Phire Tissue Direct PCR Master Mix
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Phire Tissue Direct PCR Master Mix
Thermo Scientific™

Phire Tissue Direct PCR Master Mix

Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects.
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Catalog NumberNo. of Reactions
F170S250 Reactions
F170L1250 Reactions
Catalog number F170S
Price (USD)/ 250 reactions
200.65
Online exclusive
234.00 
Save 33.35 (14%)
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Add to cart
No. of Reactions:
250 Reactions
Request bulk or custom format
Price (USD)/ 250 reactions
200.65
Online exclusive
234.00 
Save 33.35 (14%)
Add to cart
Phire Tissue Direct PCR Master Mix
Catalog numberF170S
Price (USD)/ 250 reactions
200.65
Online exclusive
234.00 
Save 33.35 (14%)
-
Add to cart
Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, humans, fish, birds, and insects. The Master Mix containing Phire Hot Start II DNA Polymerase is specially formulated to perform PCR in the presence of different animal tissue–derived inhibitors such as collagen, melanin and eumelanin (hair, skin), and myoglobin (muscle). The kit also includes DNARelease Additive, which can be used to improve the release of DNA from difficult tissues.

Features

  • Direct PCR reduces time-consuming and expensive DNA purification steps
  • Available Dilution & Storage protocol allows multiple PCR reactions from a single sample
  • Specially engineered Phire DNA polymerase for extremely short PCR protocol times
  • Master mix format with premixed gel loading dye minimizes cross-contamination, reduces sample handling, and allows direct loading on gel

Applications

  • Genotyping
  • Transgene detection
  • Knockout analysis
  • Sequencing

Notes

  • The optimal annealing temperature for Phire DNA Polymerases may differ significantly from that of Taq-based polymerases.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
GC-Rich PCR PerformanceMedium
PCR MethodDirect PCR
PolymerasePhire Hot Start II DNA Polymerase
Reaction SpeedFast
Includes Label or DyeYes
Product TypeDirect PCR Master Mix
Shipping ConditionWet or Dry Ice
For Use With (Application)Genotyping, Hot-start PCR
Concentration2X
Hot StartBuilt-In Hot Start
No. of Reactions250 Reactions
OverhangBlunt
Reaction FormatSuperMix or Master Mix
Sample TypeTissue
Starting MaterialDNA
Unit Size250 reactions
Contents & Storage
• 2X Phire Tissue Direct PCR Master Mix, 2 x 1.25 mL
• Dilution Buffer, 5 mL
• DNARelease Additive, 3 x 100 μL
• 25 μM Control Primer Mix, 40 μL
• Nuclease-free water, 2 x 1.25 mL
• 50 μg O'GeneRuler Express DNA Ladder

Store at -20°C.