Invitrogen™
GeneArt™ pYES1L Vector with Sapphire™ Technology
GeneArt™ pYES1L Vector with Sapphire Technology™ is a ready-to-use, 9.3 Kb linearized BAC⁄YAC shuttle vector, used for the assembly of Read more
Catalog number A13287
Price (USD)/ 10 reactions
387.65
Online exclusive
423.00 Save 35.35 (8%)
-
Price (USD)/ 10 reactions
387.65
Online exclusive
423.00 Save 35.35 (8%)
GeneArt™ pYES1L Vector with Sapphire™ Technology
Catalog numberA13287
Price (USD)/ 10 reactions
387.65
Online exclusive
423.00 Save 35.35 (8%)
-
GeneArt™ pYES1L Vector with Sapphire Technology™ is a ready-to-use, 9.3 Kb linearized BAC⁄YAC shuttle vector, used for the assembly of DNA fragments with the GeneArt™ High-Order Genetic Assembly System (cat#A13285 & A13286). The vector has the capacity to clone up to 110 Kb of DNA fragments. This product only includes the linearized GeneArt™ pYES1L Vector with Sapphire Technology™.
Some of the key features and elements of the GeneArt™ pYES1L Vector with Sapphire Technology™ include:
• TRP1 to allow selection of yeast transformants in tryptophan-deficient medium
• ARS⁄CEN origin to allow stable maintenance of the construct in yeast
• F’ for conjugation
• oriT for maintenance of large constructs in E.coli
• Spectinomycin resistance for selection in E.coli
• 6 Convenient and validated restriction sites flanking the cloning region
Some of the key features and elements of the GeneArt™ pYES1L Vector with Sapphire Technology™ include:
• TRP1 to allow selection of yeast transformants in tryptophan-deficient medium
• ARS⁄CEN origin to allow stable maintenance of the construct in yeast
• F’ for conjugation
• oriT for maintenance of large constructs in E.coli
• Spectinomycin resistance for selection in E.coli
• 6 Convenient and validated restriction sites flanking the cloning region
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Antibiotic Resistance BacterialSpectinomycin
Cloning MethodSeamless Cloning
Sample TypeDNA
For Use With (Application)Cloning
Number of FragmentsUp to 10 Fragments
Product LineGeneArt™
Quantity10 Reactions
Replication OriginARS⁄CEN, oriT
Selection Marker EukaryoticTRP1
Shipping ConditionDry Ice
Size110 kb total (vector plus all inserts)
VectorpYES1L Linearized
Unit Size10 reactions
Contents & Storage
The product contains 3 vials of linear GeneArt pYES1L Vector with Sapphire Technology at a concentration of 50 ng/mL. Vials contain enough plasmid for 10 cloning reactions using the GeneArt High-Order Genetic Assembly System (needs to be purchased separately).
Store the product at -80°C.
The vector is guaranteed stable for 6 months when properly stored.
Store the product at -80°C.
The vector is guaranteed stable for 6 months when properly stored.