Element Search
Structure Search
Thermo Scientific Chemicals
5-Bromo-2'-deoxyuridine, 99%, Thermo Scientific Chemicals
Have Questions?
Change view
Catalog number H27260.06
also known as H27260-06
Price (USD)/ Each
317.65
Online exclusive
373.00 Save 55.35 (15%)
-
Quantity:
5 g
Price (USD)/ Each
317.65
Online exclusive
373.00 Save 55.35 (15%)
5-Bromo-2'-deoxyuridine, 99%, Thermo Scientific Chemicals
Catalog numberH27260.06
Price (USD)/ Each
317.65
Online exclusive
373.00 Save 55.35 (15%)
-
Chemical Identifiers
IUPAC Name5-bromo-1-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]-1,2,3,4-tetrahydropyrimidine-2,4-dione
Molecular FormulaC9H11BrN2O5
InChI KeyWOVKYSAHUYNSMH-RRKCRQDMSA-N
SMILESOC[C@H]1O[C@H](C[C@@H]1O)N1C=C(Br)C(=O)NC1=O
Molecular Weight (g/mol)307.10
View more
Specifications
Specification Sheet
Specification SheetAppearance (Color)White to pale cream
Assay (HPLC)≥98.5%
Optical Rotation+31.0 ± 1.5? (c=1 in 0.1N NaOH) or +23.0 ± 1? (c=1 in water)
FormCrystals or powder or crystalline powder
Identification (FTIR)Conforms (UK sourced product only)
5-Bromo-2'-deoxyuridine (BrdU) is a thymidine analog used to label DNA. It is incorporated into newly synthesized DNA in place of thymidine during the S phase of the cell cycle. Cells that were actively proliferating can then be detected by denaturing the DNA and allowing specific antibodies to target the BrdU incorporation. Consequently 5-BrdU is used to study cell signaling and other processes that induce cell proliferation.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
Applications
5-Bromo-2′-deoxyuridine (BrdU) is a thymidine analog used to label DNA. It is incorporated into newly synthesized DNA in place of thymidine during the S phase of the cell cycle. Cells that were actively proliferating can then be detected by denaturing the DNA and allowing specific antibodies to target the BrdU incorporation. Consequently 5-BrdU is used to study cell signaling and other processes that induce cell proliferation.
Solubility
Soluble in water.
Notes
Store in cool place. Keep container tightly closed in a dry and well-ventilated place. Store away from strong oxidizing agents.
5-Bromo-2′-deoxyuridine (BrdU) is a thymidine analog used to label DNA. It is incorporated into newly synthesized DNA in place of thymidine during the S phase of the cell cycle. Cells that were actively proliferating can then be detected by denaturing the DNA and allowing specific antibodies to target the BrdU incorporation. Consequently 5-BrdU is used to study cell signaling and other processes that induce cell proliferation.
Solubility
Soluble in water.
Notes
Store in cool place. Keep container tightly closed in a dry and well-ventilated place. Store away from strong oxidizing agents.
RUO – Research Use Only
General References:
- Chen Li,; Avrum I. Gotlieb. Transforming Growth Factor-β Regulates the Growth of Valve Interstitial Cellsin Vitro. The American Journal of Pathology. 2011, 179 (4), 1746-1755 .
- Yu Gui et. al. Signaling mechanisms mediating uridine adenosine tetraphosphate-induced proliferation of human vascular smooth muscle cells. Journal of Cardiovascular Pharmacology. 2011 , 58(6), 654-662 .
- Thymidine analog, preferentially incorporated into cellular DNA in place of thymidine, producing increased radiosensitivity of the cell: Int. J. Radiat. Oncol. Biol. Phys., 10, 1399 (1984); 21, 709 (1991). Mutagen used in genetic research: Mutat. Res., 258, 161 (1991). Reviews on use in diagnostics and research: Histochem. J., 27, 339, 923 (1995; 28, 531 (1996).