DMEM for SILAC
DMEM for SILAC
Thermo Scientific™

DMEM for SILAC

Analyze protein expression by mass spectrometry using these media or additives for stable isotope labeling with amino acids in cell culture (SILAC).
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Catalog NumberQuantity
A338226 x 500 mL
88364500 mL
Catalog number A33822
Price (USD)/ 6 x 500 mL
284.65
Online exclusive
300.00 
Save 15.35 (5%)
-
Add to cart
Quantity:
6 x 500 mL
Price (USD)/ 6 x 500 mL
284.65
Online exclusive
300.00 
Save 15.35 (5%)
Add to cart
DMEM for SILAC
Catalog numberA33822
Price (USD)/ 6 x 500 mL
284.65
Online exclusive
300.00 
Save 15.35 (5%)
-
Add to cart
Thermo Scientific™ DMEM for SILAC is optimized for use with stable isotope labeling with amino acids in cell culture (SILAC) to analyze protein expression by mass spectrometry (MS).

Features of DMEM for SILAC:

  • Flexible—liquid media deficient in both L-lysine and L-arginine, allowing for more complete proteome coverage through multiple isotopic amino acid labeling
  • High quality—medium is sterile, endotoxin-free, and cell culture-compatible

SILAC is a powerful method to identify and quantify relative differential changes in complex protein samples. This approach entails the in vivo metabolic incorporation of 'heavy' 13C- or 15N-labeled amino acids into proteins followed by mass spectrometry (MS) for the accelerated and comprehensive identification, characterization, and quantitation of proteins.

This SILAC medium is used together with heavy and light amino acids for the differential isotopic labeling of cells for SILAC analysis. SILAC media supplemented with heavy amino acids are capable of nearly 100% label incorporation into proteins of living cells.

Related products

  • NeuCode™ Lysine -080, 50 mg (A33613)
  • Fetal Bovine Serum, dialyzed, US origin (26400036)
  • RPMI 1640 Media for SILAC (88365)
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Equipment)Mass Spectrometer
Product TypeMedia
Label or DyeMass Isotope of Amino Acid
Shipping ConditionRoom Temperature
Starting MaterialCell Lysate
Final Product TypeLiquid Medium
Quantity6 x 500 mL
FormLiquid
Unit Size6 x 500 mL
Contents & Storage
Store at 2°C to 8°C.