Thermo Scientific™
Exonuclease I (20 U/μL)
Thermo Scientific Exonuclease I (ExoI) degrades single-stranded DNA in a 3'→5' direction. It releases deoxyribonucleoside 5'-monophosphates in a stepwise manner Read more
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| Catalog Number | Quantity |
|---|---|
| EN0582 | 20,000 U |
| EN0581 | 4000 U |
Catalog number EN0582
Price (USD)/ 20,000 units
402.65
Online exclusive
454.00 Save 51.35 (11%)
-
Quantity:
20,000 U
Price (USD)/ 20,000 units
402.65
Online exclusive
454.00 Save 51.35 (11%)
Exonuclease I (20 U/μL)
Catalog numberEN0582
Price (USD)/ 20,000 units
402.65
Online exclusive
454.00 Save 51.35 (11%)
-
Thermo Scientific Exonuclease I (ExoI) degrades single-stranded DNA in a 3'→5' direction. It releases deoxyribonucleoside 5'-monophosphates in a stepwise manner and leaves 5'-terminal dinucleotides intact.
It does not cleave DNA strands with terminal 3'-OH groups blocked by phosphoryl or acetyl groups.
Highlights
• Active in PCR buffers
Applications
• Primer removal from PCR mixtures:
• prior to PCR product sequencing
• for one-tube 'megaprimer' PCR mutagenesis
• Removal of single-stranded DNA containing a 3'-hydroxyl terminus from nucleic acid mixtures
• Assay for the presence of single-stranded DNA with a 3'-hydroxyl terminus
Note
The enzyme is not suitable for removing 3'-overhangs of dsDNA.
It does not cleave DNA strands with terminal 3'-OH groups blocked by phosphoryl or acetyl groups.
Highlights
• Active in PCR buffers
Applications
• Primer removal from PCR mixtures:
• prior to PCR product sequencing
• for one-tube 'megaprimer' PCR mutagenesis
• Removal of single-stranded DNA containing a 3'-hydroxyl terminus from nucleic acid mixtures
• Assay for the presence of single-stranded DNA with a 3'-hydroxyl terminus
Note
The enzyme is not suitable for removing 3'-overhangs of dsDNA.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration20 U/μL
EnzymeExonuclease
Quantity20,000 U
Product TypeExonuclease I
Unit Size20,000 units