Invitrogen

Human MLK4, GST Tag Recombinant Protein

Product Details

A33382

Applications

Tested Dilution

Publications

Kinase Assay (KA)

Assay-dependent

Product Specifications

Species

Human

Expression System

Insect cells

Amino acid sequence

114-420

Tag

GST-tag

Molecular weight

60.5 kDa

Class

Recombinant

Type

Protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

purified

Storage buffer

50mM tris HCl, pH 7.5, with 10mM glutathione, 0.1mM PMSF, 150mM NaCl, 25% glycerol, 0.25mM DTT, 0.1mM EDTA

Contains

no preservative

Storage conditions

-80° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Dry ice

Product Specific Information

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 µg, 10 µg and 20 µg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 µL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase.

This MLK4 recombinant human protein (114-420) was expressed in insect cells.

MLK4 recombinant human protein is also available in our SelectScreen™ Kinase Profiling Service.

• Validated for use with the LanthaScreen™ Eu Kinase Binding assay
• Available in multiple pack sizes for convenient use
• Also available in our SelectScreen Kinase Profiling Service

Protein Form: Recombinant, Active, Catalytic

Target Information

MLK4 or mixed lineage kinase 4 belongs to the superfamily of MAP kinase kinases (MAP3K1) which contain both ser/thr and tyr kinases activity in their catalytic domains. The structure of this kinase family incorporates an N-terminal Src homology (SH3) domain, followed by the kinase domain, a leucine zipper region, and a CDC42 /RAC -interactive binding (CRIB) motif, but divergent C-terminal regions. MLK4 is highly expressed in kidney and pancreas. MLK4 is a negative regulator of TLR4 and does not activate JNK1/MAPK8 pathway, p38/MAPK14, nor ERK2/MAPK1 pathways.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Mitogen-activated protein kinase kinase kinase 21; Mitogen-activated protein kinase kinase kinase MLK4; Mixed lineage kinase 4; MLK4