanceHigh
PCR MethodqPCR
PolymeraseTaq DNA Polymerase
Reaction SpeedStandard
For Use With (Equipment)BioRad CFX96™, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, MJ Chromo4, Eppendorf MasterCycler RealPlex, Corbett RotorGene, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, MJ Opticon
Product LineMaxima
Product TypeqPCR Master Mix
Sufficient For200 Reactions of 25 μL
For Use With (Application)Gene Expression
Concentration2X
No. of Reactions200 Reactions
Sample TypeDNA, dsDNA
Volume2 x 1.25 mL
Unit Size200 reactions
Contents & Storage
Contains:
• 2 x 1.25 mL Maxima Probe qPCR Master Mix (2X) containing Maxima Hot Start Taq DNA Polymerase and dNTPs (also dUTP) in an optimized PCR buffer; sufficient for 200 x 25 μL reactions.
• 50 μL ROX solution, 50 μM
• 2 x 1.25 mL Nuclease-Free Water

Store at -20°C.