Glacios™ 2 Cryo-TEM for Life Sciences

Find additional information here: Thermo Scientific™ Glacios™ 2 Cryo Transmission Electron Microscope (Cryo-TEM)

The Thermo Scientific™ Glacios™ 2 Cryo Transmission Electron Microscope (Cryo-TEM) delivers a complete solution for high resolution, high throughput macromolecular structure determination. It features 200 kV XFEG optics, the industry-leading Autoloader (cryogenic sample manipulation robot) and the same innovative automation for ease of use as the Krios G4 Cryo-TEM. The Glacios Cryo-TEM bundles all this into a small footprint that simplifies installation. The designed-in connectivity ensures a robust and contamination-free pathway throughout the entire workflow, from sample preparation and optimization to image acquisition and data processing. The Glacios 2 Cryo-TEM is suited for single-particle analysis, cryo-electron tomography (cryo-ET) and micro-electron diffraction (MicroED).

Key benefits-

High resolution structure determination 

The Glacios 2 Cryo-TEM provides near atomic resolution. With improved hardware and a new enclosure that minimizes environmental influences, the Glacios 2 Cryo-TEM now has an information limit of 2.1 Å. Similarly, transmission loss due to ice growth has been reduced to 2% over 24 hours, helping you collect more high-resolution images with less effort. When Glacios 2 Cryo-TEM is configured with a Thermo Scientific Selectris X Imaging Filter, the speed and resolution can be further improved.

Enhanced ease of use through automation

The Smart EPU software enables faster and easier set-up and processing, benefiting both expert and non-expert microscopists. Several critical set-up steps have been automated, such as alignment of the column and repair of system status, when possible. In addition, the software and various optional add-ons can help users get the most out of their microscope time and the best possible results.

Maximum throughput

EPU is the native software package for automated screening and acquisition of large data sets on the Thermo Fisher cryo-TEMs. With full control of the Autoloader from within EPU, all grids in a cassette can be batch-screened: after the creation of a grid atlas, ice quality (presence, thickness) of the vitrified grids is automatically categorized to support a guided selection of grid squares.

Workflow connectivity

The Glacios 2 Cryo-TEM fits seamlessly into single particle analysis, MicroED and cryo-ET workflows. The Autoloader capsule and cassette systems ensure a robust and contamination-free transfer of samples between Glacios, Talos Arcita, and Krios Cryo-TEMs and Aquilos, Arctis Cryo-FIBs without the need for manipulation of individual small grids. 

Small footprint

The hardware architecture of the Glacios 2 Cryo-TEM has been specifically designed with a smaller footprint and easier access path compared to other 200kV microscopes with automated sample loading systems.