Invitrogen™
MEGAclear™ Transcription Clean-Up Kit
The MEGAclear™ Kit is designed for the quick cleanup of any large-scale transcription reaction. The MEGAclear™ Kit efficiently separates RNA Read more
Catalog number AM1908
Price (USD)/ 20 preps
178.00
-
Price (USD)/ 20 preps
178.00
MEGAclear™ Transcription Clean-Up Kit
Catalog numberAM1908
Price (USD)/ 20 preps
178.00
-
The MEGAclear™ Kit is designed for the quick cleanup of any large-scale transcription reaction. The MEGAclear™ Kit efficiently separates RNA from unincorporated NTPs, enzymes, and buffer components. Advantages of the MEGAclear™ Kit:
• Purifies transcription reactions to produce high-quality RNA, free of unincorporated NTPs, enzymes, and buffer components
• High recovery from 1 ng to 500 μg of input
• Ideal for purification of aRNA amplification reactions, nonisotopically labeled RNA probes, and capped RNA transcripts for oocyte injections
• Quick protocol—purify RNA in less than 30 minutes
Using the MEGAclear™ Kit
The protocol is quick and easy. The transcription reaction is mixed with Binding Solution, passed over an RNA-binding filter, and washed; an elution step releases the purified RNA, which is now ready for any application requiring highly pure synthetic or in vitrotranscribed RNA. For RNA recovery of 70% or greater, single-stranded RNA should be >100 nt and double-stranded RNA should be >200 bp.
• Purifies transcription reactions to produce high-quality RNA, free of unincorporated NTPs, enzymes, and buffer components
• High recovery from 1 ng to 500 μg of input
• Ideal for purification of aRNA amplification reactions, nonisotopically labeled RNA probes, and capped RNA transcripts for oocyte injections
• Quick protocol—purify RNA in less than 30 minutes
Using the MEGAclear™ Kit
The protocol is quick and easy. The transcription reaction is mixed with Binding Solution, passed over an RNA-binding filter, and washed; an elution step releases the purified RNA, which is now ready for any application requiring highly pure synthetic or in vitrotranscribed RNA. For RNA recovery of 70% or greater, single-stranded RNA should be >100 nt and double-stranded RNA should be >200 bp.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Final Product TypePurified Synthetic mRNA, Purified mRNA
FormatTube
Isolation TechnologySilica Spin Column
Purification TargetmRNA, Synthetic mRNA
Sample TypemRNA, Synthetic mRNA
For Use With (Application)In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
For Use With (Equipment)Vacuum Manifold, Centrifuge
High-throughput CompatibilityNot High-throughput Compatible (Manual)
No. of Reactions20 Preps
Product LineAmbion™, MEGAclear™
Product TypemRNA purification kit, Purification kit
Quantity20 Preps
Purification Time30 min
Shipping ConditionRoom Temperature
Starting Material Volume1 ng to 500 μg of dsRNA>200b or ssRA>100bp
Yield500 μg
Unit Size20 preps
Contents & Storage
Binding Solution, Wash Solution Concentrate, and 5 M Ammonium Acetate are stored at 4°C. Elution Solution can be stored at any temperature, and Filter Cartridges and Collection and Elution Tubes are stored at room temperature.