Thermo Scientific™
SuperSignal™ Molecular Weight Protein Ladder
Thermo Scientific SuperSignal Molecular Weight Protein Ladder (20 to 150K) is a mix of eight unstained recombinant proteins with IgG-binding Read more
Catalog number 84785
Price (USD)/ 250 µL
305.65
Online exclusive
320.00 Save 14.35 (4%)
Price (USD)/ 250 µL
305.65
Online exclusive
320.00 Save 14.35 (4%)
SuperSignal™ Molecular Weight Protein Ladder
Catalog number84785
Price (USD)/ 250 µL
305.65
Online exclusive
320.00 Save 14.35 (4%)
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Thermo Scientific SuperSignal Molecular Weight Protein Ladder (20 to 150K) is a mix of eight unstained recombinant proteins with IgG-binding sites for chemiluminescent, fluorescent, chromogenic or other detection systems. The protein standard is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.
Compare and view all other protein standards and ladders ›
The ladder’s recombinant proteins bind to the antibodies used in the western blot through the IgG binding site. The protein markers can then be visualized using appropriate substrates for enzyme-labeled antibodies or via fluorescent dye-labeled antibodies (not recommended for antibodies labeled with fluors in the 500–550 nm channel).
Two versions of the MW marker are offered. This formulation is appropriate for use with most rabbit and other non-mouse polyclonal antibodies. SuperSignal Enhanced Molecular Weight Protein ladder is formulated specifically for applications requiring mouse monoclonal antibodies and experiments where very dilute antibody concentrations are used. For example, the enhanced standard may be necessary when using a 1:50,000 (25 ng/mL) to 1:100,000 (10 ng/mL) dilution of secondary antibody.
Compare and view all other protein standards and ladders ›
The ladder’s recombinant proteins bind to the antibodies used in the western blot through the IgG binding site. The protein markers can then be visualized using appropriate substrates for enzyme-labeled antibodies or via fluorescent dye-labeled antibodies (not recommended for antibodies labeled with fluors in the 500–550 nm channel).
Two versions of the MW marker are offered. This formulation is appropriate for use with most rabbit and other non-mouse polyclonal antibodies. SuperSignal Enhanced Molecular Weight Protein ladder is formulated specifically for applications requiring mouse monoclonal antibodies and experiments where very dilute antibody concentrations are used. For example, the enhanced standard may be necessary when using a 1:50,000 (25 ng/mL) to 1:100,000 (10 ng/mL) dilution of secondary antibody.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodUser defined detection system
Number of Markers8
Product TypeProtein Ladder
Ready to LoadYes
Size Range20 to 150 kDa
Stain TypeUnstained
Gel CompatibilityBolt™ Bis-Tris Plus Gels, Novex™ Tricine Gels, Novex™ Tris-Glycine Gels, NuPAGE™ Bis-Tris Gels, NuPAGE™ Tris-Acetate Gels, SDS-PAGE Gels
Molecular Weight (g/mol)150, 100, 80, 60, 50, 40, 30, 20 kDa
Product LineSuperSignal™
Quantity250 μL
Shipping ConditionWet Ice
System TypeWestern Blotting, SDS-PAGE
Unit Size250 µL
Contents & Storage
Contents: one vial of 250 μL
Storage buffer: 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3 and 33% glycerol
Storage: Upon receipt store at -20°C for long term storage, or three months at 4°C or one month at ambient
Storage buffer: 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3 and 33% glycerol
Storage: Upon receipt store at -20°C for long term storage, or three months at 4°C or one month at ambient