Invitrogen

K-Ras Recombinant Rabbit Monoclonal Antibody (11H35L14)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

3 References

View all (50) K-Ras antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

FIGURE: 1 / 5

K-Ras Antibody (703345) in WB

Western blot analysis was performed on whole cell extracts (30 µg lysate) of HCT 116 (Lane 1), T-47D (Lane 2), SK-BR-3 (Lane 3), Caco-2 (Lane 4), HT-29 (Lane 5), SW480 (Lane 6) and PANC-1 (Lane 7). The blot was probed with Anti-K-Ras Recombinant Rabbit Monoclonal Antibody (Product # 703345, 1:5000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution). A ~21 kDa band corresponding to K-Ras was observed across the cell lines test... View More

Product Image Gallery

K-Ras Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of the target protein. HCT 116 cells were transfected with K-Ras siRNA and a decrease in signal intensity was observed in Western blot application using Anti-K-Ras Recombinant Rabbit Monoclonal Antibody (Product # 703345). {KD}

Product Details

703345

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

11H35L14

Immunogen

Peptides corresponding to human K-Ras [(aa160-aa177) (aa88-105)]

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2784575

Product Specific Information

This antibody is predicted to react with Monkey, Pig, Rat.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

K-RAS (GTPase KRas) belongs to the Ras oncogene family. Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. It plays an important role in the regulation of cell proliferation and has a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes in colorectal cancer cells in a ZNF304-dependent manner. Mutations in the gene can result in leukemia acute myelogenous, leukemia juvenile myelomonocytic, Noonan Syndrome, gastric cancer, and cardiofaciocutaneous syndrome 2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CRISPR/Cas9 Gene Editing of HeLa Cells to Tag Proteins with mNeonGreen.

Bio-protocol

Surve S,Sorkin A

703345 was used in Western Blotting to describe methodological approaches to label components of the EGFR-RAS-MAPK pathway, such as Grb2, KRAS, and NRAS, with the fluorescent protein mNeonGreen (mNG) using CRISPR/Cas9 gene-editing, as well as generation of homozygous single-cell clones of the edited target protein.

Fri May 20 00:00:00 EDT 2022

KARATE: PKA-induced KRAS4B-RHOA-mTORC2 supercomplex phosphorylates AKT in insulin signaling and glucose homeostasis.

Molecular cell

Senoo H,Murata D,Wai M,Arai K,Iwata W,Sesaki H,Iijima M

703345 was used in Western Blotting to reveal a fundamental mechanism that activates mTORC2 toward AKT in insulin-regulated glucose homeostasis.

Thu Nov 18 00:00:00 EST 2021

EGFR-RAS-MAPK signaling is confined to the plasma membrane and associated endorecycling protrusions.

The Journal of cell biology

Surve S,Watkins SC,Sorkin A

703345 was used in Immunocytochemistry-immunofluorescence to propose that a small pool of surface EGFRs sustain signaling within the RAS-ERK1/2 pathway and that RAS activation persists in TERCs, whereas endosomal EGFR does not significantly contribute to ERK1/2 activity.

Mon Nov 01 00:00:00 EDT 2021

Bioinformatics

Protein Aliases: c-K-ras; c-Ki-ras; c-Kirsten-ras protein; cellular c-Ki-ras2; cellular c-Ki-ras2 proto-oncogene; GTPase KRas; K RAS; K-Ras 2; K-ras p21 protein; Ki-Ras; Kirsten rat sarcoma viral oncogene homolog; oncogene KRAS2; PR310 c-K-ras oncogene; RASK; transforming protein p21; v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog

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Gene Aliases: C-K-RAS; CFC2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KI-RAS; KRAS; KRAS1; KRAS2; NS; NS3; RALD; RASK2

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UniProt ID: (Human) P01116

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Entrez Gene ID: (Human) 3845

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Function(s)

GTPase activity protein binding GTP binding GMP binding GDP binding LRR domain binding protein complex binding small GTPase

Process(es)

MAPK cascade positive regulation of protein phosphorylation stimulatory C-type lectin receptor signaling pathway epidermal growth factor receptor signaling pathway Ras protein signal transduction axon guidance positive regulation of cell proliferation visual learning positive regulation of gene expression cytokine-mediated signaling pathway forebrain astrocyte development actin cytoskeleton organization regulation of protein stability regulation of synaptic transmission, GABAergic positive regulation of Rac protein signal transduction social behavior endocrine signaling Fc-epsilon receptor signaling pathway ERBB2 signaling pathway positive regulation of MAP kinase activity negative regulation of neuron apoptotic process negative regulation of cell differentiation regulation of long-term neuronal synaptic plasticity homeostasis of number of cells within a tissue leukocyte migration positive regulation of nitric-oxide synthase activity positive regulation of NF-kappaB transcription factor activity striated muscle cell differentiation response to glucocorticoid response to mineralocorticoid epithelial tube branching involved in lung morphogenesis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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K-Ras Recombinant Rabbit Monoclonal Antibody (11H35L14)

K-Ras Antibody (703345) in WB

Product Details

703345

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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