Invitrogen

FOXA2 Recombinant Rabbit Monoclonal Antibody (9H5L7)

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

3 References

View all (33) FOXA2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

FOXA2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 7

FOXA2 Antibody (701698) in ICC/IF

Immunofluorescence was performed on methanol fixed HepG2 cells for detection of FOXA2 using FOXA2 Recombinant Rabbit Monoclonal Antibody (Product # 701698, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Cytoskeleton was stained with alpha-Tubulin Monoclonal Antibody (Product # 32-2500, 1 µg/mL) followed by Goat anti-Mouse IgG Secondary Antibody, Alexa Fluor® 594 conjugate (Product # A-11032, 1:400). Panel a) shows representative cells that... View More

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FOXA2 Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. A loss of signal was observed for target protein inFOXA2 KO cell line compared to control cell line using FOXA2 Recombinant Rabbit Monoclonal Antibody (9H5L7) (Product # 701698). {KO}

Product Details

701698

Applications

Tested Dilution

Publications

Western Blot (WB)

1:250-1:500

Immunocytochemistry (ICC/IF)

1:100

View 3 publications 3 publications

ChIP assay (ChIP)

2-3 µg/1x10^6 cells

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

9H5L7

Immunogen

Protein corresponding to Human FOXA2 (aa 236-451)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2576439

Product Specific Information

This antibody is predicted to react with Monkey, Horse, Cat and Dog.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

FOXA2 is a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific genes such as albumin and transthyretin. They also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. FOXA2 has been linked to sporadic cases of maturity-onset diabetes of the young. Transcript variants encoding different isoforms have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Generation of two human iPSC lines with Exon 3 mutations in BCL2-Associated Athanogene 3 (BAG3) from dilated cardiomyopathy patients.

Stem cell research

Zushin PH,Zhou Y,Li A,Ashley EA,Wheeler MT,Wu JC

701698 was used in Immunocytochemistry to generate two human iPSC lines from individuals with mutations in exon 3 of BAG3 and provide validation of their pluripotency and ability to differentiate toward the three primary germ layers.

Wed Mar 01 00:00:00 EST 2023

Generation of two induced pluripotent stem cell lines from dilated cardiomyopathy patients caused by heterozygous mutations in the HCN4 gene.

Stem cell research

Yildirim Z,Kojic A,Yan CD,Wu MA,Vagelos R,Wu JC

701698 was used in Immunocytochemistry-immunofluorescence to generate two human induced pluripotent stem cell (iPSC) lines generated from two DCM patients carrying variants in the HCN4 gene (c.2587G > T and c.2846G > A).

Thu Dec 01 00:00:00 EST 2022

Generation of three induced pluripotent stem cell lines to model and investigate diseases affecting Hispanics.

Stem cell research

Chen IY,Olshausen J,Thomas D,Lai C,McLaughlin TL,Wu JC

701698 was used in Immunocytochemistry to generate three induced pluripotent stem cell (iPSC) lines from the peripheral blood mononuclear cells (PBMCs) of healthy Hispanic subjects.

Thu Dec 01 00:00:00 EST 2022

Bioinformatics

Protein Aliases: FKHL20; Forkhead box protein A2; hepatic nuclear factor-3-beta; hepatocyte nuclear factor 3, beta; Hepatocyte nuclear factor 3-beta; HNF-3-beta; HNF-3B; MGC19807; RONU; TCF-3B; Transcription factor 3B; WHN

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Gene Aliases: FOXA2; HNF3B; TCF3B

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UniProt ID: (Human) Q9Y261

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Entrez Gene ID: (Human) 3170

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding DNA binding transcription factor activity, sequence-specific DNA binding transcription factor binding protein domain specific binding transcription regulatory region DNA binding winged helix/forkhead transcription factor

Process(es)

positive regulation of transcription from RNA polymerase II promoter by glucose negative regulation of transcription from RNA polymerase II promoter by glucose cell fate specification transcription, DNA-templated regulation of transcription from RNA polymerase II promoter adult locomotory behavior negative regulation of epithelial to mesenchymal transition covalent chromatin modification regulation of blood coagulation endocrine pancreas development negative regulation of glucokinase activity positive regulation of embryonic development negative regulation of sequence-specific DNA binding transcription factor activity positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter regulation of insulin secretion involved in cellular response to glucose stimulus response to interleukin-6 dopaminergic neuron differentiation primitive streak formation positive regulation of cell-cell adhesion mediated by cadherin positive regulation of gastrulation negative regulation of detection of glucose

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FOXA2 Recombinant Rabbit Monoclonal Antibody (9H5L7)

FOXA2 Antibody (701698) in ICC/IF

Product Details

701698

Western Blot (WB)

Immunocytochemistry (ICC/IF)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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