Catalog Number | Quantity |
---|---|
A47996 | 10 μg |
A47997 | 5 x 10 μg |
The Thermo Scientific Pierce HeLa Digest/PRTC Standard is a validated complex mammalian protein digest containing additional PRTC heavy isotope-labeled peptides designed for LC/MS performance testing.
Features of HeLa Digest/PRTC Standard include:
The Pierce HeLa Digest/PRTC Standard is a lyophilized mixture of 10 μg of HeLa protein digest with 5 pmol PRTC peptides to assess chromatography MS instrument performance. The protein digest is derived from a well-established adenocarcinoma reference HeLa S3 cell line that expresses over 15,000 proteins with relevant post-translational modifications. This complex digest is an ideal standard for complex proteome mass spectrometry applications.
The included Pierce Peptide Retention Time Calibration Mixture contains 15 synthetic heavy isotope-labeled peptides mixed at an equimolar ratio that elute across the chromatographic gradient. The observed retention times and hydrophobicity factors (HFs) for these calibrant peptides are fit to a linear equation to determine the slope of the retention time/HF relationship. This equation and the HF of an uncharacterized peptide are then used to predict retention time. In combination, the mixture is a powerful tool to optimize and assess LC parameters, identify the total peptide elution window, and optimize and monitor MS instrumentation.
This standard is specifically formulated for LC/MS experiments and does not contain salts or detergents. Using the standard routinely before analysis of complex samples makes it possible to monitor and normalize LC/MS performance between samples and over time.
Applications: