RapidFinder™ Quant Multi-Animal Set

The RapidFinder™ Quant Multi-Animal Set, when used with one or more of the RapidFinder™ Animal Species ID kits listed below, enables the determination of the percentage of a target animal species with respect to total animal in food and feed samples. Using the included standards, the kit enables quantification of as little as 0.05% target species DNA as compared to other animal DNA in only a few hours.

The RapidFinder Quant Multi-Animal Set is designed to be used in combination with one or more of the following animal species-specific ID kits (available separately):



• Simple-real-time PCR based assay, no electrophoresis needed
• Flexible-compatible with any food or feed sample, both raw and processed
• Reliable-Includes multi-animal DNA quantification standard
• Fast-from sample to result in the same day

How It Works
The method relies on quantitative real-time PCR. First, a standard curve is created by serially diluting the included multi-animal DNA standard. Then assays for the target species DNA (from one of the kits listed above) and for all animal DNA (from the RapidFinder™ Quant Multi-Animal Set) are run side-by-side on both the food sample DNA and the DNA standards. This approach enables rapid and accurate quantification and reporting of the percentage of target animal species as compared to total animal in a sample. Included in each reaction mix is an internal positive control detected in the VIC™ dye channel, which is used to verify the absence of PCR inhibitors.

The RapidFinder™ Quant Multi-Animal Set is optimized and validated for use with the Applied Biosystems™ QuantStudio™ 5 Real‑Time PCR Instrument and the Applied Biosystems™ 7500 Fast Real‑Time PCR Instrument.

Sample Preparation
DNA sample preparation for the RapidFinder™ animal species identification and quantification workflow is accomplished with the easy-to-use GMO Extraction Kit (Cat. No. 4466336). Using 10 g of sample rather than the smaller sample sizes accommodated by most other DNA isolation procedures minimizes sampling errors. The GMO Extraction Kit is compatible with raw and processed meat, as well as composite food samples.