Invitrogen™
BacLight™ Bacterial Membrane Potential Kit
The BacLight™ Bacterial Membrane Potential Kit provides the fluorescent membrane-potential indicator dye, DiOC2(3), along with a proton ionophore (CCCP) and Read more
Catalog number B34950
Price (USD)/ 1 kit
310.65
Online exclusive
332.00 Save 21.35 (6%)
-
Price (USD)/ 1 kit
310.65
Online exclusive
332.00 Save 21.35 (6%)
BacLight™ Bacterial Membrane Potential Kit
Catalog numberB34950
Price (USD)/ 1 kit
310.65
Online exclusive
332.00 Save 21.35 (6%)
-
The BacLight™ Bacterial Membrane Potential Kit provides the fluorescent membrane-potential indicator dye, DiOC2(3), along with a proton ionophore (CCCP) and premixed buffer. DiOC2(3) at low concentrations exhibits green fluorescence in all bacterial cells, but it becomes more concentrated in healthy cells that are maintaining a membrane potential, causing the dye to self-associate and the fluorescence emission to shift to red. The red- and green-fluorescent bacterial populations are easily distinguished using a flow cytometer. CCCP is included in the kit for use as a control because it eradicates the proton gradient, eliminating bacterial membrane potential.
View additional information about all microbiology assays for flow cytometry.
View additional information about all microbiology assays for flow cytometry.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
For Use With (Application)Membrane Potential Assay
For Use With (Equipment)Flow Cytometer
Product TypeBacterial Membrane Potential Kit
Dye TypeMembrane Potential Probes
Emission482⁄497
FormatTube(s)
Product LineBacLight™
Quantity1 kit
Shipping ConditionWet Ice
Unit Size1 kit
Contents & Storage
Contains DiOC2(3) (1.2 mL of 3 mM solution in DMSO), CCCP (300 μL of 500 μM solution in DMSO), and PBS (200 mL). Store in refrigerator (2–8°C) and protect from light.