Invitrogen™
MEGAscript™ T7 Transcription Kit
The MEGAscript™ T7 Kit is an ultra-high yield in vitro transcription kit. The high yields are achieved by modifying typical Read more
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| Catalog Number | Product Type | No. of Reactions |
|---|---|---|
| AM1334 | T7 In Vitro Transcription kit | 40 Reactions |
| AMB13345 | T7 In Vitro Transcription kit | 200 Reactions |
| AM1333 | T7 In Vitro Transcription kit | 25 Reactions |
Catalog number AM1334
Price (USD)/ 40 reactions
407.65
Online exclusive
440.00 Save 32.35 (7%)
-
Product Type:
T7 In Vitro Transcription kit
No. of Reactions:
40 Reactions
Price (USD)/ 40 reactions
407.65
Online exclusive
440.00 Save 32.35 (7%)
MEGAscript™ T7 Transcription Kit
Catalog numberAM1334
Price (USD)/ 40 reactions
407.65
Online exclusive
440.00 Save 32.35 (7%)
-
The MEGAscript™ T7 Kit is an ultra-high yield in vitro transcription kit. The high yields are achieved by modifying typical transcription reaction conditions so that very high nucleotide concentrations can be effectively used. The T7 Enzyme Mix and the 10X Reaction Buffer are specifically calibrated for each lot and RNA polymerase.
Using the MEGAscript™ T7 Kit
The MEGAscript™ T7 Kit contains in vitro transcription reaction components and a control template. The kit will yield a total of 3–5 mg of RNA (approximately 100 μg of RNA or more per reaction) from the control template supplied with the kit. This corresponds to 400–650 moles of RNA for each mole of template. Smaller templates typically yield a lower mass and a higher molar yield of product. Novel transcription reaction conditions and a patented, high-yield technology allow the synthesis of 10–50 times the amount of RNA produced by conventional transcription reactions.
Applications
The MEGAscript™ T7 Kit is intended for the synthesis of large amounts of unlabeled or low specific activity RNA for a variety of uses, including in vitro translation, antisense/microinjection studies, and isolation of RNA binding proteins. In large-scale transcription reactions, the concentration of all 4 nucleotides is high, well above the Km for the enzyme. The MEGAscript™ T7 Kit typically yields over 10 times more RNA than conventional in vitro transcription reactions. The kit is not recommended for synthesis of high specific activity probes.
Accessory products
MessageAmp™ aRNA amplification kits are powered by MEGAscript™ technology and have been developed especially for the amplification of cRNA prior to array analysis. To purify in vitro transcription products from free nucleotides, buffer components, and enzymes, we recommend using the MEGAclear™ Kit (Cat. No. AM1908) and the MEGAclear™-96 Kit (Cat. No. AM1909).
Using the MEGAscript™ T7 Kit
The MEGAscript™ T7 Kit contains in vitro transcription reaction components and a control template. The kit will yield a total of 3–5 mg of RNA (approximately 100 μg of RNA or more per reaction) from the control template supplied with the kit. This corresponds to 400–650 moles of RNA for each mole of template. Smaller templates typically yield a lower mass and a higher molar yield of product. Novel transcription reaction conditions and a patented, high-yield technology allow the synthesis of 10–50 times the amount of RNA produced by conventional transcription reactions.
Applications
The MEGAscript™ T7 Kit is intended for the synthesis of large amounts of unlabeled or low specific activity RNA for a variety of uses, including in vitro translation, antisense/microinjection studies, and isolation of RNA binding proteins. In large-scale transcription reactions, the concentration of all 4 nucleotides is high, well above the Km for the enzyme. The MEGAscript™ T7 Kit typically yields over 10 times more RNA than conventional in vitro transcription reactions. The kit is not recommended for synthesis of high specific activity probes.
Accessory products
MessageAmp™ aRNA amplification kits are powered by MEGAscript™ technology and have been developed especially for the amplification of cRNA prior to array analysis. To purify in vitro transcription products from free nucleotides, buffer components, and enzymes, we recommend using the MEGAclear™ Kit (Cat. No. AM1908) and the MEGAclear™-96 Kit (Cat. No. AM1909).
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Final Product TypeSynthetic mRNA, mRNA
FormatKit
Sample TypemRNA, Synthetic mRNA
For Use With (Application)In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
No. of Reactions40 Reactions
Product LineAmbion™, MEGAscript™
Product TypeT7 In Vitro Transcription kit
Quantity40 Reactions
PromoterT7
Shipping ConditionDry Ice
Yield100 μg
Starting MaterialPCR Products, Linearized DNA plasmid
Unit Size40 reactions
Contents & Storage
T7 Enzyme Mix, 10X Reaction Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, and Gel Loading Buffer II are all stored at –20°C. Nuclease-free Water may be stored at any temperature.