Product References
POM121 promotes the proliferation and metastasis of gastric cancer via PI3K/AKT/MYC pathway.
American journal of cancer research
Kang C,Jia L,Hao L,Zhang N,Liu Y,Zhang L
AM4300 was used in Western Blotting to suggest that POM121 has the potential to act as an independent prognostic factor for GC patients.
Sat Mar 11 00:00:00 EST 2023
Proliferating cell nuclear antigen inhibitors block distinct stages of herpes simplex virus infection.
PLoS pathogens
Packard JE,Williams MR,Fromuth DP,Dembowski JA
AM4300 was used in Western Blotting to conclude that inhibitors of cellular PCNA block recruitment of key viral and cellular factors to viral DNA to inhibit viral DNA synthesis and coupled processes.
Sat Jul 01 00:00:00 EDT 2023
Metformin regulates myoblast differentiation through an AMPK-dependent mechanism.
PloS one
Maniscalco E,Abbadessa G,Giordano M,Grasso L,Borrione P,Racca S
AM4300 was used in Western Blotting to provide solid evidence that most of the effects of Met on myoblasts and myotubes are mediated by AMPK.
Tue Feb 14 00:00:00 EST 2023
N6-Methyladenosine RNA Modification in Host Cells Regulates Peste des Petits Ruminants Virus Replication.
Microbiology spectrum
Khan O,Tanuj GN,Choravada DR,Rajak KK,Chandra Sekar S,Lingaraju MC,Dhara SK,Gupta PK,Mishra BP,Dutt T,Gandham RK,Sajjanar B
AM4300 was used in Western Blot to show that the m6A modification of RNA regulates PPRV replication.
Tue Feb 14 00:00:00 EST 2023
Cysteine dioxygenase and taurine are essential for embryo implantation by involving in E2-ERα and P4-PR signaling in mouse.
Journal of animal science and biotechnology
Zhang D,Wang Z,Luo X,Guo H,Qiu G,Gong Y,Gao H,Cui S
AM4300 was used in Western Blotting to demonstrate that taurine and CDO play prominent roles in uterine receptivity and embryo implantation by involving in E2-ERα and P4-PR signaling.
Thu Jan 05 00:00:00 EST 2023
Nanoparticle-mediated targeting of the fusion gene RUNX1/ETO in t(8;21)-positive acute myeloid leukaemia.
Leukemia
Issa H,Swart LE,Rasouli M,Ashtiani M,Nakjang S,Jyotsana N,Schuschel K,Heuser M,Blair H,Heidenreich O
AM4300 was used in Western Blotting to provide proof for the feasibility of targeting RUNX1/ETO in a pre-clinical setting and support the further development of siRNA-LNPs for the treatment of fusion gene-driven malignancies.
Sat Apr 01 00:00:00 EDT 2023
N-glycoproteomics reveals distinct glycosylation alterations in NGLY1-deficient patient-derived dermal fibroblasts.
Journal of inherited metabolic disease
Budhraja R,Saraswat M,De Graef D,Ranatunga W,Ramarajan MG,Mousa J,Kozicz T,Pandey A,Morava E
AM4300 was used in Western Blotting to highlight new molecular and system-level insights for understanding NGLY1-CDDG.
Sun Jan 01 00:00:00 EST 2023
Filamin A organizes γ‑aminobutyric acid type B receptors at the plasma membrane.
Nature communications
Jobin ML,Siddig S,Koszegi Z,Lanoiselée Y,Khayenko V,Sungkaworn T,Werner C,Seier K,Misigaiski C,Mantovani G,Sauer M,Maric HM,Calebiro D
AM4300 was used in Cell Culture to show that GABAB receptors are located together with filamin A in small nanodomains in hippocampal neurons.
Tue Jan 03 00:00:00 EST 2023
Polarity signaling balances epithelial contractility and mechanical resistance.
Scientific reports
Rübsam M,Püllen R,Tellkamp F,Bianco A,Peskoller M,Bloch W,Green KJ,Merkel R,Hoffmann B,Wickström SA,Niessen CM
AM4300 was used in Western Blotting to indicate that keratins sense the contractile stress state of stratified epithelia and balance increased contractility by mounting a protective response to maintain tissue integrity.
Fri May 12 00:00:00 EDT 2023
Therapeutic effect of human ApoA-I-Milano variant in aged transgenic mouse model of Alzheimer's disease.
British journal of pharmacology
Solé M,Marazuela P,Castellote L,Bonaterra-Pastra A,Giménez-Llort L,Hernández-Guillamon M
AM4300 was used in Western Blotting to show the potential therapeutic applicability of a safe and non-invasive treatment based on peripheral administration of hrApoA-I-M in Alzheimer's disease.
Tue Aug 01 00:00:00 EDT 2023
COL7A1 Expression Improves Prognosis Prediction for Patients with Clear Cell Renal Cell Carcinoma Atop of Stage.
Cancers
Koca D,Séraudie I,Jardillier R,Cochet C,Filhol O,Guyon L
AM4300 was used in Western Blotting to identify COL7A1 as a new prognosis marker that can stratify ccRCC patients.
Wed May 10 00:00:00 EDT 2023
Blood transcriptomic signatures associated with molecular changes in the brain and clinical outcomes in Parkinson's disease.
Nature communications
Irmady K,Hale CR,Qadri R,Fak J,Simelane S,Carroll T,Przedborski S,Darnell RB
AM4300 was used in Western Blotting to identify molecular signatures in Parkinson's disease patients' brain and blood of potential pathophysiologic and prognostic importance.
Wed Jul 05 00:00:00 EDT 2023
The long non-coding RNA LINC00707 interacts with Smad proteins to regulate TGFβ signaling and cancer cell invasion.
Cell communication and signaling : CCS
Gélabert C,Papoutsoglou P,Golán I,Ahlström E,Ameur A,Heldin CH,Caja L,Moustakas A
AM4300 was used in Western Blot to investigate the mechanism of action and biological role of Transforming growth factor β (TGFβ) signaling via Smad proteins in cancer.
Mon Oct 02 00:00:00 EDT 2023
Substrate stiffness induces nuclear localization of myosin regulatory light chain to suppress apoptosis.
FEBS letters
Onishi K,Ishihara S,Takahashi M,Sakai A,Enomoto A,Suzuki K,Haga H
AM4300 was used in Western Blotting to reveal a newly identified role of MRLC in mechanotransduction.
Wed Mar 01 00:00:00 EST 2023
Upregulation of the ESCRT pathway and multivesicular bodies accelerates degradation of proteins associated with neurodegeneration.
Autophagy reports
Benyair R,Giridharan SSP,Rivero-Ríos P,Hasegawa J,Bristow E,Eskelinen EL,Shmueli MD,Fishbain-Yoskovitz V,Merbl Y,Sharkey LM,Paulson HL,Hanson PI,Patnaik S,Al-Ramahi I,Botas J,Marugan J,Weisman LS
AM4300 was used in Western Blotting to reveal that upregulation of traffic through an ESCRT-dependent MVB pathway may provide a therapeutic approach for neurodegenerative diseases.
Wed Apr 19 00:00:00 EDT 2023
Upregulation of the ESCRT pathway and multivesicular bodies accelerates degradation of proteins associated with neurodegeneration.
Autophagy reports
Benyair R,Giridharan SSP,Rivero-Ríos P,Hasegawa J,Bristow E,Eskelinen EL,Shmueli MD,Fishbain-Yoskovitz V,Merbl Y,Sharkey LM,Paulson HL,Hanson PI,Patnaik S,Al-Ramahi I,Botas J,Marugan J,Weisman LS
AM4300 was used in Western Blotting to reveal that upregulation of traffic through an ESCRT-dependent MVB pathway may provide a therapeutic approach for neurodegenerative diseases.
Wed Apr 19 00:00:00 EDT 2023
Intrinsic D614G and P681R/H mutations in SARS-CoV-2 VoCs Alpha, Delta, Omicron and viruses with D614G plus key signature mutations in spike protein alters fusogenicity and infectivity.
Medical microbiology and immunology
Khatri R,Siddqui G,Sadhu S,Maithil V,Vishwakarma P,Lohiya B,Goswami A,Ahmed S,Awasthi A,Samal S
AM4300 was used in Western Blotting to reveal that D614G and P681R/H mutations in spike protein favor VoC replication in different host compartments, thus allowing a balance of mutation vs selection for better long-term adaptation.
Wed Feb 01 00:00:00 EST 2023
HAMLET effect on cell death and mitochondrial respiration in colorectal cancer cell lines with KRAS/BRAF mutations.
Journal of cancer research and clinical oncology
Žilinskas J,Stukas D,Jasukaitienė A,Šapauskienė J,Banienė R,Trumbeckaitė S,Švagždys S,Cicciu M,Dambrauskas Ž,Gulbinas A,Tamelis A
AM4300 was used in Western Blotting to investigate HAMLET effect on viability, death pathway and mitochondrial bioenergetics of CRC cells with different KRAS/BRAF mutational status in vitro.
Fri Sep 01 00:00:00 EDT 2023
Molecular basis for ubiquitin/Fubi cross-reactivity in USP16 and USP36.
Nature chemical biology
O'Dea R,Kazi N,Hoffmann-Benito A,Zhao Z,Recknagel S,Wendrich K,Janning P,Gersch M
AM4300 was used in Western Blotting to highlight how ubiquitin/Fubi specificity is achieved in a subset of human deubiquitinases and open the door to a systematic investigation of the Fubi system.
Wed Nov 01 00:00:00 EDT 2023
Mercury increases IL-1β and IL-18 secretion and intensifies coronary arteritis in an animal model of Kawasaki disease.
Frontiers in immunology
Alphonse MP,Duong TT,Tam S,Yeung RSM
AM4300 was used in Western Blotting to study the effect of mercury on inflammasome activation and its impact on the immunopathogenesis of Kawasaki disease (KD).
Tue May 02 00:00:00 EDT 2023
The endosomal system of primary human vascular endothelial cells and albumin-FcRn trafficking.
Journal of cell science
Pannek A,Becker-Gotot J,Dower SK,Verhagen AM,Gleeson PA
AM4300 was used in Western Blotting to investigate FcRn-albumin trafficking in physiologically relevant endothelial cells.
Tue Aug 01 00:00:00 EDT 2023
Ras-mutant cancers are sensitive to small molecule inhibition of V-type ATPases in mice.
Nature biotechnology
Tolani B,Celli A,Yao Y,Tan YZ,Fetter R,Liem CR,de Smith AJ,Vasanthakumar T,Bisignano P,Cotton AD,Seiple IB,Rubinstein JL,Jost M,Weissman JS
AM4300 was used in Flow cytometry/Cell sorting to establish proof-of-concept for targeting V-ATPase in cancers driven by specific KRAS mutations such as KRASG13D and G12V.
Thu Dec 01 00:00:00 EST 2022
The caspase-6-p62 axis modulates p62 droplets based autophagy in a dominant-negative manner.
Cell death and differentiation
Valionyte E,Yang Y,Griffiths SA,Bone AT,Barrow ER,Sharma V,Lu B,Luo S
AM4300 was used in Western Blot to show that p62-droplet formation is selectively blocked by inflammatory toxicity, which induces cleavage of p62 by caspase-6 at a novel cleavage site D256.
Wed Jun 01 00:00:00 EDT 2022
Genome-wide CRISPR screen identifies regulators of MAPK and MTOR pathways mediating sorafenib resistance in acute myeloid leukemia.
Haematologica
Damnernsawad A,Bottomly D,Kurtz SE,Eide CA,McWeeney SK,Tyner JW,Nechiporuk T
AM4300 was used in Western Blot to show negative regulators of the MAPK and MTOR pathways LZTR1, NF1, TSC1/TSC2 are mediators of sorafenib resistance.
Sat Jan 01 00:00:00 EST 2022
MiR-146b-5p regulates IL-23 receptor complex expression in chronic lymphocytic leukemia cells.
Blood advances
Matis S,Grazia Recchia A,Colombo M,Cardillo M,Fabbi M,Todoerti K,Bossio S,Fabris S,Cancila V,Massara R,Reverberi D,Emionite L,Cilli M,Cerruti G,Salvi S,Bet P,Pigozzi S,Fiocca R,Ibatici A,Angelucci E,Gentile M,Monti P,Menichini P,Fronza G,Torricelli F,Ciarrocchi A,Neri A,Fais F,Tripodo C,Morabito F,Ferrarini M,Cutrona G
AM4300 was used in Flow cytometry/Cell sorting to indicate that IL-12β1 expression, a crucial checkpoint for the functioning of the IL-23/IL-23R complex loop, is under the control of miR-146b-5p, which may represent a potential target for therapy since it contributes to the CLL pathogenesis.
Tue Oct 25 00:00:00 EDT 2022
Aberrant stromal tissue factor localisation and mycolactone-driven vascular dysfunction, exacerbated by IL-1β, are linked to fibrin formation in Buruli ulcer lesions.
PLoS pathogens
Hsieh LT,Dos Santos SJ,Hall BS,Ogbechi J,Loglo AD,Salguero FJ,Ruf MT,Pluschke G,Simmonds RE
AM4300 was used in Western Blot to explore the role of the vasculature in Buruli ulcer pathogenesis.
Sat Jan 01 00:00:00 EST 2022
Hepatocytes Deficient in Nuclear Envelope Protein Lamina-associated Polypeptide 1 are an Ideal Mammalian System to Study Intranuclear Lipid Droplets.
Journal of lipid research
Östlund C,Hernandez-Ono A,Turk SJ,Dauer WT,Ginsberg HN,Worman HJ,Shin JY
AM4300 was used in Western Blotting to show that LAP1-depleted hepatocytes represent an ideal mammalian system to investigate the biogenesis of nuclear LDs and their partitioning between the nucleus and cytoplasm in response to changes in nutritional state and cellular metabolism in vivo.
Sat Oct 01 00:00:00 EDT 2022
Prolactin Reduces Hippocampal Parvalbumin and GABAA Receptor Expression in Female Mice.
Neuroendocrinology
Mellado S,Moreno-Ruiz B,Expósito S,Fernández M,Martín ED
AM4300 was used in Western Blotting to study whether pituitary hormones are related to parvalbumin (PV) expression in the hippocampus demonstrating that increased prolactin (PRL) levels are associated with changes in key constituent elements of inhibitory circuits in the hippocampus.
Thu Aug 04 00:00:00 EDT 2022
Study on Toll-Like Receptor 2-Mediated Inflammation-Induced Familial Hypertension Combined with Hyperlipemia and Its Mechanism.
Journal of healthcare engineering
Liu J,Li C,Wang Q,Hu H,Li C,Qian J
AM4300 was used in Western Blot to investigate the influence of Toll-like receptor 2- (TLR2-) mediated inflammation on the occurrence and development of familial hypertension combined with hyperlipemia and its related mechanism.
Thu Apr 21 00:00:00 EDT 2022
Downregulation of low-density lipoprotein receptor mRNA in lymphatic endothelial cells impairs lymphatic function through changes in intracellular lipids.
Theranostics
Vachon L,Smaani A,Tessier N,Jean G,Demers A,Milasan A,Ardo N,Jarry S,Villeneuve L,Alikashani A,Finherty V,Ruiz M,Sorci-Thomas MG,Mayer G,Martel C
AM4300 was used in Western Blot to suggest that treatments that specifically palliate the down regulation of LDLR mRNA in lymphatic endothelial cells preserve the integrity of the lymphatic endothelium and sustain lymphatic function, a prerequisite player in atherosclerosis.
Thu Apr 21 00:00:00 EDT 2022
Multiple 9-1-1 complexes promote homolog synapsis, DSB repair, and ATR signaling during mammalian meiosis.
eLife
Pereira C,Arroyo-Martinez GA,Guo MZ,Downey MS,Kelly ER,Grive KJ,Mahadevaiah SK,Sims JR,Faca VM,Tsai C,Schiltz CJ,Wit N,Jacobs H,Clark NL,Freire R,Turner J,Lyndaker AM,Brieno-Enriquez MA,Cohen PE,Smolka MB,Weiss RS
AM4300 was used in Immunohistochemistry, Western Blot to establish critical roles for both canonical and alternative 9-1-1 complexes in meiotic ATR activation and successful prophase I completion.
Tue Feb 08 00:00:00 EST 2022
Multiple 9-1-1 complexes promote homolog synapsis, DSB repair, and ATR signaling during mammalian meiosis.
eLife
Pereira C,Arroyo-Martinez GA,Guo MZ,Downey MS,Kelly ER,Grive KJ,Mahadevaiah SK,Sims JR,Faca VM,Tsai C,Schiltz CJ,Wit N,Jacobs H,Clark NL,Freire R,Turner J,Lyndaker AM,Brieno-Enriquez MA,Cohen PE,Smolka MB,Weiss RS
AM4300 was used in Immunohistochemistry, Western Blot to establish critical roles for both canonical and alternative 9-1-1 complexes in meiotic ATR activation and successful prophase I completion.
Tue Feb 08 00:00:00 EST 2022
Histone deacetylase HDAC2 regulates microRNA-125a expression in neuroblastoma.
Brain and behavior
Liu D,Tang X,Huang Z,Wen J,Zhou Y
AM4300 was used in Western Blotting to ascertain whether miR-125a exerts antitumor effects in NB.
Tue Feb 01 00:00:00 EST 2022
Missense mutations in inositol 1,4,5-trisphosphate receptor type 3 result in leaky Ca2+ channels and activation of store-operated Ca2+ entry.
iScience
Terry LE,Arige V,Neumann J,Wahl AM,Knebel TR,Chaffer JW,Malik S,Liston A,Humblet-Baron S,Bultynck G,Yule DI
AM4300 was used in Western Blotting to demonstrate that a common feature of these mutations is decreased IP3R3 function and that these mutations exhibit a novel phenotype manifested as a constitutively open channel, which inappropriately gates SOCE in the absence of stimulation.
Thu Dec 22 00:00:00 EST 2022
Inactivation of PTEN and ZFHX3 in Mammary Epithelial Cells Alters Patterns of Collective Cell Migration.
International journal of molecular sciences
Dayoub A,Fokin AI,Lomakina ME,James J,Plays M,Jacquin T,Novikov NM,Vorobyov RS,Schegoleva AA,Rysenkova KD,Gaboriaud J,Leonov SV,Denisov EV,Gautreau AM,Alexandrova AY
AM4300 was used in Western Blotting to generate single and combined PTEN and ZFHX3 knock-outs (KOs) in the immortalized mammary epithelial cell line MCF10A to study the role of these genes and their potential synergy in migration regulation.
Sat Dec 24 00:00:00 EST 2022
MEK-inhibitor-mediated rescue of skeletal myopathy caused by activating Hras mutation in a Costello syndrome mouse model.
Disease models & mechanisms
Tidyman WE,Goodwin AF,Maeda Y,Klein OD,Rauen KA
AM4300 was used in Western Blotting to investigate the mechanisms underlying hypotonia in Costello syndrome.
Tue Feb 01 00:00:00 EST 2022
m6A Modification Involves in Enriched Environment-Induced Neurogenesis and Cognition Enhancement.
Frontiers in cell and developmental biology
Qu W,Li Q,Wang M,Zhao X,Wu J,Liu D,Hong S,Yang Y,Shu Q,Li X
AM4300 was used in Western Blotting to study the environment exposure modification of neurogenesis.
Sat Jul 16 00:00:00 EDT 2022
NOX4 regulates TGFβ-induced proliferation and self-renewal in glioblastoma stem cells.
Molecular oncology
García-Gómez P,Golán I,S Dadras M,Mezheyeuski A,Bellomo C,Tzavlaki K,Morén A,Carreras-Puigvert J,Caja L
AM4300 was used in Western Blotting to establish NOX4 as a key mediator of GSC biology.
Sun May 01 00:00:00 EDT 2022
Alteration of ribosome function upon 5-fluorouracil treatment favors cancer cell drug-tolerance.
Nature communications
Therizols G,Bash-Imam Z,Panthu B,Machon C,Vincent A,Ripoll J,Nait-Slimane S,Chalabi-Dchar M,Gaucherot A,Garcia M,Laforêts F,Marcel V,Boubaker-Vitre J,Monet MA,Bouclier C,Vanbelle C,Souahlia G,Berthel E,Albaret MA,Mertani HC,Prudhomme M,Bertrand M,David A,Saurin JC,Bouvet P,Rivals E,Ohlmann T,Guitton J,Dalla Venezia N,Pannequin J,Catez F,Diaz JJ
AM4300 was used in Western Blot to show that 5-fluorouracil treatment leads to the production of fluorinated ribosomes exhibiting altered translational activities.
Mon Jan 10 00:00:00 EST 2022
Ets1 mediates sorafenib resistance by regulating mitochondrial ROS pathway in hepatocellular carcinoma.
Cell death & disease
Vishnoi K,Ke R,Viswakarma N,Srivastava P,Kumar S,Das S,Singh SK,Principe DR,Rana A,Rana B
AM4300 was used in Western Blotting to indicate the activation of a novel Ets-1-GPX2 signaling axis in soraR cells, targeting which might successfully antagonize resistance and increase efficacy.
Mon Jul 04 00:00:00 EDT 2022
The HIV-1 Viral Protease Is Activated during Assembly and Budding Prior to Particle Release.
Journal of virology
Tabler CO,Wegman SJ,Chen J,Shroff H,Alhusaini N,Tilton JC
AM4300 was used in Western Blotting to find that protease is fully activated before virus is released from the cell membrane, which is hours earlier than recent estimates.
Wed May 11 00:00:00 EDT 2022
Nestin Modulates Airway Smooth Muscle Cell Migration by Affecting Spatial Rearrangement of Vimentin Network and Focal Adhesion Assembly.
Cells
Wang R,Khan S,Liao G,Wu Y,Tang DD
AM4300 was used in Western Blotting to suggest that nestin promotes smooth muscle cell migration.
Thu Sep 29 00:00:00 EDT 2022
Increased atrial effectiveness of flecainide conferred by altered biophysical properties of sodium channels.
Journal of molecular and cellular cardiology
O' Brien S,Holmes AP,Johnson DM,Kabir SN,O' Shea C,O' Reilly M,Avezzu A,Reyat JS,Hall AW,Apicella C,Ellinor PT,Niederer S,Tucker NR,Fabritz L,Kirchhof P,Pavlovic D
AM4300 was used in Western Blotting to highlight differences in biophysical properties of sodium channels in left atria and ventricles and their response to flecainide.
Sun May 01 00:00:00 EDT 2022
Hypoxia-Inducible Factor-1α in Rods Is Neuroprotective Following Retinal Detachment.
Investigative ophthalmology & visual science
Ross BX,Jia L,Kong D,Wang T,Yao J,Hager HM,Abcouwer SF,Zacks DN
AM4300 was used in Western Blotting to determine the necessity of HIF-1α on photoreceptors cell survival after retinal detachment.
Mon Oct 03 00:00:00 EDT 2022
High-titer neutralization of Mu and C.1.2 SARS-CoV-2 variants by vaccine-elicited antibodies of previously infected individuals.
Cell reports
Tada T,Zhou H,Dcosta BM,Samanovic MI,Cornelius A,Herati RS,Mulligan MJ,Landau NR
AM4300 was used in Western Blot to study the infectivity of SARS-CoV-2 variants Mu, C.1.2, and Delta+N501S spike proteins and determine their susceptibility to neutralization by convalescent and vaccine-elicited antibodies, both in previously infected and uninfected individuals.
Tue Jan 11 00:00:00 EST 2022
MYC amplifies gene expression through global changes in transcription factor dynamics.
Cell reports
Patange S,Ball DA,Wan Y,Karpova TS,Girvan M,Levens D,Larson DR
AM4300 was used in Western Blotting to propose that the mechanism by which MYC exerts global effects on the active period of genes is by altering the binding dynamics of transcription factors involved in RNA polymerase II complex assembly and productive elongation.
Tue Jan 25 00:00:00 EST 2022
Changes in adenosine receptors and neurotrophic factors in the SOD1G93A mouse model of amyotrophic lateral sclerosis: Modulation by chronic caffeine.
PloS one
Rei N,Valente CA,Vaz SH,Farinha-Ferreira M,Ribeiro JA,Sebastião AM
AM4300 was used in Western Blotting to study an early dysfunction of the adenosinergic system in ALS and suggest the possibility that the negative influence of caffeine previously reported in ALS animal models results from interference with BDNF rather than with the VEGF signaling molecules.
Fri Dec 16 00:00:00 EST 2022
Transcription factor antagonism regulates heterogeneity in embryonic stem cell states.
Molecular cell
Hu S,Metcalf E,Mahat DB,Chan L,Sohal N,Chakraborty M,Hamilton M,Singh A,Singh A,Lees JA,Sharp PA,Garg S
AM4300 was used in Western Blotting to show antagonism between factors at enhancers results in gene expression heterogeneity and formation of cell states, with implications for the generation of diverse cell types during development.
Thu Dec 01 00:00:00 EST 2022
Epithelial SMYD5 Exaggerates IBD by Down-regulating Mitochondrial Functions via Post-Translational Control of PGC-1α Stability.
Cellular and molecular gastroenterology and hepatology
Hou Y,Sun X,Gheinani PT,Guan X,Sharma S,Zhou Y,Jin C,Yang Z,Naren AP,Yin J,Denning TL,Gewirtz AT,Liu Y,Xie Z,Li C
AM4300 was used in Western Blotting to investigate the role and underlying mechanism of epithelial SMYD5 in IBD pathogenesis and progression.
Mon Jul 25 00:00:00 EDT 2022
Short-term and bystander effects of radiation on murine submandibular glands.
Disease models & mechanisms
Uchida H,Ingalls MH,Maruyama EO,Johnston CJ,Hernady E,Faustoferri RC,Ovitt CE
AM4300 was used in Western Blotting to investigate the mechanisms of cellular damage induced by radiation treatment.
Tue Nov 01 00:00:00 EDT 2022
NAD+ Modulates the Proliferation and Differentiation of Adult Neural Stem/Progenitor Cells via Akt Signaling Pathway.
Cells
Huang X,Guo H,Cheng X,Zhang J,Qu W,Ding Q,Sun Q,Shu Q,Li X
AM4300 was used in Western Blotting to show that nicotinamide adenine dinucleotide hydrate (NAD+) exposure leads to the reduced proliferation and neuronal differentiation of adult neural stem/progenitor cells (aNSPCs) and induces the apoptosis of aNSPCs.
Sat Apr 09 00:00:00 EDT 2022
Involvement of CaV 2.2 channels and α2 δ-1 in homeostatic synaptic plasticity in cultured hippocampal neurons.
The Journal of physiology
Pilch KS,Ramgoolam KH,Dolphin AC
AM4300 was used in Western Blotting to reveal a key role for CaV 2.2 channels and novel roles for α2 δ-1 during synaptic plastic adaptation.
Thu Dec 01 00:00:00 EST 2022
The Purkinje-myocardial junction is the anatomic origin of ventricular arrhythmia in CPVT.
JCI insight
Blackwell DJ,Faggioni M,Wleklinski MJ,Gomez-Hurtado N,Venkataraman R,Gibbs CE,Baudenbacher FJ,Gong S,Fishman GI,Boyle PM,Pfeifer K,Knollmann BC
AM4300 was used in Western Blot to conclude that ectopic beats in CPVT are likely generated at the Purkinje-myocardial junction via a heretofore unrecognized tissue mechanism, whereby DADs in the ventricular myocardium trigger full action potentials in adjacent Purkinje cells.
Tue Feb 08 00:00:00 EST 2022
Phyllanthus taxodiifolius Beille Disrupted N-cadherin, Vimentin, Paxillin and Actin Stress Fibers in Glioblastoma.
Asian Pacific journal of cancer prevention : APJCP
Sunrat C,Kwanthongdee J,Uppakara K,Chabang N,Munyoo B,Tuchinda P,Saengsawang W
AM4300 was used in Western Blotting to explore the effects of Phyllanthus taxodiifolius Beille extract on molecules known to be hallmarks of aggressive glioblastoma including N-cadherin and vimentin, mesenchymal markers, and paxillin.
Fri Jul 01 00:00:00 EDT 2022
TRIM7 Restricts Coxsackievirus and Norovirus Infection by Detecting the C-Terminal Glutamine Generated by 3C Protease Processing.
Viruses
Luptak J,Mallery DL,Jahun AS,Albecka A,Clift D,Ather O,Slodkowicz G,Goodfellow I,James LC
AM4300 was used in Western Blotting to show that TRIM7's extreme substrate promiscuity is due to a highly unusual binding mechanism.
Sat Jul 23 00:00:00 EDT 2022
Microarray Analysis Reveals Overexpression of both Integral Membrane and Cytosolic Tight Junction Genes in Endometrial Cancer Cell Lines.
Journal of Cancer
Cuevas ME,Winters CP,Todd MC
AM4300 was used in Western Blotting to suggest that the structural disruption of tight junction coupled with the downstream deregulation of signaling pathways involved in cellular proliferation and migration may contribute to the development of endometrial cancer.
Sat Dec 10 00:00:00 EST 2022
CK2β Is a Gatekeeper of Focal Adhesions Regulating Cell Spreading.
Frontiers in molecular biosciences
Filhol O,Hesse AM,Bouin AP,Albigès-Rizo C,Jeanneret F,Battail C,Pflieger D,Cochet C
AM4300 was used in Western Blotting to show that decreased expression of CK2β in MCF10A mammary epithelial cells triggers the phosphorylation of a number of proteins on tyrosine residues and promotes the striking activation of the FAK1-Src-PAX1 signaling pathway.
Tue Jul 19 00:00:00 EDT 2022
GS-CA1 and lenacapavir stabilize the HIV-1 core and modulate the core interaction with cellular factors.
iScience
Selyutina A,Hu P,Miller S,Simons LM,Yu HJ,Hultquist JF,Lee K,KewalRamani VN,Diaz-Griffero F
AM4300 was used in Western Blot to show that GS-CA1 binds to the HIV-1 core, changes its conformation, stabilizes the core, and thereby prevents viral uncoating and infection.
Fri Jan 21 00:00:00 EST 2022
Loss of Fas Receptor Function Preserves Photoreceptor Structure and Function in Two Mouse Models of Inherited Retinal Degeneration.
Investigative ophthalmology & visual science
Yao J,Wang T,Jia L,Qiu Y,Zacks DN
AM4300 was used in Immunohistochemistry (Paraffin) to conclude that reducing Fas activity might represent a potential mutation-independent therapeutic approach to preserve retinal structure and function in patients with inherited retinal degeneration (IRD).
Thu Sep 01 00:00:00 EDT 2022
Mammaglobin 1 mediates progression of trastuzumab-resistant breast cancer cells through regulation of cyclins and NF-κB.
FEBS open bio
Kusumastuti R,Kumagai Y,Ishihara S,Enomoto A,Murakami T,Yasuda M,Haga H
AM4300 was used in Western Blotting to indicate that MGB1 expression is increased in breast cancer cells that have gained resistance to trastuzumab, and suggest that MGB1 promotes aggressiveness through cyclin and NF-κB regulation.
Sat Oct 01 00:00:00 EDT 2022
Structure and function of the N-terminal extension of the formin INF2.
Cellular and molecular life sciences : CMLS
Labat-de-Hoz L,Comas L,Rubio-Ramos A,Casares-Arias J,Fernández-Martín L,Pantoja-Uceda D,Martín MT,Kremer L,Jiménez MA,Correas I,Alonso MA
AM4300 was used in Immunocytochemistry, Western Blot to show that the N-terminal extension of INF2 is organized into two α-helices, the first of which is necessary to maintain the perinuclear F-actin ring and normal cytosolic F-actin content.
Fri Oct 28 00:00:00 EDT 2022
Structure and function of the N-terminal extension of the formin INF2.
Cellular and molecular life sciences : CMLS
Labat-de-Hoz L,Comas L,Rubio-Ramos A,Casares-Arias J,Fernández-Martín L,Pantoja-Uceda D,Martín MT,Kremer L,Jiménez MA,Correas I,Alonso MA
AM4300 was used in Immunocytochemistry, Western Blot to show that the N-terminal extension of INF2 is organized into two α-helices, the first of which is necessary to maintain the perinuclear F-actin ring and normal cytosolic F-actin content.
Fri Oct 28 00:00:00 EDT 2022
Induction of osteogenesis by bone-targeted Notch activation.
eLife
Xu C,Dinh VV,Kruse K,Jeong HW,Watson EC,Adams S,Berkenfeld F,Stehling M,Rasouli SJ,Fan R,Chen R,Bedzhov I,Chen Q,Kato K,Pitulescu ME,Adams RH
AM4300 was used in Immunohistochemistry to propose that activation of Notch signaling by bone-targeted fusion proteins might be therapeutically useful and can avoid detrimental effects in Notch-dependent processes in other organs.
Fri Feb 04 00:00:00 EST 2022
PD-L1 promotes myofibroblastic activation of hepatic stellate cells by distinct mechanisms selective for TGF-β receptor I versus II.
Cell reports
Sun L,Wang Y,Wang X,Navarro-Corcuera A,Ilyas S,Jalan-Sakrikar N,Gan C,Tu X,Shi Y,Tu K,Liu Q,Lou Z,Dong H,Sharpe AH,Shah VH,Kang N
AM4300 was used in Functional Assay to conclude that myofibroblast PD-L1 can modulate the tumor microenvironment and tumor growth by a mechanism independent of immune suppression.
Tue Feb 08 00:00:00 EST 2022
Oncogenic BRAF induces whole-genome doubling through suppression of cytokinesis.
Nature communications
Darp R,Vittoria MA,Ganem NJ,Ceol CJ
AM4300 was used in Western Blotting to investigate if BRAFV600E, the most common mutation in melanomas, can induce whole-genome doubling via cytokinesis failure in vitro and in a zebrafish melanoma model.
Fri Jul 15 00:00:00 EDT 2022
Deep learning for de-convolution of Smad2 versus Smad3 binding sites.
BMC genomics
Ng JWK,Ong EHQ,Tucker-Kellogg L,Tucker-Kellogg G
AM4300 was used in Western Blotting to identify Smad-specific binding sites in a cancer cell line.
Wed Jul 20 00:00:00 EDT 2022
Cardiac troponin T and autoimmunity in skeletal muscle aging.
GeroScience
Zhang T,Feng X,Dong J,Xu Z,Feng B,Haas KM,Cawthon PM,Beavers KM,Nicklas B,Kritchevsky S
AM4300 was used in Western Blotting to suggest a novel cTnT-mediated autoimmune response may be an indicator of sarcopenia.
Mon Aug 01 00:00:00 EDT 2022
A bioinformatic analysis study of m7G regulator-mediated methylation modification patterns and tumor microenvironment infiltration in glioblastoma.
BMC cancer
Wu X,Li C,Wang Z,Zhang Y,Liu S,Chen S,Chen S,Liu W,Liu X
AM4300 was used in Western Blotting to show establishment of a prognostic signature and the extended analysis of the tumor microenvironment, immune correlation, and tumor mutation burden further suggest the important role of m7G in the development and development of this disease.
Mon Jul 04 00:00:00 EDT 2022
High expression of NFX1-123 in HPV positive head and neck squamous cell carcinomas.
Head & neck
Chintala S,Quist KM,Gonzalez-DeWhitt PA,Katzenellenbogen RA
AM4300 was used in Western Blotting to show that NFX1-123 and a subset of its known targets were increased in HPV+ HNSCCs.
Sat Jan 01 00:00:00 EST 2022
A novel, essential trans-splicing protein connects the nematode SL1 snRNP to the CBC-ARS2 complex.
Nucleic acids research
Fasimoye RY,Spencer REB,Soto-Martin E,Eijlers P,Elmassoudi H,Brivio S,Mangana C,Sabele V,Rechtorikova R,Wenzel M,Connolly B,Pettitt J,Müller B
AM4300 was used in Western Blotting to identify a novel, nematode-specific protein required for SL1 trans-splicing, which we designate SNA-3.
Fri Jul 22 00:00:00 EDT 2022
Aporphine and isoquinoline derivatives block glioblastoma cell stemness and enhance temozolomide cytotoxicity.
Scientific reports
Rodrigues-Junior DM,Raminelli C,Hassanie H,Trossini GHG,Perecim GP,Caja L,Moustakas A,Vettore AL
AM4300 was used in Western Blotting to reveal a potential therapeutic application of A5 and C1 as cytotoxic agents against GBM cells and predicted molecular networks that can be further exploited to characterize the pharmacological effects of these isoquinoline-containing substances.
Wed Dec 07 00:00:00 EST 2022
Systemic LSD1 Inhibition Prevents Aberrant Remodeling of Metabolism in Obesity.
Diabetes
Ramms B,Pollow DP,Zhu H,Nora C,Harrington AR,Omar I,Gordts PLSM,Wortham M,Sander M
AM4300 was used in Western Blotting to suggest that targeting LSD1 could be a strategy for treatment of obesity and its associated complications including type 2 diabetes and NAFLD.
Thu Dec 01 00:00:00 EST 2022
Sex-specific differences in KCC2 localisation and inhibitory synaptic transmission in the rat hippocampus.
Scientific reports
Wolf DC,Sanon NT,Cunha AOS,Chen JS,Shaker T,Elhassan AR,do Nascimento ASF,Di Cristo G,Weil AG
AM4300 was used in Western Blotting to show that perinatal testosterone levels modulate GABAergic circuit function, suggesting a critical role of perinatal sex hormones in regulating network excitability in the adult hippocampus.
Thu Feb 24 00:00:00 EST 2022
miRNome Profiling and Functional Analysis Reveal Involvement of hsa-miR-1246 in Colon Adenoma-Carcinoma Transition by Targeting AXIN2 and CFTR.
International journal of molecular sciences
Lukosevicius R,Juzenas S,Salteniene V,Kulokiene U,Arstikyte J,Hemmrich-Stanisak G,Franke A,Link A,Ruzgys P,Satkauskas S,Pauzas H,Latkauskas T,Kiudelis G,Balaguer F,Kupcinskas J,Skieceviciene J
AM4300 was used in Western Blotting to reveal involvement of hsa-miR-1246 in early colorectal cancer development and regulation of tumor suppressors AXIN2 and CFTR.
Mon Feb 14 00:00:00 EST 2022
Serine 13 of the human cytomegalovirus viral cyclin-dependent kinase UL97 is required for regulatory protein 14-3-3 binding and UL97 stability.
The Journal of biological chemistry
Iwahori S,Umaña AC,Kalejta RF,Murata T
AM4300 was used in Western Blotting to conclude that 14-3-3 proteins interact with and stabilize UL97 during HCMV infection.
Tue Nov 01 00:00:00 EDT 2022
Pressure and stiffness sensing together regulate vascular smooth muscle cell phenotype switching.
Science advances
Swiatlowska P,Sit B,Feng Z,Marhuenda E,Xanthis I,Zingaro S,Ward M,Zhou X,Xiao Q,Shanahan C,Jones GE,Yu CH,Iskratsch T
AM4300 was used in Western Blotting to show how different pathological mechanical signals combined but through distinct pathways accelerate a phenotypic switch that will ultimately contribute to atherosclerotic disease progression.
Fri Apr 15 00:00:00 EDT 2022
Pressure and stiffness sensing together regulate vascular smooth muscle cell phenotype switching.
Science advances
Swiatlowska P,Sit B,Feng Z,Marhuenda E,Xanthis I,Zingaro S,Ward M,Zhou X,Xiao Q,Shanahan C,Jones GE,Yu CH,Iskratsch T
AM4300 was used in Western Blotting to show how different pathological mechanical signals combined but through distinct pathways accelerate a phenotypic switch that will ultimately contribute to atherosclerotic disease progression.
Fri Apr 15 00:00:00 EDT 2022
Empagliflozin mitigates endothelial inflammation and attenuates endoplasmic reticulum stress signaling caused by sustained glycocalyx disruption.
Scientific reports
Campeau MA,Leask RL
AM4300 was used in Immunocytochemistry, Western Blot, Immunoprecipitation to demonstrate the potential of EMPA to resolve the inflammatory response of ECs caused by sustained GCX disruption while altering UPR signaling under endoplasmic reticulum stress.
Mon Jul 25 00:00:00 EDT 2022
The Actin Cytoskeleton Responds to Inflammatory Cues and Alters Macrophage Activation.
Cells
Ronzier E,Laurenson AJ,Manickam R,Liu S,Saintilma IM,Schrock DC,Hammer JA,Rotty JD
AM4300 was used in Western Blotting to suggest that interplay between myosin II and Arp2/3 influences macrophage activity, and potentially impacts adaptive-innate immune coordination.
Tue May 31 00:00:00 EDT 2022
Aldosterone-Induced Sarco/Endoplasmic Reticulum Ca2+ Pump Upregulation Counterbalances Cav1.2-Mediated Ca2+ Influx in Mesenteric Arteries.
Frontiers in physiology
Salazar-Enciso R,Guerrero-Hernández A,Gómez AM,Benitah JP,Rueda A
AM4300 was used in Western Blotting to provide novel evidence for short-term ALDO-induced upregulation of the functional unit comprising Cav1.2, SERCA2 pump, RyRs, and BKCa channels; in which the SERCA pump buffers ALDO-induced upregulation of Ca2+ entry at the superficial SR-PM nanodomain of MASMCs, preventing ALDO-triggered depolarization-induced vasoconstriction and enhancing vasodilation.
Sat Apr 02 00:00:00 EDT 2022
LUBAC assembles a ubiquitin signaling platform at mitochondria for signal amplification and transport of NF-κB to the nucleus.
The EMBO journal
Wu Z,Berlemann LA,Bader V,Sehr DA,Dawin E,Covallero A,Meschede J,Angersbach L,Showkat C,Michaelis JB,Münch C,Rieger B,Namgaladze D,Herrera MG,Fiesel FC,Springer W,Mendes M,Stepien J,Barkovits K,Marcus K,Sickmann A,Dittmar G,Busch KB,Riedel D,Brini M,Tatzelt J,Cali T,Winklhofer KF
AM4300 was used in Western Blot to reveal a role for mitochondria in amplifying TNF-mediated NF-κB activation, both serving as a signaling platform, as well as a transport mode for activated NF-κB to the nuclear.
Thu Dec 15 00:00:00 EST 2022
Astronauts Plasma-Derived Exosomes Induced Aberrant EZH2-Mediated H3K27me3 Epigenetic Regulation of the Vitamin D Receptor.
Frontiers in cardiovascular medicine
Bisserier M,Brojakowska A,Saffran N,Rai AK,Lee B,Coleman M,Sebastian A,Evans A,Mills PJ,Addya S,Arakelyan A,Garikipati VNS,Hadri L,Goukassian DA
AM4300 was used in Western Blotting to suggest that astronaut-derived small extracellular vesicles may epigenetically repress the expression of the vitamin D receptor (VDR) in human adult cardiomyocytes by promoting the activation of the PRC2 complex and H3K27me3 levels.
Sat Aug 13 00:00:00 EDT 2022
Specialist multidisciplinary input maximises rare disease diagnoses from whole genome sequencing.
Nature communications
Macken WL,Falabella M,McKittrick C,Pizzamiglio C,Ellmers R,Eggleton K,Woodward CE,Patel Y,Labrum R,Phadke R,Reilly MM,DeVile C,Sarkozy A,Footitt E,Davison J,Rahman S,Houlden H,Bugiardini E,Quinlivan R,Hanna MG,Vandrovcova J,Pitceathly RDS
AM4300 was used in Western Blotting to show that specialist multidisciplinary analysis of whole genome sequencing (WGS), following an initial 'no primary findings' (NPF) report, improves diagnostic rates and alters management.
Mon Nov 07 00:00:00 EST 2022
Parkin regulates adiposity by coordinating mitophagy with mitochondrial biogenesis in white adipocytes.
Nature communications
Moore TM,Cheng L,Wolf DM,Ngo J,Segawa M,Zhu X,Strumwasser AR,Cao Y,Clifford BL,Ma A,Scumpia P,Shirihai OS,Vallim TQA,Laakso M,Lusis AJ,Hevener AL,Zhou Z
AM4300 was used in Western Blotting to indicate that Parkin regulates mitochondrial homeostasis by balancing mitophagy and Pgc1α-mediated mitochondrial biogenesis in white adipocytes, suggesting a potential therapeutic target in adipocytes to combat obesity and obesity-associated disorders.
Fri Nov 04 00:00:00 EDT 2022
β-Elemene Attenuates Renal Fibrosis in the Unilateral Ureteral Obstruction Model by Inhibition of STAT3 and Smad3 Signaling via Suppressing MyD88 Expression.
International journal of molecular sciences
Sun W,Kim DH,Byon CH,Choi HI,Park JS,Bae EH,Ma SK,Kim SW
AM4300 was used in Western Blotting to elucidate the anti-interstitial fibrosis effect of β-elemene, which provides a new direction for future research and development of drugs related to chronic kidney disease.
Mon May 16 00:00:00 EDT 2022
A stem cell roadmap of ribosome heterogeneity reveals a function for RPL10A in mesoderm production.
Nature communications
Genuth NR,Shi Z,Kunimoto K,Hung V,Xu AF,Kerr CH,Tiu GC,Oses-Prieto JA,Salomon-Shulman REA,Axelrod JD,Burlingame AL,Loh KM,Barna M
AM4300 was used in Western Blot to reveal unexpected ribosome composition modularity that controls differentiation and development through the specialized translation of key signaling networks.
Mon Sep 19 00:00:00 EDT 2022
Proteotoxic stress-induced apoptosis in cancer cells: understanding the susceptibility and enhancing the potency.
Cell death discovery
Iuliano L,Dalla E,Picco R,Mallavarapu S,Minisini M,Malavasi E,Brancolini C
AM4300 was used in Western Blotting to determine proteotoxic stress response abilities of leiomyosarcoma.
Tue Oct 04 00:00:00 EDT 2022
High-fat diet disrupts REG3γ and gut microbial rhythms promoting metabolic dysfunction.
Cell host & microbe
Frazier K,Kambal A,Zale EA,Pierre JF,Hubert N,Miyoshi S,Miyoshi J,Ringus DL,Harris D,Yang K,Carroll K,Hermanson JB,Chlystek JS,Overmyer KA,Cham CM,Musch MW,Coon JJ,Chang EB,Leone VA
AM4300 was used in Western Blotting to report the host C-type lectin antimicrobial peptide Reg3γ works with key ileal microbes to orchestrate these interactions in a bidirectional manner and does not correlate with the intestinal core circadian clock.
Wed Jun 08 00:00:00 EDT 2022
Glioblastomas acquire myeloid-affiliated transcriptional programs via epigenetic immunoediting to elicit immune evasion.
Cell
Gangoso E,Southgate B,Bradley L,Rus S,Galvez-Cancino F,McGivern N,Güç E,Kapourani CA,Byron A,Ferguson KM,Alfazema N,Morrison G,Grant V,Blin C,Sou I,Marques-Torrejon MA,Conde L,Parrinello S,Herrero J,Beck S,Brandner S,Brennan PM,Bertone P,Pollard JW,Quezada SA,Sproul D,Frame MC,Serrels A,Pollard SM
AM4300 was used in Western Blot to conclude that epigenetic immunoediting may drive an acquired immune evasion program in the most aggressive mesenchymal GBM subtype by reshaping the tumor immune microenvironment.
Thu Apr 29 00:00:00 EDT 2021
Neutrophils interact with cholangiocytes to cause cholestatic changes in alcoholic hepatitis.
Gut
Takeuchi M,Vidigal PT,Guerra MT,Hundt MA,Robert ME,Olave-Martinez M,Aoki S,Khamphaya T,Kersten R,Kruglov E,de la Rosa Rodriguez R,Banales JM,Nathanson MH,Weerachayaphorn J
AM4300 was used in Western Blotting to examine whether bile ducts participate in the pathogenesis of Alcoholic hepatitis.
Mon Feb 01 00:00:00 EST 2021
Acetyl-CoA Synthetase 2: A Critical Linkage in Obesity-Induced Tumorigenesis in Myeloma.
Cell metabolism
Li Z,Liu H,He J,Wang Z,Yin Z,You G,Wang Z,Davis RE,Lin P,Bergsagel PL,Manasanch EE,Wong STC,Esnaola NF,Chang JC,Orlowski RZ,Yi Q,Yang J
AM4300 was used in Western Blot to demonstrate a key impact for obesity-induced acetyl-CoA synthetase 2 on the progression of myeloma.
Tue Jan 05 00:00:00 EST 2021
Liquid-like protein interactions catalyse assembly of endocytic vesicles.
Nature cell biology
Day KJ,Kago G,Wang L,Richter JB,Hayden CC,Lafer EM,Stachowiak JC
AM4300 was used in Western Blot to show that Eps15 and Fcho1/2 rely on weak, liquid-like interactions to catalyse endocytosis.
Thu Apr 01 00:00:00 EDT 2021
Targeting microRNA-mediated gene repression limits adipogenic conversion of skeletal muscle mesenchymal stromal cells.
Cell stem cell
Wosczyna MN,Perez Carbajal EE,Wagner MW,Paredes S,Konishi CT,Liu L,Wang TT,Walsh RA,Gan Q,Morrissey CS,Rando TA
AM4300 was used in Western Blotting to provide insight into the underlying molecular mechanisms of fibroadipogenic progenitors fate determination and formation of harmful fatty deposits in skeletal muscle.
Thu Jul 01 00:00:00 EDT 2021
GIMAP5 maintains liver endothelial cell homeostasis and prevents portal hypertension.
The Journal of experimental medicine
Drzewiecki K,Choi J,Brancale J,Leney-Greene MA,Sari S,Dalgiç B,Ünlüsoy Aksu A,Evirgen Şahin G,Ozen A,Baris S,Karakoc-Aydiner E,Jain D,Kleiner D,Schmalz M,Radhakrishnan K,Zhang J,Hoebe K,Su HC,Pereira JP,Lenardo MJ,Lifton RP,Vilarinho S
AM4300 was used in Western Blot to identify genetic causes of portal hypertension that occur in the absence of primary disease of the liver parenchyma.
Mon Jul 05 00:00:00 EDT 2021
Identification of a dynamic gene regulatory network required for pluripotency factor-induced reprogramming of mouse fibroblasts and hepatocytes.
The EMBO journal
Papathanasiou M,Tsiftsoglou SA,Polyzos AP,Papadopoulou D,Valakos D,Klagkou E,Karagianni P,Pliatska M,Talianidis I,Agelopoulos M,Thanos D
AM4300 was used in Western Blot to investigate the early transcriptional events of cellular reprogramming triggered by the co-expression of Oct4, Sox2, Klf4, and c-Myc (OSKM) in mouse embryonic fibroblasts (MEFs) and mouse hepatocytes (mHeps).
Mon Jan 04 00:00:00 EST 2021
Identification of novel lipid droplet factors that regulate lipophagy and cholesterol efflux in macrophage foam cells.
Autophagy
Robichaud S,Fairman G,Vijithakumar V,Mak E,Cook DP,Pelletier AR,Huard S,Vanderhyden BC,Figeys D,Lavallée-Adam M,Baetz K,Ouimet M
AM4300 was used in Western Blotting to identify several LD-associated proteins of the lipophagy machinery, a finding with important biological and therapeutic implications.
Mon Nov 01 00:00:00 EDT 2021
The K63 deubiquitinase CYLD modulates autism-like behaviors and hippocampal plasticity by regulating autophagy and mTOR signaling.
Proceedings of the National Academy of Sciences of the United States of America
Colombo E,Horta G,Roesler MK,Ihbe N,Chhabra S,Radyushkin K,Di Liberto G,Kreutzfeldt M,Schumann S,von Engelhardt J,Merkler D,Behl C,Mittmann T,Clement AM,Waisman A,Schmeisser MJ
AM4300 was used in Western Blotting to better understand if CYLD plays a role in brain and synapse homeostasis.
Tue Nov 23 00:00:00 EST 2021
A recombinant herpes virus expressing influenza hemagglutinin confers protection and induces antibody-dependent cellular cytotoxicity.
Proceedings of the National Academy of Sciences of the United States of America
Kaugars K,Dardick J,de Oliveira AP,Weiss KA,Lukose R,Kim J,Leung L,Rajagopalan S,Wolin S,Akabas L,Knipe DM,Bajic G,Jacobs WR
AM4300 was used in Western Blot to develop antibody-dependent cellular cytotoxicity-eliciting influenza vaccine.
Tue Aug 24 00:00:00 EDT 2021
P-selectin targeted RAGE-shRNA lipoplexes alleviate atherosclerosis-associated inflammation.
Journal of controlled release : official journal of the Controlled Release Society
Mocanu CA,Fuior EV,Voicu G,Rebleanu D,Safciuc F,Deleanu M,Fenyo IM,Escriou V,Manduteanu I,Simionescu M,Calin M
AM4300 was used in Western Blot to develop targeted lipoplexes carrying the receptor for advanced glycation end products (RAGE)-short hairpin RNA to down-regulate endothelial RAGE expression as a therapeutic strategy for atherosclerosis.
Sun Oct 10 00:00:00 EDT 2021
The cytoskeleton actin binding protein filamin A impairs both IGF2 mitogenic effects and the efficacy of IGF1R inhibitors in adrenocortical cancer cells.
Cancer letters
Catalano R,Giardino E,Treppiedi D,Mangili F,Morelli V,Elli FM,Serban AL,Luconi M,Mannelli M,Spada A,Arosio M,Mantovani G,Peverelli E
AM4300 was used in Western Blot to test the involvement of cytoskeleton actin-binding protein filamin A in regulating IGF1R and IR responsiveness to both insulin-like growth factor 2 (IGF2) and inhibitors in adrenocortical carcinomas.
Thu Jan 28 00:00:00 EST 2021
AXL Is a Potential Target for the Treatment of Intestinal Fibrosis.
Inflammatory bowel diseases
Steiner CA,Rodansky ES,Johnson LA,Berinstein JA,Cushing KC,Huang S,Spence JR,Higgins PDR
AM4300 was used in Western Blot to study the AXL pathway in activated myofibroblasts implicated in the pathogenesis of fibrosis during Crohn's-related intestinal failure.
Tue Feb 16 00:00:00 EST 2021
BRG1 knockdown inhibits proliferation through multiple cellular pathways in prostate cancer.
Clinical epigenetics
Giles KA,Gould CM,Achinger-Kawecka J,Page SG,Kafer GR,Rogers S,Luu PL,Cesare AJ,Clark SJ,Taberlay PC
AM4300 was used in Western Blot to investigate the molecular effects on global transcription programmes.
Wed Feb 17 00:00:00 EST 2021
The exocyst complex regulates C. elegans germline stem cell proliferation by controlling membrane Notch levels.
Development (Cambridge, England)
Pushpa K,Dagar S,Kumar H,Pathak D,Mylavarapu SVS
AM4300 was used in Western Blotting to reveal that the exocyst complex regulates C. elegans GSC proliferation by modulating Notch signaling cell autonomously.
Sun Aug 01 00:00:00 EDT 2021
Human Hematopoietic Stem, Progenitor, and Immune Cells Respond Ex Vivo to SARS-CoV-2 Spike Protein.
Stem cell reviews and reports
Ropa J,Cooper S,Capitano ML,Van't Hof W,Broxmeyer HE
AM4300 was used in Western Blotting to examine the expression of ACE2, to which SARS-CoV-2 Spike (S) protein binds to facilitate viral entry, in cord blood derived HSCs/HPCs and in peripheral blood derived immune cell subtypes.
Mon Feb 01 00:00:00 EST 2021
Multiplexed Simian Immunodeficiency Virus-Specific Paired RNA-Guided Cas9 Nickases Inactivate Proviral DNA.
Journal of virology
Smith LM,Ladner JT,Hodara VL,Parodi LM,Harris RA,Callery JE,Lai Z,Zou Y,Raveedran M,Rogers J,Giavedoni LD
AM4300 was used in Western Blotting to demonstrate that Cas9 nickase in association with gRNA pairs can specifically eliminate parts of the integrated provirus DNA and show that careful design of an all-in-one plasmid coding for 3 gRNAs and Cas9 nuclease inhibits SIV production with undetectable off-target mutations, making these tools a desirable prospect for moving into animal studies.
Tue Nov 09 00:00:00 EST 2021
Efficient generation of osteoclasts from human induced pluripotent stem cells and functional investigations of lethal CLCN7-related osteopetrosis.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research
Rössler U,Hennig AF,Stelzer N,Bose S,Kopp J,Søe K,Cyganek L,Zifarelli G,Ali S,von der Hagen M,Strässler ET,Hahn G,Pusch M,Stauber T,Izsvák Z,Gossen M,Stachelscheid H,Kornak U
AM4300 was used in Western Blot to demonstrate that osteoclasts differentiated from ARO hiPSCs can be used as a disease model for ARO and potentially also other osteoclast-related diseases.
Sun Aug 01 00:00:00 EDT 2021
Zbtb46-dependent altered developmental program in embryonic stem cell-derived blood cell progenitors.
Stem cells (Dayton, Ohio)
Boto P,Gerzsenyi TB,Lengyel A,Szunyog B,Szatmari I
AM4300 was used in Western Blot to study the effect of the Zbtb46 transcription factor in embryonic stem cell (ESC)-derived blood cell progenitors using chemically inducible mouse cell lines.
Fri Oct 01 00:00:00 EDT 2021
Promotion of Bone Lesions Through the Myeloma Integrin α6-Mediated Osteolytic Signaling.
Frontiers in oncology
Liu H,Wang Z,He J,Li Z,Gao JY,Liu R,Lin P,Yang J
AM4300 was used in Western Blot to demonstrate the role of myeloma cell-expressed integrin α6 in bone.
Tue Jun 22 00:00:00 EDT 2021
A lamin A/C variant causing striated muscle disease provides insights into filament organization.
Journal of cell science
Kronenberg-Tenga R,Tatli M,Eibauer M,Wu W,Shin JY,Bonne G,Worman HJ,Medalia O
AM4300 was used in Western Blot to investigate nuclear lamina molecular changes occurring in cardiomyopathy and muscular dystrophy using a mouse model.
Mon Mar 22 00:00:00 EDT 2021
A general role for TANGO1, encoded by MIA3, in secretory pathway organization and function.
Journal of cell science
McCaughey J,Stevenson NL,Mantell JM,Neal CR,Paterson A,Heesom K,Stephens DJ
AM4300 was used in Western Blot to support a general role for MIA3/TANGO1 in maintaining secretory pathway structure and function in vertebrate cells.
Wed Sep 01 00:00:00 EDT 2021
A cytosolic reductase pathway is required for efficient N-glycosylation of an STT3B-dependent acceptor site.
Journal of cell science
van Lith M,Pringle MA,Fleming B,Gaeta G,Im J,Gilmore R,Bulleid NJ
AM4300 was used in Western Blot to study the redox effects of efficient glycosylation in cellular pathways and protein folding.
Mon Nov 15 00:00:00 EST 2021
Triple deletion of TP53, PCNT, and CEP215 promotes centriole amplification in the M phase.
Cell cycle (Georgetown, Tex.)
Jung GI,Rhee K
AM4300 was used in Western Blotting to reveal the heterogeneity of the supernumerary centrioles in the triple KO cells.
Sun Aug 01 00:00:00 EDT 2021
RGS10 physically and functionally interacts with STIM2 and requires store-operated calcium entry to regulate pro-inflammatory gene expression in microglia.
Cellular signalling
Wendimu MY,Alqinyah M,Vella S,Dean P,Almutairi F,Davila-Rivera R,Rayatpisheh S,Wohlschlegel J,Moreno S,Hooks SB
AM4300 was used in Western Blotting to identify non-canonical RGS10 interacting partners that mediate its anti-inflammatory mechanism.
Thu Jul 01 00:00:00 EDT 2021
An epilepsy-associated ACTL6B variant captures neuronal hyperexcitability in a human induced pluripotent stem cell model.
Journal of neuroscience research
Ahn LY,Coatti GC,Liu J,Gumus E,Schaffer AE,Miranda HC
AM4300 was used in Western Blotting to investigate the molecular pathogenesis of ACTL6B p.(Val421_Cys425del) using in silico 3D protein modeling predictions and patient-specific induced pluripotent stem cell-derived neurons.
Fri Jan 01 00:00:00 EST 2021
Expanding the clinical and metabolic phenotype of DPM2 deficient congenital disorders of glycosylation.
Molecular genetics and metabolism
Radenkovic S,Fitzpatrick-Schmidt T,Byeon SK,Madugundu AK,Saraswat M,Lichty A,Wong SYW,McGee S,Kubiak K,Ligezka A,Ranatunga W,Zhang Y,Wood T,Friez MJ,Clarkson K,Pandey A,Jones JR,Morava E
AM4300 was used in Western Blot to add to the clinical phenotype of DPM2-congenital disorders of glycosylation and add more insights into the pathomechanism of DPM2-CDG disorder through glycomics and lipidomics analysis.
Fri Jan 01 00:00:00 EST 2021
microRNAs (miR 9, 124, 155 and 224) transdifferentiate mouse macrophages to neurons.
Experimental cell research
Challagundla N,Agrawal-Rajput R
AM4300 was used in Western Blot to report a novel approach of microRNA (miR)- induced trans-differentiation of macrophages (CD11b high) to induced neuronal cells (iNCs) (neuronal markershigh- Nestin, Nurr1, Map2, NSE, Tubb3 and Mash1) without exogenous use of transcription factors. miR 9, 124, 155 and 224 successfully transdifferentiated macrophages to neurons with transient stem cell-like phenotype.
Sat May 01 00:00:00 EDT 2021
Curcumin induces apoptosis by inhibiting BCAT1 expression and mTOR signaling in cytarabine‑resistant myeloid leukemia cells.
Molecular medicine reports
Tseng YH,Yang RC,Chiou SS,Shieh TM,Shih YH,Lin PC
AM4300 was used in Western Blot to investigate whether curcumin induces apoptosis by regulating BCAT1 expression.
Sun Aug 01 00:00:00 EDT 2021
Integrin α4β1 is required for IL-1α- and Nrf2-dependent, Cox-2 induction in fibroblasts, supporting a mechanism that suppresses α-SMA expression.
Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society
Zheng R,Varney SD,Wu L,DiPersio CM,Van De Water L
AM4300 was used in Western Blotting to suggest that fibroblast integrin α4β1 regulates-depending on microenvironmental cues-the differentiated state of fibroblasts through a signalling network in which IL-1α, Cox-2 and Nrf2 participate.
Thu Jul 01 00:00:00 EDT 2021
Force dependent effects of chronic overuse on fibrosis-related genes and proteins in skeletal muscles.
Connective tissue research
Hilliard BA,Amin M,Popoff SN,Barbe MF
AM4300 was used in Western Blot to examine the chronic effect of force on mRNA and protein expression levels of fibrosis-related genes in flexor digitorum muscles in a rat model of repetitive overuse injury that induces muscle fibrosis at high force levels.
Fri Jan 01 00:00:00 EST 2021
High Caloric Diet Induces Memory Impairment and Disrupts Synaptic Plasticity in Aged Rats.
Current issues in molecular biology
Paulo SL,Miranda-Lourenço C,Belo RF,Rodrigues RS,Fonseca-Gomes J,Tanqueiro SR,Geraldes V,Rocha I,Sebastião AM,Xapelli S,Diógenes MJ
AM4300 was used in Western Blotting to aim at unravelling the impact of a chronic high caloric diet (HCD) on memory performance and synaptic plasticity in aged rats.
Sat Dec 18 00:00:00 EST 2021
Restricted Water Intake Adversely Affects Rat Vocal Fold Biology.
The Laryngoscope
Duan C,do Nascimento NC,Calve S,Cox A,Sivasankar MP
AM4300 was used in Western Blot to investigate the dehydrating effects of restricted water intake on vocal fold tissue in an in vivo model.
Thu Apr 01 00:00:00 EDT 2021
Catechol-O-methyltransferase and Pregnancy Outcome: an Appraisal in Rat.
Reproductive sciences (Thousand Oaks, Calif.)
Iqbal K,Dhakal P,Pierce SH,Soares MJ
AM4300 was used in Western Blotting to investigate Catechol-O-methyltransferase in rat, a species with deep intrauterine trophoblast invasion.
Mon Feb 01 00:00:00 EST 2021
Inhibition of the canonical Wnt signaling pathway by a β-catenin/CBP inhibitor prevents heart failure by ameliorating cardiac hypertrophy and fibrosis.
Scientific reports
Methatham T,Tomida S,Kimura N,Imai Y,Aizawa K
AM4300 was used in Western Blot to investigate how ICG001 can prevent cardiac hypertrophy and fibrosis induced by transverse aortic constriction (TAC).
Wed Jul 21 00:00:00 EDT 2021
Protein farnesylation is upregulated in Alzheimer's human brains and neuron-specific suppression of farnesyltransferase mitigates pathogenic processes in Alzheimer's model mice.
Acta neuropathologica communications
Jeong A,Cheng S,Zhong R,Bennett DA,Bergö MO,Li L
AM4300 was used in Western Blot to suggest that aberrant upregulation of protein farnesylation is an early driving force in the pathogenic cascade of AD and that targeting FT or its downstream signaling pathways presents a viable therapeutic strategy against AD.
Tue Jul 27 00:00:00 EDT 2021
Acute depletion of CTCF rewires genome-wide chromatin accessibility.
Genome biology
Xu B,Wang H,Wright S,Hyle J,Zhang Y,Shao Y,Niu M,Fan Y,Rosikiewicz W,Djekidel MN,Peng J,Lu R,Li C
AM4300 was used in Western Blotting to determine the effects of CTCF on chromatin topology using Cut&Run.
Tue Aug 24 00:00:00 EDT 2021
Image-based high-throughput mapping of TGF-β-induced phosphocomplexes at a single-cell level.
Communications biology
Lönn P,Al-Amin RA,Doulabi EM,Heldin J,Gallini R,Björkesten J,Oelrich J,Kamali-Moghaddam M,Landegren U
AM4300 was used in Western Blot to investigate cell cycle progression and local cell crowding in relation to TGF-β-responsive Smad2 linker phosphorylations and complex formations over time.
Fri Nov 12 00:00:00 EST 2021
Generation and analysis of innovative genomically humanized knockin SOD1, TARDBP (TDP-43), and FUS mouse models.
iScience
Devoy A,Price G,De Giorgio F,Bunton-Stasyshyn R,Thompson D,Gasco S,Allan A,Codner GF,Nair RR,Tibbit C,McLeod R,Ali Z,Noda J,Marrero-Gagliardi A,Brito-Armas JM,Williams C,Öztürk MM,Simon M,O'Neill E,Bryce-Smith S,Harrison J,Atkins G,Corrochano S,Stewart M,Gilthorpe JD,Teboul L,Acevedo-Arozena A,Fisher EMC,Cunningham TJ
AM4300 was used in Western Blot to establish a new standard of large knockin allele quality control, demonstrating the utility of indirect capture for enrichment of a genomic region of interest followed by Oxford Nanopore sequencing.
Fri Dec 17 00:00:00 EST 2021
Evidence That a TRPA1-Mediated Murine Model of Temporomandibular Joint Pain Involves NLRP3 Inflammasome Activation.
Pharmaceuticals (Basel, Switzerland)
Kodji X,Kee Z,McKenna R,de Sousa Valente J,Ravenscroft H,McMillan H,Gamble J,Dombrowski Y,Moynagh P,Brough D,Lundy FT,Brain SD,El Karim IA
AM4300 was used in Western Blot to investigate the role of transient receptor potential ankyrin 1 (TRPA1) in murine temporomandibular joint (TMJ) inflammatory hyperalgesia and the influence of the NLR family pyrin domain-containing 3 (NLRP3) inflammasome.
Sat Oct 23 00:00:00 EDT 2021
Macrophages Demonstrate Guanylate-Binding Protein-Dependent and Bacterial Strain-Dependent Responses to Francisella tularensis.
Frontiers in cellular and infection microbiology
Mohammadi N,Lindgren H,Yamamoto M,Martin A,Henry T,Sjöstedt A
AM4300 was used in Western Blot to assess the responses of bone marrow derived murine macrophages and guanylate-binding protein deficient bone marrow derived murine macrophages to Francisella tularensis strains of variable virulence.
Fri Jan 28 00:00:00 EST 2022
Human Cytomegalovirus UL138 Protein Inhibits the STING Pathway and Reduces Interferon Beta mRNA Accumulation during Lytic and Latent Infections.
mBio
Albright ER,Mickelson CK,Kalejta RF
AM4300 was used in Western Blotting to show that the latency-associated protein UL138 inhibits the cGAS/STING/TBK1 innate immunity pathway during transfections and infections, in fully differentiated cells and incompletely differentiated myeloid cells, and with loss of function and restoration of function approaches.
Tue Dec 21 00:00:00 EST 2021
Altered H3 histone acetylation impairs high-fidelity DNA repair to promote cerebellar degeneration in spinocerebellar ataxia type 7.
Cell reports
Switonski PM,Delaney JR,Bartelt LC,Niu C,Ramos-Zapatero M,Spann NJ,Alaghatta A,Chen T,Griffin EN,Bapat J,Sopher BL,La Spada AR
AM4300 was used in Western Blotting to conclude that altered DNA repair function in SCA7 may predispose the subject to excessive DNA damage, leading to neuron demise and highlights DNA repair as a therapy target.
Tue Nov 30 00:00:00 EST 2021
CREB regulates the expression of type 1 inositol 1,4,5-trisphosphate receptors.
Journal of cell science
Arige V,Terry LE,Malik S,Knebel TR,Wagner Ii LE,Yule DI
AM4300 was used in Western Blotting to report a crucial role for CREB in governing both the expression and correct localization of IP3R1.
Fri Oct 15 00:00:00 EDT 2021
AG-205 Upregulates Enzymes Involved in Cholesterol Biosynthesis and Steroidogenesis in Human Endometrial Cells Independently of PGRMC1 and Related MAPR Proteins.
Biomolecules
Thieffry C,Van Wynendaele M,Aynaci A,Maja M,Dupuis C,Loriot A,Marbaix E,Henriet P
AM4300 was used in Western Blotting to investigate the specificity of AG-205 as a PGRMC1 inhibitor.
Wed Oct 06 00:00:00 EDT 2021
Circ_CLASP2 Regulates High Glucose-Induced Dysfunction of Human Endothelial Cells Through Targeting miR-140-5p/FBXW7 Axis.
Frontiers in pharmacology
Zhang Q,Long J,Li N,Ma X,Zheng L
AM4300 was used in Western Blot to suggest that the overexpression of circ_CLASP2 protected HUVEC from HG-induced dysfunction at least partly through the regulation of the miR-140-5p/FBXW7 axis.
Tue Mar 30 00:00:00 EDT 2021
Effect of Microbial Status on Hepatic Odd-Chain Fatty Acids Is Diet-Dependent.
Nutrients
Weitkunat K,Bishop CA,Wittmüss M,Machate T,Schifelbein T,Schulze MB,Klaus S
AM4300 was used in Western Blot to investigate the effect of increasing microbiota complexity on hepatic lipid metabolism and odd-chain fatty acids levels in different dietary settings.
Tue May 04 00:00:00 EDT 2021
Drosophila primary microRNA-8 encodes a microRNA-encoded peptide acting in parallel of miR-8.
Genome biology
Montigny A,Tavormina P,Duboe C,San Clémente H,Aguilar M,Valenti P,Lauressergues D,Combier JP,Plaza S
AM4300 was used in Western Blot to reveal that several Drosophila pri-miRs exhibit translation potential. Contrasting with the mechanism described in plants, these data shed light on the function of yet undescribed primary-microRNA-encoded peptides in Drosophila and their regulatory potential on genome expression.
Fri Apr 23 00:00:00 EDT 2021
Alpha-Synuclein Preformed Fibrils Induce Cellular Senescence in Parkinson's Disease Models.
Cells
Verma DK,Seo BA,Ghosh A,Ma SX,Hernandez-Quijada K,Andersen JK,Ko HS,Kim YH
AM4300 was used in Western Blotting to indicate that PFF-induced pathology could lead to astrocyte and/or microglia senescence in PD brains, which may contribute to neuropathology in this model.
Mon Jul 05 00:00:00 EDT 2021
AAV9-mediated gene delivery of MCT1 to oligodendrocytes does not provide a therapeutic benefit in a mouse model of ALS.
Molecular therapy. Methods & clinical development
Eykens C,Rossaert E,Duqué S,Rué L,Bento-Abreu A,Hersmus N,Lenaerts A,Kerstens A,Corthout N,Munck S,Van Damme P,Holt MG,von Jonquires G,Klugmann M,Van Den Bosch L,Robberecht W
AM4300 was used in Western Blot to increase our current understanding about the complex nature of oligodendrocyte pathology in ALS and provides valuable insights into the future development of therapeutic strategies to efficiently modulate these cells.
Fri Mar 12 00:00:00 EST 2021
Renoprotective Effects of Maslinic Acid on Experimental Renal Fibrosis in Unilateral Ureteral Obstruction Model via Targeting MyD88.
Frontiers in pharmacology
Sun W,Byon CH,Kim DH,Choi HI,Park JS,Joo SY,Kim IJ,Jung I,Bae EH,Ma SK,Kim SW
AM4300 was used in Western Blot to clarify whether maslinic acid could attenuate renal fibrosis and determine the putative underlying molecular mechanisms.
Fri Oct 01 00:00:00 EDT 2021
The G127V variant of the prion protein interferes with dimer formation in vitro but not in cellulo.
Scientific reports
Sangeetham SB,Engelke AD,Fodor E,Krausz SL,Tatzelt J,Welker E
AM4300 was used in Western Blot to show that while molecular dynamics may capture the features affecting dimerization in vitro, G127V inhibiting dimer formation of PrP, these are not evidenced in a more complex cellular system.
Thu Feb 04 00:00:00 EST 2021
Loss of αA or αB-Crystallin Accelerates Photoreceptor Cell Death in a Mouse Model of P23H Autosomal Dominant Retinitis Pigmentosa.
International journal of molecular sciences
Wang T,Yao J,Jia L,Fort PE,Zacks DN
AM4300 was used in Western Blotting to suggest an important role for the ?-crystallins in regulating photoreceptor survival in the P23H-RHO mouse model of IRD.
Wed Dec 22 00:00:00 EST 2021
Impairment of Autophagic Flux Participates in the Antitumor Effects of TAT-Cx43266-283 in Glioblastoma Stem Cells.
Cancers
Pelaz SG,Ollauri-Ibáñez C,Lillo C,Tabernero A
AM4300 was used in Western Blotting to demonstrate that the antitumor peptide TAT-Cx43266-283, a c-Src inhibitor, blocks autophagic flux in glioblastoma stem cells (GSCs) under basal and nutrient-deprived conditions.
Tue Aug 24 00:00:00 EDT 2021
Targeting senescent cells improves functional recovery after spinal cord injury.
Cell reports
Paramos-de-Carvalho D,Martins I,Cristóvão AM,Dias AF,Neves-Silva D,Pereira T,Chapela D,Farinho A,Jacinto A,Saúde L
AM4300 was used in Western Blot to investigate the role of persistent senescent cells in the regeneration of the spinal cord in mammals.
Tue Jul 06 00:00:00 EDT 2021
Ischemic heart injury leads to HIF1-dependent differential splicing of CaMK2γ.
Scientific reports
Williams AL,Walton CB,Pinell B,Khadka VS,Dunn B,Lee K,Anagaran MCT,Avelar A,Shohet RV
AM4300 was used in Western Blotting to suggest that loss of CaMK2γ after MI is HIF1-dependent and may play an important role in the heart's calcium signaling and transcriptional response to hypoxia.
Wed Jun 23 00:00:00 EDT 2021
Tet1 Regulates Astrocyte Development and Cognition of Mice Through Modulating GluA1.
Frontiers in cell and developmental biology
Xu W,Zhang X,Liang F,Cao Y,Li Z,Qu W,Zhang J,Bi Y,Sun C,Zhang J,Sun B,Shu Q,Li X
AM4300 was used in Western Blotting to show that Tet1 deficiency alters astrocyte morphology and impairs neuronal function.
Wed Nov 17 00:00:00 EST 2021
Biochemical detection of capsid in the nucleus during HIV-1 infection.
STAR protocols
Selyutina A,Diaz-Griffero F
AM4300 was used in Western Blotting to develop a protocol to biochemically track capsid in the nucleus during infection.
Fri Mar 19 00:00:00 EDT 2021
Biochemical detection of capsid in the nucleus during HIV-1 infection.
STAR protocols
Selyutina A,Diaz-Griffero F
AM4300 was used in Western Blotting to develop a protocol to biochemically track capsid in the nucleus during infection.
Fri Mar 19 00:00:00 EDT 2021
Interspecies Differences in Proteome Turnover Kinetics Are Correlated With Life Spans and Energetic Demands.
Molecular & cellular proteomics : MCP
Swovick K,Firsanov D,Welle KA,Hryhorenko JR,Wise JP,George C,Sformo TL,Seluanov A,Gorbunova V,Ghaemmaghami S
AM4300 was used in Western Blot to suggest that in lieu of a rapid constitutive turnover, long-lived species may have evolved more energetically efficient mechanisms for selective detection and clearance of damaged proteins.
Fri Mar 11 00:00:00 EST 2022
TGF-β-dependent reprogramming of amino acid metabolism induces epithelial-mesenchymal transition in non-small cell lung cancers.
Communications biology
Nakasuka F,Tabata S,Sakamoto T,Hirayama A,Ebi H,Yamada T,Umetsu K,Ohishi M,Ueno A,Goto H,Sugimoto M,Nishioka Y,Yamada Y,Tomita M,Sasaki AT,Yano S,Soga T
AM4300 was used in Western Blot to show that TGF-β induces reprogramming of intracellular amino acid metabolism, which is necessary to promote EMT in non-small cell lung cancer cells.
Thu Jun 24 00:00:00 EDT 2021
DNA methylation is required to maintain both DNA replication timing precision and 3D genome organization integrity.
Cell reports
Du Q,Smith GC,Luu PL,Ferguson JM,Armstrong NJ,Caldon CE,Campbell EM,Nair SS,Zotenko E,Gould CM,Buckley M,Chia KM,Portman N,Lim E,Kaczorowski D,Chan CL,Barton K,Deveson IW,Smith MA,Powell JE,Skvortsova K,Stirzaker C,Achinger-Kawecka J,Clark SJ
AM4300 was used in Western Blot to highlight a potential role for the methylome in the maintenance of 3D genome regulation.
Tue Sep 21 00:00:00 EDT 2021
Pten regulates collagen fibrillogenesis by fibroblasts through SPARC.
PloS one
Jones CE,Sharick JT,Colbert SE,Shukla VC,Zent JM,Ostrowski MC,Ghadiali SN,Sizemore ST,Leight JL
AM4300 was used in Western Blot to investigate the mechanism connecting PTEN loss to increased mammary collagen deposition in breast cancer.
Wed Jul 21 00:00:00 EDT 2021
14-3-3 proteins promote synaptic localization of N-methyl d-aspartate receptors (NMDARs) in mouse hippocampal and cortical neurons.
PloS one
Lee GS,Zhang J,Wu Y,Zhou Y
AM4300 was used in Western Blotting to investigate NMDARs in mouse brains.
Tue Jan 18 00:00:00 EST 2022
MED15 prion-like domain forms a coiled-coil responsible for its amyloid conversion and propagation.
Communications biology
Batlle C,Calvo I,Iglesias V,J Lynch C,Gil-Garcia M,Serrano M,Ventura S
AM4300 was used in Western Blot to show that amyloid formation by human prion-like proteins can follow the coiled-coil-mediated model reported in yeast and that the amyloid and prion-like properties of MED15 are conserved across species.
Fri Mar 26 00:00:00 EDT 2021
TGF-β acts as a dual regulator of COX-2/PGE2 tumor promotion depending of its cross-interaction with H-Ras and Wnt/β-catenin pathways in colorectal cancer cells.
Cell biology international
de Araújo WM,Tanaka MN,Lima PHS,de Moraes CF,Leve F,Bastos LG,Rocha MR,Robbs BK,Viola JPB,Morgado-Diaz JA
AM4300 was used in Western Blotting to study the role played by TGF-β, Ras oncogene and COX-2 in colorectal cancer cell lines.
Mon Mar 01 00:00:00 EST 2021
CRISPR/Cas9-based inactivation of human papillomavirus oncogenes E6 or E7 induces senescence in cervical cancer cells.
Virology
Inturi R,Jemth P
AM4300 was used in Western Blotting to report that CRISPR/Cas9-based knockout of E6 or E7 can trigger cellular senescence in HPV18 immortalized HeLa cells.
Fri Oct 01 00:00:00 EDT 2021
Bisphenol A promotes stress granule assembly and modulates the integrated stress response.
Biology open
Fay MM,Columbo D,Cotter C,Friend C,Henry S,Hoppe M,Karabelas P,Lamy C,Lawell M,Monteith S,Noyes C,Salerno P,Wu J,Zhang HM,Anderson PJ,Kedersha N,Ivanov P,Farny NG
AM4300 was used in Western Blotting to demonstrate that high-dose BPA causes stress granules (SGs) in human cells.
Sun Jan 10 00:00:00 EST 2021
Proteotoxic Stress as an Exploitable Vulnerability in Cells with Hyperactive AKT.
International journal of molecular sciences
Babagana M,Brown LR,Slabodkin HZ,Kichina JV,Kandel ES
AM4300 was used in Western Blot to suggest that proteotoxic stress conferred by hyperactive AKT represents a targetable vulnerability, which can be exploited by either elevating the stress above the level tolerated by such cells or by eliminating the factors that enable such tolerance.
Thu Oct 21 00:00:00 EDT 2021
Tryptophan Metabolism Regulates Proliferative Capacity of Human Pluripotent Stem Cells.
iScience
Someya S,Tohyama S,Kameda K,Tanosaki S,Morita Y,Sasaki K,Kang MI,Kishino Y,Okada M,Tani H,Soma Y,Nakajima K,Umei T,Sekine O,Moriwaki T,Kanazawa H,Kobayashi E,Fujita J,Fukuda K
AM4300 was used in Western Blot to demonstrate that TRP is indispensable for survival and proliferation of hPSCs. A deeper understanding of TRP metabolism will enable cost-effective large-scale production of hPSCs, leading to advances in regenerative medicine.
Fri Feb 19 00:00:00 EST 2021
MCT1 Deletion in Oligodendrocyte Lineage Cells Causes Late-Onset Hypomyelination and Axonal Degeneration.
Cell reports
Philips T,Mironova YA,Jouroukhin Y,Chew J,Vidensky S,Farah MH,Pletnikov MV,Bergles DE,Morrison BM,Rothstein JD
AM4300 was used in Western Blot to show that loss of OL lineage MCT1 expression with aging leads to significant axonal degeneration with concomitant hypomyelination.
Tue Jan 12 00:00:00 EST 2021
TNF-α synergises with IFN-γ to induce caspase-8-JAK1/2-STAT1-dependent death of intestinal epithelial cells.
Cell death & disease
Woznicki JA,Saini N,Flood P,Rajaram S,Lee CM,Stamou P,Skowyra A,Bustamante-Garrido M,Regazzoni K,Crawford N,McDade SS,Longley DB,Aza-Blanc P,Shanahan F,Zulquernain SA,McCarthy J,Melgar S,McRae BL,Nally K
AM4300 was used in Western Blot to identify JAK1/2 kinases as the principal and nonredundant drivers of the synergistic killing of human IECs by IFN-γ/TNF-α.
Thu Sep 23 00:00:00 EDT 2021
Mitochondria-rough-ER contacts in the liver regulate systemic lipid homeostasis.
Cell reports
Anastasia I,Ilacqua N,Raimondi A,Lemieux P,Ghandehari-Alavijeh R,Faure G,Mekhedov SL,Williams KJ,Caicci F,Valle G,Giacomello M,Quiroga AD,Lehner R,Miksis MJ,Toth K,de Aguiar Vallim TQ,Koonin EV,Scorrano L,Pellegrini L
AM4300 was used in Western Blot to study mitochondria-rER contacts in vivo to elucidate their ultrastructural organization, dynamics, and functions.
Tue Mar 16 00:00:00 EDT 2021
The fragile X mental retardation protein promotes adjustments in cocaine self-administration that preserve reinforcement level.
The European journal of neuroscience
Huebschman JL,Davis MC,Tovar Pensa C,Guo Y,Smith LN
AM4300 was used in Western Blotting to suggest that FMRP facilitates adjustments in drug self-administration behavior that generally serve to preserve reinforcement level, and combined with our similar IVSA findings in Arc KO mice may implicate Arc, along with FMRP, in behavioral shifts that occur in drug taking when drug availability is altered.
Sun Aug 01 00:00:00 EDT 2021
Novel Compound Heterozygous Pathogenic Mutations of SLC5A5 in a Chinese Patient With Congenital Hypothyroidism.
Frontiers in endocrinology
Zhang CX,Zhang JX,Yang L,Zhang CR,Cheng F,Zhang RJ,Fang Y,Wang Z,Wu FY,Li PZ,Liang J,Li R,Song HD
AM4300 was used in Western Blotting to investigate the expression and cellular localization of the novel compound heterozygous mutation in SLC5A5. The functional activity of the mutants was further examined in vitro.
Fri Dec 10 00:00:00 EST 2021
The Effects of Maternal Smoking on Pregnancy and Offspring: Possible Role for EGF?
Frontiers in cell and developmental biology
Janbazacyabar H,van Daal M,Leusink-Muis T,van Ark I,Garssen J,Folkerts G,van Bergenhenegouwen J,Braber S
AM4300 was used in Western Blotting to reveal that maternal smoke exposure predisposes dams to exacerbated airway inflammation and offspring to exacerbated immune responses and both phenomena are associated with elevated EGF concentrations.
Tue Sep 07 00:00:00 EDT 2021
Pericentromeric Satellite III transcripts induce etoposide resistance.
Cell death & disease
Kanne J,Hussong M,Isensee J,Muñoz-López Á,Wolffgramm J,Heß F,Grimm C,Bessonov S,Meder L,Wang J,Reinhardt HC,Odenthal M,Hucho T,Büttner R,Summerer D,Schweiger MR
AM4300 was used in Western Blotting to e show that the long non-coding satellite III RNA (SatIII) generates resistance against the topoisomerase IIa (TOP2A) inhibitor etoposide in lung cancer.
Mon May 24 00:00:00 EDT 2021
Loss of Tsc1 in cerebellar Purkinje cells induces transcriptional and translation changes in FMRP target transcripts.
eLife
Dalal JS,Winden KD,Salussolia CL,Sundberg M,Singh A,Pham TT,Zhou P,Pu WT,Miller MT,Sahin M
AM4300 was used in Western Blot to implicate dysfunction of FMRP and its targets in TSC and suggest that treatments aimed at restoring the function of these pathways may be beneficial.
Wed Jul 14 00:00:00 EDT 2021
Loss of presenilin function enhances tau phosphorylation and aggregation in mice.
Acta neuropathologica communications
Soto-Faguás CM,Sanchez-Molina P,Saura CA
AM4300 was used in Immunohistochemistry to report that neuronal presenilin deficiency in conditional knockout (cKO) mice results in age-dependent brain atrophy, inflammatory responses and accumulation of pathological tau in neurons and glial cells.
Thu Sep 30 00:00:00 EDT 2021
STING regulates peripheral nerve regeneration and colony stimulating factor 1 receptor (CSF1R) processing in microglia.
iScience
Morozzi G,Rothen J,Toussaint G,De Lange K,Westritschnig K,Doelemeyer A,Ueberschlag VP,Kahle P,Lambert C,Obrecht M,Beckmann N,Ritter V,Panesar M,Stauffer D,Garnier I,Mueller M,Guerini D,Keller CG,Knehr J,Roma G,Bidinosti M,Brachat S,Morvan F,Fornaro M
AM4300 was used in Western Blot to investigate relationship between peripheral nerve injury and inflammation.
Fri Dec 17 00:00:00 EST 2021
MATR3 haploinsufficiency and early-onset neurodegeneration.
Brain : a journal of neurology
Zech M,Seibt A,Zumbaum B,Klee D,Meitinger T,Winkelmann J,Mayatepek E,Wagner M,Distelmaier F
AM4300 was used in Western Blotting to discuss MATR3 haploinsufficiency and early-onset neurodegeneration.
Fri Oct 22 00:00:00 EDT 2021
A conserved cysteine-based redox mechanism sustains TFEB/HLH-30 activity under persistent stress.
The EMBO journal
Martina JA,Guerrero-Gómez D,Gómez-Orte E,Antonio Bárcena J,Cabello J,Miranda-Vizuete A,Puertollano R
AM4300 was used in Western Blotting to study a novel mechanism of TFEB/HLH-30 regulation through a cysteine-mediated redox switch.
Mon Feb 01 00:00:00 EST 2021
Alterations in the Gut-Microbial-Inflammasome-Brain Axis in a Mouse Model of Alzheimer's Disease.
Cells
Shukla PK,Delotterie DF,Xiao J,Pierre JF,Rao R,McDonald MP,Khan MM
AM4300 was used in Western Blotting to provide a potential mechanistic link between dysbiotic gut microbiota and neuroinflammation associated with AD progression.
Thu Apr 01 00:00:00 EDT 2021
CRISPR screens in cancer spheroids identify 3D growth-specific vulnerabilities.
Nature
Han K,Pierce SE,Li A,Spees K,Anderson GR,Seoane JA,Lo YH,Dubreuil M,Olivas M,Kamber RA,Wainberg M,Kostyrko K,Kelly MR,Yousefi M,Simpkins SW,Yao D,Lee K,Kuo CJ,Jackson PK,Sweet-Cordero A,Kundaje A,Gentles AJ,Curtis C,Winslow MM,Bassik MC
AM4300 was used in Western Blotting to reveal key differences between 2D and 3D cancer models, and establish a generalizable strategy for performing CRISPR screens in spheroids to reveal cancer vulnerabilities.
Wed Apr 01 00:00:00 EDT 2020
Splicing Kinetics and Coordination Revealed by Direct Nascent RNA Sequencing through Nanopores.
Molecular cell
Drexler HL,Choquet K,Churchman LS
AM4300 was used in Western Blotting to conclude that nano-COP unveils the organizational complexity of RNA processing.
Thu Mar 05 00:00:00 EST 2020
G4C2 Repeat RNA Initiates a POM121-Mediated Reduction in Specific Nucleoporins in C9orf72 ALS/FTD.
Neuron
Coyne AN,Zaepfel BL,Hayes L,Fitchman B,Salzberg Y,Luo EC,Bowen K,Trost H,Aigner S,Rigo F,Yeo GW,Harel A,Svendsen CN,Sareen D,Rothstein JD
AM4300 was used in Western Blot to suggest that the expression of expanded C9orf72 ALS/FTD repeat RNA alone affects nuclear POM121 expression in the initiation of a pathological cascade affecting nucleoporin levels within neuronal nuclei and ultimately downstream neuronal survival.
Wed Sep 23 00:00:00 EDT 2020
Talin1 controls dendritic cell activation by regulating TLR complex assembly and signaling.
The Journal of experimental medicine
Lim TJF,Bunjamin M,Ruedl C,Su IH
AM4300 was used in Western Blotting to investigate the role and mechanism of Talin in regulating skin dendritic cells (DCs) during inflammatory responses.
Mon Aug 03 00:00:00 EDT 2020
Additive Therapeutic Effects of Mesenchymal Stem Cells and IL-37 for Systemic Lupus Erythematosus.
Journal of the American Society of Nephrology : JASN
Xu J,Chen J,Li W,Lian W,Huang J,Lai B,Li L,Huang Z
AM4300 was used in Western Blotting to investigate the effect of overexpressing IL-37 on the therapeutic effects of mesenchymal stem cells for Systemic Lupus Erythematosus treatment.
Wed Jan 01 00:00:00 EST 2020
Mechanism and inhibition of the papain-like protease, PLpro, of SARS-CoV-2.
The EMBO journal
Klemm T,Ebert G,Calleja DJ,Allison CC,Richardson LW,Bernardini JP,Lu BG,Kuchel NW,Grohmann C,Shibata Y,Gan ZY,Cooney JP,Doerflinger M,Au AE,Blackmore TR,van der Heden van Noort GJ,Geurink PP,Ovaa H,Newman J,Riboldi-Tunnicliffe A,Czabotar PE,Mitchell JP,Feltham R,Lechtenberg BC,Lowes KN,Dewson G,Pellegrini M,Lessene G,Komander D
AM4300 was used in Western Blot to identify papain-like protease domain inhibitors for potential antiviral activity in SARS-CoV-2 infection.
Tue Sep 15 00:00:00 EDT 2020
Astrocytic phagocytosis is a compensatory mechanism for microglial dysfunction.
The EMBO journal
Konishi H,Okamoto T,Hara Y,Komine O,Tamada H,Maeda M,Osako F,Kobayashi M,Nishiyama A,Kataoka Y,Takai T,Udagawa N,Jung S,Ozato K,Tamura T,Tsuda M,Yamanaka K,Ogi T,Sato K,Kiyama H
AM4300 was used in Western Blotting to demonstrate that astrocytes, rather than nonmicroglial mononuclear cells, readily phagocytose microglial debris in our microglia ablation model.
Mon Nov 16 00:00:00 EST 2020
USP7 regulates ALS-associated proteotoxicity and quality control through the NEDD4L-SMAD pathway.
Proceedings of the National Academy of Sciences of the United States of America
Zhang T,Periz G,Lu YN,Wang J
AM4300 was used in Western Blotting to report the identification of a ubiquitin-specific protease, USP7, as a regulatory switch in a protein quality-control system that defends against proteotoxicity.
Tue Nov 10 00:00:00 EST 2020
Bi-allelic Variants in RALGAPA1 Cause Profound Neurodevelopmental Disability, Muscular Hypotonia, Infantile Spasms, and Feeding Abnormalities.
American journal of human genetics
Wagner M,Skorobogatko Y,Pode-Shakked B,Powell CM,Alhaddad B,Seibt A,Barel O,Heimer G,Hoffmann C,Demmer LA,Perilla-Young Y,Remke M,Wieczorek D,Navaratnarajah T,Lichtner P,Klee D,Shamseldin HE,Al Mutairi F,Mayatepek E,Strom T,Meitinger T,Alkuraya FS,Anikster Y,Saltiel AR,Distelmaier F
AM4300 was used in Western Blotting to indicate that the dysregulation of the RalA pathway has an important impact on neuronal function and brain development.
Thu Feb 06 00:00:00 EST 2020
Age-induced mitochondrial DNA point mutations are inadequate to alter metabolic homeostasis in response to nutrient challenge.
Aging cell
Moore TM,Zhou Z,Strumwasser AR,Cohn W,Lin AJ,Cory K,Whitney K,Ho T,Ho T,Lee JL,Rucker DH,Hoang AN,Widjaja K,Abrishami AD,Charugundla S,Stiles L,Whitelegge JP,Turcotte LP,Wanagat J,Hevener AL
AM4300 was used in Western Blotting to investigate the dynamics and quality control signaling in mouse muscle and liver to determine whether mitochondria respond to mtDNA point mutations by altering morphology and turnover.
Sun Nov 01 00:00:00 EDT 2020
MicroRNA-19a-PTEN Axis Is Involved in the Developmental Decline of Axon Regenerative Capacity in Retinal Ganglion Cells.
Molecular therapy. Nucleic acids
Mak HK,Yung JSY,Weinreb RN,Ng SH,Cao X,Ho TYC,Ng TK,Chu WK,Yung WH,Choy KW,Wang CC,Lee TL,Leung CK
AM4300 was used in Western Blotting to uncover a previously unrecognised involvement of the miR-19a-PTEN axis in RGC axon regeneration, and it demonstrates therapeutic potential of microRNA-mediated restoration of axon regenerative capacity in optic neuropathies.
Fri Sep 04 00:00:00 EDT 2020
Enteroids Derived From Inflammatory Bowel Disease Patients Display Dysregulated Endoplasmic Reticulum Stress Pathways, Leading to Differential Inflammatory Responses and Dendritic Cell Maturation.
Journal of Crohn's & colitis
Rees WD,Stahl M,Jacobson K,Bressler B,Sly LM,Vallance BA,Steiner TS
AM4300 was used in Western Blot to test a hypothesis that ER stress changes innate signalling in human IECs, augmenting toll-like receptor [TLR] responses and inducing pro-inflammatory changes in underlying dendritic cells [DCs].
Thu Jul 30 00:00:00 EDT 2020
Sporadic activation of an oxidative stress-dependent NRF2-p53 signaling network in breast epithelial spheroids and premalignancies.
Science signaling
Pereira EJ,Burns JS,Lee CY,Marohl T,Calderon D,Wang L,Atkins KA,Wang CC,Janes KA
AM4300 was used in Western Blotting to findd that cellular states was jointly coordinated by the NRF2 and p53 pathways, which are costabilized by spontaneous oxidative stress within 3D cultures.
Tue Apr 14 00:00:00 EDT 2020
Quantitative analysis of Y-Chromosome gene expression across 36 human tissues.
Genome research
Godfrey AK,Naqvi S,Chmátal L,Chick JM,Mitchell RN,Gygi SP,Skaletsky H,Page DC
AM4300 was used in Western Blotting to analyze quantitative profiles of Y-Chromosome gene expression across 36 human tissues from hundreds of individuals.
Mon Jun 01 00:00:00 EDT 2020
Recombinant human ADAMTS13 treatment and anti-NET strategies enhance skin allograft survival in mice.
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons
Wong SL,Goverman J,Staudinger C,Wagner DD
AM4300 was used in Western Blotting to explore the effect of ADAMTS13 treatment and the impact of NETs on skin allografts.
Wed Apr 01 00:00:00 EDT 2020
Context-dependent activation of SIRT3 is necessary for anchorage-independent survival and metastasis of ovarian cancer cells.
Oncogene
Kim YS,Gupta Vallur P,Jones VM,Worley BL,Shimko S,Shin DH,Crawford LC,Chen CW,Aird KM,Abraham T,Shepherd TG,Warrick JI,Lee NY,Phaeton R,Mythreye K,Hempel N
AM4300 was used in Western Blotting to highlight the novel context-specific, pro-metastatic role of SIRT3 in ovarian cancer.
Sat Feb 01 00:00:00 EST 2020
Nek2-mediated GAS2L1 phosphorylation and centrosome-linker disassembly induce centrosome disjunction.
The Journal of cell biology
Au FKC,Hau BKT,Qi RZ
AM4300 was used in Immunocytochemistry to show that GAS2L1, a microtubule- and F-actin-binding protein required for centrosome disjunction, undergoes Nek2-mediated phosphorylation at Ser352 in G2/M.
Mon May 04 00:00:00 EDT 2020
Septin2 mediates podosome maturation and endothelial cell invasion associated with angiogenesis.
The Journal of cell biology
Collins KB,Kang H,Matsche J,Klomp JE,Rehman J,Malik AB,Karginov AV
AM4300 was used in Western Blot to inestigate if targeting of Septin2-mediated podosome formation is a potentially attractive anti-angiogenesis strategy.
Mon Feb 03 00:00:00 EST 2020
Ste20-like Kinase-mediated Control of Actin Polymerization Is a New Mechanism for Thin Filament-associated Regulation of Airway Smooth Muscle Contraction.
American journal of respiratory cell and molecular biology
Wang Y,Wang R,Tang DD
AM4300 was used in Western Blotting to suggest that SLK regulates paxillin phosphorylation at Ser-272 via Plk1, which modulates N-WASP activation and actin polymerization in smooth muscle. SLK-mediated actin cytoskeletal reorganization may facilitate force transmission between the contractile units and the extracellular matrix.
Fri May 01 00:00:00 EDT 2020
Glioblastoma initiating cells are sensitive to histone demethylase inhibition due to epigenetic deregulation.
International journal of cancer
Mallm JP,Windisch P,Biran A,Gal Z,Schumacher S,Glass R,Herold-Mende C,Meshorer E,Barbus M,Rippe K
AM4300 was used in Western Blot to identify stem cell-like chromatin features in human glioblastoma initiating cells (GICs) and link them to a loss of the repressive histone H3 lysine 9 trimethylation (H3K9me3) mark.
Sun Mar 01 00:00:00 EST 2020
Autophagy Upregulation by the TFEB Inducer Trehalose Protects against Oxidative Damage and Cell Death Associated with NRF2 Inhibition in Human RPE Cells.
Oxidative medicine and cellular longevity
Abokyi S,Shan SW,To CH,Chan HH,Tse DY
AM4300 was used in Western Blotting to support the transcriptional upregulation of TFEB and autophagy by trehalose and its protection against HQ-induced oxidative damage in RPE cells.
Fri May 14 00:00:00 EDT 2021
Impairment of adenosinergic system in Rett syndrome: Novel therapeutic target to boost BDNF signalling.
Neurobiology of disease
Miranda-Lourenço C,Duarte ST,Palminha C,Gaspar C,Rodrigues TM,Magalhães-Cardoso T,Rei N,Colino-Oliveira M,Gomes R,Ferreira S,Rosa J,Xapelli S,Armstrong J,García-Cazorla À,Correia-de-Sá P,Sebastião AM,Diógenes MJ
AM4300 was used in Western Blotting to characterize several hallmarks of the adenosinergic and BDNF signalling in RTT and explore whether A2AR activation could boost BDNF actions.
Sun Nov 01 00:00:00 EDT 2020
Ykt6-dependent endosomal recycling is required for Wnt secretion in the Drosophila wing epithelium.
Development (Cambridge, England)
Linnemannstöns K,Witte L,Karuna M P,Kittel JC,Danieli A,Müller D,Nitsch L,Honemann-Capito M,Grawe F,Wodarz A,Gross JC
AM4300 was used in Western Blotting to investigate how Wnts are sorted at the level of endosomes by the versatile v-SNARE Ykt6.
Fri Aug 14 00:00:00 EDT 2020
Loss of TREM2 Confers Resilience to Synaptic and Cognitive Impairment in Aged Mice.
The Journal of neuroscience : the official journal of the Society for Neuroscience
Qu W,Li L
AM4300 was used in Western Blot to unveil the positive impacts of TREM2 deficiency on cognitive and synaptic function during aging and suggest that TREM2 may exert detrimental effects on neuronal function.
Wed Dec 09 00:00:00 EST 2020
Antiviral Properties of the LSD1 Inhibitor SP-2509.
Journal of virology
Harancher MR,Packard JE,Cowan SP,DeLuca NA,Dembowski JA
AM4300 was used in Western Blotting to support a potential new role for LSD1 in the regulation of viral DNA replication and successive steps in the virus life cycle, and further highlight the promising potential to utilize LSD1 inhibition as an antiviral approach.
Tue Sep 15 00:00:00 EDT 2020
Characterization of 7A5: A Human CD137 (4-1BB) Receptor Binding Monoclonal Antibody with Differential Agonist Properties That Promotes Antitumor Immunity.
Molecular cancer therapeutics
Kotanides H,Sattler RM,Lebron MB,Carpenito C,Shen J,Li J,Surguladze D,Haidar JN,Burns C,Shen L,Inigo I,Pennello AL,Forest A,Chen X,Chin D,Sonyi A,Topper M,Boucher L,Sharma P,Zhang Y,Burtrum D,Novosiadly RD,Ludwig DL,Plowman GD,Kalos M
AM4300 was used in Western Blotting to demonstrate 7A5 is a differentiated CD137 agonist antibody with biological properties that warrant its further development as a cancer immunotherapy.
Wed Apr 01 00:00:00 EDT 2020
The branched-chain amino acids valine and leucine have differential effects on hepatic lipid metabolism.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
Bishop CA,Schulze MB,Klaus S,Weitkunat K
AM4300 was used in Western Blotting to show the differential roles of branched-chain amino acids in lipid metabolism and endogenous formation of circulating odd-chain fatty acids is exclusively mediated by valine.
Wed Jul 01 00:00:00 EDT 2020
Transcriptome signature of ventricular arrhythmia in dilated cardiomyopathy reveals increased fibrosis and activated TP53.
Journal of molecular and cellular cardiology
Haywood ME,Cocciolo A,Porter KF,Dobrinskikh E,Slavov D,Graw SL,Reece TB,Ambardekar AV,Bristow MR,Mestroni L,Taylor MRG
AM4300 was used in Western Blotting to identify transcriptome signatures and enriched pathways in the hearts of dilated cardiomyopathy (DCM) patients with ventricular tachycardia (VT).
Sat Feb 01 00:00:00 EST 2020
An RNA guanine quadruplex regulated pathway to TRAIL-sensitization by DDX21.
RNA (New York, N.Y.)
McRae EKS,Dupas SJ,Booy EP,Piragasam RS,Fahlman RP,McKenna SA
AM4300 was used in Western Blotting to identify the role of DDX21 in regulation at the translational level through biologically relevant rG4 and show that MAGED2 protein levels are regulated, at least in part, by the potential to form rG4 in their 5'-UTRs.
Wed Jan 01 00:00:00 EST 2020
Neuropilin-1 is up-regulated by cancer-associated fibroblast-secreted IL-8 and associated with cell proliferation of gallbladder cancer.
Journal of cellular and molecular medicine
Chen C,Zhang R,Ma L,Li Q,Zhao YL,Zhang GJ,Zhang D,Li WZ,Cao S,Wang L,Geng ZM
AM4300 was used in Western Blotting to demonstrate how Neuropilin-1 causes gallbladder cancer cell proliferation through upregulation by cancer-associated fibroblast-secreted IL-8.
Sun Nov 01 00:00:00 EDT 2020
Abl-mediated PI3K activation regulates macrophage podosome formation.
Journal of cell science
Zhou Y,Feng Z,Cao F,Liu X,Xia X,Yu CH
AM4300 was used in Western Blotting to show that phosphatidylinositol (3,4,5)-trisphosphate [PI(3,4,5)P3] lipids are enriched at the podosome and stably recruit WASP rather than the WASP-5KE mutant.
Mon Jun 08 00:00:00 EDT 2020
Osmotic gradients induce stable dome morphogenesis on extracellular matrix.
Journal of cell science
Ishida-Ishihara S,Akiyama M,Furusawa K,Naguro I,Ryuno H,Sushida T,Ishihara S,Haga H
AM4300 was used in Western Blotting to indicate that osmotic gradients induce dome morphogenesis via both enhanced water transport activity and subsequent ECM swelling.
Mon Jul 27 00:00:00 EDT 2020
The role of polo-like kinase 3 in the response of BRAF-mutant cells to targeted anticancer therapies.
Molecular carcinogenesis
Babagana M,Kichina JV,Slabodkin H,Johnson S,Maslov A,Brown L,Attwood K,Nikiforov MA,Kandel ES
AM4300 was used in Western Blotting to study the underlying resistance mechanisms of BRAF inhibitors.
Wed Jan 01 00:00:00 EST 2020
Bim contributes to the progression of Huntington's disease-associated phenotypes.
Human molecular genetics
Roberts SL,Evans T,Yang Y,Fu Y,Button RW,Sipthorpe RJ,Cowan K,Valionyte E,Anichtchik O,Li H,Lu B,Luo S
AM4300 was used in Western Blot to investigate if Bim could contribute to the progression of Huntington's disease.
Wed Jan 15 00:00:00 EST 2020
AKT signaling promotes DNA damage accumulation and proliferation in polycystic kidney disease.
Human molecular genetics
Conduit SE,Davies EM,Ooms LM,Gurung R,McGrath MJ,Hakim S,Cottle DL,Smyth IM,Dyson JM,Mitchell CA
AM4300 was used in Western Blot to demonstrate AKT and mTORC1 hyperactivation in two representative murine PKD models (renal epithelial-specific Inpp5e knockout and collecting duct-specific Pkd1 deletion) and identify a downstream signaling network that contributes to DNA damage accumulation.
Wed Jan 01 00:00:00 EST 2020
Loss of High-Mobility Group Box 1 (HMGB1) Protein in Rods Accelerates Rod Photoreceptor Degeneration After Retinal Detachment.
Investigative ophthalmology & visual science
Ross BX,Choi J,Yao J,Hager HM,Abcouwer SF,Zacks DN
AM4300 was used in Western Blotting to investigate the relationship between HMGB1 and PR survival after RD.
Mon May 11 00:00:00 EDT 2020
A Highly Selective Chemical Probe for Activin Receptor-like Kinases ALK4 and ALK5.
ACS chemical biology
Hanke T,Wong JF,Berger BT,Abdi I,Berger LM,Tesch R,Tredup C,Bullock AN,Müller S,Knapp S
AM4300 was used in Western Blot to report the characterisation of the 2-oxo-imidazopyridine, TP-008, a potent chemical probe with dual activity for ALK4 and ALK5 as well as the development of a matching negative control compound.
Fri Apr 17 00:00:00 EDT 2020
miR-125 family regulates XIRP1 and FIH in response to myocardial infarction.
Physiological genomics
Williams AL,Khadka VS,Anagaran MCT,Lee K,Avelar A,Deng Y,Shohet RV
AM4300 was used in Western Blot to characterize miRNA expression in the mouse heart after coronary artery ligation and identify novel mRNA targets altered during the initial response to ischemic stress.
Sat Aug 01 00:00:00 EDT 2020
Prostatic osteopontin expression is associated with symptomatic benign prostatic hyperplasia.
The Prostate
Popovics P,Awadallah WN,Kohrt SE,Case TC,Miller NL,Ricke EA,Huang W,Ramirez-Solano M,Liu Q,Vezina CM,Matusik RJ,Ricke WA,Grabowska MM
AM4300 was used in Western Blotting to test whether prostate epithelial and stromal cells secrete OPN in response to proinflammatory stimuli and identified downstream targets of OPN in prostate stromal cells.
Wed Jul 01 00:00:00 EDT 2020
Increased nuclear factor I/B expression in prostate cancer correlates with AR expression.
The Prostate
Nanda JS,Awadallah WN,Kohrt SE,Popovics P,Cates JMM,Mirosevich J,Clark PE,Giannico GA,Grabowska MM
AM4300 was used in Western Blot to describe the expression pattern of nuclear factor I/B in primary and castration-resistant prostate cancer and its correlation with androgen receptor.
Tue Sep 01 00:00:00 EDT 2020
Inhibitory Activity of Boesenbergia rotunda (L.) Mansf. Rhizome towards the Expression of Akt and NF-KappaB p65 in Acetic Acid-Induced Wistar Rats.
Evidence-based complementary and alternative medicine : eCAM
Rosdianto AM,Puspitasari IM,Lesmana R,Levita J
AM4300 was used in Western Blot to conclude that BREE could inhibit the expression of Akt and NF-kappaB p65 in the stomach and intestine of acetic acid-induced Wistar rats.
Fri Apr 15 00:00:00 EDT 2022
Characterization of MORN2 stability and regulatory function in LC3-associated phagocytosis in macrophages.
Biology open
Morita M,Kajiye M,Sakurai C,Kubo S,Takahashi M,Kinoshita D,Hori N,Hatsuzawa K
AM4300 was used in Western Blotting to demonstrate that MORN2, whose expression is downregulated via proteasomal digestion, is a limiting factor for LAP, and that membrane trafficking by SNARE proteins is involved in MORN2-mediated LAP.
Tue Jun 23 00:00:00 EDT 2020
Human THO maintains the stability of repetitive DNA.
Genes to cells : devoted to molecular & cellular mechanisms
Katahira J,Senokuchi K,Hieda M
AM4300 was used in Western Blotting to analyse the genome-wide distribution of human THOC7, a component of human THO, by chromatin immunoprecipitation sequencing.
Fri May 01 00:00:00 EDT 2020
Novel calreticulin-nanoparticle in combination with focused ultrasound induces immunogenic cell death in melanoma to enhance antitumor immunity.
Theranostics
Sethuraman SN,Singh MP,Patil G,Li S,Fiering S,Hoopes PJ,Guha C,Malayer J,Ranjan A
AM4300 was used in Western Blotting to report a novel calreticulin-nanoparticle that enhances ICD and synergizes with focused ultrasound to achieve local and systemic antitumor effects.
Tue Apr 27 00:00:00 EDT 2021
Different training intensities induced autophagy and histopathology appearances potentially associated with lipid metabolism in wistar rat liver.
Heliyon
Gunadi JW,Tarawan VM,Daniel Ray HR,Wahyudianingsih R,Lucretia T,Tanuwijaya F,Lesmana R,Supratman U,Setiawan I
AM4300 was used in Western Blotting to investigate the potential linking role of autophagy-related gene expressions and protein levels with histopathology changes in Wistar rat livers after treadmill training under different intensities.
Fri May 01 00:00:00 EDT 2020
Human GBP1 binds LPS to initiate assembly of a caspase-4 activating platform on cytosolic bacteria.
Nature communications
Santos JC,Boucher D,Schneider LK,Demarco B,Dilucca M,Shkarina K,Heilig R,Chen KW,Lim RYH,Broz P
AM4300 was used in Western Blotting to demonstrate that human GBP1 acts as a cytosolic LPS sensor, assembling a platform that promotes caspase-4 recruitment and activation, the first step in non-canonical inflammasome signaling.
Wed Jun 24 00:00:00 EDT 2020
Functional interrogation of HOXA9 regulome in MLLr leukemia via reporter-based CRISPR/Cas9 screen.
eLife
Zhang H,Zhang Y,Zhou X,Wright S,Hyle J,Zhao L,An J,Zhao X,Shao Y,Xu B,Lee HM,Chen T,Zhou Y,Chen X,Lu R,Li C
AM4300 was used in Western Blotting to investigate HOXA9 regulation using cell lines.
Thu Oct 01 00:00:00 EDT 2020
Targeting metabolic plasticity in glioma stem cells in vitro and in vivo through specific inhibition of c-Src by TAT-Cx43266-283.
EBioMedicine
Pelaz SG,Jaraíz-Rodríguez M,Álvarez-Vázquez A,Talaverón R,García-Vicente L,Flores-Hernández R,Gómez de Cedrón M,Tabernero M,Ramírez de Molina A,Lillo C,Medina JM,Tabernero A
AM4300 was used in Western Blotting to explore the inhibition of GSCs to investigate its possible use in treatments.
Tue Dec 01 00:00:00 EST 2020
Wnt/β-catenin-activated Ewing sarcoma cells promote the angiogenic switch.
JCI insight
Hawkins AG,Pedersen EA,Treichel S,Temprine K,Sperring C,Read JA,Magnuson B,Chugh R,Lawlor ER
AM4300 was used in Western Blotting to suggest that Wnt/β-catenin-active tumor cells can contribute to Ewing sarcoma progression by promoting angiogenesis in the local TME.
Thu Jul 09 00:00:00 EDT 2020
Low Doses of Methylmercury Induce the Proliferation of Thyroid Cells In Vitro Through Modulation of ERK Pathway.
International journal of molecular sciences
Maggisano V,Bulotta S,Celano M,Maiuolo J,Lepore SM,Abballe L,Iannone M,Russo D
AM4300 was used in Western Blot to analyze the effects of methylmercury (MeHg) on immortalized, non-tumorigenic ‘normal’ thyroid cells Nthy-ori-3-1 and the molecular changes occurring after exposure to MeHg.
Tue Feb 25 00:00:00 EST 2020
The GSK-3β-FBXL21 Axis Contributes to Circadian TCAP Degradation and Skeletal Muscle Function.
Cell reports
Wirianto M,Yang J,Kim E,Gao S,Paudel KR,Choi JM,Choe J,Gloston GF,Ademoji P,Parakramaweera R,Jin J,Esser KA,Jung SY,Geng YJ,Lee HK,Chen Z,Yoo SH
AM4300 was used in Western Blot to highlight a circadian regulatory pathway where a GSK-3β-FBXL21 functional axis controls TCAP degradation via SCF complex formation and regulates skeletal muscle function.
Tue Sep 15 00:00:00 EDT 2020
A loop involving NRF2, miR-29b-1-5p and AKT, regulates cell fate of MDA-MB-231 triple-negative breast cancer cells.
Journal of cellular physiology
De Blasio A,Di Fiore R,Pratelli G,Drago-Ferrante R,Saliba C,Baldacchino S,Grech G,Scerri C,Vento R,Tesoriere G
AM4300 was used in Western Blotting to uncover a novel NRF2/miR-29b-1-5p/AKT regulatory loop that can regulate the fate (life/death) of MDA-MB-231 cells and suggests this loop as therapeutic target for TNBC.
Sat Feb 01 00:00:00 EST 2020
Potential synergistic effects of sorafenib and CP-31398 for treating anaplastic thyroid cancer with p53 mutations.
Oncology letters
Wu JT,Lin CL,Huang CJ,Cheng YC,Chien CC,Sung YC
AM4300 was used in Western Blotting to indicate a potential clinical application of CP-31398 for patients with ATC harboring p53 abnormalities, since these individuals generally respond poorly to sorafenib alone.
Wed Apr 01 00:00:00 EDT 2020
Proteasomal-Mediated Degradation of AKAP150 Accompanies AMPAR Endocytosis during cLTD.
eNeuro
Cheng W,Siedlecki-Wullich D,Català-Solsona J,Fábregas C,Fadó R,Casals N,Solé M,Unzeta M,Saura CA,Rodríguez-Alvarez J,Miñano-Molina AJ
AM4300 was used in Western Blotting to show synaptic AMPAR endocytosis is influenced by proteasome degradation of A-kinase anchoring protein AKAP150.
Fri Jun 18 00:00:00 EDT 2021
Phosphorylation of Ykt6 SNARE Domain Regulates Its Membrane Recruitment and Activity.
Biomolecules
Karuna M P,Witte L,Linnemannstoens K,Choezom D,Danieli-Mackay A,Honemann-Capito M,Gross JC
AM4300 was used in Western Blotting to show phosphorylation of SNARE protein Ykt6 leads induces a conformational change from an auto-inhibited state to an active state.
Mon Nov 16 00:00:00 EST 2020
Mouse Ifit1b is a cap1-RNA-binding protein that inhibits mouse coronavirus translation and is regulated by complexing with Ifit1c.
The Journal of biological chemistry
Mears HV,Sweeney TR
AM4300 was used in Western Blotting to find that Ifit1b preferentially binds to cap1-RNA, whereas binding is much weaker to cap0- and cap2-RNA.
Fri Dec 18 00:00:00 EST 2020
A transcriptional roadmap for 2C-like-to-pluripotent state transition.
Science advances
Fu X,Djekidel MN,Zhang Y
AM4300 was used in Western Blotting to analyze the transcriptional dynamics from the 2C-like state to pluripotent ESCs and identified an intermediate state.
Fri May 01 00:00:00 EDT 2020
Chicken avian β-defensin 8 modulates immune response via the mitogen-activated protein kinase signaling pathways in a chicken macrophage cell line.
Poultry science
Hong Y,Lee J,Vu TH,Lee S,Lillehoj HS,Hong YH
AM4300 was used in Western Blotting to demonstrate the immunomodulation role of avian β-defensin 8 (AvBD8) in a chicken macrophage cell line.
Tue Sep 01 00:00:00 EDT 2020
Connexin43 Region 266-283, via Src Inhibition, Reduces Neural Progenitor Cell Proliferation Promoted by EGF and FGF-2 and Increases Astrocytic Differentiation.
International journal of molecular sciences
Talaverón R,Matarredona ER,Herrera A,Medina JM,Tabernero A
AM4300 was used in Western Blotting to show Src protein inhibition may contribute to the role of Cx43 in neural progenitor cells.
Mon Nov 23 00:00:00 EST 2020
Thrombomodulin Functional Domains Support Osteoblast Differentiation and Bone Healing in Diabetes in Mice.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research
Chen CH,Lai CH,Hong YK,Lu JM,Lin SY,Lee TC,Chang LY,Ho ML,Conway EM,Wu HL,Cheng TL
AM4300 was used in Western Blotting to show that osteoblast TM participates in bone healing, and recombinant TMD2/3 holds promise as a novel therapy for diabetic bone defect healing.
Tue Sep 01 00:00:00 EDT 2020
Autonomous activation of CaMKII exacerbates diastolic calcium leak during beta-adrenergic stimulation in cardiomyocytes of metabolic syndrome rats.
Cell calcium
Romero-García T,Landa-Galvan HV,Pavón N,Mercado-Morales M,Valdivia HH,Rueda A
AM4300 was used in Western Blotting to conclude that CaMKII autonomous activation in cardiomyocytes of MetS rats with central obesity significantly contributes to abnormal diastolic Ca2+ leak, increasing the propensity for β-adrenergic receptor-driven lethal arrhythmias.
Sun Nov 01 00:00:00 EDT 2020
Rigosertib-Activated JNK1/2 Eliminate Tumor Cells through p66Shc Activation.
Biology
Günther JK,Nikolajevic A,Ebner S,Troppmair J,Khalid S
AM4300 was used in Western Blotting to provide evidence that establishes p66Shc, an oxidoreductase, as a JNK1/2 effector downstream of Rigosertib-induced ROS production, DNA damage, and cell death.
Fri May 15 00:00:00 EDT 2020
miR-20b and miR-451a Are Involved in Gastric Carcinogenesis through the PI3K/AKT/mTOR Signaling Pathway: Data from Gastric Cancer Patients, Cell Lines and Ins-Gas Mouse Model.
International journal of molecular sciences
Streleckiene G,Inciuraite R,Juzenas S,Salteniene V,Steponaitiene R,Gyvyte U,Kiudelis G,Leja M,Ruzgys P,Satkauskas S,Kupcinskiene E,Franke S,Thon C,Link A,Kupcinskas J,Skieceviciene J
AM4300 was used in Western Blot to study the expression of miR-20b and miR-451a are deregulated in vitro and in vivo and have a tumor suppressive role in GC through regulation of the PI3K/AKT/mTOR signaling pathway.
Wed Jan 29 00:00:00 EST 2020
Alternative polyadenylation mediates genetic regulation of gene expression.
eLife
Mittleman BE,Pott S,Warland S,Zeng T,Mu Z,Kaur M,Gilad Y,Li Y
AM4300 was used in Western Blotting to demonstrate that alternative polyadenylation (APA) links genetic variation to variation in gene expression, protein expression, and disease risk, and reveals uncharted modes of genetic regulation.
Thu Jun 25 00:00:00 EDT 2020
Dissecting the transactivation domain (tAD) of the transcription factor c-Myb to assess recent models of tAD function.
FEBS open bio
Naes G,Storesund JO,Udayakumar PD,Ledsaak M,Gabrielsen OS
AM4300 was used in Western Blotting to revisit the tAD of the haematopoietic transcription factor c-Myb by performing a mutational analysis to see how these new models fit and potentially explain the tAD behaviour of this master regulator.
Sun Nov 01 00:00:00 EDT 2020
The SUMO Conjugase Ubc9 Protects Dopaminergic Cells from Cytotoxicity and Enhances the Stability of α-Synuclein in Parkinson's Disease Models.
eNeuro
Verma DK,Ghosh A,Ruggiero L,Cartier E,Janezic E,Williams D,Jung EG,Moore M,Seo JB,Kim YH
AM4300 was used in Western Blotting to support that SUMOylation can be a regulatory target to protect dopaminergic neurons from oxidative stress and protein aggregation, with the implication that high levels of SUMOylation in dopaminergic neurons can prevent the pathologic progression of Parkinson's disease (PD).
Fri Jun 18 00:00:00 EDT 2021
Selective hyperactivation of JNK2 in an animal model of temporal lobe epilepsy.
IBRO reports
Parikh AN,Concepcion FA,Khan MN,Boehm RD,Poolos OC,Dhami A,Poolos NP
AM4300 was used in Western Blotting to investigate the timeline of JNK activation during epileptogenesis, and to identify the JNK isoform(s) that undergo hyperactivation in the chronic epilepsy stage.
Mon Jun 01 00:00:00 EDT 2020
Sex-specific features of spine densities in the hippocampus.
Scientific reports
Brandt N,Löffler T,Fester L,Rune GM
AM4300 was used in Western Blotting to indicate that hippocampal neurons are differentiated in a sex-dependent manner, this differentiation being likely to develop during the perinatal period.
Thu Jul 09 00:00:00 EDT 2020
Nuclear Import of the HIV-1 Core Precedes Reverse Transcription and Uncoating.
Cell reports
Selyutina A,Persaud M,Lee K,KewalRamani V,Diaz-Griffero F
AM4300 was used in Western Blotting to explore the importance of the importance of the nuclear capsid for HIV-1 infection.
Tue Sep 29 00:00:00 EDT 2020
Paired Box 9 (PAX9), the RNA polymerase II transcription factor, regulates human ribosome biogenesis and craniofacial development.
PLoS genetics
Farley-Barnes KI,Deniz E,Overton MM,Khokha MK,Baserga SJ
AM4300 was used in Western Blotting to reveal another connection between craniofacial development and making ribosomes through the protein Paired Box 9 (PAX9).
Sat Aug 01 00:00:00 EDT 2020
Metformin and Everolimus: A Promising Combination for Neuroendocrine Tumors Treatment.
Cancers
Vitali E,Boemi I,Tarantola G,Piccini S,Zerbi A,Veronesi G,Baldelli R,Mazziotti G,Smiroldo V,Lavezzi E,Spada A,Mantovani G,Lania AG
AM4300 was used in Western Blotting to investigate the effects of the metformin and everolimus combination on NET cell proliferation, apoptosis, colony formation, cell viability, NET spheroids growth and the involvement of the Akt and mTOR pathways, and also developed everolimus-resistant NET cells to further study this combination.
Sun Aug 02 00:00:00 EDT 2020
Phenotypic Screen with TSC-Deficient Neurons Reveals Heat-Shock Machinery as a Druggable Pathway for mTORC1 and Reduced Cilia.
Cell reports
Di Nardo A,Lenoël I,Winden KD,Rühmkorf A,Modi ME,Barrett L,Ercan-Herbst E,Venugopal P,Behne R,Lopes CAM,Kleiman RJ,Bettencourt-Dias M,Sahin M
AM4300 was used in Western Blotting to examine whether neuronal cilia are affected in TSC.
Tue Jun 23 00:00:00 EDT 2020
Piperlongumine Acts as an Immunosuppressant by Exerting Prooxidative Effects in Human T Cells Resulting in Diminished TH17 but Enhanced Treg Differentiation.
Frontiers in immunology
Liang J,Ziegler JD,Jahraus B,Orlik C,Blatnik R,Blank N,Niesler B,Wabnitz G,Ruppert T,Hübner K,Balta E,Samstag Y
AM4300 was used in Western Blot to investigate the effects of piperlongumine on the survival and function of primary human peripheral blood T cells.
Thu Apr 01 00:00:00 EDT 2021
Metabolic syndrome diminishes insulin-induced Akt activation and causes a redistribution of Akt-interacting proteins in cardiomyocytes.
PloS one
Landa-Galvan HV,Rios-Castro E,Romero-Garcia T,Rueda A,Olivares-Reyes JA
AM4300 was used in Western Blotting to report in cardiomyocytes of MetS rats, diminished insulin-induced glucose uptake and Akt activation, evaluated by its impaired mobilization towards the plasma membrane and phosphorylation, and reflected in a re-distribution of its interacting proteins, assessed by label-free mass spectrometry.
Tue Apr 14 00:00:00 EDT 2020
Exercise rejuvenates quiescent skeletal muscle stem cells in old mice through restoration of Cyclin D1.
Nature metabolism
Brett JO,Arjona M,Ikeda M,Quarta M,de Morrée A,Egner IM,Perandini LA,Ishak HD,Goshayeshi A,Benjamin DI,Both P,Rodríguez-Mateo C,Betley MJ,Wyss-Coray T,Rando TA
AM4300 was used in Western Blotting to investigate the effect of exercise on aged stem cell function in the context of tissue regeneration in old mice.
Wed Apr 01 00:00:00 EDT 2020
A cytoskeleton regulator AVIL drives tumorigenesis in glioblastoma.
Nature communications
Xie Z,Janczyk PŁ,Zhang Y,Liu A,Shi X,Singh S,Facemire L,Kubow K,Li Z,Jia Y,Schafer D,Mandell JW,Abounader R,Li H
AM4300 was used in Western Blotting to reveal a connection between cytoskeleton and FOXM1, and establish a signalling axis of AVIL/FOXM1/LIN28B/let-7.
Fri Jul 10 00:00:00 EDT 2020
Insights into herpesvirus assembly from the structure of the pUL7:pUL51 complex.
eLife
Butt BG,Owen DJ,Jeffries CM,Ivanova L,Hill CH,Houghton JW,Ahmed MF,Antrobus R,Svergun DI,Welch JJ,Crump CM,Graham SC
AM4300 was used in Western Blotting to provide a structural framework for understanding the role of the conserved pUL7:pUL51 complex in herpesvirus assembly.
Mon May 11 00:00:00 EDT 2020
Sex differences in hypothalamic-pituitary-adrenal axis regulation after chronic unpredictable stress.
Brain and behavior
Palumbo MC,Dominguez S,Dong H
AM4300 was used in Western Blotting to investigate the behavioral and biochemical responses of the HPA axis in C57BL/6 mice.
Wed Apr 01 00:00:00 EDT 2020
Systematic Identification of Regulators of Oxidative Stress Reveals Non-canonical Roles for Peroxisomal Import and the Pentose Phosphate Pathway.
Cell reports
Dubreuil MM,Morgens DW,Okumoto K,Honsho M,Contrepois K,Lee-McMullen B,Traber GM,Sood RS,Dixon SJ,Snyder MP,Fujiki Y,Bassik MC
AM4300 was used in Western Blotting to provide insights into the roles of peroxisomal matrix import and the pentose phosphate pathway in redox biology.
Tue Feb 04 00:00:00 EST 2020
Mitochondrial Dysfunction Is an Early Consequence of Partial or Complete Dystrophin Loss in mdx Mice.
Frontiers in physiology
Moore TM,Lin AJ,Strumwasser AR,Cory K,Whitney K,Ho T,Ho T,Lee JL,Rucker DH,Nguyen CQ,Yackly A,Mahata SK,Wanagat J,Stiles L,Turcotte LP,Crosbie RH,Zhou Z
AM4300 was used in Western Blotting to indicate a mitochondrial and metabolic phenotype in both male and female mdx mice prior to the onset of muscle fiber abnormalities, potentially suggesting an early mitochondrial role in the etiology of this disease.
Wed May 03 00:00:00 EDT 2023
TRPV4 disrupts mitochondrial transport and causes axonal degeneration via a CaMKII-dependent elevation of intracellular Ca2.
Nature communications
Woolums BM,McCray BA,Sung H,Tabuchi M,Sullivan JM,Ruppell KT,Yang Y,Mamah C,Aisenberg WH,Saavedra-Rivera PC,Larin BS,Lau AR,Robinson DN,Xiang Y,Wu MN,Sumner CJ,Lloyd TE
AM4300 was used in Western Blotting to highlight an integral role for CaMKII in neuronal TRPV4-associated Ca2+ responses, the importance of tightly regulated Ca2+ dynamics for mitochondrial axonal transport, and the therapeutic promise of TRPV4 antagonists for patients with TRPV4-related neurodegenerative diseases.
Fri May 29 00:00:00 EDT 2020
An ACE2 Microbody Containing a Single Immunoglobulin Fc Domain Is a Potent Inhibitor of SARS-CoV-2.
Cell reports
Tada T,Fan C,Chen JS,Kaur R,Stapleford KA,Gristick H,Dcosta BM,Wilen CB,Nimigean CM,Landau NR
AM4300 was used in Western Blot to report an improved soluble angiotensin-converting enzyme 2 (ACE2), termed a "microbody," in which the ACE2 ectodomain is fused to Fc domain 3 of the immunoglobulin (Ig) heavy chain.
Tue Dec 22 00:00:00 EST 2020
The Minor Matrix Protein VP24 from Ebola Virus Lacks Direct Lipid-Binding Properties.
Viruses
Su Y,Stahelin RV
AM4300 was used in Western Blotting to demonstrate that the presence of the EBOV major matrix protein VP40 did not promote VP24 membrane association in vitro or in cells.
Sat Aug 08 00:00:00 EDT 2020
Cyclophilin A Prevents HIV-1 Restriction in Lymphocytes by Blocking Human TRIM5α Binding to the Viral Core.
Cell reports
Selyutina A,Persaud M,Simons LM,Bulnes-Ramos A,Buffone C,Martinez-Lopez A,Scoca V,Di Nunzio F,Hiatt J,Marson A,Krogan NJ,Hultquist JF,Diaz-Griffero F
AM4300 was used in Western Blotting to show that inhibition of HIV-1 infection caused by disrupting CypA-capsid interactions is dependent on human tripartite motif 5α (TRIM5αhu), showing that TRIM5αhu restricts HIV-1 in CD4+ T cells.
Tue Mar 17 00:00:00 EDT 2020
Low Dose of β-Carotene Regulates Inflammation, Reduces Caspase Signaling, and Correlates with Autophagy Activation in Cardiomyoblast Cell Lines.
Medical science monitor basic research
Lesmana R,Felia Yusuf I,Goenawan H,Achadiyani A,Khairani AF,Nur Fatimah S,Supratman U
AM4300 was used in Western Blotting to show that specific low-dose BC is effective and might improve cell viability by stimulating autophagy, inhibiting proinflammatory factors, and suppressing apoptosis.
Mon Dec 28 00:00:00 EST 2020
Temporal Proteomic Analysis of Herpes Simplex Virus 1 Infection Reveals Cell-Surface Remodeling via pUL56-Mediated GOPC Degradation.
Cell reports
Soh TK,Davies CTR,Muenzner J,Hunter LM,Barrow HG,Connor V,Bouton CR,Smith C,Emmott E,Antrobus R,Graham SC,Weekes MP,Crump CM
AM4300 was used in Western Blot to provide significant resources for future investigation of HSV-host interactions and highlights an efficient mechanism whereby a single virus protein targets a cellular trafficking factor to modify the surface of infected cells.
Tue Oct 06 00:00:00 EDT 2020
Rab27a Contributes to the Processing of Inflammatory Pain in Mice.
Cells
Gross T,Wack G,Syhr KMJ,Tolmachova T,Seabra MC,Geisslinger G,Niederberger E,Schmidtko A,Kallenborn-Gerhardt W
AM4300 was used in Western Blotting to uncover a previously unrecognized function of Rab27a in the processing of persistent inflammatory pain in mice.
Thu Jun 18 00:00:00 EDT 2020
The absence of (TCAGGG)n repeats in some telomeres, combined with variable responses to NR2F2 depletion, suggest that this nuclear receptor plays an indirect role in the alternative lengthening of telomeres.
Scientific reports
Alhendi ASN,Royle NJ
AM4300 was used in Western Blotting to suggest that NR2F2 does not play a direct role in ALT and we speculate about an alternative role for this NR in a DNA damage response at telomeres.
Thu Nov 26 00:00:00 EST 2020
A substrate-trapping strategy to find E3 ubiquitin ligase substrates identifies Parkin and TRIM28 targets.
Communications biology
Watanabe M,Saeki Y,Takahashi H,Ohtake F,Yoshida Y,Kasuga Y,Kondo T,Yaguchi H,Suzuki M,Ishida H,Tanaka K,Hatakeyama S
AM4300 was used in Western Blotting to develop an E3 ligase substrate-trapping technique that can identify substrates of E3 ligases.
Tue Oct 20 00:00:00 EDT 2020
Systemic Delivery of AAV-Fdxr Mitigates the Phenotypes of Mitochondrial Disorders in Fdxr Mutant Mice.
Molecular therapy. Methods & clinical development
Yang L,Slone J,Zou W,Queme LF,Jankowski MP,Yin F,Huang T
AM4300 was used in Western Blotting to provides substantial support for the translation of AAV-based Fdxr gene therapy into clinical applications.
Fri Sep 11 00:00:00 EDT 2020
Light-regulated allosteric switch enables temporal and subcellular control of enzyme activity.
eLife
Shaaya M,Fauser J,Zhurikhina A,Conage-Pough JE,Huyot V,Brennan M,Flower CT,Matsche J,Khan S,Natarajan V,Rehman J,Kota P,White FM,Tsygankov D,Karginov AV
AM4300 was used in Western Blot to demonstrate efficient regulation of the kinase and identify temporally distinct signaling responses ranging from seconds to minutes.
Wed Sep 23 00:00:00 EDT 2020
Gut butyrate-producing organisms correlate to Placenta Specific 8 protein: Importance to colorectal cancer progression.
Journal of advanced research
Huang CC,Shen MH,Chen SK,Yang SH,Liu CY,Guo JW,Chang KW,Huang CJ
AM4300 was used in Western Blotting to clarify the roles of PLAC8 in colorectal cancer tumorigenesis, invasion, and migration.
Sun Mar 01 00:00:00 EST 2020
AlCl3 exposure regulates neuronal development by modulating DNA modification.
World journal of stem cells
Cheng XJ,Guan FL,Li Q,Dai G,Li HF,Li XK
AM4300 was used in Western Blotting to study the effects of aluminum on epigenetic modification in neural stem cells and neurons.
Thu Nov 26 00:00:00 EST 2020
The Role of miR-375-3p and miR-200b-3p in Gastrointestinal Stromal Tumors.
International journal of molecular sciences
Gyvyte U,Lukosevicius R,Inciuraite R,Streleckiene G,Gudoityte G,Bekampyte J,Valentini S,Salteniene V,Ruzgys P,Satkauskas S,Zviniene K,Kupcinskas J,Skieceviciene J
AM4300 was used in Western Blotting to investigate the role of two miRNAs strongly downregulated in GIST-miR-375-3p and miR-200b-3p-in the pathogenesis of GIST.
Tue Jul 21 00:00:00 EDT 2020
A tripartite cooperative mechanism confers resistance of the protein kinase A catalytic subunit to dephosphorylation.
The Journal of biological chemistry
Chan TO,Armen RS,Yadav S,Shah S,Zhang J,Tiegs BC,Keny N,Blumhof B,Deshpande DA,Rodeck U,Penn RB
AM4300 was used in Western Blotting to establish that three allosteric sites located at the PKAc N and C termini coordinately regulate the phosphatase sensitivity of this enzyme.
Fri Mar 06 00:00:00 EST 2020
Caveolin 1 is required for axonal outgrowth of motor neurons and affects Xenopus neuromuscular development.
Scientific reports
Breuer M,Berger H,Borchers A
AM4300 was used in Western Blotting to find that loss-of-function of Xenopus Cav1 results in a striking swimming defect characterized by paralysis of the morphants.
Mon Oct 05 00:00:00 EDT 2020
Long non-coding RNA DBH-AS1 promotes cancer progression in diffuse large B-cell lymphoma by targeting FN1 via RNA-binding protein BUD13.
Cell biology international
Song Y,Gao F,Peng Y,Yang X
AM4300 was used in Western Blotting to find that long non-coding RNA dopamine β hydroxylase antisense RNA 1 is upregulated in DLBC tissues and cells, and the DBH-AS1/BUD13/FN1 axis regulates disease progression.
Mon Jun 01 00:00:00 EDT 2020
The β-catenin/YAP signaling axis is a key regulator of melanoma-associated fibroblasts.
Signal transduction and targeted therapy
Liu T,Zhou L,Yang K,Iwasawa K,Kadekaro AL,Takebe T,Andl T,Zhang Y
AM4300 was used in Western Blotting to report that β-catenin is indispensable for the activation of primary human stromal fibroblasts and the mediation of fibroblast-melanoma cell interactions.
Fri Jan 29 00:00:00 EST 2021
Systematic Identification of Host Cell Regulators of Legionella pneumophila Pathogenesis Using a Genome-wide CRISPR Screen.
Cell host & microbe
Jeng EE,Bhadkamkar V,Ibe NU,Gause H,Jiang L,Chan J,Jian R,Jimenez-Morales D,Stevenson E,Krogan NJ,Swaney DL,Snyder MP,Mukherjee S,Bassik MC
AM4300 was used in Western Blotting to characterize C1orf43 and KIAA1109 as regulators of phagocytosis and show that RAB10 and its chaperone RABIF are required for optimal L. pneumophila replication and ER recruitment to the LCV.
Wed Oct 09 00:00:00 EDT 2019
Myc and Dnmt1 impede the pluripotent to totipotent state transition in embryonic stem cells.
Nature cell biology
Fu X,Wu X,Djekidel MN,Zhang Y
AM4300 was used in Western Blotting to show the totipotent state transition in mouse embryonic stem cells is impeded by Myc-mediated downregulation of pluripotent genes and Dnmt-1-mediated prevention of 2-cell-embryo-specific gene activation.
Mon Jul 01 00:00:00 EDT 2019
Cholesterol Metabolism Is a Druggable Axis that Independently Regulates Tau and Amyloid-β in iPSC-Derived Alzheimer's Disease Neurons.
Cell stem cell
van der Kant R,Langness VF,Herrera CM,Williams DA,Fong LK,Leestemaker Y,Steenvoorden E,Rynearson KD,Brouwers JF,Helms JB,Ovaa H,Giera M,Wagner SL,Bang AG,Goldstein LSB
AM4300 was used in Western Blotting to perform a phenotypic screen for pTau accumulation in Alzheimer's disease-patient iPSC-derived neurons and identify cholesteryl esters as upstream regulators of Tau early during AD development.
Thu Mar 07 00:00:00 EST 2019
Cleavage factor 25 deregulation contributes to pulmonary fibrosis through alternative polyadenylation.
The Journal of clinical investigation
Weng T,Ko J,Masamha CP,Xia Z,Xiang Y,Chen NY,Molina JG,Collum S,Mertens TC,Luo F,Philip K,Davies J,Huang J,Wilson C,Thandavarayan RA,Bruckner BA,Jyothula SS,Volcik KA,Li L,Han L,Li W,Assassi S,Karmouty-Quintana H,Wagner EJ,Blackburn MR
AM4300 was used in Western Blotting to study the downregulation of CFIm25 in the lungs of patients with idiopathic pulmonary fibrosis and mice with pulmonary fibrosis.
Wed May 01 00:00:00 EDT 2019
Analysis of the Human Kinome and Phosphatome by Mass Cytometry Reveals Overexpression-Induced Effects on Cancer-Related Signaling.
Molecular cell
Lun XK,Szklarczyk D,Gábor A,Dobberstein N,Zanotelli VRT,Saez-Rodriguez J,von Mering C,Bodenmiller B
AM4300 was used in Mass cytometry to reveal a drug-resistant mechanism through which overexpression of tyrosine kinases, including SRC, FES, YES1, and BLK, induced MEK-independent ERK activation in melanoma A375 cells.
Thu Jun 06 00:00:00 EDT 2019
Developmental Xist induction is mediated by enhanced splicing.
Nucleic acids research
Stork C,Li Z,Lin L,Zheng S
AM4300 was used in Western Blotting to study the role of enhanced splicing in regulating developmental Xist induction.
Wed Feb 20 00:00:00 EST 2019
Acute depletion of CTCF directly affects MYC regulation through loss of enhancer-promoter looping.
Nucleic acids research
Hyle J,Zhang Y,Wright S,Xu B,Shao Y,Easton J,Tian L,Feng R,Xu P,Li C
AM4300 was used in Western Blotting to integrate the miniAID-mClover3 cassette to the endogenous CTCF locus in a human pediatric B-ALL cell line, SEM, and an immortal erythroid precursor cell line, HUDEP-2, to allow for acute depletion of CTCF protein by the auxin-inducible degron system.
Fri Jul 26 00:00:00 EDT 2019
A protein quality control pathway regulated by linear ubiquitination.
The EMBO journal
van Well EM,Bader V,Patra M,Sánchez-Vicente A,Meschede J,Furthmann N,Schnack C,Blusch A,Longworth J,Petrasch-Parwez E,Mori K,Arzberger T,Trümbach D,Angersbach L,Showkat C,Sehr DA,Berlemann LA,Goldmann P,Clement AM,Behl C,Woerner AC,Saft C,Wurst W,Haass C,Ellrichmann G,Gold R,Dittmar G,Hipp MS,Hartl FU,Tatzelt J,Winklhofer KF
AM4300 was used in Western Blotting to show the linear ubiquitin chain assembly complex modifies disease-associated protein aggregates.
Thu May 02 00:00:00 EDT 2019
ERp18 regulates activation of ATF6α during unfolded protein response.
The EMBO journal
Oka OB,van Lith M,Rudolf J,Tungkum W,Pringle MA,Bulleid NJ
AM4300 was used in Western Blotting to demonstrate that ERp18 monitors ATF6α ER quality control to ensure optimal processing following trafficking to the Golgi.
Thu Aug 01 00:00:00 EDT 2019
Autophagosome immunoisolation from GFP-LC3B mouse tissue.
Autophagy
Yao J,Qiu Y,Jia L,Zacks DN
AM4300 was used in Western Blotting to describe a protocol for rapid and efficient enrichment of autophagosomes from various tissues of the GFP-LC3 mouse.
Fri Feb 01 00:00:00 EST 2019
Cross-Talk between Receptor Tyrosine Kinases AXL and ERBB3 Regulates Invadopodia Formation in Melanoma Cells.
Cancer research
Revach OY,Sandler O,Samuels Y,Geiger B
AM4300 was used in Western Blotting to explore the role of the tyrosine kinome in the formation of invadopodia in metastatic melanoma cells.
Wed May 15 00:00:00 EDT 2019
Activation of PP2A and Inhibition of mTOR Synergistically Reduce MYC Signaling and Decrease Tumor Growth in Pancreatic Ductal Adenocarcinoma.
Cancer research
Allen-Petersen BL,Risom T,Feng Z,Wang Z,Jenny ZP,Thoma MC,Pelz KR,Morton JP,Sansom OJ,Lopez CD,Sheppard B,Christensen DJ,Ohlmeyer M,Narla G,Sears RC
AM4300 was used in Western Blotting to study whether kinase inhibition and phosphatase activation could reduce tumor growth by reducing oncogenic survival.
Tue Jan 01 00:00:00 EST 2019
Endothelial TFEB (Transcription Factor EB) Restrains IKK (IκB Kinase)-p65 Pathway to Attenuate Vascular Inflammation in Diabetic db/db Mice.
Arteriosclerosis, thrombosis, and vascular biology
Song W,Zhang CL,Gou L,He L,Gong YY,Qu D,Zhao L,Jin N,Chan TF,Wang L,Tian XY,Luo JY,Huang Y
AM4300 was used in Western Blotting to study the role of transcription factor EB in the regulation of endothelial inflammation in diabetes.
Mon Apr 01 00:00:00 EDT 2019
Recurrent somatic BRAF insertion (p.V504_R506dup): a tumor marker and a potential therapeutic target in pilocytic astrocytoma.
Oncogene
Khater F,Langlois S,Cassart P,Roy AM,Lajoie M,Healy J,Richer C,St-Onge P,Piché N,Perreault S,Cellot S,Marzouki M,Jabado N,Sinnett D
AM4300 was used in Western Blotting to emphasize the importance of BRAF alterations in PA and the need to characterize them in a given tumor as this can affect therapeutic strategies and their potential use as tumor marker in molecular diagnostics.
Mon Apr 01 00:00:00 EDT 2019
LPA Induces Keratinocyte Differentiation and Promotes Skin Barrier Function through the LPAR1/LPAR5-RHO-ROCK-SRF Axis.
The Journal of investigative dermatology
Sumitomo A,Siriwach R,Thumkeo D,Ito K,Nakagawa R,Tanaka N,Tanabe K,Watanabe A,Kishibe M,Ishida-Yamamoto A,Honda T,Kabashima K,Aoki J,Narumiya S
AM4300 was used in Western Blotting to show a previously unsuspected role for lysophosphatidic acid and its downstream signaling in the maintenance of skin homeostasis, which may serve as a novel therapeutic target for skin barrier dysfunction.
Wed May 01 00:00:00 EDT 2019
Dorsal convergence of gastrula cells requires Vangl2 and an adhesion protein-dependent change in protrusive activity.
Development (Cambridge, England)
Prince DJ,Jessen JR
AM4300 was used in Western Blotting to examine the transition to PCP-dependent mesodermal cell migration to determine the types of membrane protrusions involved and their regulation by Vangl2.
Mon Nov 25 00:00:00 EST 2019
p75 Neurotrophin Receptor Activation Regulates the Timing of the Maturation of Cortical Parvalbumin Interneuron Connectivity and Promotes Juvenile-like Plasticity in Adult Visual Cortex.
The Journal of neuroscience : the official journal of the Society for Neuroscience
Baho E,Chattopadhyaya B,Lavertu-Jolin M,Mazziotti R,Awad PN,Chehrazi P,Groleau M,Jahannault-Talignani C,Vaucher E,Ango F,Pizzorusso T,Baroncelli L,Di Cristo G
AM4300 was used in Western Blotting to reveal a new p75NTR function in the regulation of the time course of PV cell maturation and in limiting cortical plasticity.
Wed Jun 05 00:00:00 EDT 2019
Insights into lipid accumulation in skeletal muscle in dysferlin-deficient mice.
Journal of lipid research
Agarwal AK,Tunison K,Mitsche MA,McDonald JG,Garg A
AM4300 was used in Western Blotting to report a marked increase in adipocytes in the psoas and gluteus muscles but not in the soleus and tibialis anterior muscles in aged dysferlin(DYSF)-null (Bla/J)(Bla/J) mice compared with WT mice.
Sun Dec 01 00:00:00 EST 2019
LXRα limits TGFβ-dependent hepatocellular carcinoma associated fibroblast differentiation.
Oncogenesis
Morén A,Bellomo C,Tsubakihara Y,Kardassis D,Mikulits W,Heldin CH,Moustakas A
AM4300 was used in Western Blotting to determine the role of liver X receptors during myofibroblast differentiation.
Thu May 16 00:00:00 EDT 2019
m6A Regulates Neurogenesis and Neuronal Development by Modulating Histone Methyltransferase Ezh2.
Genomics, proteomics & bioinformatics
Chen J,Zhang YC,Huang C,Shen H,Sun B,Cheng X,Zhang YJ,Yang YG,Shu Q,Yang Y,Li X
AM4300 was used in Western Blotting to uncover a crosstalk between RNA and histone modifications and indicate that Mettl3-mediated m6A modification plays an important role in regulating neurogenesis and neuronal development through modulating Ezh2.
Mon Apr 01 00:00:00 EDT 2019
Aggregated and Hyperstable Damage-Associated Molecular Patterns Are Released During ER Stress to Modulate Immune Function.
Frontiers in cell and developmental biology
Andersohn A,Garcia MI,Fan Y,Thompson MC,Akimzhanov AM,Abdullahi A,Jeschke MG,Boehning D
AM4300 was used in Western Blot to show that induction of chronic ER stress leads to the release of known and novel damage-associated molecular patterns.
Mon Sep 28 00:00:00 EDT 2020
Liver-specific knockdown of long-chain acyl-CoA synthetase 4 reveals its key role in VLDL-TG metabolism and phospholipid synthesis in mice fed a high-fat diet.
American journal of physiology. Endocrinology and metabolism
Singh AB,Kan CFK,Kraemer FB,Sobel RA,Liu J
AM4300 was used in Western Blotting to investigate the acute effects of hepatic ACSL4 deficiency on lipid metabolism in adult mice fed a high-fat diet (HFD).
Wed May 01 00:00:00 EDT 2019
A role for phosphatidylcholine and phosphatidylethanolamine in hepatic insulin signaling.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
van der Veen JN,Lingrell S,McCloskey N,LeBlond ND,Galleguillos D,Zhao YY,Curtis JM,Sipione S,Fullerton MD,Vance DE,Jacobs RL
AM4300 was used in Western Blotting to show that treatments to reduce the PC:PE ratio in liver may protect against the development of hepatic insulin resistance.
Mon Apr 01 00:00:00 EDT 2019
Sensory nerves mediate spontaneous behaviors in addition to inflammation in a murine model of psoriasis.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
Kodji X,Arkless KL,Kee Z,Cleary SJ,Aubdool AA,Evans E,Caton P,Pitchford SC,Brain SD
AM4300 was used in Western Blotting to study the involvement of sensory nerve mediators with a murine model of psoriasis and associated spontaneous behaviours, indicative of cutaneous discomfort.
Fri Feb 01 00:00:00 EST 2019
Adenosine and hyaluronan promote lung fibrosis and pulmonary hypertension in combined pulmonary fibrosis and emphysema.
Disease models & mechanisms
Collum SD,Molina JG,Hanmandlu A,Bi W,Pedroza M,Mertens TCJ,Wareing N,Wei W,Wilson C,Sun W,Rajadas J,Bollyky PL,Philip KM,Ren D,Thandavarayan RA,Bruckner BA,Xia Y,Blackburn MR,Karmouty-Quintana H
AM4300 was used in Western Blotting to generate pre-clinical data demonstrating the capacity of 4MU, an FDA-approved drug, to attenuate features of CPFE in an experimental model of chronic lung injury.
Wed May 15 00:00:00 EDT 2019
A novel human Cdh1 mutation impairs anaphase promoting complex/cyclosome activity resulting in microcephaly, psychomotor retardation, and epilepsy.
Journal of neurochemistry
Rodríguez C,Sánchez-Morán I,Álvarez S,Tirado P,Fernández-Mayoralas DM,Calleja-Pérez B,Almeida Á,Fernández-Jaén A
AM4300 was used in Western Blotting to indicate that the loss of function of APC/C-Cdh1 caused by Cdh1 Asp187Gly mutation is a new cause of prenatal microcephaly, psychomotor retardation, and severe epilepsy.
Tue Oct 01 00:00:00 EDT 2019
Human antigen R mediated post-transcriptional regulation of inhibitors of apoptosis proteins in pancreatic cancer.
World journal of gastroenterology
Lukosiute-Urboniene A,Jasukaitiene A,Silkuniene G,Barauskas V,Gulbinas A,Dambrauskas Z
AM4300 was used in Western Blotting to determine the association of human antigen R and inhibitors of apoptosis proteins and prognosis in pancreatic cancer.
Mon Jan 14 00:00:00 EST 2019
Interplay between post-translational cyclooxygenase-2 modifications and the metabolic and proteomic profile in a colorectal cancer cohort.
World journal of gastroenterology
Prieto P,Jaén RI,Calle D,Gómez-Serrano M,Núñez E,Fernández-Velasco M,Martín-Sanz P,Alonso S,Vázquez J,Cerdán S,Peinado MÁ,Boscá L
AM4300 was used in Western Blotting to study the expression profile of cyclo-oxygenase 2 in the tumour tissue of colorectal cancer patients compared to the adjacent non-tumour area.
Mon Jan 28 00:00:00 EST 2019
cGMP-dependent protein kinase II determines β-catenin accumulation that is essential for uterine decidualization in mice.
American journal of physiology. Cell physiology
Zhang Y,Yan L,Liu J,Cui S,Qiu J
AM4300 was used in Western Blotting to demonstrate that estrogen and progesterone upregulate the expression of Prkg2 in uterine stromal cells depending on ER and PR; Prkg2 promotes phosphorylation of GSK-3β at serine-9 and inactivates it, leading to the accumulation of β-catenin and promoting the process of decidualization.
Sun Dec 01 00:00:00 EST 2019
Effects of Neddylation and mTOR Inhibition in Acute Myelogenous Leukemia.
Translational oncology
Guo N,Azadniv M,Coppage M,Nemer M,Mendler J,Becker M,Liesveld J
AM4300 was used in Western Blotting to evaluate the effects of pevonedistat and sapanisertib as single agents or in combination on acute myeloid leukaemia cell lines.
Mon Apr 01 00:00:00 EDT 2019
PK-M2-mediated metabolic changes in breast cancer cells induced by ionizing radiation.
Breast cancer research and treatment
Zhang L,Bailleul J,Yazal T,Dong K,Sung D,Dao A,Gosa L,Nathanson D,Bhat K,Duhachek-Muggy S,Alli C,Dratver MB,Pajonk F,Vlashi E
AM4300 was used in Western Blotting to determine the effect of ionizing radiation (IR) on M2 isoform of pyruvate kinase(PK-M2)-mediated metabolic changes in triple negative breast cancers(TNBC) cells, and their survival.
Fri Nov 01 00:00:00 EDT 2019
A time-resolved live cell imaging assay to identify small molecule inhibitors of FGF2 signaling.
FEBS letters
Ahmed M,Legrand C,Yagüe Relimpio A,Beretta CA,Muschko A,Wegehingel S,Müller HM,Sehr P,Will DW,Lewis JD,Nickel W
AM4300 was used in Western Blot to describe a novel time-resolved FGF2 signaling assay based upon live cell imaging of neuroblastoma cells.
Thu Aug 01 00:00:00 EDT 2019
Sulforaphane-induced epigenetic regulation of Nrf2 expression by DNA methyltransferase in human Caco-2 cells.
Oncology letters
Zhou JW,Wang M,Sun NX,Qing Y,Yin TF,Li C,Wu D
AM4300 was used in Western Blotting to demonstrate that sulforaphane could promote demethylation of Nrf2 promotor region, inducing chemoprevention of colon cancer.
Sun Sep 01 00:00:00 EDT 2019
Stimulated upregulation of HO-1 is associated with inadequate response of gastric and ovarian cancer cell lines to hyperthermia and cisplatin treatment.
Oncology letters
Cesna V,Sukovas A,Jasukaitiene A,Silkuniene G,Paskauskas S,Dambrauskas Z,Gulbinas A
AM4300 was used in Western Blotting to clarify the response of gastric and ovarian cancer cells to hyperthermic intraperitoneal chemotherapy, following the modulation of HO-1 expression.
Thu Aug 01 00:00:00 EDT 2019
HDAC3 and HDAC8 are required for cilia assembly and elongation.
Biology open
Park SA,Yoo H,Seol JH,Rhee K
AM4300 was used in Western Blotting to report on the role of histone deacetylases and show that they are required for the assembly and elongation of the primary cilia.
Fri Aug 09 00:00:00 EDT 2019
E-cadherin, actin, microtubules and FAK dominate different spheroid formation phases and important elements of tissue integrity.
Biology open
Smyrek I,Mathew B,Fischer SC,Lissek SM,Becker S,Stelzer EHK
AM4300 was used in Western Blotting to investigate spheroid formation dynamics in cell lines of different metastatic potential.
Thu Jan 24 00:00:00 EST 2019
Glucocorticoid-Induced Autophagy Protects Osteocytes Against Oxidative Stress Through Activation of MAPK/ERK Signaling.
JBMR plus
Kar R,Riquelme MA,Hua R,Jiang JX
AM4300 was used in Western Blotting to study the effect of glucocorticoid induced autophagy on oxidative stress in osteocytes.
Mon Apr 01 00:00:00 EDT 2019
Caveolin-1α regulates primary cilium length by controlling RhoA GTPase activity.
Scientific reports
Rangel L,Bernabé-Rubio M,Fernández-Barrera J,Casares-Arias J,Millán J,Alonso MA,Correas I
AM4300 was used in Western Blotting to study the mechanism by which caveolin-1α regulates primary cilium length.
Mon Feb 04 00:00:00 EST 2019
Modeling Glycan Processing Reveals Golgi-Enzyme Homeostasis upon Trafficking Defects and Cellular Differentiation.
Cell reports
Fisher P,Spencer H,Thomas-Oates J,Wood AJ,Ungar D
AM4300 was used in Western Blotting to develop and validate a computational model for glycan biosynthesis to probe how the biosynthetic machinery creates different glycan profiles.
Tue Apr 23 00:00:00 EDT 2019
Identification of TEX101-associated Proteins Through Proteomic Measurement of Human Spermatozoa Homozygous for the Missense Variant rs35033974.
Molecular & cellular proteomics : MCP
Schiza C,Korbakis D,Jarvi K,Diamandis EP,Drabovich AP
AM4300 was used in Western Blotting to identify TEX101-associated proteins through proteomic measurement of human spermatozoa homozygous for the missense variant rs35033974.
Fri Feb 01 00:00:00 EST 2019
BAG3 and SYNPO (synaptopodin) facilitate phospho-MAPT/Tau degradation via autophagy in neuronal processes.
Autophagy
Ji C,Tang M,Zeidler C,Höhfeld J,Johnson GV
AM4300 was used in Immunocytochemistry to demonstrate for the first time that BAG3 plays an important role in autophagic flux in the neurites of mature neurons (20-24 days in vitro [DIV]) through interaction with the post-synaptic cytoskeleton protein SYNPO (synaptopodin).
Mon Jul 01 00:00:00 EDT 2019
CIBER-CLAP (CIBERCV Cardioprotection Large Animal Platform): A multicenter preclinical network for testing reproducibility in cardiovascular interventions.
Scientific reports
Rossello X,Rodriguez-Sinovas A,Vilahur G,Crisóstomo V,Jorge I,Zaragoza C,Zamorano JL,Bermejo J,Ordoñez A,Boscá L,Vázquez J,Badimón L,Sánchez-Margallo FM,Fernández-Avilés F,Garcia-Dorado D,Ibanez B
AM4300 was used in Western Blotting to evaluate experimental reproducibility before stepping into the clinical arena.
Mon Dec 30 00:00:00 EST 2019
Enhancer of Zeste Homologue 2 Inhibition Attenuates TGF-β Dependent Hepatic Stellate Cell Activation and Liver Fibrosis.
Cellular and molecular gastroenterology and hepatology
Martin-Mateos R,De Assuncao TM,Arab JP,Jalan-Sakrikar N,Yaqoob U,Greuter T,Verma VK,Mathison AJ,Cao S,Lomberk G,Mathurin P,Urrutia R,Huebert RC,Shah VH
AM4300 was used in Western Blotting to analyse the epigenetic modulators that differentially control Transforming Growth Factor-Β and platelet-derived growth factor signalling pathways.
Mon Apr 29 00:00:00 EDT 2019
Role of Ryanodine Type 2 Receptors in Elementary Ca2+ Signaling in Arteries and Vascular Adaptive Responses.
Journal of the American Heart Association
Kaßmann M,Szijártó IA,García-Prieto CF,Fan G,Schleifenbaum J,Anistan YM,Tabeling C,Shi Y,le Noble F,Witzenrath M,Huang Y,Markó L,Nelson MT,Gollasch M
AM4300 was used in Western Blotting to determine the impact of individual ryanodine receptors isoforms on adaptive vascular responses.
Tue May 07 00:00:00 EDT 2019
GSK-3β protects fetal oocytes from premature death via modulating TAp63 expression in mice.
BMC biology
Wen J,Yan H,He M,Zhang T,Mu X,Wang H,Zhang H,Xia G,Wang C
AM4300 was used in Western Blotting to study the underlying mechanisms governing foetal oocyte attrition.
Tue Mar 12 00:00:00 EDT 2019
Metabolic Changes in Spontaneously Hypertensive Rat Hearts Precede Cardiac Dysfunction and Left Ventricular Hypertrophy.
Journal of the American Heart Association
Li J,Kemp BA,Howell NL,Massey J,Mińczuk K,Huang Q,Chordia MD,Roy RJ,Patrie JT,Davogustto GE,Kramer CM,Epstein FH,Carey RM,Taegtmeyer H,Keller SR,Kundu BK
AM4300 was used in Western Blotting to study metabolic changes in spontaneously hypertensive rats during early hypertension development, compared with control Wistar Kyoto rats.
Tue Feb 19 00:00:00 EST 2019
TYK2 promotes malignant peripheral nerve sheath tumor progression through inhibition of cell death.
Cancer medicine
Qin W,Godec A,Zhang X,Zhu C,Shao J,Tao Y,Bu X,Hirbe AC
AM4300 was used in Western Blotting to illustrate the importance of TYK2 in MPNST pathogenesis and suggest that the TYK2 pathway may be a potential therapeutic target for these deadly cancers.
Sun Sep 01 00:00:00 EDT 2019
Identification of intracellular cavin target proteins reveals cavin-PP1alpha interactions regulate apoptosis.
Nature communications
McMahon KA,Wu Y,Gambin Y,Sierecki E,Tillu VA,Hall T,Martel N,Okano S,Moradi SV,Ruelcke JE,Ferguson C,Yap AS,Alexandrov K,Hill MM,Parton RG
AM4300 was used in Western Blotting to identify a pro-apoptotic signaling pathway from surface caveolae to the nucleus.
Mon Jul 22 00:00:00 EDT 2019
Inhibition of Indoleamine 2,3 Dioxygenase Does Not Improve Cancer-Related Symptoms in a Murine Model of Human Papilloma Virus-Related Head and Neck Cancer.
International journal of tryptophan research : IJTR
Vichaya EG,Vermeer DW,Budac D,Lee A,Grossberg A,Vermeer PD,Lee JH,Dantzer R
AM4300 was used in Western Blotting to determine whether indoleamine 2,3 dioxygenase (IDO) inhibition, intended to facilitate tumor clearance in response to treatment, attenuates behavioral alterations associated with tumor growth and treatment.
Thu Oct 01 00:00:00 EDT 2020
Ifit1 regulates norovirus infection and enhances the interferon response in murine macrophage-like cells.
Wellcome open research
Mears HV,Emmott E,Chaudhry Y,Hosmillo M,Goodfellow IG,Sweeney TR
AM4300 was used in Western Blotting to study the role of Ifit1 in murine macrophage like cells.
Sun Jan 10 00:00:00 EST 2021
CRH Promotes the Neurogenic Activity of Neural Stem Cells in the Adult Hippocampus.
Cell reports
Koutmani Y,Gampierakis IA,Polissidis A,Ximerakis M,Koutsoudaki PN,Polyzos A,Agrogiannis G,Karaliota S,Thomaidou D,Rubin LL,Politis PK,Karalis KP
AM4300 was used in Western Blotting to suggest a critical role of CRH in adult neurogenesis, independently of its stress-related systemic function.
Tue Oct 22 00:00:00 EDT 2019
miR-150-504-519d inhibits the growth of human colorectal cancer cell line SW48 and downregulates c-FLIP receptor.
Journal of cellular biochemistry
Rong G,Yang X,Wu H,Wu Y
AM4300 was used in Western Blotting to demonstrate upregulation of c-FLIP in CRC, which was downregulated in SW48 cells after the transfection of miR-150-504-519d, suggesting that manipulation of miR-150-504-519d expression might be a novel approach for the treatment of colorectal cancer.
Wed May 01 00:00:00 EDT 2019
Biallelic mutation in MYH7 and MYBPC3 leads to severe cardiomyopathy with left ventricular noncompaction phenotype.
Human mutation
Kolokotronis K,Kühnisch J,Klopocki E,Dartsch J,Rost S,Huculak C,Mearini G,Störk S,Carrier L,Klaassen S,Gerull B
AM4300 was used in Western Blotting to present three cases carrying a loss-of-function (LoF) variant in a compound heterozygous state with a missense variant in either MYH7 or MYBPC3 leading to severe cardiomyopathy with left ventricular noncompaction.
Thu Aug 01 00:00:00 EDT 2019
Alteration of Autophagy Gene Expression by Different Intensity of Exercise in Gastrocnemius and Soleus Muscles of Wistar Rats.
Journal of sports science & medicine
Tarawan VM,Gunadi JW,Lesmana R,Goenawan H,Meilina DE,Sipayung JA,Wargasetia TL,Widowati W,Limyati Y,Supratman U
AM4300 was used in Western Blotting to determine if varied intensity exercises have different impacts on autophagy gene expression in soleus and gastrocnemius muscles of wistar rats.
Fri Mar 01 00:00:00 EST 2019
Shifting the balance of autophagy and proteasome activation reduces proteotoxic cell death: a novel therapeutic approach for restoring photoreceptor homeostasis.
Cell death & disease
Qiu Y,Yao J,Jia L,Thompson DA,Zacks DN
AM4300 was used in Western Blotting to support the conclusion that normalizing the A:P ratio, either by reducing the ERS-induced activation of autophagy, or by increasing proteasome activity, improves photoreceptor survival.
Thu Jul 18 00:00:00 EDT 2019
EphA4 loss improves social memory performance and alters dendritic spine morphology without changes in amyloid pathology in a mouse model of Alzheimer's disease.
Alzheimer's research & therapy
Poppe L,Rué L,Timmers M,Lenaerts A,Storm A,Callaerts-Vegh Z,Courtand G,de Boer A,Smolders S,Van Damme P,Van Den Bosch L,D'Hooge R,De Strooper B,Robberecht W,Lemmens R
AM4300 was used in Western Blotting to investigate whether EphA4 ablation might preserve synapse function and ameliorate cognitive performance in the APPPS1 transgenic mouse model of AD.
Thu Dec 12 00:00:00 EST 2019
Genomic tagging of endogenous human ESCRT-I complex preserves ESCRT-mediated membrane-remodeling functions.
The Journal of biological chemistry
Hoffman HK,Fernandez MV,Groves NS,Freed EO,van Engelenburg SB
AM4300 was used in Western Blotting to use CRISPR/Cas9-mediated knock-in to tag the critical ESCRT-I component tumor susceptibility 101 (Tsg101) with GFP at its native locus.
Fri Nov 01 00:00:00 EDT 2019
A Mechanism Linking Two Known Vulnerability Factors for Alcohol Abuse: Heightened Alcohol Stimulation and Low Striatal Dopamine D2 Receptors.
Cell reports
Bocarsly ME,da Silva E Silva D,Kolb V,Luderman KD,Shashikiran S,Rubinstein M,Sibley DR,Dobbs LK,Alvarez VA
AM4300 was used in Western Blot to demonstrate a link between two vulnerability factors for alcohol abuse and offer evidence for a mechanism in which low striatal D2Rs trigger D1R hypersensitivity, ultimately leading to compulsive-like drinking.
Tue Oct 29 00:00:00 EDT 2019
The priming factor CAPS1 regulates dense-core vesicle acidification by interacting with rabconnectin3β/WDR7 in neuroendocrine cells.
The Journal of biological chemistry
Crummy E,Mani M,Thellman JC,Martin TFJ
AM4300 was used in Western Blotting to determine how CAPS1 binds to dense-core vesicles and the role of CAPS1 in the regulation of the pH of dense-core vesicles.
Fri Jun 14 00:00:00 EDT 2019
Gigaxonin E3 ligase governs ATG16L1 turnover to control autophagosome production.
Nature communications
Scrivo A,Codogno P,Bomont P
AM4300 was used in Western Blotting to unveil the fundamental mechanisms of the control of autophagosome formation, and provide a molecular switch to fine-tune the activation of autophagy.
Fri Feb 15 00:00:00 EST 2019
GALNT3 Maintains the Epithelial State in Trophoblast Stem Cells.
Cell reports
Raghu D,Mobley RJ,Shendy NAM,Perry CH,Abell AN
AM4300 was used in Western Blotting to determine the biological relevance of GALNT3 in trophoblast stem cells.
Tue Mar 26 00:00:00 EDT 2019
Time-Variant SRC Kinase Activation Determines Endothelial Permeability Response.
Cell chemical biology
Klomp JE,Shaaya M,Matsche J,Rebiai R,Aaron JS,Collins KB,Huyot V,Gonzalez AM,Muller WA,Chew TL,Malik AB,Karginov AV
AM4300 was used in Western Blot to control tyrosine kinase SRC activation to investigate endothelial barrier enhancement via SRC required phosphorylation of VE cadherin at Y731.
Thu Aug 15 00:00:00 EDT 2019
Impaired Activity of Ryanodine Receptors Contributes to Calcium Mishandling in Cardiomyocytes of Metabolic Syndrome Rats.
Frontiers in physiology
Fernández-Miranda G,Romero-Garcia T,Barrera-Lechuga TP,Mercado-Morales M,Rueda A
AM4300 was used in Western Blotting to show the impaired in situ activity of RyR2 may also account for the poor overall cardiac outcome reported in MetS patients; hence, the SERCA pump and RyR2 are both attractive potential targets for future therapies.
Thu Oct 01 00:00:00 EDT 2020
Quantitating the epigenetic transformation contributing to cholesterol homeostasis using Gaussian process.
Nature communications
Wang C,Scott SM,Subramanian K,Loguercio S,Zhao P,Hutt DM,Farhat NY,Porter FD,Balch WE
AM4300 was used in Western Blotting to understand the impact of epigenetics on human misfolding disease.
Thu Nov 07 00:00:00 EST 2019
Basal expression of interferon regulatory factor 1 drives intrinsic hepatocyte resistance to multiple RNA viruses.
Nature microbiology
Yamane D,Feng H,Rivera-Serrano EE,Selitsky SR,Hirai-Yuki A,Das A,McKnight KL,Misumi I,Hensley L,Lovell W,González-López O,Suzuki R,Matsuda M,Nakanishi H,Ohto-Nakanishi T,Hishiki T,Wauthier E,Oikawa T,Morita K,Reid LM,Sethupathy P,Kohara M,Whitmire JK,Lemon SM
AM4300 was used in Western Blotting to map critical host pathways restricting positive-strand RNA virus replication in immortalized hepatocytes and identify a role for IRF1.
Mon Jul 01 00:00:00 EDT 2019
Musashi interaction with poly(A)-binding protein is required for activation of target mRNA translation.
The Journal of biological chemistry
Cragle CE,MacNicol MC,Byrum SD,Hardy LL,Mackintosh SG,Richardson WA,Gray NK,Childs GV,Tackett AJ,MacNicol AM
AM4300 was used in Western Blotting to investigate the molecular events that distinguish Musashi-mediated translational activation from repression.
Fri Jul 12 00:00:00 EDT 2019
Impaired Hepatic Phosphatidylcholine Synthesis Leads to Cholestasis in Mice Challenged With a High-Fat Diet.
Hepatology communications
Wan S,Kuipers F,Havinga R,Ando H,Vance DE,Jacobs RL,van der Veen JN
AM4300 was used in Western Blotting to investigate whether phosphatidylethanolamine N-methyltransferase is an important supplier of phosphatidylcholine to the canaliculus and its role in maintaining canalicular membrane integrity and bile formation following high-fat diet feeding.
Fri Feb 01 00:00:00 EST 2019
The impact of exercise on mitochondrial dynamics and the role of Drp1 in exercise performance and training adaptations in skeletal muscle.
Molecular metabolism
Moore TM,Zhou Z,Cohn W,Norheim F,Lin AJ,Kalajian N,Strumwasser AR,Cory K,Whitney K,Ho T,Ho T,Lee JL,Rucker DH,Shirihai O,van der Bliek AM,Whitelegge JP,Seldin MM,Lusis AJ,Lee S,Drevon CA,Mahata SK,Turcotte LP,Hevener AL
AM4300 was used in Western Blotting to compare the ability of Clostridioides difficile spores to germinate, produce toxin and cause tissue damage in a bioengineered 3D tissue model versus in a 2D transwell model in which human cells form a polarised monolayer.
Fri Mar 01 00:00:00 EST 2019
Mitigation of off-target toxicity in CRISPR-Cas9 screens for essential non-coding elements.
Nature communications
Tycko J,Wainberg M,Marinov GK,Ursu O,Hess GT,Ego BK,Li A,Truong A,Trevino AE,Spees K,Yao D,Kaplow IM,Greenside PG,Morgens DW,Phanstiel DH,Snyder MP,Bintu L,Greenleaf WJ,Kundaje A,Bassik MC
AM4300 was used in Western Blotting to study off targets effects of essential non-coding element of Crispr-Cas9.
Fri Sep 06 00:00:00 EDT 2019
Cytoplasmic DAXX drives SQSTM1/p62 phase condensation to activate Nrf2-mediated stress response.
Nature communications
Yang Y,Willis TL,Button RW,Strang CJ,Fu Y,Wen X,Grayson PRC,Evans T,Sipthorpe RJ,Roberts SL,Hu B,Zhang J,Lu B,Luo S
AM4300 was used in Western Blotting to suggests a mechanism of p62 phase condensation by a protein interaction, and indicates that DAXX regulates redox homoeostasis, providing a mechanistic insight into the prosurvival function of DAXX.
Wed Aug 21 00:00:00 EDT 2019
Targeting PFKFB3 alleviates cerebral ischemia-reperfusion injury in mice.
Scientific reports
Burmistrova O,Olias-Arjona A,Lapresa R,Jimenez-Blasco D,Eremeeva T,Shishov D,Romanov S,Zakurdaeva K,Almeida A,Fedichev PO,Bolaños JP
AM4300 was used in Western Blotting to show that selective inhibition of PFKFB3 activity by the small molecule AZ67 prevents the NADPH oxidation, redox stress and apoptotic cell death caused by the activation of glycolysis triggered upon excitotoxic and oxygen-glucose deprivation/reoxygenation models in mouse primary neurons.
Mon Aug 12 00:00:00 EDT 2019
Bone Morphogenetic Protein 9 Regulates Early Lymphatic-Specified Endothelial Cell Expansion during Mouse Embryonic Stem Cell Differentiation.
Stem cell reports
Subileau M,Merdzhanova G,Ciais D,Collin-Faure V,Feige JJ,Bailly S,Vittet D
AM4300 was used in Western Blotting to examine the first steps of lymphatic specification and expansion.
Tue Jan 08 00:00:00 EST 2019
Region-specific inhibition of 14-3-3 proteins induces psychomotor behaviors in mice.
NPJ schizophrenia
Graham K,Zhang J,Qiao H,Wu Y,Zhou Y
AM4300 was used in Western Blotting to study the association between the inhibition of the 14-3-3 family of proteins in specific forebrain regions with certain schizophrenia-associated endophenotypes.
Mon Jan 14 00:00:00 EST 2019
Fatty Acid Synthesis and Degradation Interplay to Regulate the Oxidative Stress in Cancer Cells.
International journal of molecular sciences
Mikalayeva V,Ceslevičienė I,Sarapinienė I,Žvikas V,Skeberdis VA,Jakštas V,Bordel S
AM4300 was used in Western Blotting to study whether fatty acid synthesis and fatty acid oxidation coexist in breast cancer cells.
Mon Mar 18 00:00:00 EDT 2019
Decreased Expression of Cilia Genes in Pancreatic Islets as a Risk Factor for Type 2 Diabetes in Mice and Humans.
Cell reports
Kluth O,Stadion M,Gottmann P,Aga H,Jähnert M,Scherneck S,Vogel H,Krus U,Seelig A,Ling C,Gerdes J,Schürmann A
AM4300 was used in Western Blot to determine the role of primary cilia on compensatory beta cell replication.
Tue Mar 12 00:00:00 EDT 2019
Clinical resistance to crenolanib in acute myeloid leukemia due to diverse molecular mechanisms.
Nature communications
Zhang H,Savage S,Schultz AR,Bottomly D,White L,Segerdell E,Wilmot B,McWeeney SK,Eide CA,Nechiporuk T,Carlos A,Henson R,Lin C,Searles R,Ho H,Lam YL,Sweat R,Follit C,Jain V,Lind E,Borthakur G,Garcia-Manero G,Ravandi F,Kantarjian HM,Cortes J,Collins R,Buelow DR,Baker SD,Druker BJ,Tyner JW
AM4300 was used in Western Blotting to investigate the mechanisms of crenolanib resistance in acute myeloid leukaemia.
Wed Jan 16 00:00:00 EST 2019
Reducing EphA4 before disease onset does not affect survival in a mouse model of Amyotrophic Lateral Sclerosis.
Scientific reports
Rué L,Timmers M,Lenaerts A,Smolders S,Poppe L,de Boer A,Van Den Bosch L,Van Damme P,Robberecht W,Lemmens R
AM4300 was used in Western Blotting to gain more insights in the cell-specific role of decreasing EphA4 expression in addition to timing and amount of EphA4 reduction.
Tue Oct 01 00:00:00 EDT 2019
Mitochondrial DNA Stress Signalling Protects the Nuclear Genome.
Nature metabolism
Wu Z,Oeck S,West AP,Mangalhara KC,Sainz AG,Newman LE,Zhang XO,Wu L,Yan Q,Bosenberg M,Liu Y,Sulkowski PL,Tripple V,Kaech SM,Glazer PM,Shadel GS
AM4300 was used in Western Blotting to better understand the nature and downstream consequences of innate immune signalling due to endogenous mtDNA stress.
Sun Dec 01 00:00:00 EST 2019
Lemon Peel Polyphenol Extract Reduces Interleukin-6-Induced Cell Migration, Invasiveness, and Matrix Metalloproteinase-9/2 Expression in Human Gastric Adenocarcinoma MKN-28 and AGS Cell Lines.
Biomolecules
Pagliara V,Nasso R,Di Donato P,Finore I,Poli A,Masullo M,Arcone R
AM4300 was used in Western Blotting to analyze the anti-cancer properties of LPE on migration and invasiveness in MKN-28 and AGS human gastric cancer cell lines either in the absence or presence of the pro-inflammatory cytokine IL-6.
Thu Dec 05 00:00:00 EST 2019
Obesity Drives STAT-1-Dependent NASH and STAT-3-Dependent HCC.
Cell
Grohmann M,Wiede F,Dodd GT,Gurzov EN,Ooi GJ,Butt T,Rasmiena AA,Kaur S,Gulati T,Goh PK,Treloar AE,Archer S,Brown WA,Muller M,Watt MJ,Ohara O,McLean CA,Tiganis T
AM4300 was used in Immunohistochemistry to reveal how obesity-associated hepatic oxidative stress can independently contribute to the pathogenesis of non-alcoholic steatohepatitis, fibrosis, and hepatocellular carcinoma.
Thu Nov 15 00:00:00 EST 2018
P300 Acetyltransferase Mediates Stiffness-Induced Activation of Hepatic Stellate Cells Into Tumor-Promoting Myofibroblasts.
Gastroenterology
Dou C,Liu Z,Tu K,Zhang H,Chen C,Yaqoob U,Wang Y,Wen J,van Deursen J,Sicard D,Tschumperlin D,Zou H,Huang WC,Urrutia R,Shah VH,Kang N
AM4300 was used in Western Blotting to investigate whether stiffness due to the presence of tumours increases activation of hepatic stellate cells into myofibroblasts and their tumour-promoting effects.
Fri Jun 01 00:00:00 EDT 2018
Small Intestine Microbiota Regulate Host Digestive and Absorptive Adaptive Responses to Dietary Lipids.
Cell host & microbe
Martinez-Guryn K,Hubert N,Frazier K,Urlass S,Musch MW,Ojeda P,Pierre JF,Miyoshi J,Sontag TJ,Cham CM,Reardon CA,Leone V,Chang EB
AM4300 was used in Western Blotting to study the contribution of the small bowel microbiota of mammals to diet-microbe interactions, including lipid metabolism and absorption.
Wed Apr 11 00:00:00 EDT 2018
Partial loss of psychiatric risk gene Mir137 in mice causes repetitive behavior and impairs sociability and learning via increased Pde10a.
Nature neuroscience
Cheng Y,Wang ZM,Tan W,Wang X,Li Y,Bai B,Li Y,Zhang SF,Yan HL,Chen ZL,Liu CM,Mi TW,Xia S,Zhou Z,Liu A,Tang GB,Liu C,Dai ZJ,Wang YY,Wang H,Wang X,Kang Y,Lin L,Chen Z,Xie N,Sun Q,Xie W,Peng J,Chen D,Teng ZQ,Jin P
AM4300 was used in Western Blotting to examine the impact of miR-137 loss-of-function in vivo.
Sat Dec 01 00:00:00 EST 2018
The Transcription Factor ThPOK Orchestrates Stochastic Interchromosomal Interactions Required for IFNB1 Virus-Inducible Gene Expression.
Molecular cell
Nikopoulou C,Panagopoulos G,Sianidis G,Psarra E,Ford E,Thanos D
AM4300 was used in Western Blotting to show that the transcription factor ThPOK binds cooperatively with NF-κB to NRCs and mediates their physical proximity with the IFNB1 gene via its ability to oligomerize when bound to DNA.
Thu Jul 19 00:00:00 EDT 2018
The structure of human Nocturnin reveals a conserved ribonuclease domain that represses target transcript translation and abundance in cells.
Nucleic acids research
Abshire ET,Chasseur J,Bohn JA,Del Rizzo PA,Freddolino PL,Goldstrohm AC,Trievel RC
AM4300 was used in Western Blotting to study the mechanisms by which Nocturnin regulates high-fat diet induced weight gain, and bone formation.
Fri Jul 06 00:00:00 EDT 2018
SAMMSON fosters cancer cell fitness by concertedly enhancing mitochondrial and cytosolic translation.
Nature structural & molecular biology
Vendramin R,Verheyden Y,Ishikawa H,Goedert L,Nicolas E,Saraf K,Armaos A,Delli Ponti R,Izumikawa K,Mestdagh P,Lafontaine DLJ,Tartaglia GG,Takahashi N,Marine JC,Leucci E
AM4300 was used in Western Blotting to highlight how a single oncogenic lncRNA can simultaneously modulate RNA-protein complex formation in two distinct cellular compartments to promote cell growth.
Thu Nov 01 00:00:00 EDT 2018
HDAC6 is a therapeutic target in mutant GARS-induced Charcot-Marie-Tooth disease.
Brain : a journal of neurology
Benoy V,Van Helleputte L,Prior R,d'Ydewalle C,Haeck W,Geens N,Scheveneels W,Schevenels B,Cader MZ,Talbot K,Kozikowski AP,Vanden Berghe P,Van Damme P,Robberecht W,Van Den Bosch L
AM4300 was used in Western Blotting to demonstrate that α-tubulin deacetylation and disrupted axonal transport may represent a common pathogenic mechanism underlying Charcot-Marie-Tooth disease.
Thu Mar 01 00:00:00 EST 2018
p110α of PI3K is necessary and sufficient for quiescence exit in adult muscle satellite cells.
The EMBO journal
Wang G,Zhu H,Situ C,Han L,Yu Y,Cheung TH,Liu K,Wu Z
AM4300 was used in Western Blotting to investigate the extrinsic cues and intrinsic signalling pathways that regulate quiescence exit during adult mouse muscle satellite cell activation.
Fri Apr 13 00:00:00 EDT 2018
Continuous signaling of CD79b and CD19 is required for the fitness of Burkitt lymphoma B cells.
The EMBO journal
He X,Kläsener K,Iype JM,Becker M,Maity PC,Cavallari M,Nielsen PJ,Yang J,Reth M
AM4300 was used in Western Blotting to show that Igβ and CD19 are part of an alternative B-cell signalling module that use continuous ITAM/PI3K signalling to promote the survival of B lymphoma and normal B cells.
Fri Jun 01 00:00:00 EDT 2018
RAPGEF5 Regulates Nuclear Translocation of β-Catenin.
Developmental cell
Griffin JN,Del Viso F,Duncan AR,Robson A,Hwang W,Kulkarni S,Liu KJ,Khokha MK
AM4300 was used in Western Blotting to study the mechanisms regulating Β-catenin nuclear transport.
Mon Jan 22 00:00:00 EST 2018
FOXO are required for intervertebral disk homeostasis during aging and their deficiency promotes disk degeneration.
Aging cell
Alvarez-Garcia O,Matsuzaki T,Olmer M,Miyata K,Mokuda S,Sakai D,Masuda K,Asahara H,Lotz MK
AM4300 was used in Western Blotting to investigate the function of FOXO in intervertebral disk ageing and age-related degeneration.
Mon Oct 01 00:00:00 EDT 2018
Menin regulates the serine biosynthetic pathway in Ewing sarcoma.
The Journal of pathology
Svoboda LK,Teh SSK,Sud S,Kerk S,Zebolsky A,Treichel S,Thomas D,Halbrook CJ,Lee HJ,Kremer D,Zhang L,Klossowski S,Bankhead AR,Magnuson B,Ljungman M,Cierpicki T,Grembecka J,Lyssiotis CA,Lawlor ER
AM4300 was used in Western Blotting to study the mechanistic basis of menin-MLL-mediated oncogenic activity in Ewing sarcoma.
Sun Jul 01 00:00:00 EDT 2018
Mitochondrial redox sensing by the kinase ATM maintains cellular antioxidant capacity.
Science signaling
Zhang Y,Lee JH,Paull TT,Gehrke S,D'Alessandro A,Dou Q,Gladyshev VN,Schroeder EA,Steyl SK,Christian BE,Shadel GS
AM4300 was used in Western Blotting to propose that loss of the mitochondrial ROS-sensing function of ATM may cause cellular ROS accumulation and oxidative stress in A-T.
Tue Jul 10 00:00:00 EDT 2018
Synthetic lethality of TNK2 inhibition in PTPN11-mutant leukemia.
Science signaling
Jenkins C,Luty SB,Maxson JE,Eide CA,Abel ML,Togiai C,Nemecek ER,Bottomly D,McWeeney SK,Wilmot B,Loriaux M,Chang BH,Tyner JW
AM4300 was used in Western Blotting to suggest that TNK2 is a promising therapeutic target for PTPN11-mutant leukemias.
Tue Jul 17 00:00:00 EDT 2018
Oxidative stress and mitochondrial dynamics malfunction are linked in Pelizaeus-Merzbacher disease.
Brain pathology (Zurich, Switzerland)
Ruiz M,Bégou M,Launay N,Ranea-Robles P,Bianchi P,López-Erauskin J,Morató L,Guilera C,Petit B,Vaurs-Barriere C,Guéret-Gonthier C,Bonnet-Dupeyron MN,Fourcade S,Auwerx J,Boespflug-Tanguy O,Pujol A
AM4300 was used in Western Blotting to examine the link between redox and metabolic homeostasis in myelin diseases.
Sat Sep 01 00:00:00 EDT 2018
The oxidoreductase p66Shc acts as tumor suppressor in BRAFV600E-transformed cells.
Molecular oncology
Furlan T,Khalid S,Nguyen AV,Günther J,Troppmair J
AM4300 was used in Western Blotting to suggest that phosphorylation-activated p66Shc functions as a tumor suppressor in melanoma cells.
Fri Jun 01 00:00:00 EDT 2018
CLDN18.1 attenuates malignancy and related signaling pathways of lung adenocarcinoma in vivo and in vitro.
International journal of cancer
Luo J,Chimge NO,Zhou B,Flodby P,Castaldi A,Firth AL,Liu Y,Wang H,Yang C,Marconett CN,Crandall ED,Offringa IA,Frenkel B,Borok Z
AM4300 was used in Western Blot to indicate a tumor suppressor role for CLDN18.1 in LuAd mediated by a regulatory network that encompasses YAP/TAZ, IGF-1R and AKT signaling.
Sat Dec 15 00:00:00 EST 2018
Transcriptional response to Wnt activation regulates the regenerative capacity of the mammalian cochlea.
Development (Cambridge, England)
Samarajeewa A,Lenz DR,Xie L,Chiang H,Kirchner R,Mulvaney JF,Edge ASB,Dabdoub A
AM4300 was used in Western Blotting to demonstrate that Wnt activation promotes supporting cell proliferation following birth, but prior to postnatal day 5.
Tue Nov 27 00:00:00 EST 2018
Notch1 is asymmetrically distributed from the beginning of embryogenesis and controls the ventral center.
Development (Cambridge, England)
Castro Colabianchi AM,Revinski DR,Encinas PI,Baez MV,Monti RJ,Rodríguez Abinal M,Kodjabachian L,Franchini LF,López SL
AM4300 was used in Western Blotting to suggest that ventral enrichment of Notch1 underlies the process by which Notch1 participates in restricting nuclear accumulation of Β-catenin to the dorsal side.
Tue Jul 17 00:00:00 EDT 2018
Vangl2-dependent regulation of membrane protrusions and directed migration requires a fibronectin extracellular matrix.
Development (Cambridge, England)
Love AM,Prince DJ,Jessen JR
AM4300 was used in Western Blotting to study the role of Vang-like 2 on the regulation of membrane-protusive activities in migrating gastrula cells.
Thu Nov 15 00:00:00 EST 2018
Human Adenovirus Infection Causes Cellular E3 Ubiquitin Ligase MKRN1 Degradation Involving the Viral Core Protein pVII.
Journal of virology
Inturi R,Mun K,Singethan K,Schreiner S,Punga T
AM4300 was used in Western Blotting to identify several cellular proteins interacting with the human Adenovirus precursor pVII protein.
Thu Feb 01 00:00:00 EST 2018
β2-Adrenergic receptor modulates mitochondrial metabolism and disease progression in recurrent/metastatic HPV(+) HNSCC.
Oncogenesis
Lucido CT,Callejas-Valera JL,Colbert PL,Vermeer DW,Miskimins WK,Spanos WC,Vermeer PD
AM4300 was used in Western Blotting to demonstrate that an aggressive murine model of recurrent/metastatic human papillomavirus-associated head and neck squamous cell carcinoma upregulates Β2-adrenergic receptor expression, concordant with significantly heightened mitochondrial metabolism.
Mon Oct 08 00:00:00 EDT 2018
Proteomic and Biochemical Comparison of the Cellular Interaction Partners of Human VPS33A and VPS33B.
Journal of molecular biology
Hunter MR,Hesketh GG,Benedyk TH,Gingras AC,Graham SC
AM4300 was used in Western Blotting to identify proteins associating with both VPS33A and VPS33B, suggesting that these proteins have distinct sub-cellular localisations.
Fri Jul 06 00:00:00 EDT 2018
Selection and Characterization of Rupintrivir-Resistant Norwalk Virus Replicon Cells In Vitro.
Antimicrobial agents and chemotherapy
Kitano M,Hosmillo M,Emmott E,Lu J,Goodfellow I
AM4300 was used in Western Blotting to demonstrate that mutations in the norovirus protease that lead to the generation of resistant mutants can rapidly occur.
Tue May 01 00:00:00 EDT 2018
Modulation of glutathione promotes apoptosis in triple-negative breast cancer cells.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
Miran T,Vogg ATJ,Drude N,Mottaghy FM,Morgenroth A
AM4300 was used in Western Blotting to evaluate the potential of pharmacological GSH depletion to sensitize CSCs to ionizing radiotherapy with an I-125-labeled nucleoside analog, 5-iodo-4'-thio-2'-deoxyuridine.
Tue May 01 00:00:00 EDT 2018
Excess LINC complexes impair brain morphogenesis in a mouse model of recessive TOR1A disease.
Human molecular genetics
Dominguez Gonzalez B,Billion K,Rous S,Pavie B,Lange C,Goodchild R
AM4300 was used in Immunohistochemistry to predict human TOR1A disease will include incompletely penetrant defects in embryonic brain morphogenesis in cases where mutations ablate TOR1A function.
Fri Jun 15 00:00:00 EDT 2018
Elevated dual specificity protein phosphatase 4 in cardiomyopathy caused by lamin A/C gene mutation is primarily ERK1/2-dependent and its depletion improves cardiac function and survival.
Human molecular genetics
Choi JC,Wu W,Phillips E,Plevin R,Sera F,Homma S,Worman HJ
AM4300 was used in Western Blotting to show that female LmnaH222P/H222P mice have increased Dusp4 expression and hyperactivation of extracellular signal-regulated kinase 1/2 with delayed kinetics relative to male mice, consistent with the sex-dependent delay in the onset and progression of disease.
Sun Jul 01 00:00:00 EDT 2018
Elevated HuR in Pancreas Promotes a Pancreatitis-Like Inflammatory Microenvironment That Facilitates Tumor Development.
Molecular and cellular biology
Peng W,Furuuchi N,Aslanukova L,Huang YH,Brown SZ,Jiang W,Addya S,Vishwakarma V,Peters E,Brody JR,Dixon DA,Sawicki JA
AM4300 was used in Western Blot to explore the consequences of human antigen R (HuR) overexpression using a novel transgenic mouse model that has a >2-fold elevation of pancreatic HuR expression.
Thu Feb 01 00:00:00 EST 2018
The Balance of PI3K and ERK Signaling Is Dysregulated in Prolactinoma and Modulated by Dopamine.
Endocrinology
Roof AK,Jirawatnotai S,Trudeau T,Kuzyk C,Wierman ME,Kiyokawa H,Gutierrez-Hartmann A
AM4300 was used in Western Blotting to study the mechanism by which dopamine antagonists inhibit lactotrope proliferation.
Fri Jun 01 00:00:00 EDT 2018
KCC2 Regulates Dendritic Spine Formation in a Brain-Region Specific and BDNF Dependent Manner.
Cerebral cortex (New York, N.Y. : 1991)
Awad PN,Amegandjin CA,Szczurkowska J,Carriço JN,Fernandes do Nascimento AS,Baho E,Chattopadhyaya B,Cancedda L,Carmant L,Di Cristo G
AM4300 was used in Western Blotting to investigate whether KCC2 regulation of dendritic spine development, and its underlying mechanisms, are brain-region specific.
Thu Nov 01 00:00:00 EDT 2018
A homozygous variant disrupting the PIGH start-codon is associated with developmental delay, epilepsy, and microcephaly.
Human mutation
Pagnamenta AT,Murakami Y,Anzilotti C,Titheradge H,Oates AJ,Morton J,Kinoshita T,Kini U,Taylor JC
AM4300 was used in Western Blotting to characterise a homozygous c.1A > T variant resulting in defective glycosylphosphatidylinositol-anchor biogenesis and highlight the importance of exploring low-coverage variants within autozygous regions.
Fri Jun 01 00:00:00 EDT 2018
HIF1 mediates a switch in pyruvate kinase isoforms after myocardial infarction.
Physiological genomics
Williams AL,Khadka V,Tang M,Avelar A,Schunke KJ,Menor M,Shohet RV
AM4300 was used in Western Blotting to investigate the transcriptional response after myocardial infarction and to identify novel, hypoxia-driven changes, including alternative splicing.
Sun Jul 01 00:00:00 EDT 2018
The AKAP Cypher/Zasp contributes to β-adrenergic/PKA stimulation of cardiac CaV1.2 calcium channels.
The Journal of general physiology
Yu H,Yuan C,Westenbroek RE,Catterall WA
AM4300 was used in Western Blotting to examine the role of the A-kinase anchoring protein Cypher/Zasp in Β-adrenergic regulation of CaV1.2 channels.
Mon Jun 04 00:00:00 EDT 2018
Localization to detergent-resistant membranes and HIV-1 core entry inhibition correlate with HIV-1 restriction by SERINC5.
Virology
Schulte B,Selyutina A,Opp S,Herschhorn A,Sodroski JG,Pizzato M,Diaz-Griffero F
AM4300 was used in Western Blotting to determine the exact mechanism by which SERINC5 restricts HIV-1.
Thu Feb 01 00:00:00 EST 2018
Disruption of GPR35 Exacerbates Dextran Sulfate Sodium-Induced Colitis in Mice.
Digestive diseases and sciences
Farooq SM,Hou Y,Li H,O'Meara M,Wang Y,Li C,Wang JM
AM4300 was used in Western Blotting to suggest that GPR35 could play a crucial role in protecting from colonic inflammation and serve as a therapeutic target.
Thu Nov 01 00:00:00 EDT 2018
Low temperature decreases bone mass in mice: Implications for humans.
American journal of physical anthropology
Robbins A,Tom CATMB,Cosman MN,Moursi C,Shipp L,Spencer TM,Brash T,Devlin MJ
AM4300 was used in Western Blotting to show that chronic exposure to low temperature impairs bone architecture, suggesting climate may contribute to phenotypic variation in humans and other hominins.
Thu Nov 01 00:00:00 EDT 2018
Nutmeg Extract Increases Skeletal Muscle Mass in Aging Rats Partly via IGF1-AKT-mTOR Pathway and Inhibition of Autophagy.
Evidence-based complementary and alternative medicine : eCAM
Pratiwi YS,Lesmana R,Goenawan H,Sylviana N,Setiawan I,Tarawan VM,Lestari K,Abdulah R,Dwipa L,Purba A,Supratman U
AM4300 was used in Western Blotting to study the physiological health benefit of nutmeg extract during ageing, especially in the myogenesis process in skeletal muscle.
Wed Sep 30 00:00:00 EDT 2020
Murine macrophage TLR2-FcγR synergy via FcγR licensing of IL-6 cytokine mRNA ribosome binding and translation.
PloS one
Hunt D,Drake LA,Drake JR
AM4300 was used in Western Blotting to study FcγR regulation of TLR2-driven production of IL-6.
Tue Jan 22 00:00:00 EST 2019
CRB3 and the FERM protein EPB41L4B regulate proliferation of mammary epithelial cells through the release of amphiregulin.
PloS one
Walker SJ,Selfors LM,Margolis BL,Brugge JS
AM4300 was used in Western Blotting to study the effects of ectopic expression of CRB3 in mammary epithelial cells.
Tue Apr 16 00:00:00 EDT 2019
Neuronal adaptor FE65 stimulates Rac1-mediated neurite outgrowth by recruiting and activating ELMO1.
The Journal of biological chemistry
Li W,Tam KMV,Chan WWR,Koon AC,Ngo JCK,Chan HYE,Lau KF
AM4300 was used in Western Blotting to identify a novel mechanism by which FE65 stimulates Rac1-mediated neurite outgrowth through recruiting and activating of ELMO1.
Fri May 18 00:00:00 EDT 2018
TDP43 nuclear export and neurodegeneration in models of amyotrophic lateral sclerosis and frontotemporal dementia.
Scientific reports
Archbold HC,Jackson KL,Arora A,Weskamp K,Tank EM,Li X,Miguez R,Dayton RD,Tamir S,Klein RL,Barmada SJ
AM4300 was used in Western Blotting to indicate that redundant pathways regulate TDP43 nuclear export, and prevention of cytoplasmic TDP43 accumulation may be enhanced by targeting overlapping mechanisms.
Thu Mar 15 00:00:00 EDT 2018
Anti-obesity activity of OBEX is regulated by activation of thermogenesis and decreasing adiposity gain.
Scientific reports
Carreira MC,Andrade S,Gonzalez-Izquierdo A,Amil M,Folgueira C,Monteiro MP,Sanz E,Crujeiras AB,Casanueva FF
AM4300 was used in Western Blotting to study the anti-obesity effects of OBEX, and the mechanisms governing these effects.
Wed Nov 21 00:00:00 EST 2018
Mouse Homolog of the Human TP53 R337H Mutation Reveals Its Role in Tumorigenesis.
Cancer research
Park JH,Li J,Starost MF,Liu C,Zhuang J,Chen J,Achatz MI,Kang JG,Wang PY,Savage SA,Hwang PM
AM4300 was used in Western Blot to show that the human R337H mutation can compromise p53 activity and promote tumorigenesis.
Sat Sep 15 00:00:00 EDT 2018
Adiponectin release and insulin receptor targeting share trans-Golgi-dependent endosomal trafficking routes.
Molecular metabolism
Rödiger M,Werno MW,Wilhelmi I,Baumeier C,Hesse D,Wettschureck N,Offermanns S,Song K,Krauß M,Schürmann A
AM4300 was used in Western Blotting to demonstrate the role of the trans-Golgi localised monomeric GTPase ARFRP1 in endosomal-mediated vesicle trafficking of mature adipocytes.
Thu Feb 01 00:00:00 EST 2018
Canagliflozin inhibits interleukin-1β-stimulated cytokine and chemokine secretion in vascular endothelial cells by AMP-activated protein kinase-dependent and -independent mechanisms.
Scientific reports
Mancini SJ,Boyd D,Katwan OJ,Strembitska A,Almabrouk TA,Kennedy S,Palmer TM,Salt IP
AM4300 was used in Western Blotting to examine whether sodium-glucose co-transporter-2 inhibitors attenuate inflammatory signalling in cultured human endothelial cells.
Tue Mar 27 00:00:00 EDT 2018
Rapamycin and fasting sustain autophagy response activated by ischemia/reperfusion injury and promote retinal ganglion cell survival.
Cell death & disease
Russo R,Varano GP,Adornetto A,Nazio F,Tettamanti G,Girardello R,Cianfanelli V,Cavaliere F,Morrone LA,Corasaniti MT,Cecconi F,Bagetta G,Nucci C
AM4300 was used in Western Blotting to analyse the autophagic response in the retinas of mice subjected to ischemia induced by transient elevation of intraocular pressure, reporting a biphasic and reperfusion time-dependent modulation of the process.
Mon Sep 24 00:00:00 EDT 2018
Protein phosphatase 2A stimulates activation of TFEB and TFE3 transcription factors in response to oxidative stress.
The Journal of biological chemistry
Martina JA,Puertollano R
AM4300 was used in Western Blotting to show oxidative stress induces protein phosphatase 2A-mediated activation of transcription factor EB and IGHM enhancer 3 which regulate stress pathways.
Fri Aug 10 00:00:00 EDT 2018
Nuclear transglutaminase 2 directly regulates expression of cathepsin S in rat cortical neurons.
The European journal of neuroscience
Ji C,Tang M,Harrison J,Paciorkowski A,Johnson GVW
AM4300 was used in Western Blotting to illustrate that TG2 plays a role in promoting neuron health in part by repressing the expression of cathepsin S.
Thu Nov 01 00:00:00 EDT 2018
A novel probe to assess cytosolic entry of exogenous proteins.
Nature communications
Lu Q,Grotzke JE,Cresswell P
AM4300 was used in Western Blotting to demonstrate that antigen dislocation into the cytosol is the rate limiting step in cross-presentation.
Mon Aug 06 00:00:00 EDT 2018
Sulforaphane Inhibits Inflammatory Responses of Primary Human T-Cells by Increasing ROS and Depleting Glutathione.
Frontiers in immunology
Liang J,Jahraus B,Balta E,Ziegler JD,Hübner K,Blank N,Niesler B,Wabnitz GH,Samstag Y
AM4300 was used in Western Blotting to demonstrate that sulphoraphane induces a pro-oxidative state in untransformed human T-cells of healthy donors or rheumatoid arthritis patients.
Tue Oct 08 00:00:00 EDT 2019
The α2δ-like Protein Cachd1 Increases N-type Calcium Currents and Cell Surface Expression and Competes with α2δ-1.
Cell reports
Dahimene S,Page KM,Kadurin I,Ferron L,Ho DY,Powell GT,Pratt WS,Wilson SW,Dolphin AC
AM4300 was used in Western Blotting to demonstrate that Cachd1 influences both CaV2.2 trafficking and function and can inhibit responses to α2δ-1.
Tue Nov 06 00:00:00 EST 2018
The SUMO protease SENP1 and the chromatin remodeler CHD3 interact and jointly affect chromatin accessibility and gene expression.
The Journal of biological chemistry
Rodríguez-Castañeda F,Lemma RB,Cuervo I,Bengtsen M,Moen LM,Ledsaak M,Eskeland R,Gabrielsen OS
AM4300 was used in Western Blotting to study the role of sentrin-specific protease 1 in activating gene expression.
Fri Oct 05 00:00:00 EDT 2018
The SUMO protease SENP1 and the chromatin remodeler CHD3 interact and jointly affect chromatin accessibility and gene expression.
The Journal of biological chemistry
Rodríguez-Castañeda F,Lemma RB,Cuervo I,Bengtsen M,Moen LM,Ledsaak M,Eskeland R,Gabrielsen OS
AM4300 was used in Western Blotting to study the role of sentrin-specific protease 1 in activating gene expression.
Fri Oct 05 00:00:00 EDT 2018
High Fat Diet Attenuates the Anticontractile Activity of Aortic PVAT via a Mechanism Involving AMPK and Reduced Adiponectin Secretion.
Frontiers in physiology
Almabrouk TAM,White AD,Ugusman AB,Skiba DS,Katwan OJ,Alganga H,Guzik TJ,Touyz RM,Salt IP,Kennedy S
AM4300 was used in Western Blotting to investigate the mechanisms by which a high fat diet reduces the anticontractile effect of aortic perivascular adipose tissue in mice.
Sat Jan 29 00:00:00 EST 2022
Cancer exosomes induce tumor innervation.
Nature communications
Madeo M,Colbert PL,Vermeer DW,Lucido CT,Cain JT,Vichaya EG,Grossberg AJ,Muirhead D,Rickel AP,Hong Z,Zhao J,Weimer JM,Spanos WC,Lee JH,Dantzer R,Vermeer PD
AM4300 was used in Western Blotting to study whether, in some tumours, nerves are acquired by a tumour-induced process, called axonogenesis.
Tue Oct 16 00:00:00 EDT 2018
Mouse medulloblastoma driven by CRISPR activation of cellular Myc.
Scientific reports
Vo BT,Kwon JA,Li C,Finkelstein D,Xu B,Orr BA,Sherr CJ,Roussel MF
AM4300 was used in Western Blotting to develop a mouse model of MYC-driven Group 3 medulloblastoma.
Thu Jun 07 00:00:00 EDT 2018
Caspase-mediated cleavage of the centrosomal proteins during apoptosis.
Cell death & disease
Seo MY,Rhee K
AM4300 was used in Western Blotting to demonstrate that caspase-specific cleavage of SAS-6 and pericentrin may be linked to the functional disruption of the centrosome during apoptosis.
Tue May 01 00:00:00 EDT 2018
Galectin-3 sensitized melanoma cell lines to vemurafenib (PLX4032) induced cell death through prevention of autophagy.
Oncotarget
Bustos SO,da Silva Pereira GJ,de Freitas Saito R,Gil CD,Zanatta DB,Smaili SS,Chammas R
AM4300 was used in Western Blotting to show that the autophagic flux is dependent on Gal-3 levels, which attenuate the prosurvival role of autophagy.
Tue Mar 06 00:00:00 EST 2018
Combined inhibition of PI3K and Src kinases demonstrates synergistic therapeutic efficacy in clear-cell renal carcinoma.
Oncotarget
Roelants C,Giacosa S,Pillet C,Bussat R,Champelovier P,Bastien O,Guyon L,Arnoux V,Cochet C,Filhol O
AM4300 was used in Western Blotting to demonstrate that combined inhibition of PI3K and Src impedes cell growth and invasion, and induces cell death of renal carcinoma cells.
Tue Jul 10 00:00:00 EDT 2018
Dimerization of the cellular prion protein inhibits propagation of scrapie prions.
The Journal of biological chemistry
Engelke AD,Gonsberg A,Thapa S,Jung S,Ulbrich S,Seidel R,Basu S,Multhaup G,Baier M,Engelhard M,Schätzl HM,Winklhofer KF,Tatzelt J
AM4300 was used in Western Blotting to study the effect of pathogenic mutations on dimerisation in the hydrophobic domain of the cellular prion protein.
Fri May 25 00:00:00 EDT 2018
Decreased Hippocampal Neuroplasticity and Behavioral Impairment in an Animal Model of Inhalant Abuse.
Frontiers in neuroscience
Malloul H,Bennis M,Bonzano S,Gambarotta G,Perroteau I,De Marchis S,Ba-M'hamed S
AM4300 was used in Western Blotting to demonstrate significant structural and molecular changes in the adult mice hippocampus after prolonged paint thinner inhalation.
Wed Sep 30 00:00:00 EDT 2020
Host DNA released by NETosis promotes rhinovirus-induced type-2 allergic asthma exacerbation.
Nature medicine
Toussaint M,Jackson DJ,Swieboda D,Guedán A,Tsourouktsoglou TD,Ching YM,Radermecker C,Makrinioti H,Aniscenko J,Bartlett NW,Edwards MR,Solari R,Farnir F,Papayannopoulos V,Bureau F,Marichal T,Johnston SL
AM4300 was used in Western Blotting to study how respiratory viral infections boost the type-2 immune response strongly implicated in asthma exacerbation.
Thu Jun 01 00:00:00 EDT 2017
A Novel α-Calcitonin Gene-Related Peptide Analogue Protects Against End-Organ Damage in Experimental Hypertension, Cardiac Hypertrophy, and Heart Failure.
Circulation
Aubdool AA,Thakore P,Argunhan F,Smillie SJ,Schnelle M,Srivastava S,Alawi KM,Wilde E,Mitchell J,Farrell-Dillon K,Richards DA,Maltese G,Siow RC,Nandi M,Clark JE,Shah AM,Sams A,Brain SD
AM4300 was used in Western Blotting to evaluate whether a novel acylated α-calcitonin gene-related peptide analogue can alleviate and reverse cardiovascular disease in 2 distinct murine models of hypertension and heart failure in vivo.
Tue Jul 25 00:00:00 EDT 2017
Integrated Molecular Characterization of Uterine Carcinosarcoma.
Cancer cell
Cherniack AD,Shen H,Walter V,Stewart C,Murray BA,Bowlby R,Hu X,Ling S,Soslow RA,Broaddus RR,Zuna RE,Robertson G,Laird PW,Kucherlapati R,Mills GB,Weinstein JN,Zhang J,Akbani R,Levine DA
AM4300 was used in reverse phase protein array to characterize the molecular identity of uterine carcinosarcomas.
Mon Mar 13 00:00:00 EDT 2017
Lack of Glycogenin Causes Glycogen Accumulation and Muscle Function Impairment.
Cell metabolism
Testoni G,Duran J,García-Rocha M,Vilaplana F,Serrano AL,Sebastián D,López-Soldado I,Sullivan MA,Slebe F,Vilaseca M,Muñoz-Cánoves P,Guinovart JJ
AM4300 was used in Western Blotting to study the effects of glycogenin deficiency on glycogen accumulation and muscle function in mice.
Wed Jul 05 00:00:00 EDT 2017
The Retromer Supports AMPA Receptor Trafficking During LTP.
Neuron
Temkin P,Morishita W,Goswami D,Arendt K,Chen L,Malenka R
AM4300 was used in Western Blotting to demonstrate a role for the endosomal sorting complex in the trafficking of AMPA receptors during NMDA-receptor-dependent LTP at mature hippocampal synapses.
Wed Apr 05 00:00:00 EDT 2017
TDP-43 Depletion in Microglia Promotes Amyloid Clearance but Also Induces Synapse Loss.
Neuron
Paolicelli RC,Jawaid A,Henstridge CM,Valeri A,Merlini M,Robinson JL,Lee EB,Rose J,Appel S,Lee VM,Trojanowski JQ,Spires-Jones T,Schulz PE,Rajendran L
AM4300 was used in Western Blot to investigate whether increased microglial phagocytic activity that clears amyloid can also cause pathological synapse loss.
Wed Jul 19 00:00:00 EDT 2017
Mechanism and regulation of the Lys6-selective deubiquitinase USP30.
Nature structural & molecular biology
Gersch M,Gladkova C,Schubert AF,Michel MA,Maslen S,Komander D
AM4300 was used in Western Blotting to study the mechanism and regulation of the Lys6-selective deubiquitinase USP30.
Wed Nov 01 00:00:00 EDT 2017
Role of Fibulin 3 in Aging-Related Joint Changes and Osteoarthritis Pathogenesis in Human and Mouse Knee Cartilage.
Arthritis & rheumatology (Hoboken, N.J.)
Hasegawa A,Yonezawa T,Taniguchi N,Otabe K,Akasaki Y,Matsukawa T,Saito M,Neo M,Marmorstein LY,Lotz MK
AM4300 was used in Western Blotting to examine the expression patterns of fibulin 3 in ageing-related joint changes, and determine its role in the pathogenesis of osteoarthritis.
Wed Mar 01 00:00:00 EST 2017
Role of Fibulin 3 in Aging-Related Joint Changes and Osteoarthritis Pathogenesis in Human and Mouse Knee Cartilage.
Arthritis & rheumatology (Hoboken, N.J.)
Hasegawa A,Yonezawa T,Taniguchi N,Otabe K,Akasaki Y,Matsukawa T,Saito M,Neo M,Marmorstein LY,Lotz MK
AM4300 was used in Western Blotting to examine the expression patterns of fibulin 3 in ageing-related joint changes, and determine its role in the pathogenesis of osteoarthritis.
Wed Mar 01 00:00:00 EST 2017
Glycation potentiates α-synuclein-associated neurodegeneration in synucleinopathies.
Brain : a journal of neurology
Vicente Miranda H,Szego ÉM,Oliveira LMA,Breda C,Darendelioglu E,de Oliveira RM,Ferreira DG,Gomes MA,Rott R,Oliveira M,Munari F,Enguita FJ,Simões T,Rodrigues EF,Heinrich M,Martins IC,Zamolo I,Riess O,Cordeiro C,Ponces-Freire A,Lashuel HA,Santos NC,Lopes LV,Xiang W,Jovin TM,Penque D,Engelender S,Zweckstetter M,Klucken J,Giorgini F,Quintas A,Outeiro TF
AM4300 was used in Western Blotting to demonstrate glycation constitutes a novel drug target that can be explored in synucleinopathies as well as in other neurodegenerative conditions.
Mon May 01 00:00:00 EDT 2017
Tenascin-C Is a Major Component of the Fibrogenic Niche in Kidney Fibrosis.
Journal of the American Society of Nephrology : JASN
Fu H,Tian Y,Zhou L,Zhou D,Tan RJ,Stolz DB,Liu Y
AM4300 was used in Western Blotting to determine whether tenascin-C is a component of the fibrogenic niche in kidney fibrosis.
Wed Mar 01 00:00:00 EST 2017
Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor Sequestration in Triple-Negative Breast Cancer.
Developmental cell
Bajikar SS,Wang CC,Borten MA,Pereira EJ,Atkins KA,Janes KA
AM4300 was used in Western Blotting to study the tumour-suppressive mode of action of growth-differentiation factor 11, and an unusual mechanism of its inactivation in triple negative breast cancer.
Mon Nov 20 00:00:00 EST 2017
A Molecular Switch Regulating Cell Fate Choice between Muscle Progenitor Cells and Brown Adipocytes.
Developmental cell
An Y,Wang G,Diao Y,Long Y,Fu X,Weng M,Zhou L,Sun K,Cheung TH,Ip NY,Sun H,Wang H,Wu Z
AM4300 was used in Western Blotting to examine the regulation of cell fate between muscle progenitor cells to brown adipocytes.
Mon May 22 00:00:00 EDT 2017
FGF21 improves glucose homeostasis in an obese diabetes-prone mouse model independent of body fat changes.
Diabetologia
Laeger T,Baumeier C,Wilhelmi I,Würfel J,Kamitz A,Schürmann A
AM4300 was used in Western Blotting to investigate whether exogenous fibroblast growth factor 21 is sufficient to prevent diabetes and beta cell loss in New Zealand obese mice.
Wed Nov 01 00:00:00 EDT 2017
The mechanism of sirtuin 2-mediated exacerbation of alpha-synuclein toxicity in models of Parkinson disease.
PLoS biology
de Oliveira RM,Vicente Miranda H,Francelle L,Pinho R,Szegö ÉM,Martinho R,Munari F,Lázaro DF,Moniot S,Guerreiro P,Fonseca-Ornelas L,Marijanovic Z,Antas P,Gerhardt E,Enguita FJ,Fauvet B,Penque D,Pais TF,Tong Q,Becker S,Kügler S,Lashuel HA,Steegborn C,Zweckstetter M,Outeiro TF
AM4300 was used in western blot to analyze the mechanistic relationship between sirtuin 2 and alpha-synuclein in Parkinson's disease
Wed Mar 01 00:00:00 EST 2017
Microphthalmia-associated transcription factor suppresses invasion by reducing intracellular GTP pools.
Oncogene
Bianchi-Smiraglia A,Bagati A,Fink EE,Moparthy S,Wawrzyniak JA,Marvin EK,Battaglia S,Jowdy P,Kolesnikova M,Foley CE,Berman AE,Kozlova NI,Lipchick BC,Paul-Rosner LM,Bshara W,Ackroyd JJ,Shewach DS,Nikiforov MA
AM4300 was used in western blot to study suppression invasion by reduction of intracellular GTP pools via a microphthalmia-associated transcription factor
Thu Jan 05 00:00:00 EST 2017
BAIAP3, a C2 domain-containing Munc13 protein, controls the fate of dense-core vesicles in neuroendocrine cells.
The Journal of cell biology
Zhang X,Jiang S,Mitok KA,Li L,Attie AD,Martin TFJ
AM4300 was used in Western Blotting to identify C2 domain-containing proteins involved in the regulation of Ca(2+)-dependent dense-core vesicle exocytosis in neuroendocrine cells.
Mon Jul 03 00:00:00 EDT 2017
Dysregulated circadian rhythm pathway in human osteoarthritis: NR1D1 and BMAL1 suppression alters TGF-β signaling in chondrocytes.
Osteoarthritis and cartilage
Akagi R,Akatsu Y,Fisch KM,Alvarez-Garcia O,Teramura T,Muramatsu Y,Saito M,Sasho T,Su AI,Lotz MK
AM4300 was used in western blot to study circadian rhythmicity in cultured chondrocytes and determine the role of NR1D1 and BMAL1 in regulating chondrocyte functions
Thu Jun 01 00:00:00 EDT 2017
Sex-Dependent Regulation of Aromatase-Mediated Synaptic Plasticity in the Basolateral Amygdala.
The Journal of neuroscience : the official journal of the Society for Neuroscience
Bender RA,Zhou L,Vierk R,Brandt N,Keller A,Gee CE,Schäfer MK,Rune GM
AM4300 was used in Western Blotting to indicate that neuron-derived E2 modulates synaptic plasticity in rodent basolateral amygdala (BLA) sex-dependently.
Wed Feb 08 00:00:00 EST 2017
The C Terminus of the Herpes Simplex Virus UL25 Protein Is Required for Release of Viral Genomes from Capsids Bound to Nuclear Pores.
Journal of virology
Huffman JB,Daniel GR,Falck-Pedersen E,Huet A,Smith GA,Conway JF,Homa FL
AM4300 was used in Western Blotting to identify pUL25 as a key viral factor for genome uncoating.
Tue Aug 01 00:00:00 EDT 2017
Roles of the PDZ-binding motif of HPV 16 E6 protein in oncogenic transformation of human cervical keratinocytes.
Cancer science
Yoshimatsu Y,Nakahara T,Tanaka K,Inagawa Y,Narisawa-Saito M,Yugawa T,Ohno SI,Fujita M,Nakagama H,Kiyono T
AM4300 was used in Western Blotting to understand the role of the PDZ-binding motif of HPV16 E6 protein in the oncogenic transformation of human cervical keratinocytes.
Sat Jul 01 00:00:00 EDT 2017
Hepatic Stellate Cell Selective Disruption of Dynamin-2 GTPase Increases Murine Fibrogenesis through Up-Regulation of Sphingosine-1 Phosphate-Induced Cell Migration.
The American journal of pathology
Wang R,Ding Q,De Assuncao TM,Mounajjed T,Maiers JL,Dou C,Cao S,Yaqoob U,Huebert RC,Shah VH
AM4300 was used in Western Blotting to determine whether Dynamin-2 with lysine44 converted to alanine, expression in hepatic stellate cells (HSC) would decrease HSC activation and fibrogenesis in vivo by disrupting receptor tyrosine kinase endocytosis and signalling.
Sun Jan 01 00:00:00 EST 2017
Class I HDACs control a JIP1-dependent pathway for kinesin-microtubule binding in cardiomyocytes.
Journal of molecular and cellular cardiology
Blakeslee WW,Lin YH,Stratton MS,Tatman PD,Hu T,Ferguson BS,McKinsey TA
AM4300 was used in Western Blotting to define a novel role for class I histone deacetylases in the control of the JIP1/kinesin axis in cardiomyocytes.
Wed Nov 01 00:00:00 EDT 2017
Cross-Presentation of Soluble and Cell-Associated Antigen by Murine Hepatocytes Is Enhanced by Collectrin Expression.
Journal of immunology (Baltimore, Md. : 1950)
Dolina JS,Cechova S,Rudy CK,Sung SJ,Tang WW,Lee J,Hahn YS,Le TH
AM4300 was used in Western Blotting to identify the functional role of collectrin in the liver.
Wed Mar 15 00:00:00 EDT 2017
Immunogenic Dendritic Cell Generation from Pluripotent Stem Cells by Ectopic Expression of Runx3.
Journal of immunology (Baltimore, Md. : 1950)
Takacs E,Boto P,Simo E,Csuth TI,Toth BM,Raveh-Amit H,Pap A,Kovács EG,Kobolak J,Benkö S,Dinnyes A,Szatmari I
AM4300 was used in Western Blotting to find that ex vivo-generated embryonic stem cell-derived dendritic cells have a compromised maturation ability and immunogenicity.
Sun Jan 01 00:00:00 EST 2017
RhoC regulates the actin remodeling required for phagosome formation during FcγR-mediated phagocytosis.
Journal of cell science
Egami Y,Kawai K,Araki N
AM4300 was used in Western Blotting to suggest that RhoC modulates phagosome formation be modifying actin cytoskeletal remodeling via mDia1.
Fri Dec 15 00:00:00 EST 2017
Cells lay their own tracks - optogenetic Cdc42 activation stimulates fibronectin deposition supporting directed migration.
Journal of cell science
Zimmerman SP,Asokan SB,Kuhlman B,Bear JE
AM4300 was used in Western Blotting to find that a remarkably small amount of fibronectin is necessary to support stable GTPase-driven lamellipodia formation.
Fri Sep 15 00:00:00 EDT 2017
PLK4 phosphorylation of CP110 is required for efficient centriole assembly.
Cell cycle (Georgetown, Tex.)
Lee M,Seo MY,Chang J,Hwang DS,Rhee K
AM4300 was used in Western Blotting to reveal that polo-like kinase 4 specifically phosphorylates CP110 at the S98 position.
Sun Jun 18 00:00:00 EDT 2017
Polyubiquitination of apurinic/apyrimidinic endonuclease 1 by Parkin.
Molecular carcinogenesis
Scott TL,Wicker CA,Suganya R,Dhar B,Pittman T,Horbinski C,Izumi T
AM4300 was used in western blot to study how Parkin is responsible for polyubiquitination of apurinic/apyrimidinic endonuclease 1
Wed Feb 01 00:00:00 EST 2017
Cytoplasmic poly-GA aggregates impair nuclear import of TDP-43 in C9orf72 ALS/FTLD.
Human molecular genetics
Khosravi B,Hartmann H,May S,Möhl C,Ederle H,Michaelsen M,Schludi MH,Dormann D,Edbauer D
AM4300 was used in Western Blotting to study how compact cytoplasmic poly-GA aggregates impair nuclear import of a reporter containing the TDP-43 nuclear localisation signal.
Wed Feb 15 00:00:00 EST 2017
Lamina-associated polypeptide 1 is dispensable for embryonic myogenesis but required for postnatal skeletal muscle growth.
Human molecular genetics
Shin JY,Méndez-López I,Hong M,Wang Y,Tanji K,Wu W,Shugol L,Krauss RS,Dauer WT,Worman HJ
AM4300 was used in Western Blotting to explore the potential role of lamina-associated polypeptide 1 in myogenic differentiation and development.
Sun Jan 01 00:00:00 EST 2017
Fat mass and obesity-associated (FTO) protein regulates adult neurogenesis.
Human molecular genetics
Li L,Zang L,Zhang F,Chen J,Shen H,Shu L,Liang F,Feng C,Chen D,Tao H,Xu T,Li Z,Kang Y,Wu H,Tang L,Zhang P,Jin P,Shu Q,Li X
AM4300 was used in Western Blotting to study the role of fat mass and obesity-associated gene in neurodevelopment and neurogenesis.
Sat Jul 01 00:00:00 EDT 2017
Metabolic, Reproductive, and Neurologic Abnormalities in Agpat1-Null Mice.
Endocrinology
Agarwal AK,Tunison K,Dalal JS,Nagamma SS,Hamra FK,Sankella S,Shao X,Auchus RJ,Garg A
AM4300 was used in Western Blotting to explore the physiology of 1-acylglycerol-3-phosphate O-acyltransferase in mice.
Wed Nov 01 00:00:00 EDT 2017
RIP3 antagonizes a TSC2-mediated pro-survival pathway in glioblastoma cell death.
Biochimica et biophysica acta. Molecular cell research
Fettweis G,Di Valentin E,L'homme L,Lassence C,Dequiedt F,Fillet M,Coupienne I,Piette J
AM4300 was used in Western Blotting to highlight a new role of TSC2 to protect glioblastoma against PDT-induced cell death and (ii) TSC2 and 14-3-3 as new RIP3 partners.
Sun Jan 01 00:00:00 EST 2017
MiRNA-143 mediates the proliferative signaling pathway of FSH and regulates estradiol production.
The Journal of endocrinology
Zhang L,Zhang X,Zhang X,Lu Y,Li L,Cui S
AM4300 was used in Western Blotting to indicate that miR-143 plays vital roles in FSH-induced estradiol production and granulosa cell proliferation, providing a novel mechanism that involves miRNA in regulating granulosa cell functions.
Sat Jul 01 00:00:00 EDT 2017
Sirtuin 2 Regulates Microvascular Inflammation during Sepsis.
Journal of immunology research
Buechler N,Wang X,Yoza BK,McCall CE,Vachharajani V
AM4300 was used in Western Blotting to demonstrate that SIRT2 modulates microvascular inflammation in sepsis and affects survival.
Thu Feb 08 00:00:00 EST 2018
Deletion of Spata2 by CRISPR/Cas9n causes increased inhibin alpha expression and attenuated fertility in male mice.
Biology of reproduction
Zhao J,Zhao J,Xu G,Wang Z,Gao J,Cui S,Liu J
AM4300 was used in Western Blotting to reveal the impact of SPATA2 on male fertility and suggested that SPATA2 ensures the normal secretory function of Sertoli cells.
Fri Sep 01 00:00:00 EDT 2017
Deletion of β1-integrin in collecting duct principal cells leads to tubular injury and renal medullary fibrosis.
American journal of physiology. Renal physiology
Mamuya FA,Xie D,Lei L,Huang M,Tsuji K,Capen DE,Yang B,Weissleder R,Păunescu TG,Lu HAJ
AM4300 was used in Western Blotting to study the effects of Β1-integrin deletion in collecting duct principal cells.
Sun Oct 01 00:00:00 EDT 2017
Use of a novel docosahexaenoic acid formulation vs control in a neonatal porcine model of short bowel syndrome leads to greater intestinal absorption and higher systemic levels of DHA.
Nutrition research (New York, N.Y.)
Martin CR,Stoll B,Cluette-Brown J,Akinkuotu AC,Olutoye OO,Gura KM,Singh P,Zaman MM,Perillo MC,Puder M,Freedman SD,Burrin D
AM4300 was used in Western Blotting to evaluate the systemic absorption of a novel enteral Docosahexaenoic acid formulation that forms micelles independent of bile salts.
Wed Mar 01 00:00:00 EST 2017
Rab28 is a TBC1D1/TBC1D4 substrate involved in GLUT4 trafficking.
FEBS letters
Zhou Z,Menzel F,Benninghoff T,Chadt A,Du C,Holman GD,Al-Hasani H
AM4300 was used in Western Blot to study the effect and action of Rab28 in the insulin-stimulated translocation of GLUT4.
Sun Jan 01 00:00:00 EST 2017
Phosphorylation of transcriptional regulators in the retinoblastoma protein pathway by UL97, the viral cyclin-dependent kinase encoded by human cytomegalovirus.
Virology
Iwahori S,Kalejta RF
AM4300 was used in Western Blotting to observe that UL97 phosphorylates LIN52, a component of p107- and p130-assembled transcriptionally repressive DREAM complexes that control transcription during the G0/G1 phases, and the Rb-associated E2F3 protein that activates transcription through G1 and S phases.
Fri Dec 01 00:00:00 EST 2017
Data-Modeling Identifies Conflicting Signaling Axes Governing Myoblast Proliferation and Differentiation Responses to Diverse Ligand Stimuli.
Cellular and molecular bioengineering
Loiben AM,Soueid-Baumgarten S,Kopyto RF,Bhattacharya D,Kim JC,Cosgrove BD
AM4300 was used in Western Blotting to present an integrated data-modeling approach to elucidate synergies and antagonisms among proliferation and differentiation signaling axes in mouse primary myoblasts stimulated by regeneration-associated ligands.
Sun Oct 01 00:00:00 EDT 2017
Nanoparticulate titanium dioxide-inhibited dendritic development is involved in apoptosis and autophagy of hippocampal neurons in offspring mice.
Toxicology research
Zhou Y,Hong F,Tian Y,Zhao X,Hong J,Ze Y,Wang L
AM4300 was used in Western Blotting to determine the effects of nanoparticulate titanium dioxide on the dendritic outgrowth of hippocampal neurons, and the excessive apoptosis in neurotoxicity of offspring mice.
Wed Nov 01 00:00:00 EDT 2017
Endogenous AJAP1 associates with the cytoskeleton and attenuates angiogenesis in endothelial cells.
Biology open
Hötte K,Smyrek I,Starzinski-Powitz A,Stelzer EHK
AM4300 was used in Western Blotting to suggest AJAP1 as a novel tumor suppressor and diagnostic marker.
Thu Jun 15 00:00:00 EDT 2017
Overexpression of Thioredoxin1 enhances functional recovery in a mouse model of hind limb ischemia.
The Journal of surgical research
Shaikh IA,Rishi MT,Youssef M,Selvaraju V,Thirunavukkarasu M,Ukani G,Lakshmanan R,Palesty JA,Maulik N
AM4300 was used in Western Blotting to demonstrate that Trx1 enhances blood perfusion and increases angiogenic protein expression in a rodent HLI model.
Tue Aug 01 00:00:00 EDT 2017
E-cadherin integrates mechanotransduction and EGFR signaling to control junctional tissue polarization and tight junction positioning.
Nature communications
Rübsam M,Mertz AF,Kubo A,Marg S,Jüngst C,Goranci-Buzhala G,Schauss AC,Horsley V,Dufresne ER,Moser M,Ziegler W,Amagai M,Wickström SA,Niessen CM
AM4300 was used in Western Blotting to study how E-cadherin integrates multiple mechanisms to coordinate the formation of an apical tight junction network.
Wed Nov 01 00:00:00 EDT 2017
The tumor suppressor phosphatase PP2A-B56α regulates stemness and promotes the initiation of malignancies in a novel murine model.
PloS one
Janghorban M,Langer EM,Wang X,Zachman D,Daniel CJ,Hooper J,Fleming WH,Agarwal A,Sears RC
AM4300 was used in Western Blotting to suggest that B56α is important for stem cells to maintain homeostasis and that B56α loss leads to increased oncogene activity.
Mon Jan 01 00:00:00 EST 2018
Expression of N-WASP is regulated by HiF1α through the hypoxia response element in the N-WASP promoter.
Biochemistry and biophysics reports
Salvi A,Thanabalu T
AM4300 was used in Western Blotting to demonstrate that hypoxia-induced activation of N-WASP expression is mediated by HIF1α interaction with the hypoxia response element 1(-132).
Wed Mar 01 00:00:00 EST 2017
Structural and spatial chromatin features at developmental gene loci in human pluripotent stem cells.
Nature communications
Ikeda H,Sone M,Yamanaka S,Yamamoto T
AM4300 was used in Western Blotting to indicate that higher-order chromatin regulation may be an integral part of the differentiation capacity that defines pluripotency.
Mon Nov 20 00:00:00 EST 2017
Effect of PPAR-β/δ agonist GW0742 treatment in the acute phase response and blood-brain barrier permeability following brain injury.
Translational research : the journal of laboratory and clinical medicine
Chehaibi K,le Maire L,Bradoni S,Escola JC,Blanco-Vaca F,Slimane MN
AM4300 was used in western blot to report the effects of peroxisome proliferator-activated receptor beta/delta agonist on the acute phase response after brain injury
Sat Apr 01 00:00:00 EDT 2017
A Detailed Protocol for Subcellular RNA Sequencing (subRNA-seq).
Current protocols in molecular biology
Mayer A,Churchman LS
AM4300 was used in Western Blotting to develop a step-by-step protocol for subcellular RNA-sequencing.
Mon Oct 02 00:00:00 EDT 2017
DOT1L safeguards cartilage homeostasis and protects against osteoarthritis.
Nature communications
Monteagudo S,Cornelis FMF,Aznar-Lopez C,Yibmantasiri P,Guns LA,Carmeliet P,Cailotto F,Lories RJ
AM4300 was used in Western Blotting to show that DOT1L, an enzyme involved in histone methylation, is a master protector of cartilage health.
Mon Jun 19 00:00:00 EDT 2017
Cytoplasmic cyclin D1 controls the migration and invasiveness of mantle lymphoma cells.
Scientific reports
Body S,Esteve-Arenys A,Miloudi H,Recasens-Zorzo C,Tchakarska G,Moros A,Bustany S,Vidal-Crespo A,Rodriguez V,Lavigne R,Com E,Casanova I,Mangues R,Weigert O,Sanjuan-Pla A,Menéndez P,Marcq B,Picquenot JM,Pérez-Galán P,Jardin F,Roué G,Sola B
AM4300 was used in Western Blotting to identify a role of cytoplasmic cyclin D1 in the control of mantle cell lymphoma (MCL) cell migration and invasion, and as a true operator of MCL pathogenesis.
Tue Oct 24 00:00:00 EDT 2017
A Herpesviral Immediate Early Protein Promotes Transcription Elongation of Viral Transcripts.
mBio
Fox HL,Dembowski JA,DeLuca NA
AM4300 was used in Western Blot to demonstrate the interaction of a viral protein, ICP22, with a subset of cellular proteins known to be involved in transcription elongation.
Tue Jun 13 00:00:00 EDT 2017
FLNA is implicated in pulmonary neuroendocrine tumors aggressiveness and progression.
Oncotarget
Vitali E,Boemi I,Rosso L,Cambiaghi V,Novellis P,Mantovani G,Spada A,Alloisio M,Veronesi G,Ferrero S,Lania AG
AM4300 was used in Western Blotting to demonstrate that Filamin A is implicated in pulmonary neuroendocrine tumor progression, in part through Rap1.
Fri Sep 29 00:00:00 EDT 2017
Accumulation of autophagosomes confers cytotoxicity.
The Journal of biological chemistry
Button RW,Roberts SL,Willis TL,Hanemann CO,Luo S
AM4300 was used in Immunocytochemistry to investigate the role of cytotoxicity in accumulating autophagosomes.
Fri Aug 18 00:00:00 EDT 2017
Synergic Functions of miRNAs Determine Neuronal Fate of Adult Neural Stem Cells.
Stem cell reports
Pons-Espinal M,de Luca E,Marzi MJ,Beckervordersandforth R,Armirotti A,Nicassio F,Fabel K,Kempermann G,De Pietri Tonelli D
AM4300 was used in Western Blotting to demonstrate that microRNAs are required for the generation of new neurons, but not astrocytes, in the adult murine hippocampus.
Tue Apr 11 00:00:00 EDT 2017
Sirt3 deficiency does not affect venous thrombosis or NETosis despite mild elevation of intracellular ROS in platelets and neutrophils in mice.
PloS one
Hayashi H,Cherpokova D,Martinod K,Witsch T,Wong SL,Gallant M,Cifuni SM,Guarente LP,Wagner DD
AM4300 was used in Western Blotting to investigate the effect of Sirt3 deficiency on venous thrombosis and NETosis in murine neutrophils and platelets.
Thu Jan 04 00:00:00 EST 2018
A Short Region of Connexin43 Reduces Human Glioma Stem Cell Migration, Invasion, and Survival through Src, PTEN, and FAK.
Stem cell reports
Jaraíz-Rodríguez M,Tabernero MD,González-Tablas M,Otero A,Orfao A,Medina JM,Tabernero A
AM4300 was used in Western Blotting to show that a cell-penetrating peptide inhibited c-Src and focal adhesion kinase, and upregulated phosphatase and tensin homolog in glioma stem cells derived from patients.
Tue Aug 08 00:00:00 EDT 2017
PJA2 ubiquitinates the HIV-1 Tat protein with atypical chain linkages to activate viral transcription.
Scientific reports
Faust TB,Li Y,Jang GM,Johnson JR,Yang S,Weiss A,Krogan NJ,Frankel AD
AM4300 was used in Western Blotting to describe a candidate RNAi screen of previously identified high-confidence Tat interacting proteins and report that several proteins from ubiquitin signalling pathways are essential for Tat activity.
Mon Mar 27 00:00:00 EDT 2017
Pervasive translational regulation of the cell signalling circuitry underlies mammalian development.
Nature communications
Fujii K,Shi Z,Zhulyn O,Denans N,Barna M
AM4300 was used in Western Blotting to monitor translation of the mammalian genome as cells become specified and organize into tissues in vivo.
Tue Feb 14 00:00:00 EST 2017
The neuroprotective agent Rasagiline mesylate attenuates cardiac remodeling after experimental myocardial infarction.
ESC heart failure
Varela A,Mavroidis M,Katsimpoulas M,Sfiroera I,Kappa N,Mesa A,Kostomitsopoulos NG,Cokkinos DV
AM4300 was used in Western Blotting to demonstrate a positive effect of rasagiline mesylate in the post-myocardial infarction period with a significant attenuation in cardiac remodelling.
Tue Aug 01 00:00:00 EDT 2017
Human cytomegalovirus-encoded viral cyclin-dependent kinase (v-CDK) UL97 phosphorylates and inactivates the retinoblastoma protein-related p107 and p130 proteins.
The Journal of biological chemistry
Iwahori S,Umaña AC,VanDeusen HR,Kalejta RF
AM4300 was used in Western Blotting to identify the retinoblastoma protein-related proteins, p107 and p130, as novel targets of human cytomegalovirus-encoded UL97.
Fri Apr 21 00:00:00 EDT 2017
Short-chain fatty acids and inulin, but not guar gum, prevent diet-induced obesity and insulin resistance through differential mechanisms in mice.
Scientific reports
Weitkunat K,Stuhlmann C,Postel A,Rumberger S,Fankhänel M,Woting A,Petzke KJ,Gohlke S,Schulz TJ,Blaut M,Klaus S,Schumann S
AM4300 was used in Western Blotting to investigate how various types of dietary fibre and different short-chain fatty acid ratios affect metabolic syndrome-related disorders.
Fri Jul 21 00:00:00 EDT 2017
Proteasome Activation by Small Molecules.
Cell chemical biology
Leestemaker Y,de Jong A,Witting KF,Penning R,Schuurman K,Rodenko B,Zaal EA,van de Kooij B,Laufer S,Heck AJR,Borst J,Scheper W,Berkers CR,Ovaa H
AM4300 was used in SDS-PAGE to study the activation of 26S proteasome activity and how this can be achieved through multiple mechanisms by distinct molecules.
Thu Jun 22 00:00:00 EDT 2017
Inhibition of IRE1α-mediated XBP1 mRNA cleavage by XBP1 reveals a novel regulatory process during the unfolded protein response.
Wellcome open research
Chalmers F,van Lith M,Sweeney B,Cain K,Bulleid NJ
AM4300 was used in Western Blotting to demonstrate that a feedback mechanism exists to attenuate inositol requiring enzyme α ribonuclease activity in the presence of X-box binding protein 1.
Sat Jan 29 00:00:00 EST 2022
Gα11 mutation in mice causes hypocalcemia rectifiable by calcilytic therapy.
JCI insight
Gorvin CM,Hannan FM,Howles SA,Babinsky VN,Piret SE,Rogers A,Freidin AJ,Stewart M,Paudyal A,Hough TA,Nesbit MA,Wells S,Vincent TL,Brown SD,Cox RD,Thakker RV
AM4300 was used in Western Blotting to establish Dsk7 mice, which harbor a germline gain-of-function Gα11 mutation, as a model for ADH2 and demonstrate calcilytics as a potential targeted therapy.
Thu Feb 09 00:00:00 EST 2017
Profiling Subcellular Protein Phosphatase Responses to Coxsackievirus B3 Infection of Cardiomyocytes.
Molecular & cellular proteomics : MCP
Shah M,Smolko CM,Kinicki S,Chapman ZD,Brautigan DL,Janes KA
AM4300 was used in Western Blotting to introduce a generalised functional assay for high-throughput profiling of multiple protein phosphatases.
Sat Apr 01 00:00:00 EDT 2017
A Highly Active Isoform of Lentivirus Restriction Factor SAMHD1 in Mouse.
The Journal of biological chemistry
Bloch N,Gläsker S,Sitaram P,Hofmann H,Shepard CN,Schultz ML,Kim B,Landau NR
AM4300 was used in Western Blotting to characterise the biochemical and antiviral properties of the two mouse isoforms of SAMHD1 (sterile α motif and histidine-aspartate domain-containing protein 1).
Fri Jan 20 00:00:00 EST 2017
Histone isoform H2A1H promotes attainment of distinct physiological states by altering chromatin dynamics.
Epigenetics & chromatin
Bhattacharya S,Reddy D,Jani V,Gadewal N,Shah S,Reddy R,Bose K,Sonavane U,Joshi R,Smoot D,Ashktorab H,Gupta S
AM4300 was used in Western Blotting to investigate the specific functional contribution of the histone H2A isoform H2A1H.
Wed Oct 18 00:00:00 EDT 2017
cFLIP critically modulates apoptotic resistance in epithelial-to-mesenchymal transition.
Oncotarget
Padmanabhan C,Rellinger EJ,Zhu J,An H,Woodbury LG,Chung DH,Waterson AG,Lindsley CW,Means AL,Beauchamp RD
AM4300 was used in Western Blotting to demonstrate that ML327 reverses much of the epithelial-to-mesenchymal transition gene expression programme in cultured carcinoma cell lines.
Fri Nov 24 00:00:00 EST 2017
Modulation of BCR Signaling by the Induced Dimerization of Receptor-Associated SYK.
Antibodies (Basel, Switzerland)
Westbroek ML,Geahlen RL
AM4300 was used in Western Blotting to explore factors that modulate the quantity and quality of signals sent by the crosslinked B cell antigen receptor (BCR), by developing a novel chemical mediator of dimerization to induce clustering of receptor-associated SYK.
Thu Dec 07 00:00:00 EST 2017
Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells.
Nature methods
Hess GT,Frésard L,Han K,Lee CH,Li A,Cimprich KA,Montgomery SB,Bassik MC
AM4300 was used in Western Blotting to develop CRISPR-X, a strategy to repurpose the somatic hypermutation machinery for protein engineering in situ.
Thu Dec 01 00:00:00 EST 2016
Macrophage PPARγ, a Lipid Activated Transcription Factor Controls the Growth Factor GDF3 and Skeletal Muscle Regeneration.
Immunity
Varga T,Mounier R,Patsalos A,Gogolák P,Peloquin M,Horvath A,Pap A,Daniel B,Nagy G,Pintye E,Póliska S,Cuvellier S,Larbi SB,Sansbury BE,Spite M,Brown CW,Chazaud B,Nagy L
AM4300 was used in Western Blotting to show that the lipid activated transcription factor, PPARγ, is required for proper skeletal muscle regeneration, acting in repair macrophages.
Tue Nov 15 00:00:00 EST 2016
A Novel Crizotinib-Resistant Solvent-Front Mutation Responsive to Cabozantinib Therapy in a Patient with ROS1-Rearranged Lung Cancer.
Clinical cancer research : an official journal of the American Association for Cancer Research
Drilon A,Somwar R,Wagner JP,Vellore NA,Eide CA,Zabriskie MS,Arcila ME,Hechtman JF,Wang L,Smith RS,Kris MG,Riely GJ,Druker BJ,O'Hare T,Ladanyi M,Davare MA
AM4300 was used in western blot to study a novel crizotinib-resistant solvent-front mutation in a patient with ROS1-rearranged lung cancer that is responsive to cabozantinib therapy
Sun May 15 00:00:00 EDT 2016
TRIM39 negatively regulates the NFκB-mediated signaling pathway through stabilization of Cactin.
Cellular and molecular life sciences : CMLS
Suzuki M,Watanabe M,Nakamaru Y,Takagi D,Takahashi H,Fukuda S,Hatakeyama S
AM4300 was used in western blot to assess negative regulation of the NF-kappaB-mediated signaling pathway through stabilization of Cactin by TRIM39
Tue Mar 01 00:00:00 EST 2016
Phosphorylation-dependent cleavage regulates von Hippel Lindau proteostasis and function.
Oncogene
German P,Bai S,Liu XD,Sun M,Zhou L,Kalra S,Zhang X,Minelli R,Scott KL,Mills GB,Jonasch E,Ding Z
AM4300 was used in western blot to determine the regulation of von Hippel Lindau proteostasis and function by phosphorylation-dependent cleavage
Thu Sep 22 00:00:00 EDT 2016
Knockout Mice Reveal a Major Role for Alveolar Epithelial Type I Cells in Alveolar Fluid Clearance.
American journal of respiratory cell and molecular biology
Flodby P,Kim YH,Beard LL,Gao D,Ji Y,Kage H,Liebler JM,Minoo P,Kim KJ,Borok Z,Crandall ED
AM4300 was used in western blot to determine the major role for alveolar epithelial type 1 cells in alveolar fluid clearance revealed by knockout mice
Thu Sep 01 00:00:00 EDT 2016
Reducing premature KCC2 expression rescues seizure susceptibility and spine morphology in atypical febrile seizures.
Neurobiology of disease
Awad PN,Sanon NT,Chattopadhyaya B,Carriço JN,Ouardouz M,Gagné J,Duss S,Wolf D,Desgent S,Cancedda L,Carmant L,Di Cristo G
AM4300 was used in western blot to investigate the rescue of seizure susceptibility and spine morphology in atypical febrile seizures by reducing premature KCC2 expression
Fri Jul 01 00:00:00 EDT 2016
Long and Short Isoforms of the Human Cytomegalovirus UL138 Protein Silence IE Transcription and Promote Latency.
Journal of virology
Lee SH,Caviness K,Albright ER,Lee JH,Gelbmann CB,Rak M,Goodrum F,Kalejta RF
AM4300 was used in Western Blotting to demonstrate that short and long isoforms of UL138 exist and can support latency, perhaps in temporally distinct manners.
Sat Oct 15 00:00:00 EDT 2016
Filamin-A is required to mediate SST2 effects in pancreatic neuroendocrine tumours.
Endocrine-related cancer
Vitali E,Cambiaghi V,Zerbi A,Carnaghi C,Colombo P,Peverelli E,Spada A,Mantovani G,Lania AG
AM4300 was used in Western Blotting to demonstrate that Filamin A plays a crucial role in somatostatin receptor type 2 expression, signalling, angiogenesis, cell adhesion and migration in pancreatic neuroendocrine tumours.
Tue Mar 01 00:00:00 EST 2016
Tbc1d1 deletion suppresses obesity in leptin-deficient mice.
International journal of obesity (2005)
Dokas J,Chadt A,Joost HG,Al-Hasani H
AM4300 was used in western blot to research suppression of obesity in leptin-deficient mice by Tbc1d1 deletion
Mon Aug 01 00:00:00 EDT 2016
Diacylglycerol pyrophosphate binds and inhibits the glyceraldehyde-3-phosphate dehydrogenase in barley aleurone.
Plant physiology and biochemistry : PPB
Astorquiza PL,Usorach J,Racagni G,Villasuso AL
AM4300 was used in western blot to characterize inhibition and binding of the glyceraldehyde-3-phosphate dehydrogenase in barley aleurone due to diacylglycerol pyrophosphate
Fri Apr 01 00:00:00 EDT 2016
Inositol-Triphosphate 3-Kinase C Mediates Inflammasome Activation and Treatment Response in Kawasaki Disease.
Journal of immunology (Baltimore, Md. : 1950)
Alphonse MP,Duong TT,Shumitzu C,Hoang TL,McCrindle BW,Franco A,Schurmans S,Philpott DJ,Hibberd ML,Burns J,Kuijpers TW,Yeung RS
AM4300 was used in western blot to discuss the contribution of IL-1beta and NLRP3 inflammasome activation to Kawasaki disease
Tue Nov 01 00:00:00 EDT 2016
A mutation abolishing the ZMPSTE24 cleavage site in prelamin A causes a progeroid disorder.
Journal of cell science
Wang Y,Lichter-Konecki U,Anyane-Yeboa K,Shaw JE,Lu JT,Östlund C,Shin JY,Clark LN,Gundersen GG,Nagy PL,Worman HJ
AM4300 was used in western blot to study the cause of progeroid disorder by a mutation abolishing the ZMPSTE24 cleavage site in prelamin A
Sun May 15 00:00:00 EDT 2016
ADAM10-Notch signaling governs the recruitment of ovarian pregranulosa cells and controls folliculogenesis in mice.
Journal of cell science
Feng L,Wang Y,Cai H,Sun G,Niu W,Xin Q,Tang X,Zhang J,Wang C,Zhang H,Xia G
AM4300 was used in western blot to assess the governing of the recruitment of ovarian pregranulosa cells and control of folliculogenesis in mice due to ADAM10-Notch signaling
Wed Jun 01 00:00:00 EDT 2016
Lung injury-induced skeletal muscle wasting in aged mice is linked to alterations in long chain fatty acid metabolism.
Metabolomics : Official journal of the Metabolomic Society
Files DC,Ilaiwy A,Parry TL,Gibbs KW,Liu C,Bain JR,Delbono O,Muehlbauer MJ,Willis MS
AM4300 was used in Western Blotting to develop a model of lung injury-induced muscle wasting in old mice and to evaluate the skeletal muscle metabolomic profile of adult and old acute lung injury mice.
Mon Aug 01 00:00:00 EDT 2016
The Neuregulin1/ErbB system is selectively regulated during peripheral nerve degeneration and regeneration.
The European journal of neuroscience
Ronchi G,Haastert-Talini K,Fornasari BE,Perroteau I,Geuna S,Gambarotta G
AM4300 was used in western blot to study the role of Neuregulin1/ErbB system during peripheral nerve degeneration and regeneration
Mon Feb 01 00:00:00 EST 2016
Sp1 downregulates proinflammatory cytokine‑induced catabolic gene expression in nucleus pulposus cells.
Molecular medicine reports
Xu K,Wang X,Zhang Q,Liang A,Zhu H,Huang D,Li C,Ye W
AM4300 was used in Western Blotting to examine the importance of specificity protein-1 in catabolic enzyme gene regulation during TNF-α stimulation.
Sat Oct 01 00:00:00 EDT 2016
Inhibition of the Biosynthesis of Prostaglandin E2 By Low-Dose Aspirin: Implications for Adenocarcinoma Metastasis.
Cancer prevention research (Philadelphia, Pa.)
Boutaud O,Sosa IR,Amin T,Oram D,Adler D,Hwang HS,Crews BC,Milne G,Harris BK,Hoeksema M,Knollmann BC,Lammers PE,Marnett LJ,Massion PP,Oates JA
AM4300 was used in Western Blotting to study whether low-dose aspirin inhibits prostaglandin E2 biosynthesis, and the implications this has in adenocarcinoma metastasis.
Tue Nov 01 00:00:00 EDT 2016
Novel subcellular localization of the DNA helicase Twinkle at the kinetochore complex during mitosis in neuronal-like progenitor cells.
Histochemistry and cell biology
Uittenbogaard M,Chiaramello A
AM4300 was used in Western Blotting to study the expression of DNA helicase, Twinkle, during chromosomal segregation at distinct mitotic phases.
Tue Mar 01 00:00:00 EST 2016
Synthesis and SAR assessment of novel Tubathian analogs in the pursuit of potent and selective HDAC6 inhibitors.
Organic & biomolecular chemistry
De Vreese R,Depetter Y,Verhaeghe T,Desmet T,Benoy V,Haeck W,Van Den Bosch L,D'hooghe M
AM4300 was used in Western Blotting to reveal hydroxamic acids can be recognized as lead structures for novel potent and selective HDAC6 inhibitors.
Sun Feb 28 00:00:00 EST 2016
The Oncoprotein BRD4-NUT Generates Aberrant Histone Modification Patterns.
PloS one
Zee BM,Dibona AB,Alekseyenko AA,French CA,Kuroda MI
AM4300 was used in Western Blotting to postulate that BRD4-NUT complexes override the preexisting histone code with new posttranslational modifications patterns that reflect aberrant transcription.
Tue Jun 27 00:00:00 EDT 2017
Pattern of RECK CpG methylation as a potential marker for predicting breast cancer prognosis and drug-sensitivity.
Oncotarget
Shi G,Yoshida Y,Yuki K,Nishimura T,Kawata Y,Kawashima M,Iwaisako K,Yoshikawa K,Kurebayashi J,Toi M,Noda M
AM4300 was used in Western Blotting to indicate the value of combined use of two RECK methylation markers for predicting prognosis and drug-sensitivity of breast cancers.
Tue Dec 13 00:00:00 EST 2016
Mitochondrial Ribosomal Protein L12 Is Required for POLRMT Stability and Exists as Two Forms Generated by Alternative Proteolysis during Import.
The Journal of biological chemistry
Nouws J,Goswami AV,Bestwick M,McCann BJ,Surovtseva YV,Shadel GS
AM4300 was used in western blot to determine the requirement of mitochondrial ribosomal protein L12 for POLRMT stability and exists as two forms generated by alternative proteolysis during import
Fri Jan 08 00:00:00 EST 2016
PIAS1 binds p300 and behaves as a coactivator or corepressor of the transcription factor c-Myb dependent on SUMO-status.
Biochimica et biophysica acta
Ledsaak M,Bengtsen M,Molværsmyr AK,Fuglerud BM,Matre V,Eskeland R,Gabrielsen OS
AM4300 was used in western blot to analyze the binding of p300 with PIAS1 acting as a coactivator or corepressor of the transcription factor c-Myb dependent on SUMO-status
Sun May 01 00:00:00 EDT 2016
Peroxisome Proliferator-activated Receptor-γ Activation Augments the β-Cell Unfolded Protein Response and Rescues Early Glycemic Deterioration and β Cell Death in Non-obese Diabetic Mice.
The Journal of biological chemistry
Maganti AV,Tersey SA,Syed F,Nelson JB,Colvin SC,Maier B,Mirmira RG
AM4300 was used in Western Blotting to demonstrate that PPAR-γ activation directly improves Β cell function and survival in non-obese diabetic mice.
Fri Oct 21 00:00:00 EDT 2016
Upregulation of the growth arrest-specific-2 in recurrent colorectal cancers, and its susceptibility to chemotherapy in a model cell system.
Biochimica et biophysica acta
Huang CJ,Lee CL,Yang SH,Chien CC,Huang CC,Yang RN,Chang CC
AM4300 was used in western blot to study growth arrest-specific-2 upregulation in recurrent colorectal cancer and its susceptibility to chemotherapy in a model cell system
Fri Jul 01 00:00:00 EDT 2016
Small Molecule Inhibitor of CBFβ-RUNX Binding for RUNX Transcription Factor Driven Cancers.
EBioMedicine
Illendula A,Gilmour J,Grembecka J,Tirumala VSS,Boulton A,Kuntimaddi A,Schmidt C,Wang L,Pulikkan JA,Zong H,Parlak M,Kuscu C,Pickin A,Zhou Y,Gao Y,Mishra L,Adli M,Castilla LH,Rajewski RA,Janes KA,Guzman ML,Bonifer C,Bushweller JH
AM4300 was used in Western Blotting to investigate whether targeting RUNX proteins could have broad utility in cancer therapeutics.
Wed Jun 01 00:00:00 EDT 2016
Commercially Available Preparations of Recombinant Wnt3a Contain Non-Wnt Related Activities Which May Activate TGF-β Signaling.
Journal of cellular biochemistry
Carthy JM,Engström U,Heldin CH,Moustakas A
AM4300 was used in Immunocytochemistry, Western Blot to find that treatment of cultured fibroblasts with recombinant Wnt3a induced immediate activation of TGF-β and BMP signaling and this activity appeared to be independent of the Wnt ligand itself.
Fri Apr 01 00:00:00 EDT 2016
Analysis of Epithelial-Mesenchymal Transition Induced by Transforming Growth Factor β.
Methods in molecular biology (Clifton, N.J.)
Valcourt U,Carthy J,Okita Y,Alcaraz L,Kato M,Thuault S,Bartholin L,Moustakas A
AM4300 was used in western blot to analyze the transition from epithelial-mesenchymal induced by transforming growth factor beta
Tue May 24 00:00:00 EDT 2016
Overexpression of the short endoglin isoform reduces renal fibrosis and inflammation after unilateral ureteral obstruction.
Biochimica et biophysica acta
Muñoz-Félix JM,Pérez-Roque L,Núñez-Gómez E,Oujo B,Arévalo M,Ruiz-Remolina L,Cuesta C,Langa C,Pérez-Barriocanal F,Bernabeu C,Lopez-Novoa JM
AM4300 was used in western blot to analyze the reduction of renal fibrosis and inflammation after unilateral ureteral obstruction due to overexpression of the short endoglin isoform
Thu Sep 01 00:00:00 EDT 2016
Disease Phenotypes in a Mouse Model of RNA Toxicity Are Independent of Protein Kinase Cα and Protein Kinase Cβ.
PloS one
Kim YK,Yadava RS,Mandal M,Mahadevan K,Yu Q,Leitges M,Mahadevan MS
AM4300 was used in western blot to clarify the role of protein kinase C in the pathogenesis of RNA toxicity
Wed Nov 20 00:00:00 EST 2019
Notch Signaling Activates Stem Cell Properties of Müller Glia through Transcriptional Regulation and Skp2-mediated Degradation of p27Kip1.
PloS one
Del Debbio CB,Mir Q,Parameswaran S,Mathews S,Xia X,Zheng L,Neville AJ,Ahmad I
AM4300 was used in western blot to study how transcriptional regulation and Skp2-mediated degradation of p27Kip1 activates stem cell properties of muller glia through notch signaling
Thu Aug 11 00:00:00 EDT 2016
The caffeine-binding adenosine A2A receptor induces age-like HPA-axis dysfunction by targeting glucocorticoid receptor function.
Scientific reports
Batalha VL,Ferreira DG,Coelho JE,Valadas JS,Gomes R,Temido-Ferreira M,Shmidt T,Baqi Y,Buée L,Müller CE,Hamdane M,Outeiro TF,Bader M,Meijsing SH,Sadri-Vakili G,Blum D,Lopes LV
AM4300 was used in western blot show that A2AR regulates GR function and contributes to age-related memory deficits
Thu Aug 11 00:00:00 EDT 2016
Mitochondrial DNA stress primes the antiviral innate immune response.
Nature
West AP,Khoury-Hanold W,Staron M,Tal MC,Pineda CM,Lang SM,Bestwick M,Duguay BA,Raimundo N,MacDuff DA,Kaech SM,Smiley JR,Means RE,Iwasaki A,Shadel GS
AM4300 was used in western blot to analyze the antiviral innate immune response due to mitochondrial DNA stress
Thu Apr 23 00:00:00 EDT 2015
Native elongating transcript sequencing reveals human transcriptional activity at nucleotide resolution.
Cell
Mayer A,di Iulio J,Maleri S,Eser U,Vierstra J,Reynolds A,Sandstrom R,Stamatoyannopoulos JA,Churchman LS
AM4300 was used in western blot to use native elongating transcript sequencing in human cells to globally map strand-specific RNA polymerase II density at nucleotide resolution
Thu Apr 23 00:00:00 EDT 2015
Hepatocyte Nicotinamide Adenine Dinucleotide Phosphate Reduced Oxidase 4 Regulates Stress Signaling, Fibrosis, and Insulin Sensitivity During Development of Steatohepatitis in Mice.
Gastroenterology
Bettaieb A,Jiang JX,Sasaki Y,Chao TI,Kiss Z,Chen X,Tian J,Katsuyama M,Yabe-Nishimura C,Xi Y,Szyndralewiez C,Schröder K,Shah A,Brandes RP,Haj FG,Török NJ
AM4300 was used in western blot to investigate the effects of nicotinamide adenine dinucleotide phosphate reduced oxidase 4 in liver tissues from patients with NASH and mice with steatohepatitis.
Sat Aug 01 00:00:00 EDT 2015
Dysregulated miRNA biogenesis downstream of cellular stress and ALS-causing mutations: a new mechanism for ALS.
The EMBO journal
Emde A,Eitan C,Liou LL,Libby RT,Rivkin N,Magen I,Reichenstein I,Oppenheim H,Eilam R,Silvestroni A,Alajajian B,Ben-Dov IZ,Aebischer J,Savidor A,Levin Y,Sons R,Hammond SM,Ravits JM,Möller T,Hornstein E
AM4300 was used in Western Blotting to show that extensive down-regulation of miRNA levels is a common molecular denominator for multiple forms of human amyotrophic lateral sclerosis.
Tue Nov 03 00:00:00 EST 2015
Structural insight into selectivity and resistance profiles of ROS1 tyrosine kinase inhibitors.
Proceedings of the National Academy of Sciences of the United States of America
Davare MA,Vellore NA,Wagner JP,Eide CA,Goodman JR,Drilon A,Deininger MW,O'Hare T,Druker BJ
AM4300 was used in western blot to discuss the sensitivity and selectivity of seven ROS and/or ALK inhibitors.
Tue Sep 29 00:00:00 EDT 2015
Rapamycin-mediated mTORC2 inhibition is determined by the relative expression of FK506-binding proteins.
Aging cell
Schreiber KH,Ortiz D,Academia EC,Anies AC,Liao CY,Kennedy BK
AM4300 was used in western blot to show that FKBP12 and FKBP51 levels determine the responsiveness of a cell line or tissue to rapamycin.
Wed Apr 01 00:00:00 EDT 2015
An analysis of critical factors for quantitative immunoblotting.
Science signaling
Janes KA
AM4300 was used in western blot to examine an immunoblot-analysis workflow for accuracy and precision
Tue Apr 07 00:00:00 EDT 2015
Loss of giant obscurins from breast epithelium promotes epithelial-to-mesenchymal transition, tumorigenicity and metastasis.
Oncogene
Shriver M,Stroka KM,Vitolo MI,Martin S,Huso DL,Konstantopoulos K,Kontrogianni-Konstantopoulos A
AM4300 was used in western blot to identify the role of obscurins during breast carcinogenesis
Thu Aug 06 00:00:00 EDT 2015
Transgenic expression of oncogenic BRAF induces loss of stem cells in the mouse intestine, which is antagonized by β-catenin activity.
Oncogene
Riemer P,Sreekumar A,Reinke S,Rad R,Schäfer R,Sers C,Bläker H,Herrmann BG,Morkel M
AM4300 was used in western blot to investigate how BRAF/MAPK activity regulates intestinal stem cell populations and contributes to colon cancer.
Thu Jun 11 00:00:00 EDT 2015
An siRNA Screen Identifies the U2 snRNP Spliceosome as a Host Restriction Factor for Recombinant Adeno-associated Viruses.
PLoS pathogens
Schreiber CA,Sakuma T,Izumiya Y,Holditch SJ,Hickey RD,Bressin RK,Basu U,Koide K,Asokan A,Ikeda Y
AM4300 was used in Western Blotting to identify host factors that might affect the key steps in adeno-associated virus infection.
Sat Aug 01 00:00:00 EDT 2015
Functional Analysis of the 60-Nucleotide Duplication in the Respiratory Syncytial Virus Buenos Aires Strain Attachment Glycoprotein.
Journal of virology
Hotard AL,Laikhter E,Brooks K,Hartert TV,Moore ML
AM4300 was used in Western Blotting to investigate whether the duplicated region in the glycoprotein gene (G) serves to enhance the function of G in the virus life cycle.
Sat Aug 01 00:00:00 EDT 2015
Identification of residues in the human respiratory syncytial virus fusion protein that modulate fusion activity and pathogenesis.
Journal of virology
Hotard AL,Lee S,Currier MG,Crowe JE,Sakamoto K,Newcomb DC,Peebles RS,Plemper RK,Moore ML
AM4300 was used in Western Blotting to establish the role of RSV F in virulence.
Thu Jan 01 00:00:00 EST 2015
Tamoxifen Inhibits TGF-β-Mediated Activation of Myofibroblasts by Blocking Non-Smad Signaling Through ERK1/2.
Journal of cellular physiology
Carthy JM,Sundqvist A,Heldin A,van Dam H,Kletsas D,Heldin CH,Moustakas A
AM4300 was used in western blot to show that tamoxifen prevents myofibroblast differentiation.
Tue Dec 01 00:00:00 EST 2015
MiR-497∼195 cluster microRNAs regulate osteoblast differentiation by targeting BMP signaling.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research
Grünhagen J,Bhushan R,Degenkolbe E,Jäger M,Knaus P,Mundlos S,Robinson PN,Ott CE
AM4300 was used in western blot to study microRNAs in bone development.
Fri May 01 00:00:00 EDT 2015
Parathyroid Hormone-Related Protein, Human Adipose-Derived Stem Cells Adipogenic Capacity and Healthy Obesity.
The Journal of clinical endocrinology and metabolism
Roca-Rodríguez MM,El Bekay R,Garrido-Sanchez L,Gómez-Serrano M,Coin-Aragüez L,Oliva-Olivera W,Lhamyani S,Clemente-Postigo M,García-Santos E,de Luna Diaz R,Yubero-Serrano EM,Fernández Real JM,Peral B,Tinahones FJ
AM4300 was used in western blot to test if PTHrP contributes to adipogenic regulation, obesity, and insulin resistance.
Mon Jun 01 00:00:00 EDT 2015
Parathyroid Hormone-Related Protein, Human Adipose-Derived Stem Cells Adipogenic Capacity and Healthy Obesity.
The Journal of clinical endocrinology and metabolism
Roca-Rodríguez MM,El Bekay R,Garrido-Sanchez L,Gómez-Serrano M,Coin-Aragüez L,Oliva-Olivera W,Lhamyani S,Clemente-Postigo M,García-Santos E,de Luna Diaz R,Yubero-Serrano EM,Fernández Real JM,Peral B,Tinahones FJ
AM4300 was used in western blot to test if PTHrP contributes to adipogenic regulation, obesity, and insulin resistance.
Mon Jun 01 00:00:00 EDT 2015
Inflammatory but not apoptotic death of granulocytes citrullinates fibrinogen.
Arthritis research & therapy
Blachère NE,Parveen S,Fak J,Frank MO,Orange DE
AM4300 was used in western blot to test if dying neutrophils release peptidyl arginine deiminase, which results in citrullination of antigens relevant to rheumatoid arthritis
Thu Dec 17 00:00:00 EST 2015
A Dual SILAC Proteomic Labeling Strategy for Quantifying Constitutive and Cell-Cell Induced Protein Secretion.
Journal of proteome research
Stiess M,Wegehingel S,Nguyen C,Nickel W,Bradke F,Cambridge SB
AM4300 was used in western blot to describe a strategy of dual SILAC labeling astrocytic cultures for in silico exclusion of unlabeled proteins from serum or neurons used for stimulation.
Fri Aug 07 00:00:00 EDT 2015
Missense variant in CCDC22 causes X-linked recessive intellectual disability with features of Ritscher-Schinzel/3C syndrome.
European journal of human genetics : EJHG
Kolanczyk M,Krawitz P,Hecht J,Hupalowska A,Miaczynska M,Marschner K,Schlack C,Emmerich D,Kobus K,Kornak U,Robinson PN,Plecko B,Grangl G,Uhrig S,Mundlos S,Horn D
AM4300 was used in western blot to identify a hypomorphic variant of CCDC22 in patients with RSS/3C syndrome in an Austrian family
Fri May 01 00:00:00 EDT 2015
The MAL protein is crucial for proper membrane condensation at the ciliary base, which is required for primary cilium elongation.
Journal of cell science
Reales E,Bernabé-Rubio M,Casares-Arias J,Rentero C,Fernández-Barrera J,Rangel L,Correas I,Enrich C,Andrés G,Alonso MA
AM4300 was used in immunocytochemistry to study the role of MAL, a tetraspanning protein, in primary cilium formation
Mon Jun 15 00:00:00 EDT 2015
Molecular Role of RNF43 in Canonical and Noncanonical Wnt Signaling.
Molecular and cellular biology
Tsukiyama T,Fukui A,Terai S,Fujioka Y,Shinada K,Takahashi H,Yamaguchi TP,Ohba Y,Hatakeyama S
AM4300 was used in Western blotting following immunoprecipitation to demonstrate that RNF43 suppresses both Wnt/Β-catenin signalling and noncanonical Wnt signalling by distinct mechanisms.
Mon Jun 01 00:00:00 EDT 2015
Molecular Role of RNF43 in Canonical and Noncanonical Wnt Signaling.
Molecular and cellular biology
Tsukiyama T,Fukui A,Terai S,Fujioka Y,Shinada K,Takahashi H,Yamaguchi TP,Ohba Y,Hatakeyama S
AM4300 was used in Western blotting following immunoprecipitation to demonstrate that RNF43 suppresses both Wnt/Β-catenin signalling and noncanonical Wnt signalling by distinct mechanisms.
Mon Jun 01 00:00:00 EDT 2015
Loss of auditory activity modifies the location of potassium channel KCNQ5 in auditory brainstem neurons.
Journal of neuroscience research
Caminos E,Garcia-Pino E,Juiz JM
AM4300 was used in western blot to analyze the modification of the location of potassium channel KCNQ5 in auditory brainstem neurons due to loss of auditory activity
Wed Apr 01 00:00:00 EDT 2015
Metformin increases peroxisome proliferator-activated receptor γ Co-activator-1α and utrophin a expression in dystrophic skeletal muscle.
Muscle & nerve
Ljubicic V,Jasmin BJ
AM4300 was used in western blot to identify the mechanism of metformin on dystrophic muscle
Wed Jul 01 00:00:00 EDT 2015
CD200: association with cancer stem cell features and response to chemoradiation in head and neck squamous cell carcinoma.
Head & neck
Jung YS,Vermeer PD,Vermeer DW,Lee SJ,Goh AR,Ahn HJ,Lee JH
AM4300 was used in western blot to correlate the expression of CD200 in various types of cancer with the responses to chemotherapy and radiation.
Sun Mar 01 00:00:00 EST 2015
Schizophrenia-like phenotype of polysialyltransferase ST8SIA2-deficient mice.
Brain structure & function
Kröcher T,Malinovskaja K,Jürgenson M,Aonurm-Helm A,Zharkovskaya T,Kalda A,Röckle I,Schiff M,Weinhold B,Gerardy-Schahn R,Hildebrandt H,Zharkovsky A
AM4300 was used in western blot to test if polyST deficiency results in a schizophrenia-like phenotype using knock out mice.
Thu Jan 01 00:00:00 EST 2015
Identification of intellectual disability genes showing circadian clock-dependent expression in the mouse hippocampus.
Neuroscience
Renaud J,Dumont F,Khelfaoui M,Foisset SR,Letourneur F,Bienvenu T,Khwaja O,Dorseuil O,Billuart P
AM4300 was used in western blot to study the rhythmic expression of intellectual disability genes in the mouse hippocampus
Thu Nov 12 00:00:00 EST 2015
FOXG1 expression shows correlation with neuronal differentiation in cerebellar development, aggressive phenotype in medulloblastomas, and survival in a xenograft model of medulloblastoma.
Human pathology
Adesina AM,Veo BL,Courteau G,Mehta V,Wu X,Pang K,Liu Z,Li XN,Peters L
AM4300 was used in western blot to investigate the role of FOXG1 in neuronal differentiation.
Tue Dec 01 00:00:00 EST 2015
CRISPR/Cas9: An inexpensive, efficient loss of function tool to screen human disease genes in Xenopus.
Developmental biology
Bhattacharya D,Marfo CA,Li D,Lane M,Khokha MK
AM4300 was used in Western Blotting to utilise CRISPR based F0 gene editing in Xenopus tropicalis for human congenital malformation candidate gene screening.
Tue Dec 15 00:00:00 EST 2015
Transcription factor Mohawk controls tenogenic differentiation of bone marrow mesenchymal stem cells in vitro and in vivo.
Journal of orthopaedic research : official publication of the Orthopaedic Research Society
Otabe K,Nakahara H,Hasegawa A,Matsukawa T,Ayabe F,Onizuka N,Inui M,Takada S,Ito Y,Sekiya I,Muneta T,Lotz M,Asahara H
AM4300 was used in western blot to determine the role of mohawk homeobox in ligament/tenogenic differentiation of bone marrow derived mesenchymal stem cells
Thu Jan 01 00:00:00 EST 2015
Filamin B Enhances the Invasiveness of Cancer Cells into 3D Collagen Matrices.
Cell structure and function
Iguchi Y,Ishihara S,Uchida Y,Tajima K,Mizutani T,Kawabata K,Haga H
AM4300 was used in western blot to study the contribution of filamin B on the invasiveness of cancer.
Mon Apr 04 00:00:00 EDT 2016
Analysis of human papillomavirus E7 protein status in C-33A cervical cancer cells.
Virus genes
Kaiser A,Jenewein B,Pircher H,Rostek U,Jansen-Dürr P,Zwerschke W
AM4300 was used in western blot to examine expression of the E7 protein in cervical cancer cell lines
Sun Feb 01 00:00:00 EST 2015
TRIM29 regulates the p63-mediated pathway in cervical cancer cells.
Biochimica et biophysica acta
Masuda Y,Takahashi H,Hatakeyama S
AM4300 was used in western blot to demonstrate that TRIM29 regulates the p63 pathway in cervical cancer cells
Thu Oct 01 00:00:00 EDT 2015
TRIM29 regulates the assembly of DNA repair proteins into damaged chromatin.
Nature communications
Masuda Y,Takahashi H,Sato S,Tomomori-Sato C,Saraf A,Washburn MP,Florens L,Conaway RC,Conaway JW,Hatakeyama S
AM4300 was used in western blot to elucidate the function of TRIM29 in double stranded break repair.
Mon Jun 22 00:00:00 EDT 2015
Syk Is Recruited to Stress Granules and Promotes Their Clearance through Autophagy.
The Journal of biological chemistry
Krisenko MO,Higgins RL,Ghosh S,Zhou Q,Trybula JS,Wang WH,Geahlen RL
AM4300 was used in western blot to show that Grb7 recruits Syk to the stress granule.
Fri Nov 13 00:00:00 EST 2015
MytiLec, a Mussel R-Type Lectin, Interacts with Surface Glycan Gb3 on Burkitt's Lymphoma Cells to Trigger Apoptosis through Multiple Pathways.
Marine drugs
Hasan I,Sugawara S,Fujii Y,Koide Y,Terada D,Iimura N,Fujiwara T,Takahashi KG,Kojima N,Rajia S,Kawsar SM,Kanaly RA,Uchiyama H,Hosono M,Ogawa Y,Fujita H,Hamako J,Matsui T,Ozeki Y
AM4300 was used in Western Blotting to indicate that the novel R-type lectin MytiLec initiates programmed cell death of Burkitt's lymphoma cells through multiple pathways based on interaction of the lectin with Gb3-containing glycosphingolipid-enriched microdomains on the cell surface.
Mon Dec 14 00:00:00 EST 2015
Aurora Kinase B Regulates Telomerase Activity via a Centromeric RNA in Stem Cells.
Cell reports
Mallm JP,Rippe K
AM4300 was used in Western Blotting to demonstrate in mouse embryonic stem cells, telomere maintenance is regulated via expression of cenRNA in a cell-cycle-dependent manner.
Tue Jun 16 00:00:00 EDT 2015
GAPDH and β-actin protein decreases with aging, making Stain-Free technology a superior loading control in Western blotting of human skeletal muscle.
Journal of applied physiology (Bethesda, Md. : 1985)
Vigelsø A,Dybboe R,Hansen CN,Dela F,Helge JW,Guadalupe Grau A
AM4300 was used in western blot to assess the best reference to use as a loading control for Western blotting of human skeletal muscle in applied physiology
Sun Feb 01 00:00:00 EST 2015
Integrity of the Pericentriolar Material Is Essential for Maintaining Centriole Association during M Phase.
PloS one
Seo MY,Jang W,Rhee K
AM4300 was used in western blot to study the role of the pericentriolar material disassembly in centriole separation during mitotic exit.
Fri Jun 17 00:00:00 EDT 2016
Genomic analysis of LPS-stimulated myeloid cells identifies a common pro-inflammatory response but divergent IL-10 anti-inflammatory responses.
Scientific reports
Hutchins AP,Takahashi Y,Miranda-Saavedra D
AM4300 was used in western blot to investigate the mechanisms downstream of STAT3 signaling that regulate inflammation
Fri Mar 13 00:00:00 EDT 2015
Antigen-B Cell Receptor Complexes Associate with Intracellular major histocompatibility complex (MHC) Class II Molecules.
The Journal of biological chemistry
Barroso M,Tucker H,Drake L,Nichol K,Drake JR
AM4300 was used in western blot to study molecular mechanisms used by B cells to control the source of peptides loaded onto class II molecules.
Fri Nov 06 00:00:00 EST 2015
Parkinson disease mutant E46K enhances α-synuclein phosphorylation in mammalian cell lines, in yeast, and in vivo.
The Journal of biological chemistry
Mbefo MK,Fares MB,Paleologou K,Oueslati A,Yin G,Tenreiro S,Pinto M,Outeiro T,Zweckstetter M,Masliah E,Lashuel HA
AM4300 was used in Western Blotting to investigate the effects of the A30P, E46K, and A53T Parkinson's disease-linked mutations on α-synuclein phosphorylation at residues Ser-87 and Ser-129.
Fri Apr 10 00:00:00 EDT 2015
Reduced keratin expression in colorectal neoplasia and associated fields is reversible by diet and resection.
BMJ open gastroenterology
Evans CA,Rosser R,Waby JS,Noirel J,Lai D,Wright PC,Williams EA,Riley SA,Bury JP,Corfe BM
AM4300 was used in western blot to identify molecular alterations in the normal mucosa in the proximity of adenomatous polyps and assess the modulating effect of butyrate.
Wed Oct 14 00:00:00 EDT 2015
Transcriptional activity of the islet β cell factor Pdx1 is augmented by lysine methylation catalyzed by the methyltransferase Set7/9.
The Journal of biological chemistry
Maganti AV,Maier B,Tersey SA,Sampley ML,Mosley AL,Özcan S,Pachaiyappan B,Woster PM,Hunter CS,Stein R,Mirmira RG
AM4300 was used in western blot to test if lys methylation of Pdx1 by Set7/9 affects Pdx1 transcriptional activity
Fri Apr 10 00:00:00 EDT 2015
Mitogen-Activated Protein Kinase 8 (MAP3K8) Mediates the Signaling Pathway of Estradiol Stimulating Progesterone Production Through G Protein-Coupled Receptor 30 (GPR30) in Mouse Corpus Luteum.
Molecular endocrinology (Baltimore, Md.)
Liu Y,Li Y,Zhang D,Liu J,Gou K,Cui S
AM4300 was used in western blot to elucidate the mechanism by which estradiol regulates progesterone production in the corpus luteum.
Fri May 01 00:00:00 EDT 2015
The metabolite α-ketoglutarate extends lifespan by inhibiting ATP synthase and TOR.
Nature
Chin RM,Fu X,Pai MY,Vergnes L,Hwang H,Deng G,Diep S,Lomenick B,Meli VS,Monsalve GC,Hu E,Whelan SA,Wang JX,Jung G,Solis GM,Fazlollahi F,Kaweeteerawat C,Quach A,Nili M,Krall AS,Godwin HA,Chang HR,Faull KF,Guo F,Jiang M,Trauger SA,Saghatelian A,Braas D,Christofk HR,Clarke CF,Teitell MA,Petrascheck M,Reue K,Jung ME,Frand AR,Huang J
AM4300 was used in Western Blotting to show that α-ketoglutarate, a tricarboxylic acid cycle intermediate, extends the lifespan of adult Caenorhabditis elegans.
Thu Jun 19 00:00:00 EDT 2014
Cyclin-dependent kinase 9 inhibition protects cartilage from the catabolic effects of proinflammatory cytokines.
Arthritis & rheumatology (Hoboken, N.J.)
Yik JH,Hu Z,Kumari R,Christiansen BA,Haudenschild DR
AM4300 was used in western blot to test if CDK-9 inhibition protects cartilage from the catabolic effects of proinflammatory cytokines.
Sun Jun 01 00:00:00 EDT 2014
IFNG-mediated immune responses enhance autophagy against Mycobacterium tuberculosis antigens in patients with active tuberculosis.
Autophagy
Rovetta AI,Peña D,Hernández Del Pino RE,Recalde GM,Pellegrini J,Bigi F,Musella RM,Palmero DJ,Gutierrez M,Colombo MI,García VE
AM4300 was used in western blot to study autophagy in Mycobacterium tuberculosis-infected patients.
Tue Dec 08 00:00:00 EST 2015
Genetic and pharmacological strategies to refunctionalize the von Hippel Lindau R167Q mutant protein.
Cancer research
Ding Z,German P,Bai S,Reddy AS,Liu XD,Sun M,Zhou L,Chen X,Zhao X,Wu C,Zhang S,Mills GB,Jonasch E
AM4300 was used in western blot to investigate how VHL-R67Q contributes to tumorigenesis.
Sun Jun 01 00:00:00 EDT 2014
Coordinate phosphorylation of multiple residues on single AKT1 and AKT2 molecules.
Oncogene
Guo H,Gao M,Lu Y,Liang J,Lorenzi PL,Bai S,Hawke DH,Li J,Dogruluk T,Scott KL,Jonasch E,Mills GB,Ding Z
AM4300 was used in western blot to study the phosphorylation of AKT and AKT2.
Thu Jun 26 00:00:00 EDT 2014
Cdk5-dependent Mst3 phosphorylation and activity regulate neuronal migration through RhoA inhibition.
The Journal of neuroscience : the official journal of the Society for Neuroscience
Tang J,Ip JP,Ye T,Ng YP,Yung WH,Wu Z,Fang W,Fu AK,Ip NY
AM4300 was used in Western Blotting to study the role of Mst3 in radial neuronal migration and final neuronal positioning in the developing mouse neocortex.
Wed May 28 00:00:00 EDT 2014
Regulation of ceramide synthase 6 in a spontaneous experimental autoimmune encephalomyelitis model is sex dependent.
Biochemical pharmacology
Eberle M,Ebel P,Wegner MS,Männich J,Tafferner N,Ferreiros N,Birod K,Schreiber Y,Krishnamoorthy G,Willecke K,Geisslinger G,Grösch S,Schiffmann S
AM4300 was used in western blot to study ceramide dysregulation in a chronic experimental autoimmune encephalomyelitis model
Sat Nov 15 00:00:00 EST 2014
SIRT1 inhibition during the hypoinflammatory phenotype of sepsis enhances immunity and improves outcome.
Journal of leukocyte biology
Vachharajani VT,Liu T,Brown CM,Wang X,Buechler NL,Wells JD,Yoza BK,McCall CE
AM4300 was used in western blot to investigate the effect of SIRT1 inhibition during sepsis
Sat Nov 01 00:00:00 EDT 2014
Soluble-E-cadherin activates HER and IAP family members in HER2+ and TNBC human breast cancers.
Molecular carcinogenesis
Brouxhon SM,Kyrkanides S,Teng X,O'Banion MK,Clarke R,Byers S,Ma L
AM4300 was used in western blot to study targeting sEcad for treatment of breast cancer.
Sat Nov 01 00:00:00 EDT 2014
Abnormal Ras signaling in Costello syndrome (CS) negatively regulates enamel formation.
Human molecular genetics
Goodwin AF,Tidyman WE,Jheon AH,Sharir A,Zheng X,Charles C,Fagin JA,McMahon M,Diekwisch TG,Ganss B,Rauen KA,Klein OD
AM4300 was used in western blot to demonstrate that Ras signaling is important for enamel formation in individuals with Costello syndrome and present mouse model system to dissect the roles of the Ras effector pathways in vivo.
Sat Feb 01 00:00:00 EST 2014
C9ORF72, implicated in amytrophic lateral sclerosis and frontotemporal dementia, regulates endosomal trafficking.
Human molecular genetics
Farg MA,Sundaramoorthy V,Sultana JM,Yang S,Atkinson RA,Levina V,Halloran MA,Gleeson PA,Blair IP,Soo KY,King AE,Atkin JD
AM4300 was used in western blot to show that C9ORF72 regulates endosomal trafficking.
Tue Jul 01 00:00:00 EDT 2014
Argininosuccinate lyase in enterocytes protects from development of necrotizing enterocolitis.
American journal of physiology. Gastrointestinal and liver physiology
Premkumar MH,Sule G,Nagamani SC,Chakkalakal S,Nordin A,Jain M,Ruan MZ,Bertin T,Dawson B,Zhang J,Schady D,Bryan NS,Campeau PM,Erez A,Lee B
AM4300 was used in western blot to confirm the role of argininosuccinate lyase deficiency from enterocytes in the pathogenesis of necrotizing enterocolitis
Fri Aug 01 00:00:00 EDT 2014
Pax6 is essential for the maintenance and multi-lineage differentiation of neural stem cells, and for neuronal incorporation into the adult olfactory bulb.
Stem cells and development
Curto GG,Nieto-Estévez V,Hurtado-Chong A,Valero J,Gómez C,Alonso JR,Weruaga E,Vicario-Abejón C
AM4300 was used in western blot to study the role of Pax6 in the maintenance and differentiation of adult neural stem cells and in adult neurogenesis
Mon Dec 01 00:00:00 EST 2014
Lack of effect of a P2Y6 receptor antagonist on neuropathic pain behavior in mice.
Pharmacology, biochemistry, and behavior
Syhr KM,Kallenborn-Gerhardt W,Lu R,Olbrich K,Schmitz K,Männich J,Ferreiros-Bouzas N,Geisslinger G,Niederberger E,Schmidtko A
AM4300 was used in western blot to examine the role of P2Y6 receptors in pain processing
Mon Sep 01 00:00:00 EDT 2014
St8sia2 deficiency plus juvenile cannabis exposure in mice synergistically affect higher cognition in adulthood.
Behavioural brain research
Tantra M,Kröcher T,Papiol S,Winkler D,Röckle I,Jatho J,Burkhardt H,Ronnenberg A,Gerardy-Schahn R,Ehrenreich H,Hildebrandt H
AM4300 was used in western blot to study delta-9-tetrahydrocannabinol in St8sia2(-/-) mice
Mon Dec 15 00:00:00 EST 2014
Hydrostatic pressure enhances mitomycin C induced apoptosis in urothelial carcinoma cells.
Urologic oncology
Chen SK,Chung CA,Cheng YC,Huang CJ,Ruaan RC,Chen WY,Li C,Tsao CW,Hu WW,Chien CC
AM4300 was used in western blot to study the mechanism of the mitomycin C on urothelial carcinoma cells
Wed Jan 01 00:00:00 EST 2014
Protein arginine methyltransferase 6 enhances ligand-dependent and -independent activity of estrogen receptor α via distinct mechanisms.
Biochimica et biophysica acta
Sun Y,Chung HH,Woo AR,Lin VC
AM4300 was used in western blot to investigate how PRMT6 promotes ERα activity.
Mon Sep 01 00:00:00 EDT 2014
Role of D-Limonene in autophagy induced by bergamot essential oil in SH-SY5Y neuroblastoma cells.
PloS one
Russo R,Cassiano MG,Ciociaro A,Adornetto A,Varano GP,Chiappini C,Berliocchi L,Tassorelli C,Bagetta G,Corasaniti MT
AM4300 was used in Western Blotting to study the ability of bergamot essential oil to modulate autophagic pathways in human SH-SY5Y neuroblastoma cells.
Tue Jan 26 00:00:00 EST 2016
miR-142 orchestrates a network of actin cytoskeleton regulators during megakaryopoiesis.
eLife
Chapnik E,Rivkin N,Mildner A,Beck G,Pasvolsky R,Metzl-Raz E,Birger Y,Amir G,Tirosh I,Porat Z,Israel LL,Lellouche E,Michaeli S,Lellouche JP,Izraeli S,Jung S,Hornstein E
AM4300 was used in western blot to determine the in vivo functions of miR-142
Fri May 23 00:00:00 EDT 2014
Prevention of intestinal obstruction reveals progressive neurodegeneration in mutant TDP-43 (A315T) mice.
Molecular neurodegeneration
Herdewyn S,Cirillo C,Van Den Bosch L,Robberecht W,Vanden Berghe P,Van Damme P
AM4300 was used in Western Blotting to understand and overcome the cause of sudden death in TDP-43 (A315T) mice.
Tue Jun 17 00:00:00 EDT 2014
Norovirus translation requires an interaction between the C Terminus of the genome-linked viral protein VPg and eukaryotic translation initiation factor 4G.
The Journal of biological chemistry
Chung L,Bailey D,Leen EN,Emmott EP,Chaudhry Y,Roberts LO,Curry S,Locker N,Goodfellow IG
AM4300 was used in western blot to identify the components of the norovirus translation initiation factor complex
Fri Aug 01 00:00:00 EDT 2014
Serum stability and physicochemical characterization of a novel amphipathic peptide C6M1 for siRNA delivery.
PloS one
Jafari M,Xu W,Pan R,Sweeting CM,Karunaratne DN,Chen P
AM4300 was used in western blot to investigate the use of peptides as carriers of short interfering RNA.
Mon Jun 15 00:00:00 EDT 2015
PINK1 deficiency sustains cell proliferation by reprogramming glucose metabolism through HIF1.
Nature communications
Requejo-Aguilar R,Lopez-Fabuel I,Fernandez E,Martins LM,Almeida A,Bolaños JP
AM4300 was used in western blot to show that that PINK1 deficiency triggers hypoxia-inducible factor-1α.
Thu Jul 24 00:00:00 EDT 2014
A p38MAPK/MK2 signaling pathway leading to redox stress, cell death and ischemia/reperfusion injury.
Cell communication and signaling : CCS
Ashraf MI,Ebner M,Wallner C,Haller M,Khalid S,Schwelberger H,Koziel K,Enthammer M,Hermann M,Sickinger S,Soleiman A,Steger C,Vallant S,Sucher R,Brandacher G,Santer P,Dragun D,Troppmair J
AM4300 was used in western blot to demonstrate that p38MAPK activation elevates mitochondrial ROS levels.
Tue Jan 14 00:00:00 EST 2014
CD137 enhancement of HPV positive head and neck squamous cell carcinoma tumor clearance.
Vaccines
Lucido CT,Vermeer PD,Wieking BG,Vermeer DW,Lee JH
AM4300 was used in western blot to study the effect of CD137 on HPV positive head and neck squamous cell carcinoma tumor clearance
Tue Sep 08 00:00:00 EDT 2015
Release of tensile strain on engineered human tendon tissue disturbs cell adhesions, changes matrix architecture, and induces an inflammatory phenotype.
PloS one
Bayer ML,Schjerling P,Herchenhan A,Zeltz C,Heinemeier KM,Christensen L,Krogsgaard M,Gullberg D,Kjaer M
AM4300 was used in western blot to characterize engineered human tendon tissue and how release of tensile strain changes matrix architecture, disturbs cell adhesions, and induces an inflammatory phenotype
Wed Oct 29 00:00:00 EDT 2014
Differential glial activation during the degeneration of Purkinje cells and mitral cells in the PCD mutant mice.
Glia
Baltanás FC,Berciano MT,Valero J,Gómez C,Díaz D,Alonso JR,Lafarga M,Weruaga E
AM4300 was used in western blot to study gliosis during Purkinje and mitral cell death in the Purkinje Cell Degeneration mouse.
Fri Feb 01 00:00:00 EST 2013
Contrasting roles of mitogen-activated protein kinases in cellular entry and replication of hepatitis C virus: MKNK1 facilitates cell entry.
Journal of virology
Kim S,Ishida H,Yamane D,Yi M,Swinney DC,Foung S,Lemon SM
AM4300 was used in western blot to study the roles of MAPK-related kinase and MKNK-1 in HCV replication and cellular entry.
Mon Apr 01 00:00:00 EDT 2013
Isolation of a novel thioflavin S-derived compound that inhibits BAG-1-mediated protein interactions and targets BRAF inhibitor-resistant cell lines.
Molecular cancer therapeutics
Enthammer M,Papadakis ES,Salomé Gachet M,Deutsch M,Schwaiger S,Koziel K,Ashraf MI,Khalid S,Wolber G,Packham G,Cutress RI,Stuppner H,Troppmair J
AM4300 was used in Western Blotting to report the development of a protocol for the purification and isolation of biologically active constituents of Thioflavin S and the characterization of the novel compound Thio-2.
Fri Nov 01 00:00:00 EDT 2013
Cellular and extracellular matrix changes in anterior cruciate ligaments during human knee aging and osteoarthritis.
Arthritis research & therapy
Hasegawa A,Nakahara H,Kinoshita M,Asahara H,Koziol J,Lotz MK
AM4300 was used in Western Blotting to characterise the patterns and relations of ageing-related and osteoarthritis-associated changes in anterior cruciate ligament cells and the extracellular matrix.
Thu Feb 14 00:00:00 EST 2013
Impaired surface expression and conductance of the KCNQ4 channel lead to sensorineural hearing loss.
Journal of cellular and molecular medicine
Gao Y,Yechikov S,Vázquez AE,Chen D,Nie L
AM4300 was used in Western Blotting to study the molecular aetiology of DFNA2.
Mon Jul 01 00:00:00 EDT 2013
Tumor protein D52 represents a negative regulator of ATM protein levels.
Cell cycle (Georgetown, Tex.)
Chen Y,Kamili A,Hardy JR,Groblewski GE,Khanna KK,Byrne JA
AM4300 was used in western blot to study the relationship between tumor protein D52 and ATM protein
Sun Sep 15 00:00:00 EDT 2013
Wild-type and mutant p53 mediate cisplatin resistance through interaction and inhibition of active caspase-9.
Cell cycle (Georgetown, Tex.)
Chee JL,Saidin S,Lane DP,Leong SM,Noll JE,Neilsen PM,Phua YT,Gabra H,Lim TM
AM4300 was used in Western Blotting to suggest the inhibition of caspase-9 as a mechanism in evading apoptosis in tumours with high-level cytoplasmically localized p53 expression.
Tue Jan 15 00:00:00 EST 2013
Human cytomegalovirus chemokine receptor US28 induces migration of cells on a CX3CL1-presenting surface.
The Journal of general virology
Hjortø GM,Kiilerich-Pedersen K,Selmeczi D,Kledal TN,Larsen NB
AM4300 was used in Western Blotting to indicate that infected cells probably move more than uninfected cells in inflamed tissues with high CX3CL1 expression, with soluble chemokines affecting the final migration.
Wed May 01 00:00:00 EDT 2013
Noncanonical NOTCH signaling limits self-renewal of human epithelial and induced pluripotent stem cells through ROCK activation.
Molecular and cellular biology
Yugawa T,Nishino K,Ohno S,Nakahara T,Fujita M,Goshima N,Umezawa A,Kiyono T
AM4300 was used in Western Blotting to demonstrate that the NOTCH-ROCK pathway is critical for cellular differentiation, and loss of self-renewal capacity in a subset of immature cells.
Fri Nov 01 00:00:00 EDT 2013
Conventional knockout of Tbc1d1 in mice impairs insulin- and AICAR-stimulated glucose uptake in skeletal muscle.
Endocrinology
Dokas J,Chadt A,Nolden T,Himmelbauer H,Zierath JR,Joost HG,Al-Hasani H
AM4300 was used in western blot to investigate the role of Tbc1d1 in insulin- and AICAR-stimulated glucose uptake in skeletal muscle
Tue Oct 01 00:00:00 EDT 2013
Cycloheximide inhibits follicle-stimulating hormone β subunit transcription by blocking de novo synthesis of the labile activin type II receptor in gonadotrope cells.
Cellular signalling
Rejon CA,Ho CC,Wang Y,Zhou X,Bernard DJ,Hébert TE
AM4300 was used in western blot to demonstrate that cycloheximide produces inhibin-like effects in gonadotropes by preventing de novo synthesis of ACVR2.
Sat Jun 01 00:00:00 EDT 2013
Persistent fibrosis in the liver of choline-deficient and iron-supplemented L-amino acid-defined diet-induced nonalcoholic steatohepatitis rat due to continuing oxidative stress after choline supplementation.
Toxicology and applied pharmacology
Takeuchi-Yorimoto A,Noto T,Yamada A,Miyamae Y,Oishi Y,Matsumoto M
AM4300 was used in western blot to study nonalcoholic steatohepatitis using rats.
Wed May 01 00:00:00 EDT 2013
Estrogen responsiveness of the TFIID subunit TAF4B in the normal mouse ovary and in ovarian tumors.
Biology of reproduction
Wardell JR,Hodgkinson KM,Binder AK,Seymour KA,Korach KS,Vanderhyden BC,Freiman RN
AM4300 was used in Western Blotting to elucidate the relationship between oestrogen signalling and the TAF4B subunit in normal or tumourigenic ovaries.
Fri Nov 01 00:00:00 EDT 2013
N-cadherin expression is regulated by UTP in schwannoma cells.
Purinergic signalling
Martiáñez T,Lamarca A,Casals N,Gella A
AM4300 was used in western blot to examine the role of UTP on N-cadherin expression in schwannoma cells
Sat Jun 01 00:00:00 EDT 2013
In vivo analysis of kallikrein-related peptidase 6 (KLK6) function in oligodendrocyte development and the expression of myelin proteins.
Neuroscience
Murakami K,Jiang YP,Tanaka T,Bando Y,Mitrovic B,Yoshida S
AM4300 was used in western blot to investigate the functions of KLK6 in oligodendrocyte lineage cell development and myelin protein production
Tue Apr 16 00:00:00 EDT 2013
Interaction of PICK1 with C-terminus of growth hormone-releasing hormone receptor (GHRHR) modulates trafficking and signal transduction of human GHRHR.
Journal of pharmacological sciences
Katsushima Y,Sato T,Yamada C,Ito M,Suzuki Y,Ogawa E,Sukegawa I,Sukegawa J,Fukunaga K,Yanagisawa T
AM4300 was used in western blot to identify protein interacting with C-kinase as a binding partner of growth hormone-releasing hormone receptor.
Tue Apr 01 00:00:00 EDT 2014
Mitochondrial DNA (mtDNA) in brain samples from patients with major psychiatric disorders: gene expression profiles, mtDNA content and presence of the mtDNA common deletion.
American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics
Torrell H,Montaña E,Abasolo N,Roig B,Gaviria AM,Vilella E,Martorell L
AM4300 was used in western blot to determine if mitochondrial DNA expression or content contribute to the mitochondrial dysfunction observed in schizophrenia, bipolar disorder, and major depressive disorder.
Fri Mar 01 00:00:00 EST 2013
SnoN/SKIL modulates proliferation through control of hsa-miR-720 transcription in esophageal cancer cells.
Biochemical and biophysical research communications
Shinozuka E,Miyashita M,Mizuguchi Y,Akagi I,Kikuchi K,Makino H,Matsutani T,Hagiwara N,Nomura T,Uchida E,Takizawa T
AM4300 was used in western blot to study mechanisms of transcriptional regulation of miRNAs using esophageal squamous cell carcinoma.
Fri Jan 04 00:00:00 EST 2013
Surface proteins of C6/36 cells involved in dengue virus 4 binding and entry.
Archives of virology
Vega-Almeida TO,Salas-Benito M,De Nova-Ocampo MA,Del Angel RM,Salas-Benito JS
AM4300 was used in western blot to identify host proteins involved in Dengue virus cell entry.
Sat Jun 01 00:00:00 EDT 2013
Distinct roles of molecular chaperones HSP90α and HSP90β in the biogenesis of KCNQ4 channels.
PloS one
Gao Y,Yechikov S,Vazquez AE,Chen D,Nie L
AM4300 was used in western blot to examine the role of heat shock proteins in the biogenesis of KCNQ4 channels
Thu Aug 15 00:00:00 EDT 2013
α-1,6-Fucosyltransferase (FUT8) inhibits hemoglobin production during differentiation of murine and K562 human erythroleukemia cells.
The Journal of biological chemistry
Sasaki H,Toda T,Furukawa T,Mawatari Y,Takaesu R,Shimizu M,Wada R,Kato D,Utsugi T,Ohtsu M,Murakami Y
AM4300 was used in Western Blotting to define FUT8 as a novel factor for haemoglobin production and demonstrate that core fucosylation plays an important role in erythroid differentiation.
Fri Jun 07 00:00:00 EDT 2013
α-1,6-Fucosyltransferase (FUT8) inhibits hemoglobin production during differentiation of murine and K562 human erythroleukemia cells.
The Journal of biological chemistry
Sasaki H,Toda T,Furukawa T,Mawatari Y,Takaesu R,Shimizu M,Wada R,Kato D,Utsugi T,Ohtsu M,Murakami Y
AM4300 was used in Western Blotting to define FUT8 as a novel factor for haemoglobin production and demonstrate that core fucosylation plays an important role in erythroid differentiation.
Fri Jun 07 00:00:00 EDT 2013
Identification and characterization of a novel human methyltransferase modulating Hsp70 protein function through lysine methylation.
The Journal of biological chemistry
Jakobsson ME,Moen A,Bousset L,Egge-Jacobsen W,Kernstock S,Melki R,Falnes PØ
AM4300 was used in western blot to investigate the mechanism and function of trimethylated HSPA8.
Fri Sep 27 00:00:00 EDT 2013
A novel role for junctional adhesion molecule-A in tumor proliferation: modulation by an anti-JAM-A monoclonal antibody.
International journal of cancer
Goetsch L,Haeuw JF,Beau-Larvor C,Gonzalez A,Zanna L,Malissard M,Lepecquet AM,Robert A,Bailly C,Broussas M,Corvaia N
AM4300 was used in Western Blotting to show for the first time that JAM-A can interfere with tumor proliferation and suggest that JAM-A is a potential novel target in oncology.
Fri Mar 15 00:00:00 EDT 2013
Cartilage oligomeric matrix protein enhances osteogenesis by directly binding and activating bone morphogenetic protein-2.
Bone
Ishida K,Acharya C,Christiansen BA,Yik JH,DiCesare PE,Haudenschild DR
AM4300 was used in western blot to test if COMP binds to BMP-2 and test if COMP promotes the biological activity of BMP-2 with respect to osteogenesis.
Mon Jul 01 00:00:00 EDT 2013
A role for mDia, a Rho-regulated actin nucleator, in tangential migration of interneuron precursors.
Nature neuroscience
Shinohara R,Thumkeo D,Kamijo H,Kaneko N,Sawamoto K,Watanabe K,Takebayashi H,Kiyonari H,Ishizaki T,Furuyashiki T,Narumiya S
AM4300 was used in western blot to investigate factors that regulate excitatory and inhibitory neuron migration.
Sun Jan 15 00:00:00 EST 2012
Genome-wide analysis of STAT3 binding in vivo predicts effectors of the anti-inflammatory response in macrophages.
Blood
Hutchins AP,Poulain S,Miranda-Saavedra D
AM4300 was used in western blot to identify STAT3-controlled effectors of the anti-inflammatory response.
Thu Mar 29 00:00:00 EDT 2012
GRB2 interaction with the ecotropic murine leukemia virus receptor, mCAT-1, controls virus entry and is stimulated by virus binding.
Journal of virology
Chen Z,Kolokoltsov AA,Wang J,Adhikary S,Lorinczi M,Elferink LA,Davey RA
AM4300 was used in knockdown western blot and western blot to test if GRB2 contributes to controlling infection by retroviruses by affecting receptor function.
Wed Feb 01 00:00:00 EST 2012
Nestin expression is lost in ventricular fibroblasts during postnatal development of the rat heart and re-expressed in scar myofibroblasts.
Journal of cellular physiology
Béguin PC,Gosselin H,Mamarbachi M,Calderone A
AM4300 was used in western blot to examine nestin expression in ventricular fibroblasts.
Wed Feb 01 00:00:00 EST 2012
Abnormal platelet aggregation in idiopathic pulmonary arterial hypertension: role of nitric oxide.
American journal of physiology. Lung cellular and molecular physiology
Aytekin M,Aulak KS,Haserodt S,Chakravarti R,Cody J,Minai OA,Dweik RA
AM4300 was used in western blot to determine the phenotype of the platelets in patients with idiopathic pulmonary arterial hypertension.
Thu Mar 15 00:00:00 EDT 2012
The Rab-GTPase-activating protein TBC1D1 regulates skeletal muscle glucose metabolism.
American journal of physiology. Endocrinology and metabolism
Szekeres F,Chadt A,Tom RZ,Deshmukh AS,Chibalin AV,Björnholm M,Al-Hasani H,Zierath JR
AM4300 was used in Western Blotting to demonstrate that TBC1D1 plays a role in regulation of glucose metabolism in skeletal muscle.
Wed Aug 15 00:00:00 EDT 2012
High basal γH2AX levels sustain self-renewal of mouse embryonic and induced pluripotent stem cells.
Stem cells (Dayton, Ohio)
Turinetto V,Orlando L,Sanchez-Ripoll Y,Kumpfmueller B,Storm MP,Porcedda P,Minieri V,Saviozzi S,Accomasso L,Cibrario Rocchietti E,Moorwood K,Circosta P,Cignetti A,Welham MJ,Giachino C
AM4300 was used in western blot to test if H2AX phosphorylation is important in maintaining self-renewal of mouse embryonic stem cells.
Sun Jul 01 00:00:00 EDT 2012
Role of caspases in cytokine-induced barrier breakdown in human brain endothelial cells.
Journal of immunology (Baltimore, Md. : 1950)
Lopez-Ramirez MA,Fischer R,Torres-Badillo CC,Davies HA,Logan K,Pfizenmaier K,Male DK,Sharrack B,Romero IA
AM4300 was used in western blot to study the role of caspases in cytokine-induced barrier breakdown during neuroinflammation
Sat Sep 15 00:00:00 EDT 2012
Efficient synaptic vesicle recycling after intense exocytosis concomitant with the accumulation of non-releasable endosomes at early developmental stages.
Journal of cell science
Bartolomé-Martín D,Ramírez-Franco J,Castro E,Sánchez-Prieto J,Torres M
AM4300 was used in Western Blotting to determine whether nerve terminals use multiple pathways of endocytosis to recycle synaptic vesicles.
Sun Jan 15 00:00:00 EST 2012
Fragile X premutation RNA is sufficient to cause primary ovarian insufficiency in mice.
Human molecular genetics
Lu C,Lin L,Tan H,Wu H,Sherman SL,Gao F,Jin P,Chen D
AM4300 was used in western blot to characterize mice carrying the human FMR premutation allele.
Sat Dec 01 00:00:00 EST 2012
The imprinted H19 gene regulates human placental trophoblast cell proliferation via encoding miR-675 that targets Nodal Modulator 1 (NOMO1).
RNA biology
Gao WL,Liu M,Yang Y,Yang H,Liao Q,Bai Y,Li YX,Li D,Peng C,Wang YL
AM4300 was used in western blot to identify the roles of H9 gene via the miR-675 pathway in the pathogenesis of preeclampsia.
Sun Jul 01 00:00:00 EDT 2012
Distinct immunomodulatory effects of a panel of nanomaterials in human dermal fibroblasts.
Toxicology letters
Romoser AA,Figueroa DE,Sooresh A,Scribner K,Chen PL,Porter W,Criscitiello MF,Sayes CM
AM4300 was used in western blot to assess the effects of nanoparticles on inflammation and cellular stress.
Sat May 05 00:00:00 EDT 2012
Changes in the serotonergic system and in brain-derived neurotrophic factor distribution in the main olfactory bulb of pcd mice before and after mitral cell loss.
Neuroscience
Gómez C,Curto GG,Baltanás FC,Valero J,O'Shea E,Colado MI,Díaz D,Weruaga E,Alonso JR
AM4300 was used in western blot to study the response of the serotonergic centrifugal system after mitral cell loss using Purkinje cell degeneration mutant mice.
Tue Jan 10 00:00:00 EST 2012
The estrogen-responsive Agr2 gene regulates mammary epithelial proliferation and facilitates lobuloalveolar development.
Developmental biology
Verma S,Salmans ML,Geyfman M,Wang H,Yu Z,Lu Z,Zhao F,Lipkin SM,Andersen B
AM4300 was used as a Control in experiments to define the role of Agr2 in normal mammary gland development using Agr2 knockout and conditional overexpression mouse models.
Sat Sep 15 00:00:00 EDT 2012
The estrogen-responsive Agr2 gene regulates mammary epithelial proliferation and facilitates lobuloalveolar development.
Developmental biology
Verma S,Salmans ML,Geyfman M,Wang H,Yu Z,Lu Z,Zhao F,Lipkin SM,Andersen B
AM4300 was used as a Control in experiments to define the role of Agr2 in normal mammary gland development using Agr2 knockout and conditional overexpression mouse models.
Sat Sep 15 00:00:00 EDT 2012
The transformation suppressor gene Reck is required for postaxial patterning in mouse forelimbs.
Biology open
Yamamoto M,Matsuzaki T,Takahashi R,Adachi E,Maeda Y,Yamaguchi S,Kitayama H,Echizenya M,Morioka Y,Alexander DB,Yagi T,Itohara S,Nakamura T,Akiyama H,Noda M
AM4300 was used in western blot to characterize mice with reduced Reck-expression
Tue May 15 00:00:00 EDT 2012
Mouse germ cell clusters form by aggregation as well as clonal divisions.
Mechanisms of development
Mork L,Tang H,Batchvarov I,Capel B
AM4300 was used in immunohistochemistry to test if germ cell clusters in the mammalian gonad arise through incomplete cell divisions.
Thu Jul 05 00:00:00 EDT 2012
Agents that stabilize mutated von Hippel-Lindau (VHL) protein: results of a high-throughput screen to identify compounds that modulate VHL proteostasis.
Journal of biomolecular screening
Ding Z,German P,Bai S,Feng Z,Gao M,Si W,Sobieski MM,Stephan CC,Mills GB,Jonasch E
AM4300 was used in Western Blotting to develop a cell-based screening tool to identify compounds that stabilize or upregulate full-length, point-mutated Von Hippel-Lindau protein.
Fri Jun 01 00:00:00 EDT 2012
Profiling of linker histone variants in ovarian cancer.
Frontiers in bioscience (Landmark edition)
Medrzycki M,Zhang Y,McDonald JF,Fan Y
AM4300 was used in western blot to elucidate the impact of H in ovarian cancer.
Sun Jan 01 00:00:00 EST 2012
Uncovering suitable reference proteins for expression studies in human adipose tissue with relevance to obesity.
PloS one
Pérez-Pérez R,López JA,García-Santos E,Camafeita E,Gómez-Serrano M,Ortega-Delgado FJ,Ricart W,Fernández-Real JM,Peral B
AM4300 was used in western blot to identify and validate reference proteins for data standardization.
Mon Jun 11 00:00:00 EDT 2012
Mitochondrial physiology and gene expression analyses reveal metabolic and translational dysregulation in oocyte-induced somatic nuclear reprogramming.
PloS one
Esteves TC,Psathaki OE,Pfeiffer MJ,Balbach ST,Zeuschner D,Shitara H,Yonekawa H,Siatkowski M,Fuellen G,Boiani M
AM4300 was used in immunocytochemistry to develop and use methods to directly assess maternal and embryonic products.
Tue Oct 02 00:00:00 EDT 2012
Separase-dependent cleavage of pericentrin B is necessary and sufficient for centriole disengagement during mitosis.
Cell cycle (Georgetown, Tex.)
Lee K,Rhee K
AM4300 was used in Western Blotting to characterise the specific cleavage of pericentrin B by separase at the exit of mitosis.
Sun Jul 01 00:00:00 EDT 2012
Transcriptional induction of salt-inducible kinase 1 by transforming growth factor β leads to negative regulation of type I receptor signaling in cooperation with the Smurf2 ubiquitin ligase.
The Journal of biological chemistry
Lönn P,Vanlandewijck M,Raja E,Kowanetz M,Watanabe Y,Kowanetz K,Vasilaki E,Heldin CH,Moustakas A
AM4300 was used in Western Blotting to demonstrate that transforming growth factor Β induces salt-inducible kinase 1 levels via a direct transcriptional mechanism, and characterise a putative enhancer element on the SIK1 gene.
Fri Apr 13 00:00:00 EDT 2012
Load-reducing therapy prevents development of arrhythmogenic right ventricular cardiomyopathy in plakoglobin-deficient mice.
Journal of the American College of Cardiology
Fabritz L,Hoogendijk MG,Scicluna BP,van Amersfoorth SC,Fortmueller L,Wolf S,Laakmann S,Kreienkamp N,Piccini I,Breithardt G,Noppinger PR,Witt H,Ebnet K,Wichter T,Levkau B,Franke WW,Pieperhoff S,de Bakker JM,Coronel R,Kirchhof P
AM4300 was used in western blot to test the effect of ventricular load on cardiomyopathy.
Tue Feb 08 00:00:00 EST 2011
Loss of special AT-rich binding protein 1 expression is a marker of poor survival in lung cancer.
Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer
Selinger CI,Cooper WA,Al-Sohaily S,Mladenova DN,Pangon L,Kennedy CW,McCaughan BC,Stirzaker C,Kohonen-Corish MR
AM4300 was used in western blot to evaluate the prognostic significance of SATB1 expression in lung cancer.
Fri Jul 01 00:00:00 EDT 2011
Patient mutation in AIRE disrupts P-TEFb binding and target gene transcription.
Nucleic acids research
Žumer K,Plemenitaš A,Saksela K,Peterlin BM
AM4300 was used in western blot to characterize a APECED patient mutation.
Sat Oct 01 00:00:00 EDT 2011
Cyclin D1 inhibits mitochondrial activity in B cells.
Cancer research
Tchakarska G,Roussel M,Troussard X,Sola B
AM4300 was used in Immunocytochemistry-immunoflourescence to show in an model of cyclin D1 fusion protein transduction in mature B cells, that, cyclin D1 inhibits mitochondrial activity.
Tue Mar 01 00:00:00 EST 2011
Molecular mechanisms for activation of the agouti-related protein and stimulation of appetite.
Diabetes
Ilnytska O,Stütz AM,Park-York M,York DA,Ribnicky DM,Zuberi A,Cefalu WT,Argyropoulos G
AM4300 was used in Western Blotting to determine molecular mechanisms for the activation of hypothalamic Agrp and identify compounds that stimulate appetite in mice.
Sat Jan 01 00:00:00 EST 2011
KiSS1 mediates platinum sensitivity and metastasis suppression in head and neck squamous cell carcinoma.
Oncogene
Jiffar T,Yilmaz T,Lee J,Hanna E,El-Naggar A,Yu D,Myers JN,Kupferman ME
AM4300 was used in Western Blotting to study the role of KiSS1 loss in biologically aggressive head and neck squamous cell carcinoma, and suggest potential targets for therapy in cisplatin-resistant cancers.
Thu Jul 14 00:00:00 EDT 2011
C-terminus of heat shock cognate 70 interacting protein increases following stroke and impairs survival against acute oxidative stress.
Antioxidants & redox signaling
Stankowski JN,Zeiger SL,Cohen EL,DeFranco DB,Cai J,McLaughlin B
AM4300 was used in western blot to test if C-terminus of HSC70 interacting protein upregulation enhances neural survival
Sun May 15 00:00:00 EDT 2011
C-terminus of heat shock cognate 70 interacting protein increases following stroke and impairs survival against acute oxidative stress.
Antioxidants & redox signaling
Stankowski JN,Zeiger SL,Cohen EL,DeFranco DB,Cai J,McLaughlin B
AM4300 was used in western blot to test if C-terminus of HSC70 interacting protein upregulation enhances neural survival
Sun May 15 00:00:00 EDT 2011
Development of an Fn14 agonistic antibody as an anti-tumor agent.
mAbs
Michaelson JS,Amatucci A,Kelly R,Su L,Garber E,Day ES,Berquist L,Cho S,Li Y,Parr M,Wille L,Schneider P,Wortham K,Burkly LC,Hsu YM,Joseph IB
AM4300 was used in western blot to assess modulation of the TWEAK-Fn4 pathway as a therapeutic for oncology.
Wed Jan 11 00:00:00 EST 2012
Cell cholesterol modulates metalloproteinase-dependent shedding of low-density lipoprotein receptor-related protein-1 (LRP-1) and clearance function.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
Selvais C,D'Auria L,Tyteca D,Perrot G,Lemoine P,Troeberg L,Dedieu S,Noël A,Nagase H,Henriet P,Courtoy PJ,Marbaix E,Emonard H
AM4300 was used in western blot to elucidate the mechanisms by which cholesterol regulates LRP-1 levels and function at the plasma membrane.
Mon Aug 01 00:00:00 EDT 2011
Protein Tyrosine Phosphatase 1B (PTP1B) deficiency accelerates hepatic regeneration in mice.
The American journal of pathology
Revuelta-Cervantes J,Mayoral R,Miranda S,González-Rodríguez A,Fernández M,Martín-Sanz P,Valverde AM
AM4300 was used in western blot to elucidate the role of PTP1B in liver regeneration.
Fri Apr 01 00:00:00 EDT 2011
Loss of VOPP1 overexpression in squamous carcinoma cells induces apoptosis through oxidative cellular injury.
Laboratory investigation; a journal of technical methods and pathology
Baras AS,Solomon A,Davidson R,Moskaluk CA
AM4300 was used in western blot to determine the role that VOPP1 has in human squamous cell carcinoma.
Mon Aug 01 00:00:00 EDT 2011
The molecular and cellular basis of variable craniofacial phenotypes and their genetic rescue in Twisted gastrulation mutant mice.
Developmental biology
Billington CJ,Ng B,Forsman C,Schmidt B,Bagchi A,Symer DE,Schotta G,Gopalakrishnan R,Sarver AL,Petryk A
AM4300 was used in western blot to examine the varied penetrance and expressivity of the Twisted gastrulation mutation in mice.
Fri Jul 01 00:00:00 EDT 2011
Endotoxin, zymosan, and cytokines decrease the expression of the transcription factor, carbohydrate response element binding protein, and its target genes.
Innate immunity
Feingold KR,Shigenaga JK,Patzek SM,Chui LG,Moser A,Grunfeld C
AM4300 was used in western blot to examine ChREBP expression during the acute phase response
Fri Apr 01 00:00:00 EDT 2011
Genetic analyses using a mouse cell cycle mutant identifies magoh as a novel gene involved in Cdk regulation.
Genes to cells : devoted to molecular & cellular mechanisms
Inaki M,Kato D,Utsugi T,Onoda F,Hanaoka F,Murakami Y
AM4300 was used in western blot to study the role in Cdk regulation of the novel gene magoh identified in a genetic screen of a murine cell cycle mutant
Tue Feb 01 00:00:00 EST 2011
Cell type-dependent gene regulation by Staufen2 in conjunction with Upf1.
BMC molecular biology
Miki T,Kamikawa Y,Kurono S,Kaneko Y,Katahira J,Yoneda Y
AM4300 was used in western blot to identify binding partners of Stau2 in dendritic cells
Wed Nov 16 00:00:00 EST 2011
PINCH1 is transcriptional regulator in podocytes that interacts with WT1 and represses podocalyxin expression.
PloS one
Wang D,Li Y,Wu C,Liu Y
AM4300 was used in western blot to determine if PINCH1 translocates to the nucleus and regulates gene expression.
Thu Feb 24 00:00:00 EST 2011
Vascular endothelial growth factor receptors VEGFR-2 and VEGFR-3 are localized primarily to the vasculature in human primary solid cancers.
Clinical cancer research : an official journal of the American Association for Cancer Research
Smith NR,Baker D,James NH,Ratcliffe K,Jenkins M,Ashton SE,Sproat G,Swann R,Gray N,Ryan A,Jürgensmeier JM,Womack C
AM4300 was used in western blot to examine VEGF receptor expression in tumor cells
Thu Jul 15 00:00:00 EDT 2010
Morphine- and CaMKII-dependent enhancement of GIRK channel signaling in hippocampal neurons.
The Journal of neuroscience : the official journal of the Society for Neuroscience
Nassirpour R,Bahima L,Lalive AL,Lüscher C,Luján R,Slesinger PA
AM4300 was used in western blot to investigate the effect of morphine on neuronal GIRK signaling
Wed Oct 06 00:00:00 EDT 2010
Reactive oxygen species control senescence-associated matrix metalloproteinase-1 through c-Jun-N-terminal kinase.
Journal of cellular physiology
Dasgupta J,Kar S,Liu R,Joseph J,Kalyanaraman B,Remington SJ,Chen C,Melendez JA
AM4300 was used in western blot to report the redox-responsive molecular signals that drive senescence-associated matrix metalloproteinase-1 expression
Fri Oct 01 00:00:00 EDT 2010
MicroRNA miR-137 regulates neuronal maturation by targeting ubiquitin ligase mind bomb-1.
Stem cells (Dayton, Ohio)
Smrt RD,Szulwach KE,Pfeiffer RL,Li X,Guo W,Pathania M,Teng ZQ,Luo Y,Peng J,Bordey A,Jin P,Zhao X
AM4300 was used in western blot to investigate the role of miR-137 in neuronal maturation
Tue Jun 01 00:00:00 EDT 2010
The Chlamydia muridarum-induced IFN-β response is TLR3-dependent in murine oviduct epithelial cells.
Journal of immunology (Baltimore, Md. : 1950)
Derbigny WA,Johnson RM,Toomey KS,Ofner S,Jayarapu K
AM4300 was used in western blot to study the role of TLR3 in the Chlamydia-induced IFN-beta response using oviduct epithelial cells
Wed Dec 01 00:00:00 EST 2010
Trpm4 differentially regulates Th1 and Th2 function by altering calcium signaling and NFAT localization.
Journal of immunology (Baltimore, Md. : 1950)
Weber KS,Hildner K,Murphy KM,Allen PM
AM4300 was used in western blot to examine the T cell subset responses to Ca(2+) signals
Wed Sep 01 00:00:00 EDT 2010
Disruption of calvarial ossification in E2f4 mutant embryos correlates with increased proliferation and progenitor cell populations.
Cell cycle (Georgetown, Tex.)
Miller ES,Berman SD,Yuan TL,Lees JA
AM4300 was used in western blot to determine the role of E2F4 in bone development using mutant mice
Thu Jul 01 00:00:00 EDT 2010
Murine norovirus-1 cell entry is mediated through a non-clathrin-, non-caveolae-, dynamin- and cholesterol-dependent pathway.
The Journal of general virology
Gerondopoulos A,Jackson T,Monaghan P,Doyle N,Roberts LO
AM4300 was used in Western Blotting to elucidate the mechanisms of murine norovirus-1 cell entry.
Tue Jun 01 00:00:00 EDT 2010
Murine norovirus-1 cell entry is mediated through a non-clathrin-, non-caveolae-, dynamin- and cholesterol-dependent pathway.
The Journal of general virology
Gerondopoulos A,Jackson T,Monaghan P,Doyle N,Roberts LO
AM4300 was used in Western Blotting to elucidate the mechanisms of murine norovirus-1 cell entry.
Tue Jun 01 00:00:00 EDT 2010
PepT1 mediates transport of the proinflammatory bacterial tripeptide L-Ala-{gamma}-D-Glu-meso-DAP in intestinal epithelial cells.
American journal of physiology. Gastrointestinal and liver physiology
Dalmasso G,Nguyen HT,Charrier-Hisamuddin L,Yan Y,Laroui H,Demoulin B,Sitaraman SV,Merlin D
AM4300 was used in western blot to test if l-Ala-gamma-d-Glu-meso-DAP is transported into intestinal epithelial cells via PepT1
Wed Sep 01 00:00:00 EDT 2010
Endothelial cells are susceptible to rapid siRNA transfection and gene silencing ex vivo.
Journal of vascular surgery
Andersen ND,Chopra A,Monahan TS,Malek JY,Jain M,Pradhan L,Ferran C,LoGerfo FW
AM4300 was used in immunocytochemistry and immunohistochemistry to use three rapid siRNA transfection techniques to silence endothelial genes in the human saphenous vein
Wed Dec 01 00:00:00 EST 2010
Progesterone inhibits apoptosis in part by PGRMC1-regulated gene expression.
Molecular and cellular endocrinology
Peluso JJ,Liu X,Gawkowska A,Lodde V,Wu CA
AM4300 was used in western blot to study the function of progesterone receptor membrane component-1 monomers and dimers
Fri May 14 00:00:00 EDT 2010
Comparison of two dimensional electrophoresis mouse colon proteomes before and after knocking out Aquaporin 8.
Journal of proteomics
Magdeldin S,Li H,Yoshida Y,Enany S,Zhang Y,Xu B,Fujinaka H,Yaoita E,Yamamoto T
AM4300 was used in western blot to study protein alterations that contribute to AQP8 regulation and trafficking
Fri Sep 10 00:00:00 EDT 2010
N-methyl-D-aspartate, hyperpolarization-activated cation current (Ih) and gamma-aminobutyric acid conductances govern the risk of epileptogenesis following febrile seizures in rat hippocampus.
The European journal of neuroscience
Ouardouz M,Lema P,Awad PN,Di Cristo G,Carmant L
AM4300 was used in Western Blotting to provide a new hypothesis of cellular changes following hyperthermic seizures in predisposed individuals, and may help in the design of therapeutic strategies to prevent epileptogenesis following prolonged febrile seizures.
Thu Apr 01 00:00:00 EDT 2010
Basic fibroblast growth factor elicits formation of interstitial axonal branches via enhanced severing of microtubules.
Molecular biology of the cell
Qiang L,Yu W,Liu M,Solowska JM,Baas PW
AM4300 was used in western blot to test if basic fibroblast growth factor enhances axonal branching
Fri Jan 15 00:00:00 EST 2010
Role of the cytoplasmic domain of the L1 cell adhesion molecule in brain development.
The Journal of comparative neurology
Nakamura Y,Lee S,Haddox CL,Weaver EJ,Lemmon VP
AM4300 was used in western blot to elucidate the function of the L1 cytoplasmic domain.
Thu Apr 01 00:00:00 EDT 2010
Cysteine 95 and other residues influence the regulatory effects of Histidine 69 mutations on Human Immunodeficiency Virus Type 1 protease autoprocessing.
Retrovirology
Huang L,Hall A,Chen C
AM4300 was used in Western Blotting to study the effects of different amino acid residues within the NL4-3 protease on modulating precursor structures for the regulation of protease autoprocessing in an HIV-infected cell.
Tue Mar 23 00:00:00 EDT 2010
Parkin is protective against proteotoxic stress in a transgenic zebrafish model.
PloS one
Fett ME,Pilsl A,Paquet D,van Bebber F,Haass C,Tatzelt J,Schmid B,Winklhofer KF
AM4300 was used in western blot to characterize parkin knockouts of Danio rerio
Fri Jul 30 00:00:00 EDT 2010
MicroRNA-7 modulates CD98 expression during intestinal epithelial cell differentiation.
The Journal of biological chemistry
Nguyen HT,Dalmasso G,Yan Y,Laroui H,Dahan S,Mayer L,Sitaraman SV,Merlin D
AM4300 was used in western blot to investigate if microRNAs regulate CD98 expression during intestinal epithelial cell differentiation and inflammation
Fri Jan 08 00:00:00 EST 2010
Role of sphingomyelin synthase in controlling the antimicrobial activity of neutrophils against Cryptococcus neoformans.
PloS one
Qureshi A,Subathra M,Grey A,Schey K,Del Poeta M,Luberto C
AM4300 was used in Western Blotting to identify the sphingolipid pathway as a requirement for the exertion of neutrophil killing activity against Cryptococcus neoformans.
Tue Dec 28 00:00:00 EST 2010
A novel screen using the Reck tumor suppressor gene promoter detects both conventional and metastasis-suppressing anticancer drugs.
Oncotarget
Murai R,Yoshida Y,Muraguchi T,Nishimoto E,Morioka Y,Kitayama H,Kondoh S,Kawazoe Y,Hiraoka M,Uesugi M,Noda M
AM4300 was used in Western Blotting to develop a novel screen using the Reck tumour suppressor gene promoter for detecting both conventional and metastasis-suppressing anticancer drugs.
Sun Aug 01 00:00:00 EDT 2010
Everolimus attenuates neointimal hyperplasia in cultured human saphenous vein grafts.
European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery
Semsroth S,Stigler RG,Bernecker OY,Ruttmann-Ulmer E,Troppmair J,Macfelda K,Bonatti JO,Laufer G
AM4300 was used in western blot to test if mammalian target of rapamycin inhibitor everolimus attenuates neointimal hyperplasia
Sun Mar 01 00:00:00 EST 2009
KIF5B gene sequence variation and response of cardiac stroke volume to regular exercise.
Physiological genomics
Argyropoulos G,Stütz AM,Ilnytska O,Rice T,Teran-Garcia M,Rao DC,Bouchard C,Rankinen T
AM4300 was used in western blot to perform a genome-wide linkage scan for endurance training-induced changes in stroke volume
Thu Jan 08 00:00:00 EST 2009
Age-dependent increases in interstitial collagenase and MAP Kinase levels are exacerbated by superoxide dismutase deficiencies.
Experimental gerontology
Dasgupta J,Kar S,Van Remmen H,Melendez JA
AM4300 was used in western blot to investigate the contribution of reactive oxygen species to the age-dependent increase in collagenase
Sat Aug 01 00:00:00 EDT 2009
Molecular cloning and characterization of the common marmoset huntingtin gene.
Gene
Hohjoh H,Akari H,Fujiwara Y,Tamura Y,Hirai H,Wada K
AM4300 was used in western blot to isolate and identify the huntingtin gene of the common marmoset (Callithrix jacchus)
Sun Mar 01 00:00:00 EST 2009
Intracellular signaling pathways control mitochondrial events associated with the development of ischemia/ reperfusion-associated damage.
Transplant international : official journal of the European Society for Organ Transplantation
Sucher R,Gehwolf P,Kaier T,Hermann M,Maglione M,Oberhuber R,Ratschiller T,Kuznetsov AV,Bösch F,Kozlov AV,Ashraf MI,Schneeberger S,Brandacher G,Ollinger R,Margreiter R,Troppmair J
AM4300 was used in Western Blotting to study the activity patterns of ERK, JNK, and p38 during ischaemia-reperfusion, and their role in the perturbation of mitochondrial reactive oxygen species and Ca2+ homeostasis.
Tue Sep 01 00:00:00 EDT 2009
Dynamic gene and protein expression patterns of the autism-associated met receptor tyrosine kinase in the developing mouse forebrain.
The Journal of comparative neurology
Judson MC,Bergman MY,Campbell DB,Eagleson KL,Levitt P
AM4300 was used in western blot to elucidate the neurodevelopmental influences of Met activation.
Fri Apr 10 00:00:00 EDT 2009
Binding of Silurus asotus lectin to Gb3 on Raji cells causes disappearance of membrane-bound form of HSP70.
Biochimica et biophysica acta
Sugawara S,Kawano T,Omoto T,Hosono M,Tatsuta T,Nitta K
AM4300 was used in western blot to study Silurus asotus lectin-induced heat shock protein 70 expression
Sun Feb 01 00:00:00 EST 2009
Sp1 trans-activates the murine H(+)-K(+)-ATPase alpha(2)-subunit gene.
American journal of physiology. Renal physiology
Yu Z,Li M,Zhang D,Xu W,Kone BC
AM4300 was used in western blot to test if the -144/-135 Sp element influences basal HKalpha2 gene transcription in murine inner medullary collecting duct cells
Wed Jul 01 00:00:00 EDT 2009
C. elegans ATAD-3 is essential for mitochondrial activity and development.
PloS one
Hoffmann M,Bellance N,Rossignol R,Koopman WJ,Willems PH,Mayatepek E,Bossinger O,Distelmaier F
AM4300 was used in western blot to elucidate the function of ATAD3
Fri Oct 30 00:00:00 EDT 2009
Tripartite motif protein 32 facilitates cell growth and migration via degradation of Abl-interactor 2.
Cancer research
Kano S,Miyajima N,Fukuda S,Hatakeyama S
AM4300 was used in western blot to determine the contribution of TRIM32 to carcinogenesis
Tue Jul 15 00:00:00 EDT 2008
Retinoic acid downregulates Rae1 leading to APC(Cdh1) activation and neuroblastoma SH-SY5Y differentiation.
Oncogene
Cuende J,Moreno S,Bolaños JP,Almeida A
AM4300 was used in western blot to explore whether retinoic acid promotes differentiation of human SH-SY5Y neuroblastoma cells by modulating Cdh1
Thu May 22 00:00:00 EDT 2008
Survival signaling by C-RAF: mitochondrial reactive oxygen species and Ca2+ are critical targets.
Molecular and cellular biology
Kuznetsov AV,Smigelskaite J,Doblander C,Janakiraman M,Hermann M,Wurm M,Scheidl SF,Sucher R,Deutschmann A,Troppmair J
AM4300 was used in western blot to elucidate how RAF controls cell survival
Tue Apr 01 00:00:00 EDT 2008
NHA-oc/NHA2: a mitochondrial cation-proton antiporter selectively expressed in osteoclasts.
Bone
Battaglino RA,Pham L,Morse LR,Vokes M,Sharma A,Odgren PR,Yang M,Sasaki H,Stashenko P
AM4300 was used in western blot to identify genes upregulated in RANKL-stimulated osteoclast precursor cells
Tue Jan 01 00:00:00 EST 2008
Arf GTPase-activating protein ASAP1 interacts with Rab11 effector FIP3 and regulates pericentrosomal localization of transferrin receptor-positive recycling endosome.
Molecular biology of the cell
Inoue H,Ha VL,Prekeris R,Randazzo PA
AM4300 was used in western blot to report that the BAR domain of ASAP1 is a protein binding site for FIP3
Wed Oct 01 00:00:00 EDT 2008
GATA3-driven Th2 responses inhibit TGF-beta1-induced FOXP3 expression and the formation of regulatory T cells.
PLoS biology
Mantel PY,Kuipers H,Boyman O,Rhyner C,Ouaked N,Rückert B,Karagiannidis C,Lambrecht BN,Hendriks RW,Crameri R,Akdis CA,Blaser K,Schmidt-Weber CB
AM4300 was used in Western Blot to describe a mechanism controlling inducible Treg polarisation, which is overruled by the Th2 differentiation pathway involving IL-4 and GATA3.
Sat Dec 01 00:00:00 EST 2007
Inhibition of apoptotic potency by ligand stimulated thyroid hormone receptors located in mitochondria.
Apoptosis : an international journal on programmed cell death
Saelim N,Holstein D,Chocron ES,Camacho P,Lechleiter JD
AM4300 was used in western blot to investigate the role of thyroid hormone receptors in apoptosis
Mon Oct 01 00:00:00 EDT 2007
BMP suppresses PTEN expression via RAS/ERK signaling.
Cancer biology & therapy
Beck SE,Carethers JM
AM4300 was used in western blot to analyze the effect of bone morphogenetic proteins on PTEN
Wed Aug 01 00:00:00 EDT 2007
LMO4 can interact with Smad proteins and modulate transforming growth factor-beta signaling in epithelial cells.
Oncogene
Lu Z,Lam KS,Wang N,Xu X,Cortes M,Andersen B
AM4300 was used in western blot to study the involvement of LMO4 in mesenchymal-epithelial signaling
Thu May 11 00:00:00 EDT 2006
Galectin-3 expression correlates with apoptosis of tumor-associated lymphocytes in human melanoma biopsies.
The American journal of pathology
Zubieta MR,Furman D,Barrio M,Bravo AI,Domenichini E,Mordoh J
AM4300 was used in Western Blotting to investigate 33 biopsies from 24 melanoma patients and describe the correlation between the expression of galectin-3 or galectin-1 and the level of apoptosis of tumor-associated lymphocytes using immunohistochemistry and an in situ nick translation assay.
Mon May 01 00:00:00 EDT 2006
