Invitrogen

Glutaminase Recombinant Rabbit Monoclonal Antibody (6H5L15)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

1 Published Figure

4 References

View all (24) Glutaminase antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Glutaminase Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 5

Glutaminase Antibody (701965) in ICC/IF

Knockdown of GLS was achieved by transfecting Hep G2 cells with GLS specific siRNAs (Silencer® select Product # s5839, s5840). Immunofluorescence analysis was performed using untransfected Hep G2 cells (panels a, d), transfected with non-specific scrambled siRNA (panels b,e) and transfected with GLS specific siRNAs (panel c,f). Cells were fixed, permeabilized, and probed with GLS (6H5L15), Recombinant Rabbi... View More

Glutaminase Antibody in Western Blot (WB)

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Product Details

701965

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

2 µg/mL

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

6H5L15

Immunogen

Protein corresponding to Human GLS (aa 251-533)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2633041

Product Specific Information

This antibody is predicted to react with Monkey, Rabbit, Rat and Mouse.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Glutaminase is a phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. It is primarily expressed in the brain and kidney and plays an essential role in generating energy for metabolism, synthesizing neurotransmitter glutamate, and maintaing acid-based balance in the kidney. There are alternate splicing results in multiple transcript variants in the Glutaminase gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CD40 signal rewires fatty acid and glutamine metabolism for stimulating macrophage anti-tumorigenic functions.

Nature immunology

Liu PS,Chen YT,Li X,Hsueh PC,Tzeng SF,Chen H,Shi PZ,Xie X,Parik S,Planque M,Fendt SM,Ho PC

701965 was used in Western Blotting to show that metabolic reprogramming, which includes FAO and glutamine metabolism, controls the activation of pro-inflammatory and anti-tumorigenic polarization, and highlight a therapeutic potential of metabolic preconditioning of tumor-associated macrophages before agonistic anti-CD40 treatments.

Wed Mar 01 00:00:00 EST 2023

Hypoxia Induces Saturated Fatty Acids Accumulation and Reduces Unsaturated Fatty Acids Independently of Reverse Tricarboxylic Acid Cycle in L6 Myotubes.

Frontiers in endocrinology

Vacek L,Dvorak A,Bechynska K,Kosek V,Elkalaf M,Trinh MD,Fiserova I,Pospisilova K,Slovakova L,Vitek L,Hajslova J,Polak J

701965 was used in Western Blotting to investigate changes in lipidomic profile, 13C appearance in rTCA-related metabolites, gene and protein expression of rTCA-related proteins and glutamine transporters, glucose uptake and lactate production.

Mon Apr 04 00:00:00 EDT 2022

CRISPR screens in physiologic medium reveal conditionally essential genes in human cells.

Cell metabolism

Rossiter NJ,Huggler KS,Adelmann CH,Keys HR,Soens RW,Sabatini DM,Cantor JR

701965 was used in Western Blotting to reveal the profound impact of medium composition on gene essentiality in human cells, and also suggest general strategies for using genetic screens in HPLM to uncover new cancer vulnerabilities and gene-nutrient interactions.

Tue Jun 01 00:00:00 EDT 2021

Mitochondrial peptides modulate mitochondrial function during cellular senescence.

Aging

Kim SJ,Mehta HH,Wan J,Kuehnemann C,Chen J,Hu JF,Hoffman AR,Cohen P

Published figure using Glutaminase monoclonal antibody (Product # 701965) in Western Blot

Sun Jun 10 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: DKFZp686O15119; FLJ10358; GLS; glutaminase C; Glutaminase kidney isoform, mitochondrial; glutaminase, phosphate-activated; K-glutaminase; L-glutamine amidohydrolase

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Gene Aliases: 6330442B14; AAD20; AI314027; B230365M23Rik; GAC; GAM; GLS; GLS1; Glut; KGA; KIAA0838; RATGLUT

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UniProt ID: (Human) O94925, (Mouse) D3Z7P3, (Rat) P13264

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Entrez Gene ID: (Human) 2744, (Mouse) 14660, (Rat) 24398

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Function(s)

glutaminase activity protein binding hydrolase activity hydrolase

Process(es)

suckling behavior regulation of respiratory gaseous exchange by neurological system process glutamate biosynthetic process glutamine catabolic process chemical synaptic transmission cellular amino acid biosynthetic process glutamate secretion protein homotetramerization glutamine metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Glutaminase Recombinant Rabbit Monoclonal Antibody (6H5L15)

Glutaminase Antibody (701965) in ICC/IF

Product Details

701965

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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