TaqMan™ SCID/SMA Assay

The Applied Biosystems TaqMan SCID/SMA Assay is a multiplex real-time PCR assay that detects the SMN1 gene and TREC, as well as the RNase P gene as an internal genomic control. For an assay that also detects KREC, please see the TaqMAN SCID/SMA Plus Assay (Cat. No. A48566).

Features of the TaqMan SCID/SMA Assay include:
• Optimized for SCID and SMA
• Direct extraction from DBS samples for reduced hands-on time and cost
• Compatible with 96- or 384-reaction protocols
• High-quality results for 96 samples within 55 min using the fast cycling protocol
• Minimal liquid-dispensing steps for easy automation

Complete solution from DBS to result
The TaqMan SCID/SMA Assay detects SMN1, TREC, as well as the RNase P gene as an internal genomic control. The assay solution contains a mixture of primers and probes for each target plus an SMN2 blocker. From DBS to result, less than 40 min of hands-on time and only four reagent-dispensing steps are required for 96-well plates. One additional pipetting transfer step from DBS lysate extraction plate to qPCR plate is needed. A real-time PCR instrument with at least five filters is required to detect the reporter dyes plus the passive reference dye.

The TaqMan SCID/SMA Assay is a multiplex real-time PCR assay that detects exon 7 of SMN1 and TREC. The assay includes the RNase P gene as an internal amplification control, verifying successful sample extraction. The assay is optimized for high specificity to SMN1, eliminating cross-detection of SMN2.