Thermo Scientific Powdered DMEM Medium for SILAC is optimized for use with stable isotope labeling with amino acids in cellRead more
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Catalog number 88425
Price (USD)/ 135 g
352.00
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Request bulk or custom format
Price (USD)/ 135 g
352.00
Add to cart
Powdered DMEM Medium for SILAC
Catalog number88425
Price (USD)/ 135 g
352.00
-
Add to cart
Thermo Scientific Powdered DMEM Medium for SILAC is optimized for use with stable isotope labeling with amino acids in cell culture (SILAC) to analyze protein expression by mass spectrometry (MS).
Features of Powdered DMEM Medium for SILAC:
• Flexible—powdered medium deficient in L-leucine, L-lysine and L-arginine, allowing for more complete proteome coverage through multiple isotopic amino acid labeling • High quality—medium is sterile, endotoxin-free and cell culture-compatible
Stable isotope labeling with amino acids in cell culture (SILAC) is a powerful method to identify and quantify relative differential changes in complex protein samples. This approach entails the in vivo metabolic incorporation of 'heavy' 13C- or 15N-labeled amino acids into proteins followed by mass spectrometry (MS) for the accelerated and comprehensive identification, characterization and quantitation of proteins.
This SILAC medium is used together with heavy and light amino acids for the differential isotopic labeling of cells for SILAC analysis. SILAC medium supplemented with heavy amino acids are capable of nearly 100% label incorporation into proteins of living cells.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
FormulationDMEM media minus L-Leucine, L-Lysine and L-Arginine