Orbitrap Exploris™ Isotope Solutions

Open new dimensions in isotope ratio analysis with Thermo Scientific™ Orbitrap Exploris™ Isotope Solutions. Preserve intramolecular isotopic information throughout the analysis and determine previously inaccessible isotopologues by the combination of soft electrospray ionization (ESI) and high-resolution accurate mass (HRAM) sensitivity. The powerful workflow comprises a dedicated sample introduction concept based on Dual Inlet principles, a distinctive method setup, and a data evaluation framework to determine isotope ratio data from the ratios of intact isotopologues of molecular ions.

Technology

Orbitrap Exploris Isotope Solutions dedicated workflow includes:

• Thermo Scientific™ Orbitrap Exploris™ 120/240/480 MS
• Data evaluation Package for Isotope Ratio MS
• Dual syringe inlet
• Optional Thermo Scientific™ Vanquish™ Neo UHPLC System for additional automation

Sample introduction

• Dual Syringe Inlet system utilizing the diverter valve option of the Orbitrap Exploris MS
• In-flow Injection automated approach based on the Vanquish Neo UHPLC System

Analytical capabilities

• Isotope ratios at natural abundance: direct measurements of individual analytes in liquid samples and simultaneous acquisition of all major and some minor isotopologues, enabling abundance determination of ¹³C, ¹⁵N, ¹⁸O, ¹⁷O, ²H, ³⁴S, ³³S, ³⁶S, and more
• Position-specific isotope ratios: controlled fragmentation of organic molecular species that enables unique position-specific isotope analysis
• Clumped isotopes analysis: measurements of isotopologues with multiple rare isotope substitutions

Applications

• Biogeochemical cycles research using multiple isotopologues of CNSOH bearing species (e.g., nitrate, sulfate, phosphate, acetate, and other simple organics)
• Study of geological proxies and their formation (e.g., formation of alkenones and fatty acids)
• Deconvoluting complex metabolic pathways by tracing metabolite transformations
• Food authenticity using isotope ratios of multiple elements and position-specific isotope analysis