Invitrogen

IRAK4 Recombinant Rabbit Monoclonal Antibody (12H2L6)

IRAK4 Antibody in Immunocytochemistry (ICC/IF)

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IRAK4 Antibody (700026) in ICC/IF

Immunofluorescent analysis of IRAK-4 in HeLa cells using an IRAK-4 recombinant rabbit monoclonal antibody (Product # 700026) at a dilution of 5 µg/mL followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody at a dilution of 1:1000. Actin was stained using Alexa Fluor 568 Phalloidin at a dilution of 1:200. Panels: Hoechst only (top left), AF488 signal only (top right) and composite image with Phalloidin (bottom left).

IRAK4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

700026

Applications

Tested Dilution

Publications

Western Blot (WB)

2-3 µg/mL

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:50

Immunocytochemistry (ICC/IF)

4-6 µg/mL

ChIP assay (ChIP)

1 µL

in situ PLA (PLA)

View 1 publication 1 publication

In vitro Assay (IV)

- -

Product Specifications

Species Reactivity

Human

Published species

Mouse

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

12H2L6

Immunogen

A peptide corresponding to amino acids 41-52 of Q9NWZ3.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2532271

Product Specific Information

This antibody is predicted to react with mouse, rat, primate, ovine, equine, porcine, bovine, canine and Xenopus based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

IL-1 receptor associated kinases (IRAKs) are serine/threonine kinases involved in signaling via Toll/IL-1 pathways. Overexpression of IRAK-4 activates NF-kappa-B and MAPK pathways. IRAK-4 interacts with and phosphorylates IRAK-1. IRAK-4-deficient animals are completely resistant to the challenge with LPS. Animals and humans lacking IRAK-4 are impaired in their responses to viral and bacterial challenges. Members in IRAK/Pelle family play a central role in IL-1R/TLR mediated inflammatory responses and in innate immunity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

An Autochthonous Mouse Model of Myd88- and BCL2-Driven Diffuse Large B-cell Lymphoma Reveals Actionable Molecular Vulnerabilities.

Blood cancer discovery

Flümann R,Rehkämper T,Nieper P,Pfeiffer P,Holzem A,Klein S,Bhatia S,Kochanek M,Kisis I,Pelzer BW,Ahlert H,Hauer J,da Palma Guerreiro A,Ryan JA,Reimann M,Riabinska A,Wiederstein J,Krüger M,Deckert M,Altmüller J,Klatt AR,Frenzel LP,Pasqualucci L,Béguelin W,Melnick AM,Sander S,Montesinos-Rongen M,Brunn A,Lohneis P,Büttner R,Kashkar H,Borkhardt A,Letai A,Persigehl T,Peifer M,Schmitt CA,Reinhardt HC,Knittel G

700026 was used in Proximity Ligation Assay (PLA) to employ immune phenotyping, RNA-Seq and whole exome sequencing to characterize a Myd88 and Bcl2-driven mouse model of ABC-DLBCL.

Fri Jan 01 00:00:00 EST 2021

Talin1 controls dendritic cell activation by regulating TLR complex assembly and signaling.

The Journal of experimental medicine

Lim TJF,Bunjamin M,Ruedl C,Su IH

700026 was used in Western Blotting to investigate the role and mechanism of Talin in regulating skin dendritic cells (DCs) during inflammatory responses.

Mon Aug 03 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: interleukin-1; Interleukin-1 receptor-associated kinase 4; IRAK-4; Renal carcinoma antigen NY-REN-64

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Gene Aliases: IPD1; IRAK-4; IRAK4; NY-REN-64; REN64

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UniProt ID: (Human) Q9NWZ3

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Entrez Gene ID: (Human) 51135

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Function(s)

magnesium ion binding protein kinase activity protein serine/threonine kinase activity interleukin-1 receptor binding protein binding ATP binding

Process(es)

cytokine production toll-like receptor signaling pathway neutrophil mediated immunity MyD88-dependent toll-like receptor signaling pathway JNK cascade cytokine-mediated signaling pathway toll-like receptor 9 signaling pathway positive regulation of I-kappaB kinase/NF-kappaB signaling innate immune response positive regulation of smooth muscle cell proliferation neutrophil migration

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IRAK4 Recombinant Rabbit Monoclonal Antibody (12H2L6)

IRAK4 Antibody (700026) in ICC/IF

Product Details

700026

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ChIP assay (ChIP)

in situ PLA (PLA)

In vitro Assay (IV)

Species Reactivity

Published species

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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