Invitrogen

Phospho-PYK2 (Tyr402) Recombinant Superclonal Antibody (17HCLC)

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View all (41) PYK2 antibodies
Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

710087

Applications
Tested Dilution
Publications

Western Blot (WB)

4-5 µg/mL
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Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100
-
Product Specifications

Species Reactivity

Human

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Superclonal

Type

Antibody

Clone

17HCLC

Immunogen

proprietary

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2532553

Product Specific Information

This antibody is predicted to react with mouse based on sequence homology.

Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.

Target Information

PTK2B (FAK2) is a cytoplasmic tyrosine kinase involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. PTK2B regulates reorganization of the actin cytoskeleton, cell polarization, cell migration, adhesion, spreading, and bone remodeling. FAK2 plays a role in the regulation of the humoral immune response and is required for normal levels of marginal B-cells in the spleen and normal migration of splenic B-cells. It functions in signaling downstream of integrin and collagen receptors, immune receptors, G-protein coupled receptors, cytokine, chemokine, and growth factor receptors, and mediates responses to cellular stress. Aberrant FAK2 expression may play a role in cancer cell proliferation, migration, and invasion in tumor formation and metastasis. Elevated expression has been seen in gliomas, hepatocellular carcinoma, lung cancer, and breast cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CADTK; CAK-beta; Calcium-dependent tyrosine kinase; Calcium-regulated non-receptor proline-rich tyrosine kinase; Cell adhesion kinase beta; FADK; FADK 2; FAK; FAK1; Focal adhesion kinase 2; Proline-rich tyrosine kinase 2; protein kinase B; Protein-tyrosine kinase 2-beta; PTK2B protein tyrosine kinase 2 beta; RAFTK; Related adhesion focal tyrosine kinase

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Gene Aliases: CADTK; CAKB; FADK2; FAK2; PKB; PTK; PTK2B; PYK2; RAFTK

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UniProt ID: (Human) Q14289

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Entrez Gene ID: (Human) 2185

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Function(s)
calmodulin-dependent protein kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity signal transducer activity NMDA glutamate receptor activity receptor binding protein binding ATP binding protein complex binding 3-phosphoinositide-dependent protein kinase binding
Process(es)
MAPK cascade angiogenesis oocyte maturation response to hypoxia positive regulation of cell-matrix adhesion sprouting angiogenesis adaptive immune response marginal zone B cell differentiation protein complex assembly protein phosphorylation apoptotic process response to stress cellular defense response response to osmotic stress signal transduction cell surface receptor signaling pathway signal complex assembly epidermal growth factor receptor signaling pathway positive regulation of cytosolic calcium ion concentration integrin-mediated signaling pathway positive regulation of cell proliferation negative regulation of cell proliferation regulation of cell shape response to mechanical stimulus response to hormone response to glucose response to lithium ion positive regulation of endothelial cell migration negative regulation of muscle cell apoptotic process regulation of cGMP-mediated signaling regulation of macrophage chemotaxis positive regulation of neuron projection development glial cell proliferation peptidyl-tyrosine phosphorylation regulation of cell adhesion positive regulation of cell growth positive regulation of cell migration negative regulation of bone mineralization regulation of cGMP biosynthetic process positive regulation of actin filament polymerization neuron projection development regulation of inositol trisphosphate biosynthetic process tumor necrosis factor-mediated signaling pathway ionotropic glutamate receptor signaling pathway response to immobilization stress peptidyl-tyrosine autophosphorylation response to cocaine response to drug response to hydrogen peroxide activation of Janus kinase activity negative regulation of apoptotic process stress fiber assembly negative regulation of potassium ion transport positive regulation of JUN kinase activity negative regulation of neuron apoptotic process blood vessel endothelial cell migration positive regulation of phosphatidylinositol 3-kinase activity innate immune response positive regulation of nitric oxide biosynthetic process bone resorption response to ethanol negative regulation of myeloid cell differentiation positive regulation of translation positive regulation of angiogenesis positive regulation of protein kinase activity positive regulation of JNK cascade protein autophosphorylation vascular endothelial growth factor receptor signaling pathway focal adhesion assembly positive regulation of peptidyl-tyrosine phosphorylation regulation of calcium-mediated signaling positive regulation of nitric-oxide synthase activity regulation of release of sequestered calcium ion into cytosol response to cAMP response to calcium ion positive regulation of synaptic transmission, glutamatergic long-term synaptic potentiation long term synaptic depression chemokine-mediated signaling pathway positive regulation of ERK1 and ERK2 cascade cellular response to retinoic acid cellular response to fluid shear stress activation of GTPase activity regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process regulation of establishment of cell polarity regulation of actin cytoskeleton reorganization regulation of N-methyl-D-aspartate selective glutamate receptor activity positive regulation of excitatory postsynaptic potential positive regulation of B cell chemotaxis positive regulation of DNA biosynthetic process
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