Invitrogen

TNF alpha Monoclonal Antibody (MAb11), FITC

TNF alpha Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 2

TNF alpha Antibody (A18469) in Flow

Red blood cell lysed peripheral blood mononuclear cells were left unstimulated (left column) or stimulated (right column) for 5 hours with phorbol myristate acetate (PMA) and ionomycin in the presence of brefeldin A. Cells were then fixed and permeabilized using the FIX & PERM® Cell Permeabilization Kit (Cat. no. GAS003 or GAS004) and stained intracellularly with anti-human CD4 APC (Cat. no. MHCD0405) and either anti-human interferon gamma (IFN-gamma) FITC (Cat. no. MHCIFG01) (A) or anti-human tumor necrosis factor alpha (TNF-a) FITC (Cat.... View More

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Product Details

A18469

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-dependent

Product Specifications

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MAb11

Immunogen

human tumor necrosis factor alpha

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.1 mg/mL

Storage conditions

4° C, store in dark

Shipping conditions

Wet ice

RRID

AB_2535320

Target Information

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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