Invitrogen

Human TEK (TIE2) [Y897S], GST Tag Recombinant Protein

Datasheet

Tech Support

Datasheet

Tech Support

Product Details

A32878

Applications

Tested Dilution

Publications

Kinase Assay (KA)

Assay-dependent

Product Specifications

Species

Human

Expression System

Insect cells

Amino acid sequence

771-end

Tag

GST-tag

Molecular weight

66.7 kDa

Class

Recombinant

Type

Protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

purified

Storage buffer

50mM tris HCl, pH 7.5, with 0.1mM PMSF, 0.25mM DTT, 25% glycerol, 150mM NaCl, 0.1mM EDTA, 10mM glutathione

Contains

no preservative

Storage conditions

-80° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Dry ice

Product Specific Information

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 µg, 10 µg and 20 µg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 µL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase.

This TEK (TIE2) Y897S recombinant human protein (771-end) was expressed in insect cells.

TEK (TIE2) Y897S recombinant human protein is also available in our SelectScreen™ Kinase Profiling Service.

• Validated for use with the Z'-LYTE™ assay
• Available in multiple pack sizes for convenient use
• Also available in our SelectScreen Kinase Profiling Service

Protein Form: Recombinant, Active, Catalytic

Target Information

TEK (TIE2) Y897S receptor tyrosine kinase is expressed principally on vascular endothelium. Disrupting TEK Y897S function in mice results in embryonic lethality with defects in embryonic vasculature, suggesting a role in blood vessel maturation and maintenance. Angiopoietin-1 is a secreted growth factor that binds to and activates the TEK receptor tyrosine kinase. SHP2 and GRB2 are recruited to the activated TEK kinase domain and are part of the cellular responses that mediate TEK function. TEK expression is upregulated in the endothelium of vascular 'hot spots' in human breast cancer specimens. However, TEK is also overexpressed in areas of active angiogenesis in normal tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Angiopoietin-1 receptor; CD202b; Endothelial tyrosine kinase; hTIE2; p140 TEK; TEK tyrosine kinase, endothelial; TIE2; Tunica interna endothelial cell kinase; Tyrosine kinase with Ig and EGF homology domains-2; Tyrosine-protein kinase receptor TEK; Tyrosine-protein kinase receptor TIE-2