CTS™ Detachable Dynabeads™ CD3/CD28

Gibco CTS Detachable Dynabeads CD3/CD28 magnetic beads are intended for ex vivo isolation and activation of T cells in a simultaneous one-step mechanism. When used in combination with the CTS Detachable Dynabeads Release Buffer (Cat. No. A55883), this offering is the first-of-its-kind cGMP cell selection technology to have an active release mechanism for process development, clinical trial, and commercial manufacturing use.

Features of the CTS Detachable Dynabeads CD3/CD28 include:

• Empower users with process flexibility by allowing active release of beads from target cells at any time
• Optimize target cell purity, yield, activation profile, and desired phenotype thanks to the active release mechanism that gives users more control
• Provide an automation-friendly solution by working directly with the CTS DynaCellect Magnetic Separation System
• Enable consistent performance in light of biological variability within processes
• Are suitable for both autologous and allogeneic cell therapy manufacturing processes

Coated with camelid-derived single-domain VHH antibodies that target CD3 and CD28 cell surface markers, these Dynabeads magnetic beads provide both the primary and co-stimulatory signals that are required for activation and expansion of T cells. These Detachable Dynabeads are meant for direct use with the CTS Detachable Dynabeads Release Buffer that has an active ingredient, biotin, that competitively displaces a biotin derivative on the surface of the beads. This proprietary mechanism allows users to achieve active release of the beads from target cells at any time within their process.

Based on a recommended 3-to-1 bead-to-target-cell ratio (not total nucleated cell number), one 10-mL vial can theoretically isolate and activate up to 1.33 x 10⁹ T cells.

CTS Detachable Dynabeads CD3/CD28 also include Drug Master Files (DMFs) to support cross-referencing in regulatory submissions prior to clinical trial and commercial manufacturing use.