Invitrogen

Phospho-Rb (Thr821) Recombinant Superclonal Antibody (16HCLC)

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Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

710314

Applications
Tested Dilution
Publications

Western Blot (WB)

1-3 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

1:20
-

Immunocytochemistry (ICC/IF)

1-2 µg/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Superclonal

Type

Antibody

Clone

16HCLC

Immunogen

Phosphopeptide corresponding to amino acids 817-825 of human Retinoblastoma [pT821]
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2532682

Product Specific Information

This antibody is predicted to react with mouse, rat, non-human primate and rabbit based on sequence homology.

Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.

Target Information

Rb is a tumor suppressor nuclear phosphoprotein capable of binding to DNA. It is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus-16 E. Rb protein may act by regulating transcription and loss of its function leads to uncontrolled cell growth. Aberrations in the RB gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Cleaved Rb; delta RB-p70; DRb-p70; exon 17 tumor GOS561 substitution mutation causes premature stop; GOS563 exon 17 substitution mutation causes premature stop; p105-Rb; P105RB; p110-RB1; Phospho-Retinoblastoma; pp110; pRb; prepro-retinoblastoma-associated protein; protein phosphatase 1, regulatory subunit 130; Rb; Rb-p70; Retin; Retinoblastoma; retinoblastoma 1; retinoblastoma gene; retinoblastoma suspectibility protein; Retinoblastoma-associated protein

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Gene Aliases: OSRC; p105-Rb; pp110; PPP1R130; pRb; RB; RB1

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UniProt ID: (Human) P06400

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Entrez Gene ID: (Human) 5925

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Function(s)
core promoter binding RNA polymerase II activating transcription factor binding DNA binding transcription factor activity, sequence-specific DNA binding transcription coactivator activity protein binding transcription factor binding kinase binding ubiquitin protein ligase binding identical protein binding androgen receptor binding phosphoprotein binding chromatin/chromatin-binding, or -regulatory protein
Process(es)
cell cycle checkpoint G1/S transition of mitotic cell cycle regulation of transcription involved in G1/S transition of mitotic cell cycle chromatin remodeling transcription, DNA-templated negative regulation of protein kinase activity cell cycle arrest negative regulation of transcription from RNA polymerase II promoter during mitosis mitotic cell cycle checkpoint Ras protein signal transduction regulation of mitotic cell cycle negative regulation of gene expression viral process covalent chromatin modification androgen receptor signaling pathway sister chromatid biorientation neuron projection development maintenance of mitotic sister chromatid cohesion glial cell apoptotic process skeletal muscle cell differentiation neuron maturation enucleate erythrocyte differentiation negative regulation of sequence-specific DNA binding transcription factor activity regulation of lipid kinase activity myoblast differentiation positive regulation of macrophage differentiation positive regulation of mitotic metaphase/anaphase transition negative regulation of smoothened signaling pathway negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter digestive tract development cell morphogenesis involved in neuron differentiation negative regulation of epithelial cell proliferation striated muscle cell differentiation cell division neuron apoptotic process protein localization to chromosome, centromeric region cellular response to xenobiotic stimulus regulation of cohesin loading negative regulation of transcription involved in G1/S transition of mitotic cell cycle regulation of centromere complex assembly hepatocyte apoptotic process negative regulation of G1/S transition of mitotic cell cycle positive regulation of transcription regulatory region DNA binding
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