Invitrogen

ERK5 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

1 Reference
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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

44-688G

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
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Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

The antiserum was produced against a chemically synthesized peptide derived from residues 334-349 of the human ERK5/BMK1 protein. Full-length ERK5/BMK1 has a Mr of ~88 kDa, however, truncated versions of ~30-35 kDa have also been observed.
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533723

Target Information

MAPK7 (ERK5) serine/threonine kinase is a member of the MAP kinase family and is involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. MAPK7 is activated by mitogen-activated protein kinase 5 (MAP2K5/MEK5) and is involved in downstream signaling processes of various receptors. In response to extracelluar signals, MAPK7 translocates to the cell nucleus where it regulates gene expression by phosphorylating and activating different transcription factors. Gene deletion of MAPK7 in mice results in defective blood vessel and cardiac development leading to embryonic lethality. MAPK7 has been shown to be critical for endothelial function and maintenance of blood vessel integrity. Four alternatively spliced transcript variants of this gene encoding two distinct isoforms have been reported. ERK5 is expressed in many adult tissues, abundantly in heart, placenta, lung, kidney and skeletal muscle, but is not detectable in liver.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Big MAP kinase 1; BMK-1; BMK1 kinase; BNhp-1; BNhp1 kinase; ERK-5; extracellular signal regulated kinase 5; Extracellular signal-regulated kinase 5; extracellular-signal-regulated kinase 5; MAP kinase 7; MAPK 7; mitogen activated protein kinase 7; Mitogen-activated protein kinase 7

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Gene Aliases: BMK-1; BMK1; ERK-5; ERK4; ERK5; Erk5-T; MAPK7; PRKM7

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UniProt ID: (Human) Q13164, (Mouse) Q9WVS8

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Entrez Gene ID: (Human) 5598, (Mouse) 23939, (Rat) 114509

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Function(s)
protein serine/threonine kinase activity MAP kinase activity protein binding ATP binding mitogen-activated protein kinase binding nucleotide binding protein kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups non-receptor serine/threonine protein kinase translation factor translation initiation factor translational protein
Process(es)
MAPK cascade cell cycle signal transduction peptidyl-serine phosphorylation cAMP-mediated signaling cell differentiation negative regulation of cAMP catabolic process negative regulation of heterotypic cell-cell adhesion positive regulation of transcription from RNA polymerase II promoter in response to stress negative regulation of MAP kinase activity regulation of angiogenesis positive regulation of transcription from RNA polymerase II promoter negative regulation of inflammatory response positive regulation of protein metabolic process negative regulation of cyclic-nucleotide phosphodiesterase activity negative regulation of NFAT protein import into nucleus negative regulation of response to cytokine stimulus cellular response to hydrogen peroxide negative regulation of ERK5 cascade cellular response to growth factor stimulus cellular response to laminar fluid shear stress cellular response to transforming growth factor beta stimulus negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway negative regulation of endothelial cell apoptotic process negative regulation of extrinsic apoptotic signaling pathway in absence of ligand protein phosphorylation phosphorylation negative regulation of apoptotic process protein autophosphorylation ERK5 cascade
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