Invitrogen

MHC Class II (I-A/I-E) Monoclonal Antibody (M5/114.15.2), Pacific Blue™

MHC Class II (I-A/I-E) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 1

MHC Class II (I-A/I-E) Antibody (A14901) in Flow

C57BL/6 mouse splenocytes stained with I-A/I-E Rat Anti-Mouse-Pacific Blue™ Monoclonal Antibody (Cat. no. A14901) or a rat IgG2b,κ Pacific Blue™ isotype control (open histogram).

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Product Details

A14901

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-dependent

Product Specifications

Host/Isotype

Rat / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

M5/114.15.2

Immunogen

mouse major histocompatibility complex class II molecules, both I-A and I-E subregion-encoded glycoproteins.

Conjugate

Pacific Blue™ Pacific Blue™ Pacific Blue™

Excitation/Emission Max

404/455 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_2534339

Target Information

MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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