Applied Biosystems™
VetMAX™ MastiType Multi Kit
The VetMAX MastiType Multi Kit enables rapid, accurate detection of 15 mastitis-causing pathogens and one penicillin-resistance gene in bovine milk. Read more
Catalog number A39227
Price (USD)/ 100 tests
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The VetMAX MastiType Multi Kit enables rapid, accurate detection of 15 mastitis-causing pathogens and one penicillin-resistance gene in bovine milk. These 16 markers are detected using real-time PCR amplification of DNA unique to each pathogen in four separate reactions.
The advanced technology of the VetMAX MastiType real-time PCR assays offers these advantages:
• Designed for laboratories requiring high-throughput processing methods
• Faster results—typical time from sample to result is 2.5–3 hours, compared to up to 10 days for a culture
• High accuracy—high sensitivity and specificity for all individual pathogens
• Identifies and quantifies DNA, allowing detection of viable, dead, and growth-inhibited pathogens
• Allows use of preserved milk samples, eliminating the need for cooling during sample transportation
• Can be integrated into milk recording programs using preserved milk
• Validated with convenient MagMAX CORE nucleic acid purification method that does not require aspiration and centrifugation
The VetMAX MastiType Multi assay detects these 15 pathogens and one resistance gene in four separate PCR reactions:
• Staphylococcus aureus
• Staphylococcus spp. (including all major coagulase-negative staphylococci)
• Streptococcus agalactiae
• Streptococcus dysgalactiae
• Streptococcus uberis
• Escherichia coli
• Enterococcus spp. (including E. faecalis and E. faecium)
• Klebsiella oxytoca (and/or K. pneumoniae)
• Serratia marcescens
• Corynebacterium bovis
• Trueperella pyogenes and/or Peptoniphilus indolicus
• Staphylococcal β-lactamase gene (penicillin-resistance gene)
• Mycoplasma bovis
• Mycoplasma spp.
• Yeast
• Prototheca spp.
The advanced technology of the VetMAX MastiType real-time PCR assays offers these advantages:
• Designed for laboratories requiring high-throughput processing methods
• Faster results—typical time from sample to result is 2.5–3 hours, compared to up to 10 days for a culture
• High accuracy—high sensitivity and specificity for all individual pathogens
• Identifies and quantifies DNA, allowing detection of viable, dead, and growth-inhibited pathogens
• Allows use of preserved milk samples, eliminating the need for cooling during sample transportation
• Can be integrated into milk recording programs using preserved milk
• Validated with convenient MagMAX CORE nucleic acid purification method that does not require aspiration and centrifugation
The VetMAX MastiType Multi assay detects these 15 pathogens and one resistance gene in four separate PCR reactions:
• Staphylococcus aureus
• Staphylococcus spp. (including all major coagulase-negative staphylococci)
• Streptococcus agalactiae
• Streptococcus dysgalactiae
• Streptococcus uberis
• Escherichia coli
• Enterococcus spp. (including E. faecalis and E. faecium)
• Klebsiella oxytoca (and/or K. pneumoniae)
• Serratia marcescens
• Corynebacterium bovis
• Trueperella pyogenes and/or Peptoniphilus indolicus
• Staphylococcal β-lactamase gene (penicillin-resistance gene)
• Mycoplasma bovis
• Mycoplasma spp.
• Yeast
• Prototheca spp.
Specifications
Detection MethodPrimer-probe
GC-Rich PCR PerformanceHigh
PCR MethodMultiplex qPCR
Reaction SpeedFast or Standard
For Use With (Equipment)7500 Fast System, 7500 System, QuantStudio™
Product LineVetMAX™
Product TypePCR Reagents and Detection Kit for Animal Health
Quantity100 tests
Shipping ConditionDry Ice
No. of Reactions4 Reactions
Sample TypeDNA
Unit Size100 tests
Contents & Storage
• 4 x 1.2 mL MastiType Master Mix: contains a Hot Start DNA polymerase in an optimized PCR buffer with magnesium and dNTPs
• 1 x 440 μL MastiType Positive Control: a positive control for the PCR reaction components
• 1 x 550 μL MastiType Multi Primer Mix 1: includes primers for multiplex PCR amplification of Staph. aureus, M. bovis, Enterococcus spp. (incl. E. faecalis and E. faecium), Corynebacterium bovis, and an Internal Amplification Control (IAC)
• 1 x 550 μL MastiType Multi Primer Mix 2: includes primers for multiplex PCR amplification of Staphylococcal ß-lactamase gene (penicillin-resistance gene), E. coli, Strep. dysgalactiae, Mycoplasma spp, and an Internal Amplification Control (IAC)
• 1 x 550 μL MastiType Multi Primer Mix 3: includes primers for multiplex PCR amplification of Staphylococcus spp. (including all major CNS), Streptococcus agalactiae, Streptococcus uberis, Prototheca spp
• 1 x 550 μL MastiType Multi Primer Mix 4: includes primers for multiplex PCR amplification of yeast, Serratia marcescens, Klebsiella oxytoca and/or K. pneumonia, Trueperella pyogenes and/or Peptoniphilus indolicus
Store at -20°C.
• 1 x 440 μL MastiType Positive Control: a positive control for the PCR reaction components
• 1 x 550 μL MastiType Multi Primer Mix 1: includes primers for multiplex PCR amplification of Staph. aureus, M. bovis, Enterococcus spp. (incl. E. faecalis and E. faecium), Corynebacterium bovis, and an Internal Amplification Control (IAC)
• 1 x 550 μL MastiType Multi Primer Mix 2: includes primers for multiplex PCR amplification of Staphylococcal ß-lactamase gene (penicillin-resistance gene), E. coli, Strep. dysgalactiae, Mycoplasma spp, and an Internal Amplification Control (IAC)
• 1 x 550 μL MastiType Multi Primer Mix 3: includes primers for multiplex PCR amplification of Staphylococcus spp. (including all major CNS), Streptococcus agalactiae, Streptococcus uberis, Prototheca spp
• 1 x 550 μL MastiType Multi Primer Mix 4: includes primers for multiplex PCR amplification of yeast, Serratia marcescens, Klebsiella oxytoca and/or K. pneumonia, Trueperella pyogenes and/or Peptoniphilus indolicus
Store at -20°C.