Invitrogen™
TRIzol™ Plus RNA Purification Kit and Phasemaker™ Tubes Complete System
This system includes both the TRIzol™ Plus RNA Purification Kit and Invitrogen™ Phasemaker™ Tubes. The TRIzol Plus RNA Purification Kit Read more
Catalog number A33254
Price (USD)/ 50 preparations
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This system includes both the TRIzol™ Plus RNA Purification Kit and Invitrogen™ Phasemaker™ Tubes.
The TRIzol Plus RNA Purification Kit combines the strong lysis capability of TRIzol Reagent with the convenient and time-saving silica-cartridge purification protocol of the PureLink™ RNA Mini Kit to provide ultrapure total RNA typically within an hour from a wide variety of sample types.
Phasemaker Tubes contain a thick liquid polymer that separates and completely isolates the upper aqueous phase of the TRIzol Reagent mix from the organic phase underneath, greatly simplifying the removal of the upper phase containing the RNA. This TRIzol Plus RNA Purification Kit and Phasemaker Tubes Complete System is for the extraction of RNA, not DNA or protein. It will accommodate 50–100 mg of tissue. For cultured cells, please refer to the manual for information on sample size.
The TRIzol Plus RNA Purification Kit combines the strong lysis capability of TRIzol Reagent with the convenient and time-saving silica-cartridge purification protocol of the PureLink™ RNA Mini Kit to provide ultrapure total RNA typically within an hour from a wide variety of sample types.
Phasemaker Tubes contain a thick liquid polymer that separates and completely isolates the upper aqueous phase of the TRIzol Reagent mix from the organic phase underneath, greatly simplifying the removal of the upper phase containing the RNA. This TRIzol Plus RNA Purification Kit and Phasemaker Tubes Complete System is for the extraction of RNA, not DNA or protein. It will accommodate 50–100 mg of tissue. For cultured cells, please refer to the manual for information on sample size.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Isolation TechnologySilica Spin Column, Organic Extraction
Purification Time1 hr.
Elution Volume30 to 200 μL
Sample TypeBacteria, Cells, Plants, Tissue, Yeast
Final Product TypeTotal RNA
High-throughput CompatibilityNot High-throughput Compatible (Manual)
For Use With (Application)RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling
Quantity50 Preps
ScaleMini
Shipping ConditionRoom Temperature
Starting Material AmountBacteria: ≤107
Cells: ≤107
Plant: ≤107, ≤100 mg
Tissue: ≤100 mg
Yeast: ≤107
Cells: ≤107
Plant: ≤107, ≤100 mg
Tissue: ≤100 mg
Yeast: ≤107
Yield≤350 μg
Unit Size50 preparations
Contents & Storage
• 100 mL TRIzol Reagent; room temperature
• 100 Phasemaker Tubes; room temperature
• 1 PureLink RNA Mini Kit
• 125 mL Lysis Buffer; room temperature
• 50 mL Wash Buffer I; room temperature
• 15 mL Wash Buffer II; room temperature
• 15.5 mL RNase-free Water; room temperature
• 50 PureLink Spin Cartridges with Collection Tubes; room temperature
• 50 Collection Tubes; room temperature
• 50 Recovery Tubes; room temperature