Invitrogen

TMT Monoclonal Antibody (25D5)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

1 Published Figure

7 References

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

FIGURE: 1 / 2

TMT Antibody (90075) in WB

The Biochemical journal 2017 - Published figure using TMT monoclonal antibody (Product # 90075) in Western Blot

Product Image Gallery

TMT Antibody — Figure 7 S-nitrosylation of N. gonnorrhoae GCYH-IB. A) iodoTMT-switch assay of freshly purified wild-type GCYH-IB, of the enzyme after treatment with 0.5 mM GSH, and of the Cys149Ala mutant. Top: Western blot of TMT-labeled proteins visualized using an anti-TMT antibody. Bottom: Coomassie Brilliant Blue staining of the same gel showing equal loading in all lanes. B) Relative H 2 NTP producing activities of the enzyme samples in panel A as measured using the fluorescence assay. The error bars indicate the standard error of triplicate measurements. {TM}

Product Details

90075

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 6 publications 6 publications

Immunoprecipitation (IP)

Assay-dependent

Immunoelectrophoresis (IE)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Chemical

Published species

Chemical, Tag

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

25D5

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Protein G

Storage buffer

PBS with 4% BSA

Contains

Kathon

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10854708

Product Specific Information

Product # 90075 binds to immobilized TMT surface.

Product # 90075 has been succesfully used in immunoprecipitation and Western blot applications.

Target Information

Tandem Mass Tag (TMT) reagents enable concurrent identification and multiplexed quantitation of proteins in different samples using tandem mass spectrometry (MS). Isobaric TMT reagents share identical strucures, including a peptide-reactive labeling group, a spacer arm and a MS/MS reporter. During the MS/MS analysis, each isobaric tag produces a unique reporter ion that makes quantitation possible. Amine-reactive NHS-ester isotopic duplex or six-plex isobaric TMT tags covalently react with amino termini and lysine residues of peptides and proteins. Cysteine-reactive Tandem Mass Tag (cysTMT) reagents react with reduced thiols of cysteine-containing peptides.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Hexokinase 1 cellular localization regulates the metabolic fate of glucose.

Molecular cell

De Jesus A,Keyhani-Nejad F,Pusec CM,Goodman L,Geier JA,Stoolman JS,Stanczyk PJ,Nguyen T,Xu K,Suresh KV,Chen Y,Rodriguez AE,Shapiro JS,Chang HC,Chen C,Shah KP,Ben-Sahra I,Layden BT,Chandel NS,Weinberg SE,Ardehali H

90075 was used in Western Blot to indicate that HK1 mitochondrial binding alters glucose metabolism through regulation of GAPDH.

Thu Apr 07 00:00:00 EDT 2022

Nuclear S-nitrosylation impacts tissue regeneration in zebrafish.

Nature communications

Matrone G,Jung SY,Choi JM,Jain A,Leung HE,Rajapakshe K,Coarfa C,Rodor J,Denvir MA,Baker AH,Cooke JP

90075 was used in Western Blot to provide evidence that inducible nitric oxide synthase (iNos) translocates to the nucleus during zebrafish tailfin regeneration and is associated with alterations in the nuclear S-nitrosylated proteome.

Mon Nov 01 00:00:00 EDT 2021

Helicase-like transcription factor-deletion from the tumor microenvironment in a cell line-derived xenograft model of colorectal cancer reprogrammed the human transcriptome-S-nitroso-proteome to promote inflammation and redirect metastasis.

PloS one

Helmer RA,Martinez-Zaguilan R,Kaur G,Smith LA,Dufour JM,Chilton BS

90075 was used in Immunoelectrophoresis to conclude that Hltf-deletion from the TME played a major role in the pathogenesis of inflammation and linked protein S-nitrosylation in primary CDX tumors with spatiotemporal continuity in metastatic progression when the tumor cells expressed HLTF.

Wed Oct 20 00:00:00 EDT 2021

Inhibition of mitochondrial fission and iNOS in the dorsal vagal complex protects from overeating and weight gain.

Molecular metabolism

Patel B,New LE,Griffiths JC,Deuchars J,Filippi BM

90075 was used in Western Blotting to uncover new molecular and cellular targets for brain regulation of whole-body metabolism, which could inform new strategies to combat obesity and diabetes.

Fri Jan 01 00:00:00 EST 2021

Corticotropin-releasing hormone (CRH) alters mitochondrial morphology and function by activating the NF-kB-DRP1 axis in hippocampal neurons.

Cell death & disease

Battaglia CR,Cursano S,Calzia E,Catanese A,Boeckers TM

90075 was used in Western Blot to investigate the direct involvement of mitochondria in the (mal)-adaptive changes induced by the activation of neuronal CRH receptors 1 (CRHR1).

Mon Nov 23 00:00:00 EST 2020

Impact of inflammation on brain subcellular energetics in anesthetized rats.

BMC neuroscience

Thiele RH,Osuru HP,Paila U,Ikeda K,Zuo Z

90075 was used in Western Blotting to demonstrate that, after induction of inflammation, exposure to isoflurane increases hypoxia-inducible factor-1α, oxidative stress in the brain and serum lactate in rats.

Mon Jul 15 00:00:00 EDT 2019

Mechanism and catalytic strategy of the prokaryotic-specific GTP cyclohydrolase-IB.

The Biochemical journal

Paranagama N,Bonnett SA,Alvarez J,Luthra A,Stec B,Gustafson A,Iwata-Reuyl D,Swairjo MA

Published figure using TMT monoclonal antibody (Product # 90075) in Western Blot

Tue Mar 07 00:00:00 EST 2017

Bioinformatics

Protein Aliases: Tandem Mass Tag

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