Invitrogen

Human NIM1, GST Tag Recombinant Protein

Product Details

A32873

Applications

Tested Dilution

Publications

Kinase Assay (KA)

Assay-dependent

Product Specifications

Species

Human

Expression System

Insect cells

Amino acid sequence

Full length

Tag

GST-tag

Molecular weight

75.9 kDa

Class

Recombinant

Type

Protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

purified

Storage buffer

50mM tris HCl, pH 7.5, with 0.1mM PMSF, 0.25mM DTT, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 25% glycerol

Contains

no preservative

Storage conditions

-80° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Dry ice

Product Specific Information

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 µg, 10 µg and 20 µg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 µL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase.

This NIM1K recombinant human protein (full length) was expressed in insect cells.

NIM1K recombinant human protein is also available in our SelectScreen™ Kinase Profiling Service.

• Validated for use with the Z'-LYTE™ assay
• Available in multiple pack sizes for convenient use
• Also available in our SelectScreen Kinase Profiling Service

Protein Form: Full Length, Recombinant, Active

Target Information

NIM1K serine/threonine kinase or NIM1 belongs to CAMK kinase family. Unlike to other AMPK-related kinases, NIM1 cannot be phosphorylated and activated by LKB1. Two somatic mutations (P333S and P411T) were found in lung neuroendocrine carcinoma and lung large cell carcinoma samples. NIM1 gene ontology (GO) annotations include cellular response to glucose starvation; intracellular signal transduction; negative regulation of TOR signaling; protein phosphorylation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: NIM1 serine/threonine-protein kinase; Serine/threonine-protein kinase NIM1