ExoSAP-IT™ PCR Product Cleanup Reagent
| Catalog Number | No. of Reactions |
|---|---|
| 78201.1.ML | 500 Reactions |
| 78205.10.ML | 5000 Reactions |
| 78202.4X.1.ML | 2000 Reactions |
| 78200.200.UL | 100 Reactions |
ExoSAP-IT™ PCR Product Cleanup Reagent is used for enzymatic cleanup of amplified PCR product. It hydrolyzes excess primers and nucleotides in a single step. ExoSAP-IT-purified samples are ready for use in downstream applications such as DNA sequencing or single nucleotide polymorphism (SNP) analysis.
- Remove excess primers and unincorporated nucleotides enzymatically
- Prepare DNA sequencing samples with one pipetting step
- Conserve precious PCR products with up to 100% recovery
- Minimize column cleanup and sample loss
The ExoSAP-IT one-step solution provides a simple way to enzymatically purify a PCR reaction. The reaction setup is complete with one pipetting step, which is followed by two incubations. The first incubation digests excess primer and dephosphorylates nucleotides. The second, high-temperature incubation inactivates the enzymes.
Enzymatic cleanup is a very efficient method for PCR purification. Adding ExoSAP-IT reagent directly to the PCR product eliminates transfer steps to tubes, wells, or columns and conserves PCR amplicons, helping reduce the chance of cross contamination; no further processing needed.
After enzymatic cleanup with ExoSAP-IT reagent, primers and dNTPs will no longer interfere with DNA sequencing or SNP analysis.