Invitrogen™
ARCA (Anti-Reverse Cap Analog)
Ambion™ Anti-Reverse Cap Analog (ARCA) is a cap analog used during in vitro transcription for the generation of capped transcripts. Read more
Catalog number AM8045
Price (USD)/ 10 units
324.00
-
Price (USD)/ 10 units
324.00
ARCA (Anti-Reverse Cap Analog)
Catalog numberAM8045
Price (USD)/ 10 units
324.00
-
Ambion™ Anti-Reverse Cap Analog (ARCA) is a cap analog used during in vitro transcription for the generation of capped transcripts. ARCA is modified in a way that ensures incorporation in the forward orientation only. Anti-Reverse Cap Analog (ARCA) is a modified cap analog in which the 3' OH group (closer to m7G) is replaced with –OCH3 (see schematic). Because of this substitution, the RNA polymerase can only initiate transcription with the remaining hydroxyl group thus forcing ARCA incorporation in the forward orientation. As a result, unlike transcripts synthesized with conventional cap analog, 100% of the transcripts synthesized with ARCA at the 5' end are translatable leading to a strong stimulatory effect on translation.
Quality Control:
Each Cap Analog is rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity. Each is also assayed for the efficient synthesis of capped in vitro transcripts by using the cap analog in functional MEGAscript™ In Vitro transcription and Retic Lysate translation reactions.
Accessory Products:
Complete kits are available with all the necessary reagents for the in vitro transcription of capped RNA transcripts. The mMESSAGE mMACHINE™ T7 Ultra Kit includes ARCA (SKU #AM1345). Also available are kits containing conventional cap analog; mMESSAGE mMACHINE™ T7 (SKU #AM1344), T3 (SKU #AM1348), and SP6 (SKU #AM1340) Kits. The Retic Lysate IVT™ (SKU #AM1200) for in vitro translation of mRNA is also available.
Quality Control:
Each Cap Analog is rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity. Each is also assayed for the efficient synthesis of capped in vitro transcripts by using the cap analog in functional MEGAscript™ In Vitro transcription and Retic Lysate translation reactions.
Accessory Products:
Complete kits are available with all the necessary reagents for the in vitro transcription of capped RNA transcripts. The mMESSAGE mMACHINE™ T7 Ultra Kit includes ARCA (SKU #AM1345). Also available are kits containing conventional cap analog; mMESSAGE mMACHINE™ T7 (SKU #AM1344), T3 (SKU #AM1348), and SP6 (SKU #AM1340) Kits. The Retic Lysate IVT™ (SKU #AM1200) for in vitro translation of mRNA is also available.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration40 mM
Final Product TypeCap Analog for In vitro Transcription, Cap Analog for mRNA
For Use With (Application)In Vitro Transcription, synthetic mRNA, mRNA
For Use With (Equipment)mMessage mMAchine, MEGAscript
Labeling MethodRNA Labeling
Product LineAmbion™
Product TypeCap analog for mRNA, cap analog for synthetic mRNA
Quantity10 Units
Shipping ConditionDry Ice
Unit Size10 units
Contents & Storage
Supplied in a single tube to be stored at or below –70°C. Avoid multiple freeze-thaw cycles. Aliquots of the product may be stored short-term at –20°C.