Invitrogen

Phospho-EGFR (Tyr1068) Recombinant Rabbit Monoclonal Antibody (2H9L3)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

1 Reference

View all (279) EGFR antibodies

Datasheet

Protocols

Datasheet

Protocols

Phospho-EGFR (Tyr1068) Antibody in Immunocytochemistry (ICC/IF)

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Phospho-EGFR (Tyr1068) Antibody (701053) in ICC/IF

Immunofluorescent analysis of EGFR (pY1068) was performed on 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0. 25% Triton X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with EGFR (pY1068) Recombinant Rabbit Monoclonal Antibody (Product # 701053) at a dilution of 1:500 in 1% BSA and inc... View More

Phospho-EGFR (Tyr1068) Antibody in Western Blot (WB)

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Product Details

701053

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:5,000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100-1:1,000

Product Specifications

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Recombinant Monoclonal

Type

Antibody

Clone

2H9L3

Immunogen

Phosphopeptide corresponding to amino acids 1061-1072 of human EGFR

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2532358

Product Specific Information

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

EGFR, epidermal growth factor receptor, is a receptor tyrosine kinases that signals in response to various growth factors. Overexpression has been linked to numerous types of cancer and EGFR is the target of both biological and small molecular therapeutics. EGFR is encoded by the EGFR gene located on chromosome 7 in humans. EGFR belongs to the HER/ERbB family of proteins that includes three other receptor tyrosine kinases, ERbB2, ERbB3, ERbB4. EGFR is a transmembrane receptor and binding of its cognate ligands such as EGF (Epidermal Growth Factor) and TGF alpha (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of the tyrosine residues in the cytoplasmic domain. Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Evaluation of dimerization-inhibitory activities of cyclic peptides containing a β-hairpin loop sequence of the EGF receptor.

Bioorganic & medicinal chemistry

Mizuguchi T,Ohara N,Iida M,Ninomiya R,Wada S,Kiso Y,Saito K,Akaji K

701053 was used in western blot to study the ability of cyclic peptides containing the EGFR beta-hairpin loop sequence to inhibit EGFR dimerization

Mon Oct 01 00:00:00 EDT 2012

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