Invitrogen

Human PIM3, GST Tag Recombinant Protein

Product Details

A30516

Applications

Tested Dilution

Publications

Kinase Assay (KA)

Assay-dependent

Product Specifications

Species

Human

Expression System

Insect cells

Amino acid sequence

Full length

Tag

GST-tag

Molecular weight

62.6 kDa

Class

Recombinant

Type

Protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

purified

Storage buffer

50mM tris HCl, pH 7.5, with 10mM glutathione, 25% glycerol, 0.1mM PMSF, 0.25mM DTT, 150mM NaCl, 0.1mM EDTA

Contains

no preservative

Storage conditions

-80° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Dry ice

Product Specific Information

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 µg, 10 µg and 20 µg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 µL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase.

This PIM3 recombinant human protein (full length) was expressed in insect cells.

PIM3 recombinant human protein is also available in our SelectScreen™ Kinase Profiling Service.

• Validated for use with the Z'-LYTE™ assay
• Available in multiple pack sizes for convenient use
• Also available in our SelectScreen Kinase Profiling Service

Protein Form: Full Length, Recombinant, Active

Target Information

PIM3 serine/threonine kinase or PIM-3 oncogene belongs to a family of protooncogenes. PIM3 is expressed in hepatocellular carcinoma development and plays a main role in the proliferation of human hepatoma cell lines. PIM3 also expressed in human pancreatic cancer cells and phosphorylates BAD to block Bad-mediated apoptosis in human pancreatic cancer cell lines. PIM3 is widely expressed, with the exception of no expression observed in colon, thymus, and small intestine.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: pim-3 oncogene; serine/threonine kinase Pim-3; Serine/threonine-protein kinase pim-3