The Pierce LC-MS/MS System Suitability Standard (7 x 5 mixture) contains seven PRTC HeavyPeptide AQUA Ultimate peptides in five isotopologue versions for the generation of seven standard curves to assess sensitivity and dynamic range of LC-MS/MS systems.
The Pierce LC-MS/MS System Suitability Standard (7 x 5 mix) contains 7 of 15 peptides from the Pierce Peptide Retention Time Calibration Mixture (Cat. No. 88320). Each of the seven peptides has five versions (amino acids labeled with 0, 1, 2, 3, or 4 heavy isotopes) at five distinct concentrations. The 7 x 5 mix can be used with Skyline software to assess dynamic range, linearity, and lower limit of quantitation (LLOQ).
The Pierce LC-MS/MS System Suitability Standard (7 x 5 mixture) is:
• High quality—contains five sets of seven HeavyPeptide AQUA Ultimate peptides, each distinguishable through unique isotopologue labeling
• Optimized—enables scheduling of MS acquisition windows for improved quantification and increased multiplexing
• Quantitative—assesses dynamic range of instrument and instrument sensitivity
• Predictive—enables assessment of chromatography and MS instrument performance
Applications:
• Optimization of LC & MS parameters
• Standard for data-dependent acquisition (DDA)
• Standard for targeted MS (e.g., parallel-reaction monitoring (PRM)
• Identification of total peptide elution window
• Assessment dynamic range of nano or capillary flow LC-MS/MS systems