Brefeldin A (from Penicillium brefeldianum)

The fungal metabolite brefeldin A (BFA) has multiple targets in cells and has proven valuable for dissecting the cellular processes, including vesicle formation and kinesin distribution, involved in exporting newly synthesized proteins. We are pleased to offer the natural brefeldin A isolated from Penicillium brefeldianum. Exposing cells to BFA causes a distortion in intracellular protein traffic from the ER to the Golgi apparatus and the eventual loss of Golgi apparatus morphology; removal of BFA completely reverses these effects. BFA also alters the morphology of endosomes and lysosomes.

Dissolve in high-quality anhydrous DMSO or ethanol to prepare a stock concentration up to 1 mM.