Using StemPro™ MSC SFM, human MSCs can be expanded beyond 5 passages while still maintaining their tri-lineage mesoderm differentiation potential (i.e., ability to differentiate into osteogenic, chondrogenic and adipogenic lineages). Each lot of StemPro™ MSC SFM is qualified using a human MSC performance assay and human MSCs growing in serum-supplemented medium can be transitioned directly into StemPro™ MSC SFM with little or no adaptation required. In addition, StemPro™ MSC SFM facilitates expansion of MSCs directly from primary human bone marrow.