Invitrogen™
RNAqueous™-4PCR Total RNA Isolation Kit
The RNAqueous™-4PCR Kit is used for phenol-free total RNA isolation from small tissue samples (0.5 mg–75 mg) or from cells Read more
Catalog number AM1914
Price (USD)/ 30 preps
304.00
-
Price (USD)/ 30 preps
304.00
RNAqueous™-4PCR Total RNA Isolation Kit
Catalog numberAM1914
Price (USD)/ 30 preps
304.00
-
The RNAqueous™-4PCR Kit is used for phenol-free total RNA isolation from small tissue samples (0.5 mg–75 mg) or from cells (∼100 cells to ∼1 x 107 cells), using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. DNA-free™ DNA removal reagents are included in the kit, making the isolated RNA especially suitable for RT-PCR. Features of this kit:
• DNA-free RNA for RT-PCR
• Rapid, phenol-free total RNA isolation
• Novel reagent for rapid and safe removal of DNase and divalent cations
Using the RNAqueous™-4PCR Kit
The RNAqueous™-4PCR Kit is designed for isolatation of RNA for applications such as RT-PCR, that would benefit from its robust genomic DNA removal capabilities. When used in conjunction with RNAlater™ Tissue Collection: RNA Stabilization Solution, most tissue samples can be stored at 4°C and then easily disrupted in standard microfuge tubes using the pestles provided in the kit. There is no need for snap-freezing in liquid nitrogen or grinding in a mortar and pestle.
• DNA-free RNA for RT-PCR
• Rapid, phenol-free total RNA isolation
• Novel reagent for rapid and safe removal of DNase and divalent cations
Using the RNAqueous™-4PCR Kit
The RNAqueous™-4PCR Kit is designed for isolatation of RNA for applications such as RT-PCR, that would benefit from its robust genomic DNA removal capabilities. When used in conjunction with RNAlater™ Tissue Collection: RNA Stabilization Solution, most tissue samples can be stored at 4°C and then easily disrupted in standard microfuge tubes using the pestles provided in the kit. There is no need for snap-freezing in liquid nitrogen or grinding in a mortar and pestle.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Isolation TechnologySilica Spin Column
Purification Time10 min. (After sample homogenization)
Elution Volume50–120 μL
Sample TypeTissue, Cells
Final Product TypeTotal RNA
High-throughput CompatibilityNot High-throughput Compatible (Manual)
For Use With (Application)RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Quantity30 Preps
Shipping ConditionBox 1: Dry Ice
Box 2: Room Temperature
Box 2: Room Temperature
Starting Material AmountTissue: ≤75 mg
Cells: ≤107
Cells: ≤107
Yield≤10 μg per 1 mg tissue
≤1 μg per 1 x 105 cells
≤1 μg per 1 x 105 cells
Unit Size30 preps
Contents & Storage
• 600 μL 10X DNase 1 Buffer; store at -20°C
• 45 μL DNase 1; -20°C
• 1 mL Formaldehye Load Dye; -20°C
• 100 μL Linear Acrylamide; -20°C
• 50 μL Positive Control Primers; -20°C
• 1 mL 5M Ammonium Acetate; -20°C
• 35 mL Water; 4°C
• 600 μL DNase Inactivation Reagent; 4°C
• 30 mL Lysis/Binding Solution; 4°C
• 25 mL Wash Solution #1; 4°C
• 35 mL Wash Solution 2/3 (concentrated); 4°C
• 3 Pestles; room temperature
• 30 Filter Cartridges; room temperature
• 60 Collection Tubes; room temperature
• 25 mL Elution Solution; room temperature
• 45 μL DNase 1; -20°C
• 1 mL Formaldehye Load Dye; -20°C
• 100 μL Linear Acrylamide; -20°C
• 50 μL Positive Control Primers; -20°C
• 1 mL 5M Ammonium Acetate; -20°C
• 35 mL Water; 4°C
• 600 μL DNase Inactivation Reagent; 4°C
• 30 mL Lysis/Binding Solution; 4°C
• 25 mL Wash Solution #1; 4°C
• 35 mL Wash Solution 2/3 (concentrated); 4°C
• 3 Pestles; room temperature
• 30 Filter Cartridges; room temperature
• 60 Collection Tubes; room temperature
• 25 mL Elution Solution; room temperature