Thermo Scientific™
Phusion™ High-Fidelity DNA Polymerases (2 U/μL)
Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.
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| Catalog Number | Color | No. of Reactions |
|---|---|---|
| F530L | Colorless | 500 Reactions |
| F530S | Colorless | 100 Reactions |
| F-530XL | Colorless | 2000 Reactions |
| F534S | Green | 100 Reactions |
| F534L | Green | 500 Reactions |
Catalog number F530L
Price (USD)/ 500 units
432.00
Special Offer
Online exclusive
Ends: 16-Dec-2024
540.00 Save 108.00 (20%)
-
Color:
Colorless
No. of Reactions:
500 Reactions
Price (USD)/ 500 units
432.00
Special Offer
Online exclusive
Ends: 16-Dec-2024
540.00 Save 108.00 (20%)
Phusion™ High-Fidelity DNA Polymerases (2 U/μL)
Catalog numberF530L
Price (USD)/ 500 units
432.00
Special Offer
Online exclusive
Ends: 16-Dec-2024
540.00 Save 108.00 (20%)
-
Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is an excellent choice for cloning and other applications requiring high fidelity. Phusion DNA polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerases.
Features of Phusion™ High-Fidelity DNA Polymerase include:
- High fidelity (52X Taq)
- Fast PCR due to short extension times (15–30 s/kb)
- Robust performance, minimal optimization needed
- High yields of PCR products with minimal enzyme amounts
- Available in Green buffer format for direct loading of PCR products on gels (F-534S or F-534L)
Applications
- High-fidelity PCR
- Cloning
- Template generation for sequencing
- Amplification of difficult (GC-rich) templates
- Long-range PCR (up to 20 kb)
- Mutagenesis
- High throughput PCR
- Microarray
Note: Annealing rules for Phusion DNA polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our convenient Tm calculator.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
GC-Rich PCR PerformanceHigh
PolymerasePhusion High-Fidelity DNA Polymerase
Reaction SpeedFast
Product TypeHigh-Fidelity DNA Polymerase
Quantity500 U
Shipping ConditionDry Ice
For Use With (Application)Standard PCR, High-fidelity PCR
Concentration2 U/μL
Fidelity (vs. Taq)52X
Hot StartNo
No. of Reactions500 Reactions
OverhangBlunt
Reaction FormatStandalone
Size (Final Product)20 kb or less
ColorColorless
Unit Size500 units
Contents & Storage
• Phusion DNA Polymerase (2 U/μL)
• 5X Phusion HF & GC Buffers
• DMSO
• 50 mM MgCl2 solution
Phusion HF Buffer and Phusion GC Buffers each provide 1.5 mM MgCl2 in the final 1X concentration.
Store at -20°C.
• 5X Phusion HF & GC Buffers
• DMSO
• 50 mM MgCl2 solution
Phusion HF Buffer and Phusion GC Buffers each provide 1.5 mM MgCl2 in the final 1X concentration.
Store at -20°C.