Pierce™ Polyacrylamide Spin Desalting Columns, 7K MWCO, 0.7 mL
Pierce™ Polyacrylamide Spin Desalting Columns, 7K MWCO, 0.7 mL
Thermo Scientific™

Pierce™ Polyacrylamide Spin Desalting Columns, 7K MWCO, 0.7 mL

Separate proteins and other macromolecules from low-molecular weight buffer salts and reagents with Thermo Scientific Pierce 7K MWCO (molecular-weight cutoff) Polyacrylamide Spin Desalting Columns, which are porous polyacrylamide-based gel-filtration columns for biological sample preparation.
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Catalog NumberMWCOQuantitySample Volume (Metric)
898497.0 kDa25 Columns30 to 120 μL
898627.0 kDa50 Columns30 to 120 μL
Catalog number 89849
Price (USD)/ 25 columns
204.65
Online exclusive
209.00 
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MWCO:
7.0 kDa
Quantity:
25 Columns
Sample Volume (Metric):
30 to 120 μL
Request bulk or custom format
Price (USD)/ 25 columns
204.65
Online exclusive
209.00 
Save 4.35 (2%)
Add to cart
Pierce™ Polyacrylamide Spin Desalting Columns, 7K MWCO, 0.7 mL
Catalog number89849
Price (USD)/ 25 columns
204.65
Online exclusive
209.00 
Save 4.35 (2%)
-
Add to cart
Desalting and buffer exchange are two of the most widely used applications in resin filtration chromatography. Thermo Scientific Pierce Polyacrylamide Spin Desalting Columns, 7K MWCO (molecular-weight cutoff), are ready-to-use, disposable, gel-filtration columns for the separation of proteins and other macromolecules from low-molecular-weight buffer salts and reagents.

Pierce Polyacrylamide Spin Desalting Columns, 7K MWCO, are pre-packed with porous polyacrylamide beads and enable buffer-exchange and desalting of proteins and other macromolecular samples based on a molecular-weight cutoff (MWCO) of 7000 Da. Unlike other size-exclusion media, polyacrylamide resin is not subject to enzymatic degradation and will not serve as a nutrient for microbial growth. The resin is very hydrophilic, thereby minimizing undesirable binding interactions between the resin and sample molecules. Contamination with low molecular weight sugars (which may occur with crosslinked dextran resin) is not a concern when using polyacrylamide resin. Oxidizing agents can be removed without destroying the support. This resin is susceptible to hydrolysis of amide groups under extreme pH conditions, so an operating pH of 2–10 is recommended at room temperature. The polyacrylamide resin can also be autoclaved at pH 5.5–6.5 for 30 minutes at 120°C.

The columns use a resin with beads that have a wet diameter of 45 to 90 μM and are ideal for separating peptides and small macromolecules (>7 kDa) from buffer salts and other compounds (less than 1K MWCO).

Features of polyacrylamide resin include:
• Stable in water, salt solutions, organic solvents, and alkaline or acidic conditions
• Excellent flow properties
• Heat stable

Applications
• Removing salts from protein solutions
• Removing phenol from nucleic acid preparations
• Separating excess crosslinker from conjugate preparations
• Removing excess derivatizing agents from modified proteins
• Removing unreacted dye from fluorescent antibodies
• Removing free radiolabel from labeled proteins
• Buffer exchange

For Research Use Only. Not for use in diagnostic procedures.
Gravity-flow gel filtration involves the chromatographic separation of molecules of different dimensions based on their relative abilities to penetrate into a suitable stationary phase. A chromatographic matrix, usually consisting of very small, uncharged porous particles in an aqueous solution, is packed into a column and then used for the separation. Different levels of separation can be achieved based on the pore size of the medium packed into the column. The medium can be chosen to totally exclude proteins or large molecules, while still including small solutes. Large molecules are excluded from the internal pores of the gel and emerge first from the column. The smaller molecules are able to penetrate the pores and then progress through the column at a slower rate. These smaller molecules are subsequently flushed through the column with additional buffer volume.
Specifications
DescriptionPierce™ Polyacrylamide Spin Desalting Columns, 7K MWCO, 0.7 mL
Product TypeSpin Desalting Column
FormatSpin Column
Purification TargetBuffer Exchange, Protein
Column TypeSize-exclusion, Polyacrylamide Resin
MWCO7.0 kDa
Product LinePierce™
Quantity25 Columns
Sample Volume (Metric)30 to 120 μL
Unit Size25 columns
Contents & Storage
Upon receipt store at 4°C.