CaptureSelect™ LambdaXP RoboColumn™ Pre-packed columns
CaptureSelect RoboColumn™ are small chromatography columns used for fully automated and parallel chromatographic separations on robotic liquid handling workstations.
The CaptureSelect LambdaXP affinity matrix in this RoboColumn recognizes the constant domain of the human lambda light chain enabling purification of all IgG isotypes, including IgG, IgA, IgM, IgD, and IgE, directly from complex source materials in a robust and efficient purification process with excellent purity obtained in a single step.
Features of the LambdaXP affinity matrix include:
- Efficient elution of Fab fragments and immunoglobulins containing a lambda light chain; no cross binding to the Fc-region or kappa light chains
- One-step purification from crude material with high purity and yield
- Human specific
- Non-animal-derived
Free of animal components
CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).
Characteristics
- Column size: 200 μL, 1 strip, 8 columns (8-row)
- Column dimension: 5-mm diameter, 10-mm bed height
- Matrix: agarose-based, epoxide-activated
- Average particle size: 65 ± 10 μm
- Ligand:CaptureSelect LambdaXP affinity ligand
- Ligand coupling: epoxide coupling
- Binding capacity: >30 g IgG/L resin depending on flow rate, column height, and contact time
- Elution conditions: 25 mM sodium acetate, pH 3.5
- Formulation buffer: 20% (v/v) ethanol
- Operating pressure: 2 bar (maximum)
- Flow rate: 16–150 cm/hr
RoboColumn is a trademark of Repligen.