Klenow Fragment, exo– (5 U/μL)
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Klenow Fragment, exo– (5 U/μL)
Thermo Scientific™

Klenow Fragment, exo– (5 U/μL)

Thermo Scientific Klenow Fragment, exo-, is the large fragment of DNA polymerase I . It exhibits 5'→3' polymerase activity, butRead more
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Catalog NumberQuantity
EP04221500 U
EP0421300 U
Catalog number EP0422
Price (USD)/ 1,500 units
390.65
Online exclusive
564.00 
Save 173.35 (31%)
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Add to cart
Quantity:
1500 U
Request bulk or custom format
Price (USD)/ 1,500 units
390.65
Online exclusive
564.00 
Save 173.35 (31%)
Add to cart
Klenow Fragment, exo– (5 U/μL)
Catalog numberEP0422
Price (USD)/ 1,500 units
390.65
Online exclusive
564.00 
Save 173.35 (31%)
-
Add to cart
Thermo Scientific Klenow Fragment, exo-, is the large fragment of DNA polymerase I . It exhibits 5'→3' polymerase activity, but lacks the 3'→5' and 5'→3' exonuclease activities of DNA Polymerase I. The 3'→5' exonuclease activity of the enzyme is eliminated by mutations in the 3'→5'-exonuclease active site.

Highlights

• Lacks 3'→5' exonuclease activity
• Incorporates modified nucleotides (e.g., Cy3-, Cy5-, fluorescein-, rhodamine-, aminoallyl-, biotin-labeled nucleotides)
• Active in restriction enzyme, PCR, and RT buffers

Applications
• Random-primed DNA labeling
• Labeling by fill-in 5'-overhangs of dsDNA
• Strand displacement amplification (SDA)
• DNA sequencing by the Sanger method

Note

Klenow Fragment, exo- is not recommended for DNA blunting reactions prior to DNA ligation since it frequently adds one or more extra nucleotides to the 3'-terminus of blunt-end DNA substrates in a non-template directed fashion.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration5 U/μL
PolymeraseDNA Polymerase I
Compatible BufferRestriction Enzyme, PCR, RT Buffer
Quantity1500 U
Product TypeKlenow Fragment
Unit Size1,500 units