Invitrogen

STING Recombinant Rabbit Monoclonal Antibody (2H1L5)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

View all (93) STING antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

STING Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 8

STING Antibody (702993) in ICC/IF

Immunofluorescence of STING1 was performed using PMA-treated THP-1 WT and STING1 KO cells that were labeled with a green or a far-red fluorescent dye, respectively. Post-labeling, WT and KO cells were mixed and plated to a 1:1 ratio in a 96-well plate with an optically clear flat bottom as a mosaic and incubated for 24 hrs. Cells were fixed in 4% PFA (in PBS) for 15 min at RT; cells were permeabilized with 0.1% Triton X-100 for 10 min at RT and blocked with PBS containing 5% BSA, 5% goat serum, and 0.01% Triton X-100 for 30 min at RT. Cell... View More

Product Image Gallery

STING Antibody — Altered expression of the protein upon cell treatment demonstrates antibody specificity. Western blot using Anti-STING Recombinant Rabbit Monoclonal Antibody (Product # 702993, 1:5000 dilution), shows increased expression of protein STING in THP-1 cells upon treatment with 2’3’-cGAMP. {TM}

Product Details

702993

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000

Immunocytochemistry (ICC/IF)

1:250

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

2H1L5

Immunogen

Protein corresponding to human STING [aa139-aa379]

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2762404

Product Specific Information

This antibody is predicted to react with Monkey, Goat, Sheep.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

STING is a 379 amino acid containing signaling protein that acts as an important component of innate immune signaling. This signaling protein is able to activate both NF-kappa-B and IRF3 transcription pathways to induce expression of type I interferon (IFN-alpha and IFN-beta) and exert a potent anti-viral state following expression. STING may have translocon function, the translocon possibly being able to influence the induction of type I interferons and is also involved in transduction of apoptotic signals via its association with the major histocompatibility complex class II (MHC-II) thus facilitating the detection of intracellular viral RNA species as well as B-form DNA and mediates death signaling via activation of the extracellular signal-regulated kinase (ERK) pathway. Generally seen in endoplasmic reticulum membrane, cell membrane and mitochondrion outer membrane, it is ubiquitously expressed in most of tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: endoplasmic reticulum IFN stimulator; Endoplasmic reticulum interferon stimulator; ERIS; hMITA; hSTING; Mediator of IRF3 activation; mitochondrial mediator of IRF3 activation; N-terminal methionine-proline-tyrosine-serine plasma membrane tetraspanner; Stimulator of interferon genes protein; tm173; Transmembrane protein 173

View more View less

Gene Aliases: ERIS; hMITA; hSTING; MITA; MPYS; NET23; SAVI; STING; STING1; TMEM173

View more View less

UniProt ID: (Human) Q86WV6

View more View less

Entrez Gene ID: (Human) 340061

View more View less

Function(s)

protein binding transcription factor binding protein kinase binding ubiquitin protein ligase binding cyclic-di-GMP binding identical protein binding protein homodimerization activity cyclic-GMP-AMP binding

Process(es)

activation of innate immune response positive regulation of defense response to virus by host apoptotic process positive regulation of protein binding regulation of type I interferon production positive regulation of type I interferon production interferon-beta production positive regulation of protein import into nucleus, translocation cellular response to interferon-beta positive regulation of transcription factor import into nucleus innate immune response positive regulation of transcription from RNA polymerase II promoter defense response to virus cellular response to exogenous dsRNA cellular response to organic cyclic compound

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

STING Recombinant Rabbit Monoclonal Antibody (2H1L5)

STING Antibody (702993) in ICC/IF

Product Details

702993

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Species Reactivity

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!