Phusion™ Plus DNA Polymerase
| Catalog Number | No. of Reactions |
|---|---|
| F630XL | 2000 Reactions |
| F630L | 500 Reactions |
| F630S | 100 Reactions |
Features include:
- >100X higher sequence accuracy than Taq polymerase
- Universal annealing temperature of 60°C in PCR
- Hot-start mechanism reduces non-specific amplification and primer degradation
- Automation enabled due to 24-hour stability of assembled reactions
- High inhibitor tolerance for work with DNA of suboptimal purity
The novel Phusion Plus reaction buffer includes proprietary additives that stabilize primer/template duplexes during the annealing step at 60°C. This unique buffer formulation increases PCR specificity without the need to optimize annealing temperature for each primer pair, helping save time and avoid mistakes in PCR runs. Furthermore, with Phusion Plus DNA Polymerase and its universal annealing feature, different PCR reactions can be cycled together using the same protocol.
Phusion Plus DNA Polymerase is inactive at ambient temperatures due to its hot-start mechanism mediated by Affibody molecules, allowing setup and storage of assembled PCR reactions at room temperature. Enzyme activity is fully restored after the initial denaturation step.
Applications
- High-fidelity PCR
- Cloning & mutagenesis
- Template generation for sequencing
- GC-rich PCR
- Long-range PCR (up to 20 kb)
- High-throughput PCR
Notes
- To simplify reactrion setup, Phusion Plus DNA Polymerase is also available in ready-to-use master mix formats.
• Phusion Plus DNA Polymerase (250 μL)
• 5X Phusion Plus Buffer (5 x 1.25 mL)
• 5X Phusion GC Enhancer (4 x 1.25 mL)
Store at –15°C to –25°C.