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Invitrogen™
RNase T1 (1 U/μL)
Optimized for researchers performing RNA structure, RNA sequencing, protein footprinting, and boundary experiments, Ambion™ RNase T1, RNA-Grade cleaves 3' ofRead more
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Catalog number AM2283
Price (USD)/ 200 µL
195.65
Online exclusive
198.00
Save 2.35 (1%)
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Price (USD)/ 200 µL
195.65
Online exclusive
198.00
Save 2.35 (1%)
Add to cart
RNase T1 (1 U/μL)
Catalog numberAM2283
Price (USD)/ 200 µL
195.65
Online exclusive
198.00
Save 2.35 (1%)
-
Add to cart
Optimized for researchers performing RNA structure, RNA sequencing, protein footprinting, and boundary experiments, Ambion™ RNase T1, RNA-Grade cleaves 3' of single-stranded G residues and is tested for purity to ensure that no contaminating ribonuclease activities are present that could cleave at unanticipated sites. Supplied in one tube of 200 μL (1U/ μL). RNase T1 can be used to perform boundary experiments to define the minimal RNA sequence required for selectable activities such as protein binding or catalysis. In addition to applications for RNA structural analysis, RNA-Grade ribonucleases can be used to map protein binding sites on RNAs by comparing cleavage patterns in the presence and absence of an RNA binding protein.
Unit Definitions: 100 units is the amount of enzyme that yields an increase in absorption at 260 nm of 0.01428 units per min at room temperature using 60 μg/mL yeast total RNA as a substrate.
Accessory Products: Other RNA-Grade ribonuclease available include RNase A (SKU# AM2274), which cleaves 3' of single-stranded C and U residues. Combinations of the single- and double-stranded specific ribonucleases can provide rapid analysis of the physical structure of an interesting RNA.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration1 U/μL
FormatTube
EnzymeRNase
Fidelity (vs. Taq)10X
For Use With (Application)Real Time PCR (qPCR)
No. of Reactions200
Product LineAmbion™
Product TypeRnase T1 PCR Kit
Quantity200 μL
Shipping ConditionDry Ice
SourceRNA
FormSolution
Unit Size200 µL
Contents & Storage
RNase T1, 10X RNA Structure Buffer, 1X Alkaline Hydrolysis Buffer, Yeast RNA, Precipitation/Inactivation Buffer, and Gel Loading Buffer II