Invitrogen

Human PI4K2B (PI4K2 beta), GST Tag Recombinant Protein

Product Details

A31080

Applications

Tested Dilution

Publications

Kinase Assay (KA)

Assay-dependent

Product Specifications

Species

Human

Expression System

Insect cells

Amino acid sequence

Full length

Tag

GST-tag

Molecular weight

82.1 kDa

Class

Recombinant

Type

Protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

purified

Storage buffer

50mM tris HCl, pH 7.5, with 25% glycerol, 0.1mM EDTA, 0.1mM PMSF, 150mM NaCl, 10mM glutathione, 0.25mM DTT

Contains

no preservative

Storage conditions

-80° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Dry ice

Product Specific Information

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 µg, 10 µg and 20 µg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 µL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase.

This PI4K2B (PI4K2 beta) recombinant human protein (full length) was expressed in insect cells.

PI4K2B (PI4K2 beta) recombinant human protein is also available in our SelectScreen™ Kinase Profiling Service.

• Validated for use with the Adapta™ assay
• Available in multiple pack sizes for convenient use
• Also available in our SelectScreen Kinase Profiling Service

Protein Form: Full Length, Recombinant, Active

Target Information

PI4K2B lipid kinase is a member of the phosphatidylinositol 4-kinase family (PI4K) that phosphorylate phosphatidylinositol to generate phosphatidylinositol 4-phosphate which are immediate precursor of several important signaling and scaffolding molecules. PI4K2B is primarily a cytosolic PI4K that is recruited to membranes where it stimulates phosphatidylinositol 4,5-bisphosphate syntheses. PI4K2B uses phosphatidylinositol as its primary substrate and has no activity on phosphatidylinositol monophosphates. PI4K2B is a restricted minor histocompatibility antigen in patients that had been successfully treated with donor lymphocyte infusions for relapsed chronic myeloid leukemia after allogeneic stem cell transplantation. Phosphatidylinositol 4-kinases (PI4Ks) phosphorylate phosphatidylinositol to generate phosphatidylinositol 4-phosphate (PIP), an immediate precursor of several important signaling and scaffolding molecules. PIP itself may also have direct functional and structural roles. PI4K2B is a primarily cytosolic PI4K that is recruited to membranes, where it stimulates phosphatidylinositol 4, 5-bisphosphate synthesis (Wei et al., 2002 ).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: phosphatidylinositol 4-kinase; Phosphatidylinositol 4-kinase type 2-beta; Phosphatidylinositol 4-kinase type II-beta; phosphatidylinositol 4-kinase type-II beta; PI4K2B protein; PI4KII-BETA